##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939756_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2385247 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.444889355274317 31.0 31.0 34.0 30.0 34.0 2 31.679793748823496 31.0 31.0 34.0 30.0 34.0 3 31.72077986053436 31.0 31.0 34.0 30.0 34.0 4 35.52165750549104 37.0 35.0 37.0 33.0 37.0 5 35.26043005189819 37.0 35.0 37.0 33.0 37.0 6 35.27591901383798 37.0 35.0 37.0 32.0 37.0 7 35.188388456206006 37.0 35.0 37.0 32.0 37.0 8 35.14851250205953 37.0 35.0 37.0 32.0 37.0 9 36.74813384106552 39.0 35.0 39.0 32.0 39.0 10-11 36.618574722030885 39.0 35.0 39.0 32.0 39.0 12-13 36.53837527098871 38.0 35.0 39.0 32.0 39.0 14-15 37.59308889184223 39.0 36.0 41.0 32.0 41.0 16-17 37.50903554223106 39.0 36.0 41.0 32.0 41.0 18-19 37.48325561252147 39.0 36.0 41.0 32.0 41.0 20-21 37.399098500071474 39.0 36.0 41.0 32.0 41.0 22-23 37.27624560475289 39.0 36.0 40.5 31.0 41.0 24-25 37.12981422888279 39.0 36.0 40.0 31.0 41.0 26-27 36.83382287033586 39.0 36.0 40.0 30.5 41.0 28-29 36.612484157825165 38.5 35.0 40.0 30.0 41.0 30-31 36.357082935226416 38.0 35.0 40.0 30.0 41.0 32-33 36.34420502363068 38.0 35.0 40.0 30.0 41.0 34-35 36.201336381515205 38.0 35.0 40.0 30.0 41.0 36-37 36.247926105765984 38.0 35.0 40.0 30.0 41.0 38-39 36.15710595171066 38.0 35.0 40.0 30.0 41.0 40-41 36.08182863242256 38.0 35.0 40.0 29.5 41.0 42-43 35.86950638655033 38.0 35.0 40.0 29.0 41.0 44-45 35.637606294023215 38.0 34.0 40.0 27.5 41.0 46-47 35.374526202108214 38.0 34.0 40.0 27.0 41.0 48-49 35.08489351417275 38.0 34.0 40.0 26.0 41.0 50-51 34.86789816735961 38.0 33.5 40.0 26.0 41.0 52-53 34.63277807287882 38.0 33.0 40.0 25.5 41.0 54-55 34.370494334549 38.0 33.0 40.0 24.5 41.0 56-57 34.011789554708585 37.0 33.0 40.0 23.5 41.0 58-59 33.65199306403069 37.0 32.0 40.0 23.0 41.0 60-61 33.34162541657112 36.0 32.0 39.0 23.0 41.0 62-63 33.0556585963634 36.0 31.5 39.0 22.0 40.5 64-65 32.6262007666292 35.5 31.0 39.0 21.5 40.0 66-67 31.74783512986286 35.0 30.0 38.5 16.0 40.0 68-69 29.915841839440528 34.0 27.0 37.5 9.5 40.0 70-71 29.277646298265964 34.0 26.0 37.0 9.0 39.0 72-73 29.82284664858608 34.0 28.0 36.5 10.0 39.0 74-75 29.894707130959603 34.0 28.0 36.0 11.0 39.0 76-77 29.416176815231296 33.0 28.5 35.0 11.0 38.0 78-79 29.937010297046804 34.0 29.0 35.5 10.5 38.0 80-81 29.75113269191828 34.0 29.0 35.0 7.5 37.0 82-83 29.307784686449665 34.0 29.0 35.0 7.0 37.0 84-85 28.945484681460663 34.0 29.0 35.0 3.5 36.0 86-87 28.56397723170808 33.0 28.0 35.0 2.0 36.0 88-89 28.108511613262692 33.0 27.0 35.0 2.0 35.5 90-91 27.624306413549625 33.0 26.0 35.0 2.0 35.0 92-93 27.144425923185313 32.5 25.5 35.0 2.0 35.0 94-95 26.346764087744372 32.0 24.5 34.0 2.0 35.0 96-97 25.836809772740516 32.0 24.0 34.0 2.0 35.0 98-99 25.172056814241877 32.0 21.0 34.0 2.0 35.0 100-101 23.68367364050767 30.5 9.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.11872051240275994 1101 2 0.2799216105888007 1101 3 0.3542620270681631 1101 4 0.14394868116948345 1101 5 0.18507780658607942 1101 6 0.24368574896496398 1101 7 0.18738891162585247 1101 8 0.20121228171058902 1101 9 0.38921668676137244 1101 10-11 0.4128132491209584 1101 12-13 0.38701075250752126 1101 14-15 0.3323363386926772 1101 16-17 0.2281698005116226 1101 18-19 0.3004066126267517 1101 20-21 0.38246086998471185 1101 22-23 0.32596677969296906 1101 24-25 0.3382361459033234 1101 26-27 0.4028928672335681 1101 28-29 0.42219773323992627 1101 30-31 0.3431908528243426 1101 32-33 0.2001922387485493 1101 34-35 0.25545258384934755 1101 36-37 0.5559215698702857 1101 38-39 0.46913421290386736 1101 40-41 0.5311584232982725 1101 42-43 0.23645168549825257 1101 44-45 0.21579449392185523 1101 46-47 0.35981150995016975 1101 48-49 0.44135191567900733 1101 50-51 0.3026092409340322 1101 52-53 0.08952164289478759 1101 54-55 0.013682904032130239 1101 56-57 -0.03649304570634371 1101 58-59 0.027994298237281612 1101 60-61 0.08726245597565452 1101 62-63 0.12987630429648078 1101 64-65 0.025839143007324594 1101 66-67 0.011341047225979395 1101 68-69 -8.384014447167676 1101 70-71 -6.547382215363044 1101 72-73 -1.5575134639440265 1101 74-75 -0.29499172196294055 1101 76-77 -0.0314480560875019 1101 78-79 -0.4071147946045528 1101 80-81 -0.2660453548731887 1101 82-83 -0.2313177698850737 1101 84-85 -0.1256894268930182 1101 86-87 -0.23038388964450007 1101 88-89 -0.17231183440490128 1101 90-91 -0.5108970310289145 1101 92-93 -0.8252257738522388 1101 94-95 -0.8030610123175776 1101 96-97 -1.2242178323638484 1101 98-99 -0.8305762617334089 1101 100-101 -0.9543549593006269 1102 1 0.18723929256426786 1102 2 0.13911473549405784 1102 3 0.23904451098570334 1102 4 0.06587664087907541 1102 5 0.20489609798212882 1102 6 0.1583425682071038 1102 7 0.12354567126245541 1102 8 0.16662763668735892 1102 9 0.20799026222567107 1102 10-11 0.3136612667583094 1102 12-13 0.24410351786625029 1102 14-15 0.330757435670165 1102 16-17 0.26196687969279964 1102 18-19 0.21511319513921734 1102 20-21 0.16367182283452308 1102 22-23 0.2913249207064439 1102 24-25 0.15898222334342904 1102 26-27 0.24432519580876289 1102 28-29 0.0699541732141995 1102 30-31 -0.0017430254949886148 1102 32-33 -0.07973498729774775 1102 34-35 -0.02541710886567472 1102 36-37 0.012335291949504779 1102 38-39 0.1852001697494572 1102 40-41 -0.03268454111353947 1102 42-43 0.11112698245895558 1102 44-45 0.12554673354497226 1102 46-47 0.11091917963832287 1102 48-49 0.13893334715397287 1102 50-51 0.22108053902503855 1102 52-53 0.10424264847710418 1102 54-55 -0.09971048004685912 1102 56-57 -0.15310209368070815 1102 58-59 -0.3491874670287345 1102 60-61 -0.1548433968472267 1102 62-63 -0.2120338804512727 1102 64-65 -0.1587226164305875 1102 66-67 0.11398050580159946 1102 68-69 -4.604228140400753 1102 70-71 0.2744483518315377 1102 72-73 0.48836633963730947 1102 74-75 0.2619904419979271 1102 76-77 0.10180133277170711 1102 78-79 0.004631563202377009 1102 80-81 -0.08825130821603366 1102 82-83 -0.13381809366827468 1102 84-85 -0.16405221713890583 1102 86-87 -0.1416026280085383 1102 88-89 -0.04892182797338762 1102 90-91 -0.12189432743918971 1102 92-93 -0.1730613751397172 1102 94-95 -0.010128647684712888 1102 96-97 -0.5388761417445807 1102 98-99 -0.08353234716507885 1102 100-101 -0.0017573717798171629 1103 1 0.29346723472403013 1103 2 0.32127615673121923 1103 3 0.30000264251583886 1103 4 0.20241503532994898 1103 5 0.340787656261341 1103 6 0.23127879742840207 1103 7 0.20562046005954215 1103 8 0.24272972923871095 1103 9 0.4295054868595187 1103 10-11 0.46237108296962504 1103 12-13 0.4133180407191901 1103 14-15 0.40069532486204196 1103 16-17 0.3945733823586366 1103 18-19 0.35231623192603223 1103 20-21 0.2742768108862208 1103 22-23 0.20218798912280533 1103 24-25 0.2760050617477674 1103 26-27 0.2716442125014922 1103 28-29 0.14074784846465604 1103 30-31 0.2748735561866127 1103 32-33 0.28051024355735166 1103 34-35 0.30943217103850174 1103 36-37 0.40090758643003 1103 38-39 0.39349195536613735 1103 40-41 0.2540495757879242 1103 42-43 0.32406545016947774 1103 44-45 0.4086959971692252 1103 46-47 0.2868012025348676 1103 48-49 0.3746618881822812 1103 50-51 0.2577456116160448 1103 52-53 0.32335767484476463 1103 54-55 0.27673181925950274 1103 56-57 0.41336868095284274 1103 58-59 0.4731168250563229 1103 60-61 0.2089594329520139 1103 62-63 0.2515852188039176 1103 64-65 0.17478684045959625 1103 66-67 0.4720057803799165 1103 68-69 -4.2071532207828675 1103 70-71 0.5755011685486906 1103 72-73 1.0322774309153395 1103 74-75 0.727298219730482 1103 76-77 0.6071253758902415 1103 78-79 0.4632322312605872 1103 80-81 0.6947166232454975 1103 82-83 0.4985823662395106 1103 84-85 0.4607203000276918 1103 86-87 0.4350078487024511 1103 88-89 0.23274879723374298 1103 90-91 0.11728606572091849 1103 92-93 0.032917789467731495 1103 94-95 0.024696575728139436 1103 96-97 -0.1847751277380354 1103 98-99 0.2396121051609157 1103 100-101 0.022642887536076017 1104 1 0.22565365315781705 1104 2 0.22219512483378168 1104 3 0.2057439254996254 1104 4 0.15692583079577105 1104 5 0.1957865649512911 1104 6 0.15618532743091862 1104 7 0.05435850879716497 1104 8 0.1596874119197622 1104 9 0.23742712229392993 1104 10-11 0.21198062073603552 1104 12-13 0.1158736904455111 1104 14-15 0.26574597865597127 1104 16-17 0.31953802107832274 1104 18-19 0.22708946620053183 1104 20-21 0.22960679753538926 1104 22-23 0.21066654700617704 1104 24-25 0.15359302558997 1104 26-27 0.07072942539199545 1104 28-29 0.047638550219538445 1104 30-31 0.1409092236006586 1104 32-33 0.2442404830503051 1104 34-35 0.24845628473884318 1104 36-37 0.22428047148532215 1104 38-39 0.15422541493239095 1104 40-41 0.2575715309620037 1104 42-43 0.27676460008146364 1104 44-45 0.22122770893865606 1104 46-47 0.3139411623406474 1104 48-49 0.3607965813540872 1104 50-51 0.45708047939746166 1104 52-53 0.3008862544126103 1104 54-55 0.3265781146161899 1104 56-57 0.2541777243699741 1104 58-59 0.10487125478304193 1104 60-61 0.2347721046207525 1104 62-63 0.3050895287699902 1104 64-65 0.4139492103881963 1104 66-67 0.4499414752893145 1104 68-69 -3.920376780607917 1104 70-71 0.7287246433365908 1104 72-73 0.9403416452996574 1104 74-75 0.5553098811579176 1104 76-77 0.4537201342004984 1104 78-79 0.20384767343957932 1104 80-81 0.039090849053138754 1104 82-83 0.09149147286759174 1104 84-85 0.07096777229849494 1104 86-87 0.07562740101021603 1104 88-89 0.12097809706622087 1104 90-91 0.19172191511446002 1104 92-93 0.07905433928391048 1104 94-95 0.18435736254851776 1104 96-97 -0.299301385791761 1104 98-99 -0.023570778562124417 1104 100-101 0.046860404054541505 1105 1 0.22279594157283 1105 2 0.2814681837097517 1105 3 0.2174799891154784 1105 4 0.09499802812197089 1105 5 0.20299540910673386 1105 6 0.19430175914493475 1105 7 0.04357348451929255 1105 8 0.15021240605020125 1105 9 0.1973453227029296 1105 10-11 0.2383274727788134 1105 12-13 0.209173626816046 1105 14-15 0.24033111113796224 1105 16-17 0.3144072574226513 1105 18-19 0.1711894495275743 1105 20-21 0.3327670893462482 1105 22-23 0.3267395371487112 1105 24-25 0.3550693482832372 1105 26-27 0.3756288942390711 1105 28-29 0.3969049399108826 1105 30-31 0.3403908306019474 1105 32-33 0.2912707393715692 1105 34-35 0.3328484361973878 1105 36-37 0.2307036448603057 1105 38-39 0.26592413451700025 1105 40-41 0.2655692784108723 1105 42-43 0.3019175489839441 1105 44-45 0.28917470593412276 1105 46-47 0.35417786735512635 1105 48-49 0.2585705708535144 1105 50-51 0.4242041213166843 1105 52-53 0.2830964596261438 1105 54-55 0.40964927413559593 1105 56-57 0.24075386366066454 1105 58-59 0.42461808096571474 1105 60-61 0.4016099243417983 1105 62-63 0.4127454805068709 1105 64-65 0.38607295438678335 1105 66-67 0.9249189327647542 1105 68-69 2.162901004542256 1105 70-71 2.3891286564163607 1105 72-73 1.4159151611508776 1105 74-75 0.8883512675316858 1105 76-77 0.5584563611433211 1105 78-79 0.47842821235052924 1105 80-81 0.335499101828848 1105 82-83 0.4286567801774943 1105 84-85 0.34674051302713593 1105 86-87 0.19546499275403661 1105 88-89 0.37174539356251657 1105 90-91 0.3079269278913941 1105 92-93 0.19810906742957002 1105 94-95 0.6113163504657244 1105 96-97 0.4729691823479101 1105 98-99 0.582118018909032 1105 100-101 0.5179228630101349 1106 1 0.3888693793123785 1106 2 0.39919047565738097 1106 3 0.31212845615268847 1106 4 0.2559514558834195 1106 5 0.27936109589329305 1106 6 0.37798705418732936 1106 7 0.2546444926966487 1106 8 0.30446024218077383 1106 9 0.4153178465633047 1106 10-11 0.3908426542943957 1106 12-13 0.42812161339758603 1106 14-15 0.561765566227443 1106 16-17 0.48863155148543314 1106 18-19 0.5526392875134505 1106 20-21 0.5112902793224237 1106 22-23 0.466357771077881 1106 24-25 0.5765048427576005 1106 26-27 0.5742665204918538 1106 28-29 0.5607208336649592 1106 30-31 0.7054178676014473 1106 32-33 0.6486162023265933 1106 34-35 0.6182554723134146 1106 36-37 0.5378278487564359 1106 38-39 0.7957121259836484 1106 40-41 0.7337045959299715 1106 42-43 0.5947834709313327 1106 44-45 0.8593656669234022 1106 46-47 0.9016335830625692 1106 48-49 0.7764641014621034 1106 50-51 0.9011973367317978 1106 52-53 0.7326517328154623 1106 54-55 0.786044583391579 1106 56-57 0.9824069533621795 1106 58-59 0.9313184756721284 1106 60-61 0.8092135445193804 1106 62-63 0.9124705632029375 1106 64-65 0.901142216397524 1106 66-67 1.4914621724754937 1106 68-69 3.1025319709363437 1106 70-71 3.1580066144408008 1106 72-73 2.1614662040436983 1106 74-75 1.6144073475435299 1106 76-77 1.1306042240380627 1106 78-79 1.0142677625047476 1106 80-81 0.9735813847338228 1106 82-83 1.0329964719479037 1106 84-85 1.058567413867216 1106 86-87 1.1866993402671788 1106 88-89 1.3224954396852198 1106 90-91 1.04049709286776 1106 92-93 1.1829704264772865 1106 94-95 1.576390159785369 1106 96-97 1.2747405540501404 1106 98-99 1.33002482765961 1106 100-101 1.5365696878192097 1107 1 0.0988685612097342 1107 2 0.06033028939922502 1107 3 0.12110097101056994 1107 4 0.06375118435618532 1107 5 0.08469150062627762 1107 6 0.07724734441701031 1107 7 0.10381018333579561 1107 8 0.07571977851333855 1107 9 0.0031157938505685934 1107 10-11 0.11060211140562615 1107 12-13 0.09778393376451078 1107 14-15 0.1045510400383236 1107 16-17 0.08517465358567478 1107 18-19 0.06890296096001691 1107 20-21 0.07657487030733989 1107 22-23 0.05515064509317824 1107 24-25 0.1570118385707815 1107 26-27 0.05962222427242381 1107 28-29 0.16924007823519105 1107 30-31 0.17557265469282868 1107 32-33 0.09265073247160416 1107 34-35 0.13278093653487844 1107 36-37 0.02959815038777691 1107 38-39 0.0316348059636411 1107 40-41 -0.020593290776275808 1107 42-43 -0.0060544111562563785 1107 44-45 0.023447284571226135 1107 46-47 -0.016952776991658425 1107 48-49 -0.08183197713373858 1107 50-51 0.019863643019078836 1107 52-53 -0.09257591201200199 1107 54-55 -0.028855033342864544 1107 56-57 -0.0484696902215731 1107 58-59 0.012136143375407471 1107 60-61 0.05084067818803817 1107 62-63 0.15833500255926225 1107 64-65 0.0037491160059701656 1107 66-67 0.6753871804235132 1107 68-69 1.8406078660079466 1107 70-71 2.2011925960966607 1107 72-73 1.1339813208200802 1107 74-75 0.6093511625322101 1107 76-77 0.20776036462330438 1107 78-79 0.2783052129655239 1107 80-81 0.29346806198404707 1107 82-83 0.38690223895019926 1107 84-85 0.2809605919267426 1107 86-87 0.20517344421178407 1107 88-89 0.4208101482387434 1107 90-91 0.3675628797116346 1107 92-93 0.24985005872461485 1107 94-95 0.6374527197838766 1107 96-97 0.1742666758604443 1107 98-99 0.4883654876716861 1107 100-101 0.5235175850365223 1108 1 -0.07163016308020076 1108 2 -0.16540387213553132 1108 3 -0.0876616641363519 1108 4 -0.13745623161398868 1108 5 0.03359953419977302 1108 6 0.02541577165226272 1108 7 -0.010725815931003524 1108 8 0.05419481963269135 1108 9 -0.10275828681530186 1108 10-11 -0.09369677036477952 1108 12-13 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2316 66-67 -0.18189743753969623 2316 68-69 1.1096654121207585 2316 70-71 1.4519073246315202 2316 72-73 0.47271866666778806 2316 74-75 0.03395839501740028 2316 76-77 -0.3287279499532083 2316 78-79 -0.49411180028607404 2316 80-81 -0.6822086684241455 2316 82-83 -0.5937111905143944 2316 84-85 -0.6349740208780688 2316 86-87 -0.7609507594111413 2316 88-89 -0.8652963819827448 2316 90-91 -0.7665298987471374 2316 92-93 -0.6867719352527573 2316 94-95 -0.5410776409309968 2316 96-97 -0.42624232813551544 2316 98-99 -0.6983352150310331 2316 100-101 -0.8658001514259688 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 10.0 4 42.0 5 75.0 6 224.0 7 563.0 8 1328.0 9 2269.0 10 3537.0 11 5655.0 12 7860.0 13 9995.0 14 12397.0 15 15077.0 16 18262.0 17 21323.0 18 20407.0 19 17992.0 20 17839.0 21 18422.0 22 20033.0 23 22897.0 24 27308.0 25 33342.0 26 41372.0 27 51329.0 28 63172.0 29 76051.0 30 90874.0 31 108812.0 32 129834.0 33 157838.0 34 197479.0 35 250936.0 36 320214.0 37 366368.0 38 231232.0 39 22873.0 40 3.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.598852690683565 19.837464513504788 14.905544070348176 23.658138725463473 2 15.637130968011673 20.798976453996833 39.521212957367766 24.04267962062373 3 18.99757928532319 24.624462214798807 30.43673482735114 25.94122367252686 4 13.222907313162954 17.35824424053358 37.15527155049351 32.263576895809955 5 13.448376625146159 37.57120331772768 34.84050079509585 14.139919262030306 6 31.929355743870552 36.891022187639265 17.260183117303995 13.919438951186189 7 27.764000960906777 32.68726467321833 21.253815642572864 18.294918723302033 8 25.891280861059673 35.8993009948236 19.78369535733616 18.42572278678057 9 25.873001831676135 16.508604769233543 20.25945321386003 37.358940185230296 10-11 24.73200888629144 26.74194747965305 27.72366970800089 20.802373926054617 12-13 25.430259423866797 23.91020720285991 28.132956461112833 22.526576912160458 14-15 22.24010762826659 25.52827862271706 26.15641063587964 26.075203113136713 16-17 21.955493264917976 28.518181336048933 27.01895767481143 22.507367724221663 18-19 21.899304348773942 27.293148256763345 29.052609645877347 21.75493774858537 20-21 22.49861335115399 26.541863379348136 29.195571779358698 21.763951490139178 22-23 22.197805929532667 26.411960690024976 29.096672168542714 22.29356121189965 24-25 22.11369151332107 26.606292689552934 29.17806312477885 22.101952672347146 26-27 21.940719974124182 26.91137594835904 28.877112288771123 22.270791788745655 28-29 21.912750299130572 27.22302018324315 28.86337509841752 22.00085441920875 30-31 22.20356349735729 26.79173718714377 28.827883639430986 22.176815676067953 32-33 21.8864456578612 26.843002468572656 28.807211519111313 22.463340354454832 34-35 22.325628879365553 27.040182832379962 28.680425074382565 21.953763213871916 36-37 22.35332875887351 26.697386892284065 28.762734519755163 22.18654982908727 38-39 22.006911891739573 26.99750251207042 28.764669830333222 22.230915765856782 40-41 22.33918300454726 26.807435090892685 28.77047787120371 22.082904033356346 42-43 22.06175948818308 26.809617719528926 28.64153132104517 22.487091471242824 44-45 22.121574932152257 26.997694834339036 28.65931934454904 22.22141088895967 46-47 22.331147781752566 27.040189670908386 28.255300399693674 22.373362147645373 48-49 22.07566643020514 27.158330401168417 28.2783020006072 22.48770116801924 50-51 22.14734023112078 27.286712212709336 28.195430037476665 22.370517518693223 52-53 22.310906318883482 27.13748041789697 28.151281657558417 22.400331605661126 54-55 21.994385486835846 27.18554489387211 28.21586511073394 22.604204508558105 56-57 22.073822508593082 27.244308602812527 28.07914611008876 22.602722778505637 58-59 22.06422816554749 27.052477321184853 28.110682261780717 22.772612251486937 60-61 21.93571731652626 27.218317772369964 28.211995834011173 22.633969077092598 62-63 21.902486563492907 27.138589455515145 28.302559177963936 22.656364803028005 64-65 21.734225948088728 27.060272459229118 28.202014357723016 23.003487234959138 66-67 21.561206880808374 27.201565434494345 28.171229683472653 23.06599800122462 68-69 21.577157225377082 26.94494577999862 28.55851904729035 22.919377947333953 70-71 21.494122055793916 26.915810450581017 28.261390561833878 23.328676931791186 72-73 21.48596675375526 27.01517222901942 28.115457515386083 23.383403501839233 74-75 21.691705296777382 27.218166538202503 27.687200524033145 23.402927640986974 76-77 21.694927195571132 26.98452405265756 27.84141924288091 23.4791295088904 78-79 21.803708056287054 27.08415177195718 27.70348882304578 23.408651348709984 80-81 21.817569332382476 27.094330778844487 27.52269923063475 23.565400658138287 82-83 21.734173330549552 27.111459363101332 27.583779618538294 23.570587687810818 84-85 21.66423504553001 27.09251479934933 27.48926732739682 23.753982827723835 86-87 21.629258175502013 27.00081606710994 27.5747881589983 23.79513759838975 88-89 21.68512355237526 26.99785158202926 27.346264647613545 23.970760217981937 90-91 21.56843056952753 27.004695351373652 27.46940915378654 23.95746492531228 92-93 21.592321932463477 26.760980863736478 27.506541949497805 24.140155254302236 94-95 21.245765577681354 26.60681485012012 27.758049073566717 24.38937049863181 96-97 21.450600330250825 26.33201026142481 27.787361406610216 24.430028001714152 98-99 21.380044296807057 26.4148645056109 27.5599394839531 24.64515171362894 100-101 21.16901450969507 26.399481598942735 27.700494669659786 24.731009221702404 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 392.0 1 268.0 2 187.0 3 271.5 4 275.5 5 281.5 6 456.0 7 926.0 8 1441.5 9 1801.5 10 1765.0 11 1561.0 12 1629.5 13 1732.0 14 1888.5 15 2031.0 16 2182.5 17 2495.0 18 2899.0 19 3286.0 20 3767.0 21 4540.5 22 5669.0 23 7062.0 24 8733.5 25 10986.0 26 14113.5 27 18054.0 28 21596.5 29 24983.5 30 28974.5 31 34357.5 32 40638.5 33 44726.5 34 47768.0 35 51964.5 36 57257.0 37 61872.0 38 65521.5 39 68765.5 40 71423.0 41 74076.0 42 75960.5 43 78392.0 44 81929.0 45 85219.5 46 87199.5 47 88818.5 48 90092.5 49 89613.0 50 88890.5 51 87795.5 52 85958.0 53 84722.0 54 82188.5 55 76023.0 56 68802.5 57 61668.0 58 55517.0 59 49041.5 60 40648.0 61 33292.0 62 26921.0 63 20224.5 64 14314.5 65 10220.5 66 7424.0 67 5603.5 68 4100.5 69 2906.5 70 2162.0 71 1488.0 72 1027.0 73 824.5 74 631.0 75 427.0 76 302.5 77 203.5 78 121.0 79 65.0 80 29.0 81 21.0 82 15.5 83 8.5 84 4.0 85 2.0 86 1.5 87 1.5 88 1.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022513391694864306 2 0.024986930074746973 3 4.1924379320045264E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 5.240547415005658E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.2577313796013578E-4 26-27 3.5635722422038473E-4 28-29 4.1924379320045264E-5 30-31 2.5154627592027156E-4 32-33 0.0026412358971628514 34-35 0.004674568294185047 36-37 0.10279857809275099 38-39 0.09443466441840195 40-41 0.10447555326555279 42-43 0.10564943588651406 44-45 0.09801919885026583 46-47 0.0910597518831383 48-49 0.09204497479715937 50-51 0.09072435684857795 52-53 0.07097797418883663 54-55 0.0597422405310645 56-57 0.0323236964557549 58-59 0.01308040634785412 60-61 0.009181439071089913 62-63 0.0019914080177021497 64-65 0.005009963328745409 66-67 0.13786832139396885 68-69 0.39287335860814415 70-71 0.375160308345425 72-73 0.16583188240043903 74-75 0.0278797122478301 76-77 0.029682460558592045 78-79 0.004863228001125251 80-81 0.001425428896881539 82-83 4.1924379320045264E-5 84-85 2.934706552403168E-4 86-87 0.0011109960519811995 88-89 1.467353276201584E-4 90-91 6.498278794607015E-4 92-93 0.002662198086822874 94-95 0.0756735046726817 96-97 0.016476281072777786 98-99 0.017817861211019238 100-101 0.009265287829730002 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2385247.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.344422733035074 #Duplication Level Percentage of deduplicated Percentage of total 1 75.05548981384186 43.79069226133797 2 14.348913437891284 16.743581427601136 3 4.786765301790681 8.37843174874499 4 2.0789775750420443 4.851869859630127 5 1.1135311302424111 3.2484165494628776 6 0.65821753452293 2.304199325070116 7 0.4163632952063755 1.7004733274228172 8 0.29128523162290565 1.3595894951757475 9 0.20096909135435717 1.0552883060027303 >10 0.9685296038189116 10.138146878425195 >50 0.05535862912875438 2.195329759970233 >100 0.023151569595789755 2.483577408949934 >500 0.0017278489000411678 0.69273509868027 >1k 5.759496333461702E-4 0.5302204190292721 >5k 1.4398740833654255E-4 0.5274481344965991 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6715 0.2815222071341039 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5842 0.2449222239877044 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3783 0.15859992696773123 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.2577313796013578E-4 2 4.1924379320045264E-5 0.0 0.0 0.0 1.2577313796013578E-4 3 4.1924379320045264E-5 0.0 0.0 0.0 1.2577313796013578E-4 4 4.1924379320045264E-5 0.0 0.0 1.6769751728018105E-4 1.2577313796013578E-4 5 4.1924379320045264E-5 0.0 0.0 1.6769751728018105E-4 1.2577313796013578E-4 6 4.1924379320045264E-5 0.0 0.0 2.096218966002263E-4 1.6769751728018105E-4 7 4.1924379320045264E-5 0.0 0.0 2.096218966002263E-4 1.6769751728018105E-4 8 4.1924379320045264E-5 0.0 0.0 2.096218966002263E-4 1.6769751728018105E-4 9 4.1924379320045264E-5 0.0 0.0 2.934706552403168E-4 2.096218966002263E-4 10-11 6.28865689800679E-5 0.0 0.0 4.192437932004526E-4 2.096218966002263E-4 12-13 8.384875864009053E-5 0.0 0.0 5.030925518405431E-4 2.9347065524031686E-4 14-15 8.384875864009053E-5 0.0 0.0 5.030925518405431E-4 3.353950345603621E-4 16-17 8.384875864009053E-5 0.0 0.0 5.030925518405431E-4 3.9828160354043E-4 18-19 8.384875864009053E-5 0.0 0.0 6.707900691207242E-4 4.6116817252049786E-4 20-21 8.384875864009053E-5 0.0 0.0 7.756010174208373E-4 5.450169311605884E-4 22-23 8.384875864009053E-5 0.0 0.0 9.432985347010183E-4 6.079035001406562E-4 24-25 1.2577313796013578E-4 0.0 0.0 0.001425428896881539 6.707900691207242E-4 26-27 1.2577313796013578E-4 0.0 0.0 0.0021800677246423534 7.9656320708086E-4 28-29 1.4673532762015843E-4 0.0 0.0 0.006707900691207242 8.384875864009052E-4 30-31 1.6769751728018105E-4 0.0 0.0 0.022429542936224216 8.594497760609278E-4 32-33 1.6769751728018105E-4 0.0 0.0 0.05913433703092384 8.804119657209505E-4 34-35 1.6769751728018105E-4 0.0 0.0 0.11919101040688868 8.804119657209505E-4 36-37 2.096218966002263E-4 0.0 0.0 0.21855178939539596 8.804119657209505E-4 38-39 2.096218966002263E-4 0.0 0.0 0.36373591498071267 9.432985347010183E-4 40-41 2.096218966002263E-4 0.0 0.0 0.5205121314480219 0.0010061851036810862 42-43 2.096218966002263E-4 0.0 0.0 0.6783993439673124 0.0010271472933411087 44-45 2.096218966002263E-4 0.0 0.0 0.8663672986487354 0.0010900338623211767 46-47 2.3058408626024893E-4 0.0 0.0 1.0907046523902975 0.001131958241641222 48-49 2.5154627592027156E-4 0.0 0.0 1.3349980106882011 0.001131958241641222 50-51 2.5154627592027156E-4 0.0 0.0 1.5826662815213686 0.0011738826209612672 52-53 2.5154627592027156E-4 0.0 0.0 1.8213627351800463 0.00119484481062129 54-55 2.5154627592027156E-4 0.0 0.0 2.0835997278269294 0.0012158070002813127 56-57 2.5154627592027156E-4 0.0 0.0 2.382541514568512 0.0012158070002813127 58-59 2.5154627592027156E-4 0.0 0.0 2.688484672656543 0.0012158070002813127 60-61 2.5154627592027156E-4 0.0 0.0 3.00084226078054 0.001257731379601358 62-63 2.5154627592027156E-4 0.0 0.0 3.2969751141076795 0.0012996557589214032 64-65 2.5154627592027156E-4 4.1924379320045264E-5 0.0 3.6054966215238924 0.001425428896881539 66-67 2.934706552403168E-4 4.1924379320045264E-5 0.0 3.937705403255931 0.001425428896881539 68-69 2.934706552403168E-4 4.1924379320045264E-5 0.0 4.313955745463677 0.001425428896881539 70-71 2.934706552403168E-4 4.1924379320045264E-5 0.0 4.739006065199956 0.001425428896881539 72-73 2.934706552403168E-4 4.1924379320045264E-5 0.0 5.167682843747419 0.0015302398451816522 74-75 2.934706552403168E-4 4.1924379320045264E-5 0.0 5.599797421399126 0.00159312641416172 76-77 2.934706552403168E-4 4.1924379320045264E-5 0.0 6.058764564005321 0.0016140886038217427 78-79 2.934706552403168E-4 4.1924379320045264E-5 0.0 6.578710716332522 0.0016350507934817652 80-81 2.934706552403168E-4 4.1924379320045264E-5 0.0 7.120960638457988 0.0016560129831417877 82-83 2.934706552403168E-4 4.1924379320045264E-5 0.0 7.669897499085 0.001760823931441901 84-85 2.934706552403168E-4 4.1924379320045264E-5 0.0 8.19776735910369 0.001760823931441901 86-87 3.144328449003395E-4 4.1924379320045264E-5 0.0 8.734944431331432 0.0017817861211019237 88-89 3.353950345603621E-4 4.1924379320045264E-5 0.0 9.307652415032909 0.0018446726900819914 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3935 0.0 55.489548 1 GTATCAA 7110 0.0 39.523026 1 ATCAACG 8760 0.0 31.626554 3 TCAACGC 8765 0.0 31.60851 4 TATCAAC 8905 0.0 31.333866 2 CAACGCA 8900 0.0 31.129053 5 AACGCAG 9280 0.0 30.007736 6 ACGCAGA 10665 0.0 25.84392 7 CGCAGAG 10825 0.0 25.505754 8 GTACATG 7450 0.0 23.255983 1 TACATGG 7460 0.0 22.772713 2 GCAGAGT 11885 0.0 22.592295 9 GAGTACT 7315 0.0 21.953136 12-13 ACATGGG 7535 0.0 21.909834 3 CAGAGTA 11360 0.0 20.817783 10-11 CATGGGG 4525 0.0 20.7582 4 GTACTTT 7795 0.0 20.449154 14-15 TGGTATC 1975 0.0 20.423134 2 GTGGTAT 2045 0.0 20.194078 1 AGAGTAC 10680 0.0 20.14437 10-11 >>END_MODULE