FastQCFastQC Report
Fri 27 May 2016
SRR939755_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939755_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1509074
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76560.5073309857568283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65300.4327156918746198No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47720.3162204106624327No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26050.17262241613068677No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15660.103772247086624No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66700.032.469111
GTATCAA97450.027.6463471
TACATGG73900.026.1081432
GTACATG77300.025.2089121
ACATGGG76250.024.0035023
TCAACGC117850.022.774614
TATCAAC118300.022.6894862
CAACGCA118550.022.6401355
ATCAACG118750.022.6027553
AACGCAG119850.022.4175666
GAGTACT64600.021.9262112-13
CATGGGG31100.021.2342014
GGACCGA2701.1023076E-920.9492826
GTACTTT68150.020.54197514-15
CTCGCGA600.00413588820.28205964-65
CGCAGAG134950.019.8044038
AGTACTT66100.019.78851112-13
ACGCAGA135500.019.7240187
CATGGGA46650.019.7174744
GTAGGAC4100.019.559053
Produced by FastQC (version 0.11.5)