##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939755_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1509074 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.99168562973055 31.0 30.0 33.0 16.0 34.0 2 29.813853396188655 31.0 30.0 33.0 25.0 34.0 3 30.0692020404566 31.0 30.0 34.0 25.0 34.0 4 33.85209207765822 35.0 35.0 37.0 28.0 37.0 5 33.82891362517677 35.0 35.0 37.0 28.0 37.0 6 33.88153596178849 35.0 35.0 37.0 29.0 37.0 7 33.800574391978124 35.0 35.0 37.0 28.0 37.0 8 33.67489400784852 35.0 33.0 37.0 28.0 37.0 9 34.953301826152995 37.0 34.0 39.0 28.0 39.0 10-11 34.77044498811854 37.0 34.0 39.0 27.0 39.0 12-13 34.63808501107302 37.0 34.0 39.0 27.0 39.0 14-15 35.27851318093082 38.0 33.5 40.0 27.0 41.0 16-17 35.11434329926829 38.0 33.0 40.0 27.0 41.0 18-19 34.99454963772486 38.0 33.5 40.0 26.0 41.0 20-21 35.216895261597514 38.0 34.0 40.0 26.5 41.0 22-23 35.12847448170203 38.0 34.0 40.0 25.5 41.0 24-25 34.91882041569863 38.0 33.5 40.0 25.0 41.0 26-27 34.79060536461433 38.0 33.5 40.0 25.0 41.0 28-29 34.817969496525684 38.0 34.0 40.0 25.0 41.0 30-31 34.59272408112524 38.0 33.0 40.0 24.0 41.0 32-33 34.24151565794653 38.0 33.0 40.0 23.5 41.0 34-35 34.02829616042686 38.0 33.0 40.0 23.0 41.0 36-37 33.796156450909635 38.0 33.0 40.0 21.5 41.0 38-39 33.569967741807226 38.0 32.5 40.0 20.0 41.0 40-41 33.33510914640369 37.0 32.0 40.0 19.0 41.0 42-43 32.92650956811926 37.0 31.0 40.0 16.0 41.0 44-45 32.530800013783285 37.0 31.0 40.0 14.5 41.0 46-47 32.10939224981678 37.0 30.0 39.5 10.5 41.0 48-49 31.70574802826104 36.0 30.0 39.0 8.5 40.5 50-51 31.08433781245983 35.5 29.5 38.5 8.0 40.0 52-53 31.258709314453764 36.0 30.0 39.0 7.0 40.0 54-55 31.349431174349302 36.0 30.0 39.0 4.0 40.0 56-57 31.04571313268932 36.0 29.0 39.0 2.0 40.0 58-59 30.646928513777322 35.5 28.5 39.0 2.0 40.0 60-61 30.223346900152016 35.0 28.0 39.0 2.0 40.0 62-63 29.78372763694822 35.0 28.0 38.0 2.0 40.0 64-65 29.231914737116934 34.0 26.5 38.0 2.0 40.0 66-67 28.62295321501795 34.0 26.0 37.5 2.0 39.5 68-69 28.04774550485927 33.5 25.5 37.0 2.0 39.0 70-71 27.46984309583228 33.0 24.5 36.0 2.0 39.0 72-73 26.804283288957336 32.0 22.5 36.0 2.0 38.5 74-75 26.434880926979062 32.0 22.0 35.0 2.0 38.0 76-77 25.870698521079813 32.0 20.5 35.0 2.0 37.5 78-79 25.17397622648061 31.0 18.0 35.0 2.0 37.0 80-81 24.413740147931776 31.0 12.5 34.0 2.0 36.0 82-83 23.62938729313473 30.0 7.0 34.0 2.0 35.5 84-85 22.79826933602991 29.5 2.0 34.0 2.0 35.0 86-87 21.784073544438513 29.0 2.0 34.0 2.0 35.0 88-89 20.705737094403588 28.0 2.0 33.5 2.0 35.0 90-91 19.214577946475785 25.5 2.0 32.5 2.0 34.5 92-93 17.626797625563757 21.5 2.0 32.0 2.0 34.0 94-95 17.109991955331548 20.0 2.0 31.0 2.0 34.0 96-97 16.80912268053124 19.0 2.0 31.5 2.0 34.0 98-99 15.927664581060968 6.0 2.0 31.5 2.0 34.0 100-101 14.303319121527506 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.5204635868676242 1101 2 0.7585704873155485 1101 3 0.9526262594011499 1101 4 0.659322953076142 1101 5 0.9358034407961071 1101 6 0.9583630934964233 1101 7 0.8881819265203674 1101 8 0.8395541572301326 1101 9 1.1682551658206393 1101 10-11 1.0221634871760088 1101 12-13 1.1346247945985652 1101 14-15 1.1332371182453116 1101 16-17 1.1006846625690514 1101 18-19 1.1360602430607258 1101 20-21 1.2542519639475955 1101 22-23 1.238578100892255 1101 24-25 1.2499541186859844 1101 26-27 1.3175824399761495 1101 28-29 1.1494340954069742 1101 30-31 1.203934462953157 1101 32-33 1.3548994638556664 1101 34-35 1.1634449320627809 1101 36-37 1.170843755928793 1101 38-39 1.2938233880040926 1101 40-41 1.2566943252100273 1101 42-43 1.1859365995084374 1101 44-45 0.7202824179082157 1101 46-47 0.6546154623250118 1101 48-49 0.7758930367006514 1101 50-51 0.9441484912164242 1101 52-53 0.7559903989428172 1101 54-55 0.6322190158632779 1101 56-57 0.6896401422482477 1101 58-59 0.5472940143441036 1101 60-61 0.4671693577731766 1101 62-63 -0.11126864272167936 1101 64-65 0.36688538275545923 1101 66-67 0.7255455382903513 1101 68-69 0.15628994713479827 1101 70-71 0.3764129259868412 1101 72-73 0.4232513519719312 1101 74-75 0.05879812613347468 1101 76-77 -0.1194122437610794 1101 78-79 -0.17352840399361824 1101 80-81 -0.21413198259984156 1101 82-83 -0.2242009064549606 1101 84-85 -0.44849353901780376 1101 86-87 -0.956081111220108 1101 88-89 -4.757937300998689 1101 90-91 -8.028215562285006 1101 92-93 -10.179635630723926 1101 94-95 -8.302959822252129 1101 96-97 -5.168885459263139 1101 98-99 -3.815769167555116 1101 100-101 -2.8946287052937443 1102 1 1.637838412183097 1102 2 0.7559718057285956 1102 3 0.7079826158373415 1102 4 0.6788529433319468 1102 5 0.6004140026647704 1102 6 0.6265647878063092 1102 7 0.8063716573241422 1102 8 0.9736889876777752 1102 9 0.9829928182686487 1102 10-11 1.0122043018040898 1102 12-13 0.8934242134149102 1102 14-15 1.1651120597281661 1102 16-17 1.2075468603171444 1102 18-19 1.1382396817815632 1102 20-21 1.1643113653561628 1102 22-23 1.0161463535615098 1102 24-25 1.0548650512499478 1102 26-27 1.1550705198160784 1102 28-29 0.8232517022202828 1102 30-31 1.0553168547527818 1102 32-33 1.2952057296119435 1102 34-35 1.222992330086889 1102 36-37 1.0312102206607179 1102 38-39 0.8532480300525194 1102 40-41 0.8454909352032018 1102 42-43 0.8740649870012049 1102 44-45 0.8987422118584973 1102 46-47 0.8675922253817134 1102 48-49 0.831967550271429 1102 50-51 0.7508806508546719 1102 52-53 0.8248949925823972 1102 54-55 0.6814826533933172 1102 56-57 0.9481028490521943 1102 58-59 0.7194686329856737 1102 60-61 0.587299950712282 1102 62-63 0.8243930085195785 1102 64-65 0.7931683387470976 1102 66-67 0.7757461437477886 1102 68-69 0.634193524743278 1102 70-71 0.40603491387075863 1102 72-73 0.657230136687776 1102 74-75 0.15272378594684 1102 76-77 0.31066470412311276 1102 78-79 0.15257631309546937 1102 80-81 0.012419372050498367 1102 82-83 -0.49018917875129375 1102 84-85 -0.39728522490121065 1102 86-87 -0.577653566721068 1102 88-89 -1.0158600895705945 1102 90-91 -6.13788450259578 1102 92-93 -10.533059100695525 1102 94-95 -8.487419054437776 1102 96-97 -5.051613624130656 1102 98-99 -3.478878222715549 1102 100-101 -2.4572196886245923 1103 1 2.0828708717114033 1103 2 1.3232670136023224 1103 3 0.9983543253724996 1103 4 0.8950791254550836 1103 5 0.835719349795248 1103 6 0.811561974857895 1103 7 0.6602239216949357 1103 8 0.8206515301617259 1103 9 0.9742004054331801 1103 10-11 0.9247964074276709 1103 12-13 0.7845654276393645 1103 14-15 0.8937699988799821 1103 16-17 0.9860923868863409 1103 18-19 0.7662593871699954 1103 20-21 0.7517327450283489 1103 22-23 0.917825062175865 1103 24-25 0.8352140254192832 1103 26-27 0.6018427932051651 1103 28-29 0.5505115954757329 1103 30-31 0.7100926511753869 1103 32-33 0.7190989344978362 1103 34-35 0.8065784073618474 1103 36-37 0.8901904794519808 1103 38-39 0.7488589876981635 1103 40-41 0.7097683867351492 1103 42-43 0.6336933828199136 1103 44-45 0.7938558992229829 1103 46-47 1.0129999554060873 1103 48-49 0.7026250216520467 1103 50-51 0.6192810208574784 1103 52-53 0.60840542577645 1103 54-55 0.6125875416023199 1103 56-57 0.5915240264449331 1103 58-59 0.4667097311101678 1103 60-61 0.5318275544805822 1103 62-63 0.5016848239985912 1103 64-65 0.30863934430767515 1103 66-67 0.3580441645699288 1103 68-69 0.3935216620235842 1103 70-71 0.43382536923126835 1103 72-73 0.6900029874160865 1103 74-75 0.40351446676464064 1103 76-77 0.42055321865457174 1103 78-79 0.1489831138986908 1103 80-81 0.05038132244567706 1103 82-83 -0.20904141151592626 1103 84-85 -0.08826103354486747 1103 86-87 0.03836022199384814 1103 88-89 -0.7477913735603892 1103 90-91 -6.447084424212498 1103 92-93 -9.758368683051701 1103 94-95 -8.28402939892387 1103 96-97 -5.065030439873054 1103 98-99 -3.539077004403362 1103 100-101 -2.8287893138387243 1104 1 1.7384555425269959 1104 2 1.0084610640109162 1104 3 0.8505625358758309 1104 4 0.5755478951393798 1104 5 0.5939909531782206 1104 6 0.5280167860146392 1104 7 0.7032847613902362 1104 8 0.5451081386258707 1104 9 0.7988789477664895 1104 10-11 0.7017302975903519 1104 12-13 0.6592116352367654 1104 14-15 0.8394565661068398 1104 16-17 0.7119615403881454 1104 18-19 0.9409829671711734 1104 20-21 0.8254522684899257 1104 22-23 0.713117313597337 1104 24-25 0.8061988490899878 1104 26-27 0.7926229841900749 1104 28-29 0.7328149414910072 1104 30-31 0.965258335202023 1104 32-33 0.6508028281039557 1104 34-35 0.7288233408866702 1104 36-37 0.6508095006753223 1104 38-39 0.7622789529278222 1104 40-41 0.47558931108866176 1104 42-43 0.5830527771105238 1104 44-45 0.40964837320821346 1104 46-47 0.6402450149103984 1104 48-49 0.3179429728895897 1104 50-51 0.4698934911473138 1104 52-53 0.49395988251089307 1104 54-55 0.3945170434794143 1104 56-57 0.51142154212938 1104 58-59 0.5388476870509358 1104 60-61 0.4938772827848119 1104 62-63 0.5795123286808312 1104 64-65 0.46284558953095356 1104 66-67 0.41979983574666235 1104 68-69 0.43888110250667367 1104 70-71 0.34990570635238427 1104 72-73 0.3623760000896219 1104 74-75 0.19743774105558742 1104 76-77 0.2094016191349759 1104 78-79 0.10373757987163046 1104 80-81 0.1420702454890055 1104 82-83 -0.09725403550073963 1104 84-85 -0.07042968648816839 1104 86-87 0.12972317310540404 1104 88-89 -0.17125935797252367 1104 90-91 -3.1384246990157596 1104 92-93 -8.033613398212074 1104 94-95 -5.273206185942943 1104 96-97 -2.862939915210468 1104 98-99 -2.2003900676366666 1104 100-101 -1.8049272235870184 1105 1 1.8342071290625839 1105 2 1.0852781419383568 1105 3 0.77450242546605 1105 4 0.6065422806967078 1105 5 0.6735188075899146 1105 6 0.6998783068680794 1105 7 0.5389715626099232 1105 8 0.5222827386822431 1105 9 0.7981102360419214 1105 10-11 0.6526770586092638 1105 12-13 0.5592634521011632 1105 14-15 0.5579189220089731 1105 16-17 0.5789731664412088 1105 18-19 0.5170194112246165 1105 20-21 0.5948631292477558 1105 22-23 0.5032307199298174 1105 24-25 0.5836333164154794 1105 26-27 0.45948930237409513 1105 28-29 0.5360683359226854 1105 30-31 0.5250991620511343 1105 32-33 0.7144057158976764 1105 34-35 0.8231170442835278 1105 36-37 0.8383598584109819 1105 38-39 0.615685271168779 1105 40-41 0.4038400363824124 1105 42-43 0.46071906796257167 1105 44-45 0.3380871837549435 1105 46-47 0.4891894242408483 1105 48-49 0.49423722007880855 1105 50-51 0.4920676214354103 1105 52-53 0.17290974400042813 1105 54-55 0.40065392541383815 1105 56-57 0.6167548000150838 1105 58-59 0.619622918589755 1105 60-61 0.6233237106953133 1105 62-63 0.5760664716099626 1105 64-65 0.5007313333449588 1105 66-67 0.32118711128995514 1105 68-69 0.30442861743249594 1105 70-71 0.19480227903692438 1105 72-73 0.1828659755193165 1105 74-75 0.057217782736845635 1105 76-77 0.2808044397375866 1105 78-79 -0.04392268940523891 1105 80-81 -0.10384597347425739 1105 82-83 -0.47866832983761043 1105 84-85 -0.4049797908336643 1105 86-87 -0.21487089728011455 1105 88-89 -0.3210230858827394 1105 90-91 -3.5832448987669245 1105 92-93 -8.169224289148962 1105 94-95 -5.835854234126932 1105 96-97 -3.320261289382323 1105 98-99 -2.3713531566620265 1105 100-101 -1.79503166161604 1106 1 1.5197616416454878 1106 2 0.9273895578230835 1106 3 0.6927746013355929 1106 4 0.8171797301145673 1106 5 0.7441605716074591 1106 6 0.851043883284369 1106 7 0.8225911195136106 1106 8 -0.4428916738188704 1106 9 -0.6142874316131497 1106 10-11 -0.7200645541665693 1106 12-13 -0.20185308323682705 1106 14-15 0.5229066377053115 1106 16-17 0.6662726692624261 1106 18-19 0.7543545037250965 1106 20-21 0.5012616959700438 1106 22-23 0.5606231635352685 1106 24-25 0.6079819637577017 1106 26-27 0.7777171786104589 1106 28-29 0.7085944494412573 1106 30-31 0.8742056515243917 1106 32-33 0.7215921008360979 1106 34-35 0.7585491752843083 1106 36-37 0.7253913715241751 1106 38-39 0.8217291331892653 1106 40-41 0.6535226902688436 1106 42-43 0.6615159999053404 1106 44-45 0.7017673680906356 1106 46-47 0.7759685986448943 1106 48-49 0.5225555298632187 1106 50-51 0.5418399128608513 1106 52-53 0.7661362454868872 1106 54-55 0.7447586325190478 1106 56-57 0.5938012834817421 1106 58-59 0.34825209489729403 1106 60-61 0.28800375159556424 1106 62-63 0.6093832951262996 1106 64-65 0.49774091583245195 1106 66-67 0.6306746545176658 1106 68-69 0.6169240749896403 1106 70-71 0.540155028325227 1106 72-73 0.7258102783442268 1106 74-75 0.6176569797598503 1106 76-77 0.3915068004235067 1106 78-79 0.2979964537660216 1106 80-81 0.16116850658379178 1106 82-83 0.03610129091984149 1106 84-85 0.10707090148269316 1106 86-87 0.2161885676710682 1106 88-89 0.036067912562543825 1106 90-91 -3.8692436382270152 1106 92-93 -8.916763679098406 1106 94-95 -6.32882320554098 1106 96-97 -3.699679572133192 1106 98-99 -2.6120532139208397 1106 100-101 -1.957998552639328 1107 1 1.8206505996622617 1107 2 1.1949966296057433 1107 3 0.7654597809816863 1107 4 0.8848733446920392 1107 5 0.7140037975399878 1107 6 0.7074857380918829 1107 7 0.5598818913397423 1107 8 0.5208788003319569 1107 9 0.5103768660940631 1107 10-11 0.5363292434668665 1107 12-13 0.6100708787481537 1107 14-15 0.7251782863654981 1107 16-17 0.7792405287839301 1107 18-19 0.9047541160771857 1107 20-21 0.7314725094324359 1107 22-23 0.6332574943042175 1107 24-25 0.6874372227280432 1107 26-27 0.8809766913386312 1107 28-29 0.7920418437852774 1107 30-31 0.6956784344136508 1107 32-33 0.8828949088905702 1107 34-35 0.882458516721556 1107 36-37 0.7584120341149543 1107 38-39 0.6151999379574917 1107 40-41 0.7365114476818064 1107 42-43 0.7237671893702498 1107 44-45 0.6376014691423038 1107 46-47 0.4789718729137675 1107 48-49 0.532006441753083 1107 50-51 0.5297725769830812 1107 52-53 0.5629086379365269 1107 54-55 0.16406704790850668 1107 56-57 0.41048589681092906 1107 58-59 0.3746923145644452 1107 60-61 0.14205471214082266 1107 62-63 0.20060697833978836 1107 64-65 0.2984886934374096 1107 66-67 0.2791330362072877 1107 68-69 0.3503712699262884 1107 70-71 0.531377714501911 1107 72-73 0.6306050283472189 1107 74-75 0.4282165965786646 1107 76-77 0.2296215234666228 1107 78-79 -0.10315851367075979 1107 80-81 -0.10446842046900784 1107 82-83 -0.17239465911664809 1107 84-85 -0.018425523143314138 1107 86-87 0.06734054399742462 1107 88-89 -0.12535294464981916 1107 90-91 -3.2120550028513506 1107 92-93 -4.644253180300833 1107 94-95 -3.081153081150667 1107 96-97 -1.794380919728141 1107 98-99 -1.0684308595580045 1107 100-101 -1.0608765990852902 1108 1 1.6991993618485104 1108 2 0.8246697917874108 1108 3 -0.6349606518235298 1108 4 -0.3735026619982236 1108 5 0.4834324900741578 1108 6 0.6872018600288499 1108 7 0.6874952764539017 1108 8 0.6378450396922517 1108 9 0.6829960364552647 1108 10-11 0.6073199527037829 1108 12-13 0.6195535287349756 1108 14-15 0.5418844760070343 1108 16-17 0.4495066501481091 1108 18-19 0.3649040742630234 1108 20-21 0.5247759427760741 1108 22-23 0.533099975201452 1108 24-25 0.3753151253755149 1108 26-27 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1.3004695808636164 2311 80-81 1.3312030691461878 2311 82-83 1.7294085050125254 2311 84-85 1.8121406291518767 2311 86-87 1.8192531123034357 2311 88-89 2.17026802429314 2311 90-91 2.8002297687745887 2311 92-93 3.7938252012047045 2311 94-95 3.5192541773295893 2311 96-97 2.821792568947668 2311 98-99 2.4924803454917726 2311 100-101 2.2964815478027063 2312 1 1.2501778308597657 2312 2 0.5695205694250767 2312 3 -0.00301912643937996 2312 4 -0.5937866281838282 2312 5 -0.1302473940975304 2312 6 -0.35728461888385965 2312 7 -0.006750671890408455 2312 8 0.22747186702400057 2312 9 0.1892311492477603 2312 10-11 0.22745679825046494 2312 12-13 0.1200322546235455 2312 14-15 0.3760935274292052 2312 16-17 -0.017204386235064817 2312 18-19 -0.45654284554455415 2312 20-21 0.4377645934696517 2312 22-23 0.4702117401820658 2312 24-25 0.611280283471821 2312 26-27 0.5395584642414661 2312 28-29 0.5403255685150228 2312 30-31 0.45667124096785017 2312 32-33 0.7146682082790861 2312 34-35 0.5816475687253302 2312 36-37 0.5872123862527658 2312 38-39 0.4679880921684756 2312 40-41 0.492525945607845 2312 42-43 0.3884931325682004 2312 44-45 0.13846277919013517 2312 46-47 0.26340563690212093 2312 48-49 0.38055545949480063 2312 50-51 0.0331250241029295 2312 52-53 0.10655786426437786 2312 54-55 0.35821791199441577 2312 56-57 0.2649748607802529 2312 58-59 0.2075672342627506 2312 60-61 0.20812060315258307 2312 62-63 0.4319369330535956 2312 64-65 0.5393890241614336 2312 66-67 0.7047332170348106 2312 68-69 0.7755538819322823 2312 70-71 0.8152979441845893 2312 72-73 0.48658304352721515 2312 74-75 0.4971364693649143 2312 76-77 0.6995105683324709 2312 78-79 0.5593201716814207 2312 80-81 0.7483367854602356 2312 82-83 1.0045474522826225 2312 84-85 1.3559382098347044 2312 86-87 1.9803502119251277 2312 88-89 2.2057287687702782 2312 90-91 2.749893493028477 2312 92-93 3.4480449947687646 2312 94-95 3.1015366489563228 2312 96-97 2.304313235894565 2312 98-99 2.122098298880216 2312 100-101 1.854559171454099 2313 1 -6.9513861072448115 2313 2 -2.4917607275288525 2313 3 -1.476534212868593 2313 4 -0.6825032121809329 2313 5 -0.5646536325777021 2313 6 -0.15910196325970105 2313 7 -0.041708182959439455 2313 8 0.009866728210297993 2313 9 -0.09621514245652918 2313 10-11 -0.1560188978571162 2313 12-13 -0.2300712189375247 2313 14-15 -0.03352963369608375 2313 16-17 -0.28965630981176105 2313 18-19 -0.6476746655837147 2313 20-21 -0.3516235291409231 2313 22-23 -1.131196747688577 2313 24-25 -0.021694637383696147 2313 26-27 -0.14238427985497992 2313 28-29 -0.3995222277939945 2313 30-31 0.12341300726313875 2313 32-33 -0.20453345401487866 2313 34-35 -0.07562964526103144 2313 36-37 0.18829562009741352 2313 38-39 0.3296555758018158 2313 40-41 0.2698828678211598 2313 42-43 0.20970877098967833 2313 44-45 0.06162786270065368 2313 46-47 -0.9776775649568847 2313 48-49 -0.438446372483007 2313 50-51 -0.7374879248056025 2313 52-53 -0.7199132155150174 2313 54-55 -0.5269598202393588 2313 56-57 -0.2679615573552887 2313 58-59 -0.2941068144242003 2313 60-61 0.06416101221699577 2313 62-63 -0.0859242508470679 2313 64-65 -0.043159528048335005 2313 66-67 0.0022662841942704404 2313 68-69 0.16226332673345567 2313 70-71 0.2614613060677762 2313 72-73 0.2039332967335774 2313 74-75 0.32913446866282925 2313 76-77 0.6242271555281356 2313 78-79 0.4245024778624078 2313 80-81 0.5042249428551919 2313 82-83 0.9799820772229495 2313 84-85 0.9938813009754028 2313 86-87 1.3829609202392348 2313 88-89 1.8211027889100393 2313 90-91 2.5831977060976534 2313 92-93 3.686597132569318 2313 94-95 3.322096261802077 2313 96-97 2.722007174854756 2313 98-99 2.135251795590891 2313 100-101 1.9754193294152067 2314 1 0.6132364970498756 2314 2 -0.05391184275770655 2314 3 -1.4065722555741331 2314 4 -0.28552924635228294 2314 5 -0.05586778005952908 2314 6 -3.2017197503023986 2314 7 -2.5407658697418682 2314 8 -2.3578430013477067 2314 9 -3.4748740157899682 2314 10-11 -2.5351673859158765 2314 12-13 -1.191078395031333 2314 14-15 -1.2187527195472043 2314 16-17 -2.184758517149362 2314 18-19 -2.677244779213993 2314 20-21 -2.662390707529866 2314 22-23 -4.210202127333005 2314 24-25 -3.0779132084850005 2314 26-27 -1.7932697842636287 2314 28-29 -1.9005421705759389 2314 30-31 -1.9404976411082657 2314 32-33 -2.1319514989227386 2314 34-35 -1.470667934777957 2314 36-37 -1.797803836031548 2314 38-39 -3.4507656152128945 2314 40-41 -2.5560047777290045 2314 42-43 -1.4255649639332049 2314 44-45 -1.9745333806549539 2314 46-47 -1.5453562004991568 2314 48-49 -2.218244628433588 2314 50-51 -3.0970442296552108 2314 52-53 -1.974881191210212 2314 54-55 -2.7212748469181918 2314 56-57 -1.630499428136659 2314 58-59 -1.0203929865314763 2314 60-61 -1.5499938950473187 2314 62-63 -1.1413142107286163 2314 64-65 -1.654287515151701 2314 66-67 -0.7586806446071854 2314 68-69 -1.0138091742168704 2314 70-71 -1.1625043327243922 2314 72-73 -2.0434537722905013 2314 74-75 -1.8283200157313075 2314 76-77 -1.1770268150764167 2314 78-79 -1.9410437494884292 2314 80-81 -0.7406514004210543 2314 82-83 -0.455523997923315 2314 84-85 -1.2379104526553633 2314 86-87 0.1219646291604306 2314 88-89 1.0598295206083392 2314 90-91 2.2082007121337703 2314 92-93 3.1364332455895223 2314 94-95 3.0637062567772517 2314 96-97 2.3256318848513793 2314 98-99 1.952709250159666 2314 100-101 1.7207532230314833 2315 1 -2.743297954232105 2315 2 -1.853572498378412 2315 3 -2.585594603036075 2315 4 -3.93855813666854 2315 5 -3.777941970778439 2315 6 -2.9171230843494165 2315 7 -5.689337929774052 2315 8 -2.7062479216109843 2315 9 -2.3094986534811355 2315 10-11 -3.6471303112762286 2315 12-13 -2.874043721586986 2315 14-15 -2.1307943801705918 2315 16-17 -2.591336825354169 2315 18-19 -2.141433126855503 2315 20-21 -1.2277851781042912 2315 22-23 -0.6556926917635479 2315 24-25 -2.2047446666758788 2315 26-27 -3.798945399584312 2315 28-29 -2.974686845470675 2315 30-31 -2.9293188452973453 2315 32-33 -4.146533177549337 2315 34-35 -3.5609660006991746 2315 36-37 -2.687138661190918 2315 38-39 -1.6786250060045518 2315 40-41 -1.3732369799911481 2315 42-43 -2.9808997384441653 2315 44-45 -6.268440836792273 2315 46-47 -3.092268143625592 2315 48-49 -1.953146220865797 2315 50-51 -3.09791561261018 2315 52-53 -3.132936936697323 2315 54-55 -1.660349178071339 2315 56-57 -1.2944748222158609 2315 58-59 -2.611251408096649 2315 60-61 -2.9348184788669904 2315 62-63 -4.619470930737272 2315 64-65 -4.007765795039447 2315 66-67 -3.5233282812830815 2315 68-69 -2.1059876776483044 2315 70-71 -3.5536334930887854 2315 72-73 -2.6719444265068653 2315 74-75 -1.7597586379102488 2315 76-77 -3.202665042658161 2315 78-79 -2.1208534495807463 2315 80-81 -1.952545720201332 2315 82-83 -1.6408414829072129 2315 84-85 -2.666621427476194 2315 86-87 -2.3853699519656644 2315 88-89 -1.5393907240235016 2315 90-91 -0.8137339652243156 2315 92-93 -0.03390520093150684 2315 94-95 0.1150678933656657 2315 96-97 -0.2786032435078205 2315 98-99 -0.7780345678559755 2315 100-101 -1.1997582338701704 2316 1 -7.224270307522797 2316 2 -5.87733858235211 2316 3 -3.109132503044563 2316 4 -3.871180454757983 2316 5 -1.6901101178946831 2316 6 -2.4858873458472424 2316 7 -3.2371177547165075 2316 8 -2.331583831375962 2316 9 -7.021508020507806 2316 10-11 -6.946323428808448 2316 12-13 -6.131822448050833 2316 14-15 -4.676026483707197 2316 16-17 -4.641930752764708 2316 18-19 -4.100199735103082 2316 20-21 -2.559550932534407 2316 22-23 -3.229021631182448 2316 24-25 -3.101545597386849 2316 26-27 -4.034575052515002 2316 28-29 -4.067859051964682 2316 30-31 -6.531836608195732 2316 32-33 -4.8430682265065315 2316 34-35 -5.41255904433374 2316 36-37 -4.256129608728578 2316 38-39 -6.471652413045799 2316 40-41 -3.65491086166503 2316 42-43 -4.694686893234778 2316 44-45 -3.1591749200869117 2316 46-47 -4.263301262755597 2316 48-49 -5.501012426171446 2316 50-51 -4.961088867651945 2316 52-53 -6.09032994737753 2316 54-55 -5.283561528965354 2316 56-57 -4.310153107790338 2316 58-59 -3.1112955968951646 2316 60-61 -3.3342759802276056 2316 62-63 -4.107217901183205 2316 64-65 -3.068059538205162 2316 66-67 -4.031403596970858 2316 68-69 -3.0429759488926287 2316 70-71 -2.64598758267471 2316 72-73 -2.2120084378511535 2316 74-75 -2.209119023775827 2316 76-77 -2.7675319519887402 2316 78-79 -4.475993330845196 2316 80-81 -5.639099093778924 2316 82-83 -3.0427845417706862 2316 84-85 -1.63402082930466 2316 86-87 -1.718950942294061 2316 88-89 -1.1160632825299714 2316 90-91 -0.9705932151631522 2316 92-93 -1.2311000860156618 2316 94-95 -1.7564478073887884 2316 96-97 -3.529968602485635 2316 98-99 -4.933047375117917 2316 100-101 -4.059117770911367 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11731.0 3 3936.0 4 3787.0 5 4151.0 6 4673.0 7 5421.0 8 6699.0 9 8005.0 10 9408.0 11 11543.0 12 13523.0 13 15597.0 14 17175.0 15 19391.0 16 21892.0 17 23407.0 18 21470.0 19 20498.0 20 22094.0 21 24230.0 22 26853.0 23 30377.0 24 34200.0 25 38231.0 26 43478.0 27 49628.0 28 57044.0 29 64939.0 30 73812.0 31 83981.0 32 93136.0 33 104165.0 34 117668.0 35 135347.0 36 144045.0 37 111696.0 38 31348.0 39 495.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.181480636120426 19.98837530256062 13.757617096267358 22.072526965051594 2 15.548511477880814 20.88482048437838 40.06424588047506 23.50242215726574 3 19.390735911033325 25.60717752267192 30.048677700809613 24.95340886548514 4 12.573328157813544 17.003042276697887 37.6892631187815 32.73436644670708 5 13.335323680151268 38.01967126105057 33.96327810120914 14.681726957589024 6 30.670699258288025 36.639496660371826 17.949987193557764 14.73981688778239 7 27.710198834088988 33.7058195326931 20.99642049523673 17.58756113798118 8 26.42337364989299 34.427554626449655 20.09901614321504 19.050055580442322 9 27.142168777054625 16.751865367026973 20.47762156909856 35.62834428681984 10-11 24.938609671664615 26.95785371447989 27.225382810177123 20.878153803678376 12-13 26.109480290966186 24.56340306872903 27.704365985902946 21.622750654401838 14-15 22.917937951669103 25.547809772958868 26.697499372030837 24.836752903341193 16-17 21.813051558357145 29.084986729585143 27.057113383658827 22.044848328398885 18-19 21.688472227983574 27.357995602936906 29.872319243373298 21.08121292570623 20-21 22.794078603269337 26.266988538461334 29.827977358350307 21.110955499919022 22-23 22.284674719353838 26.3802579139479 29.71239059239869 21.622676774299567 24-25 22.135266293998406 26.50929816726242 29.925538290916997 21.429897247822183 26-27 21.764809415327804 26.78397082948666 30.0003320869005 21.450887668285038 28-29 21.40253923366057 27.255534919028847 30.012044307910557 21.32988153940003 30-31 21.684628694551225 26.711904612484066 29.939956123442744 21.663510569521964 32-33 21.601003312498506 26.827817815238557 29.74036599522384 21.8308128770391 34-35 22.190345155479 26.799633987379472 29.572352235353023 21.437668621788507 36-37 22.069947902658658 26.473551219091068 29.78194822141868 21.67455265683159 38-39 21.715675370594777 26.934114639531217 29.763018542188874 21.587191447685132 40-41 21.837654709976274 26.732828097370252 29.77221219910449 21.65730499354898 42-43 21.746477563974874 26.783461482278266 29.37173817776184 22.09832277598502 44-45 21.74038469837674 26.846126624736833 29.40736319412124 22.00612548276519 46-47 21.96235430987771 27.20670068316316 28.893890577023196 21.937054429935934 48-49 21.86850050318685 27.127151829574103 28.857616388945168 22.14673127829388 50-51 21.69654995119187 27.377988812824896 28.848875486628167 22.076585749355065 52-53 21.970041954018978 27.570210952553197 28.424171706230855 22.035575387196964 54-55 21.435415915888488 27.269481344442358 28.71911291648468 22.57598982318447 56-57 21.716494011786157 27.47913912639519 28.58676028117559 22.21760658064306 58-59 21.967368258516977 27.01205551414003 28.539640742025945 22.480935485317044 60-61 21.69782038501209 27.09362647606281 29.049427335267392 22.159125803657712 62-63 21.493931383002028 27.155318355093428 28.92783783030179 22.422912431602747 64-65 21.310836545761205 27.20540517752751 28.73774406070136 22.746014216009925 66-67 21.401470825984347 27.387486260594407 28.40670034833605 22.804342565085197 68-69 21.37051195015113 27.19980214486913 28.65898373504675 22.770702169932992 70-71 21.480708068018085 27.40742462169753 28.369066510852747 22.742800799431635 72-73 21.614787775417735 27.206397090371954 28.375282773498263 22.80353236071205 74-75 21.349984806444063 27.707367131107937 28.083868428787056 22.85877963366095 76-77 21.640212624337543 27.323768515283202 28.080920539558836 22.95509832082042 78-79 21.700875869401102 27.521350375327525 28.03625702753088 22.741516727740493 80-81 21.764809248370582 27.736375464924812 27.81918747236693 22.67962781433767 82-83 21.813876841116784 27.619151700283247 27.792812349172173 22.7741591094278 84-85 21.71026129545505 27.453341520575425 27.80091186571152 23.035485318258004 86-87 21.66286018115041 27.356813431951032 28.043907038284154 22.936419348614404 88-89 21.721098050885285 27.402716963887176 27.730049206113033 23.146135779114502 90-91 21.714738683286434 27.350199804588183 27.94149359587026 22.993567916255124 92-93 22.237375716902502 26.873811828258027 27.992193061743603 22.89661939309587 94-95 21.822413455506627 26.974010306252133 27.918733463272382 23.28484277496886 96-97 22.09282315216799 27.007861616415312 27.545324557451856 23.35399067396484 98-99 22.18543420494681 27.067905296278127 27.06910165456351 23.677558844211553 100-101 22.277689769810603 27.048301416688215 27.31809772496266 23.355911088538527 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2393.0 1 1508.0 2 655.5 3 717.0 4 663.5 5 642.5 6 876.5 7 1596.5 8 2532.5 9 2944.5 10 2640.0 11 2168.0 12 2032.0 13 2087.0 14 2178.5 15 2349.5 16 2504.0 17 2610.0 18 2844.5 19 3079.0 20 3366.0 21 3837.0 22 4478.0 23 5329.5 24 6361.5 25 7754.0 26 9698.5 27 11819.0 28 13849.5 29 16020.5 30 18637.5 31 21250.5 32 23921.0 33 26539.5 34 29133.5 35 31790.0 36 34334.0 37 37474.0 38 39995.5 39 41068.5 40 42685.5 41 45927.5 42 49019.0 43 51777.5 44 53997.5 45 55290.0 46 56343.0 47 58361.0 48 62158.0 49 61975.0 50 58009.0 51 54764.5 52 52560.0 53 51164.5 54 48556.0 55 44276.5 56 39441.0 57 34990.0 58 31079.5 59 27216.0 60 23112.0 61 18945.0 62 14923.0 63 11341.5 64 8533.5 65 6612.0 66 5166.5 67 3975.0 68 2980.5 69 2187.0 70 1668.5 71 1301.0 72 1045.0 73 869.5 74 702.5 75 554.0 76 385.0 77 239.0 78 141.5 79 92.5 80 61.5 81 43.5 82 33.5 83 23.0 84 18.0 85 10.5 86 4.5 87 5.0 88 5.0 89 3.5 90 4.5 91 4.5 92 2.5 93 2.5 94 1.5 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12842312570490247 2 0.11530249676291553 3 0.120139900362739 4 0.1311400236171321 5 0.1192121791244167 6 0.1338569215293617 7 0.16175482448176828 8 0.1490317903562052 9 0.15903792656953866 10-11 0.15386919395602866 12-13 0.14336606422216538 14-15 0.1479384046110396 16-17 0.14250460878658036 18-19 0.15814333823258503 20-21 0.16367653276115024 22-23 0.1955835167791639 24-25 0.21645724464141586 26-27 0.228219424627288 28-29 0.22421034356168087 30-31 0.21592711821951738 32-33 0.21615904852909798 34-35 0.20641797552671373 36-37 0.2146349350661399 38-39 0.20204443254605142 40-41 0.2061529123157645 42-43 0.19972512945024565 44-45 0.19263468855735372 46-47 0.2079420889896718 48-49 0.24312923024318225 50-51 0.24448767919929706 52-53 0.24753590612521323 54-55 0.24462021080477167 56-57 0.22030066120017974 58-59 0.22076452181934084 60-61 0.19306541627514623 62-63 0.1855442476644618 64-65 0.18650510180415275 66-67 0.19306541627514623 68-69 0.19359554269704468 70-71 0.19906247142287256 72-73 0.22874955104918646 74-75 0.2320297082846832 76-77 0.24491840691708958 78-79 0.2422346419062286 80-81 0.2556203340591648 82-83 0.2544275496098932 84-85 0.23057186062446244 86-87 0.23388515076132782 88-89 0.23656891577218878 90-91 0.43775189288265515 92-93 0.58777767027992 94-95 0.3336814496837133 96-97 0.2677138430587234 98-99 0.2987262387397835 100-101 0.31747946091444157 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1509074.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.4199816144698 #Duplication Level Percentage of deduplicated Percentage of total 1 80.81702358336148 48.82963079042872 2 11.042663989313171 13.34395110418139 3 3.4838084543996755 6.314749282894885 4 1.5466104240679108 3.7378469354772204 5 0.8350470058889132 2.522676237151309 6 0.5068848150130387 1.8375582722245036 7 0.3210486936623354 1.3578429317899496 8 0.21875743390417307 1.0573856107614983 9 0.1710985312732813 0.9303993102414998 >10 0.937332595233553 10.469334856594532 >50 0.08012844141204203 3.3362872984042897 >100 0.037128360865076156 4.050827081589881 >500 0.0015703364761302189 0.6196322188515754 >1k 6.73001346912951E-4 0.7263041112799228 >5k 2.2433378230431698E-4 0.8655739581288769 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7283 0.48261384133581253 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5489 0.3637329912250824 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3678 0.243725622467818 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2308 0.15294147271770636 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 6.626580273730778E-5 0.0 0.0 0.0 0.0 6 1.3253160547461556E-4 0.0 0.0 0.0 0.0 7 1.3253160547461556E-4 0.0 0.0 0.0 0.0 8 1.3253160547461556E-4 0.0 0.0 0.0 0.0 9 1.3253160547461556E-4 0.0 0.0 0.0 0.0 10-11 1.9879740821192334E-4 0.0 0.0 1.3253160547461556E-4 0.0 12-13 3.313290136865389E-4 0.0 0.0 1.9879740821192334E-4 0.0 14-15 3.975948164238467E-4 0.0 0.0 1.9879740821192334E-4 0.0 16-17 4.3072771779250057E-4 0.0 0.0 1.9879740821192334E-4 0.0 18-19 4.6386061916115444E-4 0.0 0.0 1.9879740821192334E-4 0.0 20-21 4.6386061916115444E-4 0.0 0.0 2.3193030958057725E-4 0.0 22-23 4.6386061916115444E-4 0.0 0.0 4.3072771779250057E-4 0.0 24-25 5.301264218984623E-4 0.0 0.0 0.001225917350640194 0.0 26-27 5.301264218984623E-4 0.0 0.0 0.002816296616335581 0.0 28-29 5.9639222463577E-4 0.0 0.0 0.009177813679117127 0.0 30-31 5.9639222463577E-4 0.0 0.0 0.029985275738631773 0.0 32-33 5.9639222463577E-4 0.0 0.0 0.0766695337670651 0.0 34-35 6.957909287417317E-4 0.0 0.0 0.14535403830428462 0.0 36-37 7.951896328476934E-4 0.0 0.0 0.2596625480261405 0.0 38-39 7.951896328476934E-4 0.0 0.0 0.43158917322808554 0.0 40-41 8.614554355850011E-4 0.0 0.0 0.6150791810076908 0.0 42-43 9.277212383223089E-4 0.0 0.0 0.8025120040501659 0.0 44-45 9.277212383223089E-4 0.0 0.0 1.0320567447322 0.0 46-47 9.939870410596166E-4 0.0 0.0 1.2992073284676562 0.0 48-49 0.0010602528437969245 3.313290136865389E-5 0.0 1.5929304991007731 0.0 50-51 0.0010602528437969245 6.626580273730778E-5 0.0 1.8959640150184816 0.0 52-53 0.0011265186465342321 6.626580273730778E-5 0.0 2.1847503833476685 0.0 54-55 0.00119278444927154 6.626580273730778E-5 0.0 2.49361528990626 0.0 56-57 0.00119278444927154 6.626580273730778E-5 0.0 2.855923566372491 0.0 58-59 0.00119278444927154 6.626580273730778E-5 0.0 3.247355663141768 0.0 60-61 0.00119278444927154 6.626580273730778E-5 0.0 3.6605560761102502 0.0 62-63 0.00119278444927154 6.626580273730778E-5 0.0 4.059078613772419 0.0 64-65 0.00119278444927154 6.626580273730778E-5 0.0 4.45289627944024 0.0 66-67 0.0013253160547461555 6.626580273730778E-5 0.0 4.882961339205367 0.0 68-69 0.0013915818574834634 6.626580273730778E-5 0.0 5.365442648935705 0.0 70-71 0.0013915818574834634 6.626580273730778E-5 0.0 5.884635213382511 0.0 72-73 0.0013915818574834634 6.626580273730778E-5 0.0 6.38480949244371 0.0 74-75 0.0013915818574834634 6.626580273730778E-5 0.0 6.877793931907911 0.0 76-77 0.0013915818574834634 6.626580273730778E-5 0.0 7.383998399018205 0.0 78-79 0.0014578476602207712 6.626580273730778E-5 0.0 7.947191456482585 0.0 80-81 0.001490980561589425 6.626580273730778E-5 0.0 8.51883340379597 0.0 82-83 0.0015241134629580789 6.626580273730778E-5 0.0 9.049920679834123 0.0 84-85 0.0015241134629580789 6.626580273730778E-5 0.0 9.542308727073689 0.0 86-87 0.0015241134629580789 6.626580273730778E-5 0.0 10.045763163370385 0.0 88-89 0.0015241134629580789 6.626580273730778E-5 0.0 10.578573350279708 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9440 0.0 29.196428 1 GGTATCA 6535 0.0 25.914822 1 GTACATG 7615 0.0 24.918142 1 TATCAAC 11440 0.0 24.839382 2 TACATGG 7605 0.0 24.826977 2 TCAACGC 11755 0.0 24.419144 4 ATCAACG 11830 0.0 24.184116 3 CAACGCA 11780 0.0 24.165937 5 AACGCAG 12050 0.0 23.860703 6 CAACGCG 120 1.7244586E-4 23.722897 5 ACATGGG 7800 0.0 23.662066 3 GAGTACT 5680 0.0 21.925152 12-13 ACGCAGA 13275 0.0 21.30146 7 AGTACTT 5805 0.0 21.24872 12-13 CGCAGAG 13340 0.0 21.126535 8 CATGGGG 3325 0.0 20.976034 4 GTATAGG 275 1.2805685E-9 20.70018 1 AGAGTAC 10005 0.0 20.64923 10-11 GTACTTT 6175 0.0 20.323608 14-15 CATGGGA 4510 0.0 19.883047 4 >>END_MODULE