FastQCFastQC Report
Fri 27 May 2016
SRR939755_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939755_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1509074
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72830.48261384133581253No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54890.3637329912250824No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36780.243725622467818No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23080.15294147271770636No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA94400.029.1964281
GGTATCA65350.025.9148221
GTACATG76150.024.9181421
TATCAAC114400.024.8393822
TACATGG76050.024.8269772
TCAACGC117550.024.4191444
ATCAACG118300.024.1841163
CAACGCA117800.024.1659375
AACGCAG120500.023.8607036
CAACGCG1201.7244586E-423.7228975
ACATGGG78000.023.6620663
GAGTACT56800.021.92515212-13
ACGCAGA132750.021.301467
AGTACTT58050.021.2487212-13
CGCAGAG133400.021.1265358
CATGGGG33250.020.9760344
GTATAGG2751.2805685E-920.700181
AGAGTAC100050.020.6492310-11
GTACTTT61750.020.32360814-15
CATGGGA45100.019.8830474
Produced by FastQC (version 0.11.5)