##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939755_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1509074 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.33765209658373 31.0 31.0 34.0 30.0 34.0 2 31.54845819356771 31.0 31.0 34.0 30.0 34.0 3 31.595707036235467 31.0 31.0 34.0 30.0 34.0 4 35.4157476704257 37.0 35.0 37.0 33.0 37.0 5 35.14620224057932 37.0 35.0 37.0 32.0 37.0 6 35.180846664908415 37.0 35.0 37.0 32.0 37.0 7 35.1007697435646 37.0 35.0 37.0 32.0 37.0 8 35.046239614492066 37.0 35.0 37.0 32.0 37.0 9 36.627986434064866 39.0 35.0 39.0 32.0 39.0 10-11 36.481337561975096 38.0 35.0 39.0 32.0 39.0 12-13 36.4143700043868 38.0 35.0 39.0 32.0 39.0 14-15 37.40175398953265 39.0 36.0 41.0 32.0 41.0 16-17 37.3318896223777 39.0 36.0 41.0 32.0 41.0 18-19 37.33247706871896 39.0 36.0 40.5 32.0 41.0 20-21 37.26567385032145 39.0 36.0 40.0 31.0 41.0 22-23 37.11518620027911 39.0 36.0 40.0 31.0 41.0 24-25 36.941042983975606 39.0 36.0 40.0 31.0 41.0 26-27 36.62888400436294 38.5 35.0 40.0 30.0 41.0 28-29 36.38461699028676 38.0 35.0 40.0 30.0 41.0 30-31 36.10399523151284 38.0 35.0 40.0 29.5 41.0 32-33 36.02924409273501 38.0 35.0 40.0 30.0 41.0 34-35 36.08581023859665 38.0 35.0 40.0 30.0 41.0 36-37 36.031321525650824 38.0 35.0 40.0 30.0 41.0 38-39 35.88305146069709 38.0 35.0 40.0 29.0 41.0 40-41 35.70004154865832 38.0 34.5 40.0 28.5 41.0 42-43 35.46967743132544 38.0 34.0 40.0 27.5 41.0 44-45 35.17922282141234 38.0 34.0 40.0 26.5 41.0 46-47 34.819801414642356 38.0 33.5 40.0 25.5 41.0 48-49 34.465142531115106 38.0 33.0 40.0 24.0 41.0 50-51 34.172439853844146 38.0 33.0 40.0 24.0 41.0 52-53 33.883751227573995 37.5 33.0 40.0 23.0 41.0 54-55 33.55947687124687 37.0 32.5 40.0 22.5 41.0 56-57 32.13770563935234 36.5 30.5 39.5 14.0 40.5 58-59 28.501036397154813 34.0 22.0 38.0 6.5 40.0 60-61 28.513428102266687 33.5 22.0 38.0 6.5 40.0 62-63 30.495496907374985 34.5 28.5 38.0 2.0 40.0 64-65 30.012285017169468 35.0 28.0 38.0 2.0 40.0 66-67 24.392962836812508 28.0 16.5 35.5 2.0 39.0 68-69 16.741796624950133 13.0 7.0 28.5 2.0 35.0 70-71 15.636293515096012 12.0 7.0 25.0 2.0 32.5 72-73 17.552939087148808 15.5 7.0 29.0 2.0 34.0 74-75 18.258667235669026 17.5 7.5 30.0 2.0 34.5 76-77 19.338224964448397 21.5 7.5 30.5 2.0 34.5 78-79 22.35531491497435 27.5 9.5 33.5 2.0 35.5 80-81 24.991215142531118 30.5 16.5 34.5 2.0 36.0 82-83 26.040037135355853 32.0 23.5 35.0 2.0 36.0 84-85 26.054540400271954 33.0 23.5 35.0 2.0 36.0 86-87 25.762619328144282 32.5 22.0 35.0 2.0 35.5 88-89 25.28963125731409 32.0 19.0 35.0 2.0 35.0 90-91 23.53504698908072 31.5 4.5 34.0 2.0 35.0 92-93 21.25421251708001 29.5 2.0 34.0 2.0 35.0 94-95 16.818693119091577 18.0 2.0 32.0 2.0 35.0 96-97 15.45901493233599 6.5 2.0 31.5 2.0 34.0 98-99 14.345970774130361 2.0 2.0 31.0 2.0 34.0 100-101 13.724983334150611 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.42526614729588275 1101 2 0.31516147446562215 1101 3 0.2879358662573104 1101 4 0.27807881972737647 1101 5 0.2908861769540678 1101 6 0.4398561502001286 1101 7 0.430394441248346 1101 8 0.26243195265226404 1101 9 0.4528395057182806 1101 10-11 0.4727251936315966 1101 12-13 0.44716031630586883 1101 14-15 0.42498277374626525 1101 16-17 0.24321787578129062 1101 18-19 0.5282858765389378 1101 20-21 0.5097213880525331 1101 22-23 0.41250417976598897 1101 24-25 0.40858657432861634 1101 26-27 0.47504171218993463 1101 28-29 0.5932674977808077 1101 30-31 0.5672522612787176 1101 32-33 0.5205699125731229 1101 34-35 0.5019391752213878 1101 36-37 0.7254145774407519 1101 38-39 0.5971474906068153 1101 40-41 0.7442720751735195 1101 42-43 0.7970888752500116 1101 44-45 0.6930118455957341 1101 46-47 0.4353694078345782 1101 48-49 0.2581031305366821 1101 50-51 0.3540227742081683 1101 52-53 0.6078100284129064 1101 54-55 0.5246430976072247 1101 56-57 1.166785700805793 1101 58-59 -7.836554667727324 1101 60-61 -9.405546278352627 1101 62-63 -2.7324713399994565 1101 64-65 -0.15021536397749813 1101 66-67 -0.936575008953838 1101 68-69 -2.955483245789795 1101 70-71 -3.919900443087462 1101 72-73 -6.484046908461622 1101 74-75 -7.145565841872697 1101 76-77 -8.162020547838406 1101 78-79 -11.368705009015049 1101 80-81 -7.6062795751699 1101 82-83 -3.643324252836752 1101 84-85 -2.4292847532230475 1101 86-87 -2.332859953238117 1101 88-89 -2.5037202597803514 1101 90-91 -7.960825730228526 1101 92-93 -13.811449973609463 1101 94-95 -12.347016311688671 1101 96-97 -12.044965445590504 1101 98-99 -11.471214291652384 1101 100-101 -11.165918855311663 1102 1 0.380262558244052 1102 2 0.35545664363653273 1102 3 0.3133532885898802 1102 4 0.266749206061931 1102 5 0.37259657688193926 1102 6 0.36859942722985295 1102 7 0.34003910777861535 1102 8 0.4542447532570222 1102 9 0.5210127650281393 1102 10-11 0.434416660010676 1102 12-13 0.4238696239136175 1102 14-15 0.611322877152567 1102 16-17 0.6011805427068779 1102 18-19 0.6662046425986432 1102 20-21 0.6148192287263683 1102 22-23 0.6534183303397114 1102 24-25 0.547424623132784 1102 26-27 0.6499689775405031 1102 28-29 0.5759259622214472 1102 30-31 0.666051055912142 1102 32-33 0.2633181206479662 1102 34-35 0.2885490643595645 1102 36-37 0.44317119464670185 1102 38-39 0.33593456447192693 1102 40-41 0.4576673875597308 1102 42-43 0.49470588354124345 1102 44-45 0.33756914385460846 1102 46-47 0.4550318635782773 1102 48-49 0.4363187996087632 1102 50-51 0.40983825186415146 1102 52-53 0.4186267717731553 1102 54-55 0.1633337698869184 1102 56-57 0.6546453428715502 1102 58-59 -8.751202853955764 1102 60-61 -9.517123484579198 1102 62-63 -3.108898031448497 1102 64-65 -0.45827217849460666 1102 66-67 -1.542683332433171 1102 68-69 -3.633785606935101 1102 70-71 -4.213729354547114 1102 72-73 -6.905852739023699 1102 74-75 -7.628232975520666 1102 76-77 -8.640385327276432 1102 78-79 -11.664649553829648 1102 80-81 -8.174229168930847 1102 82-83 -4.303256216897687 1102 84-85 -3.3006243538153264 1102 86-87 -3.119172315180986 1102 88-89 -3.281243085803233 1102 90-91 -8.317980080263375 1102 92-93 -14.163068167848255 1102 94-95 -12.0189440313057 1102 96-97 -11.648603391751259 1102 98-99 -11.34353559328185 1102 100-101 -10.94584374529942 1103 1 0.3253078709448829 1103 2 0.31024592535489504 1103 3 0.2893444051513008 1103 4 0.16596684820010665 1103 5 0.13538980304931414 1103 6 0.17044017010108092 1103 7 0.2850785206625517 1103 8 0.33654087770944585 1103 9 0.177898799529423 1103 10-11 0.3246840692114503 1103 12-13 0.4164232121611846 1103 14-15 0.4874465288863803 1103 16-17 0.5194963135495598 1103 18-19 0.5958518546190916 1103 20-21 0.5573615441095185 1103 22-23 0.5985641506355037 1103 24-25 0.44621832128550665 1103 26-27 0.19429978027380201 1103 28-29 0.3892488023090195 1103 30-31 0.4565561434506762 1103 32-33 0.5121818791263166 1103 34-35 0.5596433328981547 1103 36-37 0.14643252914314786 1103 38-39 0.2624837404992917 1103 40-41 0.38607015963084024 1103 42-43 0.6138661710308924 1103 44-45 0.5536741690236511 1103 46-47 0.30630464371949273 1103 48-49 0.35813484753212776 1103 50-51 0.5392819202017307 1103 52-53 0.520855427605234 1103 54-55 0.3604169425556023 1103 56-57 0.9865132038297268 1103 58-59 -6.589475176861175 1103 60-61 -8.811937545528522 1103 62-63 -2.453843679385354 1103 64-65 -0.0933579681534944 1103 66-67 -1.8669886816707262 1103 68-69 -4.059343824431851 1103 70-71 -4.472360470602833 1103 72-73 -7.368592656131272 1103 74-75 -7.91467898903546 1103 76-77 -8.814648640318609 1103 78-79 -11.848302846875661 1103 80-81 -8.05089391815702 1103 82-83 -4.204401413498548 1103 84-85 -3.3305471715678756 1103 86-87 -3.0539315078331875 1103 88-89 -3.186642471800859 1103 90-91 -8.645725865581682 1103 92-93 -14.19663327970246 1103 94-95 -12.024553071510846 1103 96-97 -11.733243850588448 1103 98-99 -11.410689837673335 1103 100-101 -11.168958595770668 1104 1 0.09549713413283456 1104 2 0.05779037511847562 1104 3 0.10407476704838459 1104 4 0.2202388127242827 1104 5 0.0658554232463473 1104 6 0.11092220962504484 1104 7 0.11935984966218882 1104 8 0.02964539029479596 1104 9 0.006777708043117059 1104 10-11 0.14667714873809956 1104 12-13 0.20027600092716824 1104 14-15 0.2182378060664547 1104 16-17 0.4393150920524178 1104 18-19 0.27689908013078224 1104 20-21 0.285335080017866 1104 22-23 0.3127812510915575 1104 24-25 0.20694390016127073 1104 26-27 0.3579933309637511 1104 28-29 0.3232444728138617 1104 30-31 0.30696704655564844 1104 32-33 0.2153059605258676 1104 34-35 0.24449723868229256 1104 36-37 0.25519009841087126 1104 38-39 0.2795256160819406 1104 40-41 0.3132456154666343 1104 42-43 0.41046178526489285 1104 44-45 0.520892730841787 1104 46-47 0.33062970859892715 1104 48-49 0.379890980633661 1104 50-51 0.2648387495499094 1104 52-53 0.217941417006152 1104 54-55 0.09163550017441935 1104 56-57 0.8284309896142013 1104 58-59 -6.179140029423177 1104 60-61 -8.33548311231025 1104 62-63 -2.5094473521074363 1104 64-65 -0.33515197908851135 1104 66-67 -1.733040463440652 1104 68-69 -4.02768045422852 1104 70-71 -4.755777580297375 1104 72-73 -7.822927715383624 1104 74-75 -8.548224597810352 1104 76-77 -9.269838785362996 1104 78-79 -12.260761798558327 1104 80-81 -8.94305599917023 1104 82-83 -5.390098856954754 1104 84-85 -4.4018851577437275 1104 86-87 -4.095481643471107 1104 88-89 -4.413712104864594 1104 90-91 -9.773139422654591 1104 92-93 -14.435269929655849 1104 94-95 -12.164290047331182 1104 96-97 -11.794547045428327 1104 98-99 -11.180881725434007 1104 100-101 -10.805817990061279 1105 1 0.09198419272859581 1105 2 0.15805849892092283 1105 3 0.07441245926190376 1105 4 0.042919794511512066 1105 5 0.012748119630842325 1105 6 0.10419847323053233 1105 7 0.11817083768452363 1105 8 0.2712397411177463 1105 9 0.04714681778953178 1105 10-11 0.18619533731162363 1105 12-13 0.19364657407293606 1105 14-15 0.31892658600705914 1105 16-17 0.2662924961983393 1105 18-19 0.2131479662105491 1105 20-21 0.2949898857473343 1105 22-23 0.3797508115308048 1105 24-25 0.26071332792656676 1105 26-27 0.31095529112170794 1105 28-29 0.14317819989842206 1105 30-31 0.381340973562196 1105 32-33 0.36689840380491034 1105 34-35 0.29200077617976916 1105 36-37 0.23336420165170324 1105 38-39 0.20342208769513093 1105 40-41 0.2521529629271768 1105 42-43 0.20402923402456707 1105 44-45 0.26840740754156656 1105 46-47 0.20301875787335177 1105 48-49 0.30947396164079066 1105 50-51 0.3487788669065637 1105 52-53 0.27226780488670244 1105 54-55 -0.042900439945789515 1105 56-57 0.6598599820039546 1105 58-59 -6.515298022459216 1105 60-61 -8.500572496401336 1105 62-63 -2.260932994280669 1105 64-65 -0.23657662256286116 1105 66-67 -1.5460632585903653 1105 68-69 -3.922430829331372 1105 70-71 -4.577499451854187 1105 72-73 -5.941167856655758 1105 74-75 -6.552771029422054 1105 76-77 -8.474138517452726 1105 78-79 -11.77946782895945 1105 80-81 -7.924839132780871 1105 82-83 -4.182828374346148 1105 84-85 -3.2940676442129764 1105 86-87 -3.08885862254791 1105 88-89 -3.1599945295522005 1105 90-91 -8.584001461676676 1105 92-93 -11.702207211320367 1105 94-95 -11.165162855435112 1105 96-97 -11.514965701492468 1105 98-99 -11.12678274810531 1105 100-101 -10.721979833773645 1106 1 0.10347902463033165 1106 2 0.10470858911605063 1106 3 0.10784522322445866 1106 4 0.055303310894878166 1106 5 0.09336576111375194 1106 6 0.003594083183031671 1106 7 0.1731327619190708 1106 8 0.18362491104645073 1106 9 0.20894776707837082 1106 10-11 0.1527302715332084 1106 12-13 0.1914058278702342 1106 14-15 0.2403920271018194 1106 16-17 0.2784122144188288 1106 18-19 0.22874724372660893 1106 20-21 0.20866405194709614 1106 22-23 0.18180720665584005 1106 24-25 0.14017218021997024 1106 26-27 0.2592172034738027 1106 28-29 0.19731469737407537 1106 30-31 0.2013477569435267 1106 32-33 0.36544655254554215 1106 34-35 0.460624907251308 1106 36-37 0.46654831854120715 1106 38-39 0.32885599914123986 1106 40-41 0.36466999348277085 1106 42-43 0.3623010176577779 1106 44-45 0.3764731325077548 1106 46-47 0.23918256345795186 1106 48-49 0.2541811360663644 1106 50-51 0.5126048324136079 1106 52-53 0.41962119021314237 1106 54-55 0.20163814976461936 1106 56-57 0.9362116303387076 1106 58-59 -6.450653436647329 1106 60-61 -8.78915249703968 1106 62-63 -2.5859894359357085 1106 64-65 -0.21920170653796944 1106 66-67 -1.6029565622316682 1106 68-69 -3.953515558917104 1106 70-71 -4.536845886137488 1106 72-73 -5.885615270645443 1106 74-75 -6.3960037797717035 1106 76-77 -8.446406062626238 1106 78-79 -11.748817058888006 1106 80-81 -7.717656439478535 1106 82-83 -3.8422899415302716 1106 84-85 -2.8070968123681546 1106 86-87 -2.71761212239198 1106 88-89 -2.8048541377850107 1106 90-91 -7.930707426322337 1106 92-93 -11.357905616956572 1106 94-95 -11.630598758826533 1106 96-97 -11.729120587930435 1106 98-99 -11.027632516589735 1106 100-101 -10.636328877197666 1107 1 0.036441859086327355 1107 2 0.08993449771569217 1107 3 0.12479405130739707 1107 4 -0.059812564722790285 1107 5 -0.004784137387900955 1107 6 -0.13940332850941672 1107 7 0.07216124925471235 1107 8 0.07745963424908808 1107 9 -0.006020148277194437 1107 10-11 0.05004325733938941 1107 12-13 0.0754819536244895 1107 14-15 0.04874759435593745 1107 16-17 0.18076362602354124 1107 18-19 0.1446198702804864 1107 20-21 0.1375474106051655 1107 22-23 0.13481486788666075 1107 24-25 0.08045904764997402 1107 26-27 0.33517465791918966 1107 28-29 0.39268485725889235 1107 30-31 0.35506032319661784 1107 32-33 0.2869841428272224 1107 34-35 0.15456013176406458 1107 36-37 0.17383546115336657 1107 38-39 0.276485045615118 1107 40-41 0.13653820116952886 1107 42-43 0.10554549507090627 1107 44-45 0.30335464066551054 1107 46-47 0.28016576139368254 1107 48-49 0.309245883636585 1107 50-51 0.23375317952599772 1107 52-53 0.19191054624700854 1107 54-55 0.13813208977491342 1107 56-57 0.8877564391668678 1107 58-59 -6.377499051480047 1107 60-61 -8.872767194159838 1107 62-63 -2.56742082185675 1107 64-65 0.014782691830081518 1107 66-67 -1.5078821234636877 1107 68-69 -3.9165889049327447 1107 70-71 -4.6277525116464755 1107 72-73 -6.098856717066679 1107 74-75 -6.751136724659496 1107 76-77 -9.140387549646812 1107 78-79 -12.22813711966737 1107 80-81 -8.344633862577087 1107 82-83 -4.618138320149011 1107 84-85 -3.6432838644493692 1107 86-87 -3.2806441910838267 1107 88-89 -3.332947643884882 1107 90-91 -8.562478602387074 1107 92-93 -11.414594936299485 1107 94-95 -11.380860381284588 1107 96-97 -11.894399778177021 1107 98-99 -11.206755363488751 1107 100-101 -10.784504100434209 1108 1 -0.1054433295356354 1108 2 -0.053591180762040835 1108 3 0.016322387561992002 1108 4 -0.08646479691167741 1108 5 -0.20017885161960436 1108 6 -0.11039461054307509 1108 7 -0.06432132054057149 1108 8 -0.014973720850520067 1108 9 -0.09047444482551725 1108 10-11 -0.09046550072272197 1108 12-13 -0.2057417693251793 1108 14-15 -0.09793992211196922 1108 16-17 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1.078125150719849 2312 88-89 1.26981275171498 2312 90-91 2.5752996776893795 2312 92-93 4.538630217760808 2312 94-95 8.516154378365492 2312 96-97 9.165988287206403 2312 98-99 9.46495900833388 2312 100-101 8.652429128209507 2313 1 -0.34991349280784334 2313 2 -0.2675171559695535 2313 3 -0.2047736734147314 2313 4 -0.3904798216352461 2313 5 -0.3968054500466778 2313 6 -0.319170660000232 2313 7 -0.3614265278716715 2313 8 -0.36678726396940675 2313 9 -0.3256115227123715 2313 10-11 -0.4198759427978018 2313 12-13 -0.4547577739052926 2313 14-15 -0.4897918663667937 2313 16-17 -0.5189879124042491 2313 18-19 -0.3971246649607778 2313 20-21 -0.49488699434332517 2313 22-23 -0.4415282403955203 2313 24-25 -0.3848753784357015 2313 26-27 -0.506477152899059 2313 28-29 -0.2852471288783036 2313 30-31 -0.5947841390615523 2313 32-33 -0.4604887676827403 2313 34-35 -0.7204150420194608 2313 36-37 -0.5781568177936052 2313 38-39 -0.7481890230528023 2313 40-41 -0.9536559418374537 2313 42-43 -0.7810090647772299 2313 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2314 68-69 0.2969174157592036 2314 70-71 2.5465040067632447 2314 72-73 8.39638424754498 2314 74-75 9.98523079187975 2314 76-77 8.755644286052856 2314 78-79 6.381655245288254 2314 80-81 3.5036017416860865 2314 82-83 2.0656030022376584 2314 84-85 1.7366499785564393 2314 86-87 1.6390516373850197 2314 88-89 1.7273625533307495 2314 90-91 3.010113320685022 2314 92-93 5.214133142134841 2314 94-95 8.916859273744976 2314 96-97 9.504629835551498 2314 98-99 9.648532802318 2314 100-101 8.888636410995764 2315 1 -0.8288733680033964 2315 2 -0.5747278600131907 2315 3 -0.5233615927482269 2315 4 -0.4891459851053668 2315 5 -0.8270121628933254 2315 6 -0.7112690115195832 2315 7 -0.5493638535722098 2315 8 -0.7482373037936085 2315 9 -1.0438887474371654 2315 10-11 -0.7777221884615741 2315 12-13 -0.5325439870514472 2315 14-15 -0.6569019018465383 2315 16-17 -0.8732787234356678 2315 18-19 -0.8288703693225656 2315 20-21 -0.8621392121016314 2315 22-23 -0.9661165590681406 2315 24-25 -1.0473129268445973 2315 26-27 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4.816752586061291 2315 94-95 8.730296067976582 2315 96-97 9.509223646775645 2315 98-99 9.482977177696425 2315 100-101 8.352093579703327 2316 1 -0.511876908100362 2316 2 -0.5830623167905564 2316 3 -0.7081826156315358 2316 4 -0.4867912464184485 2316 5 -0.6794090931449333 2316 6 -0.6524230132272208 2316 7 -0.5872713516873205 2316 8 -0.7693480653887974 2316 9 -0.9904302874043154 2316 10-11 -0.7729263307245375 2316 12-13 -0.8303268078446493 2316 14-15 -0.9466987831659068 2316 16-17 -1.0224313969828032 2316 18-19 -1.2267338284317049 2316 20-21 -0.970439466249637 2316 22-23 -1.1766896653159833 2316 24-25 -2.483299834976833 2316 26-27 -1.7354003645426204 2316 28-29 -1.411518905435436 2316 30-31 -0.9258566633807419 2316 32-33 -0.6874311693239363 2316 34-35 -0.9120684178833542 2316 36-37 -0.8954803383511134 2316 38-39 -1.1710033150807888 2316 40-41 -0.9764420052068985 2316 42-43 -1.2121476084712626 2316 44-45 -1.1504802037353699 2316 46-47 -1.1516592270350756 2316 48-49 -0.9891096392472392 2316 50-51 -1.0358212711805308 2316 52-53 -0.8998956559365539 2316 54-55 -1.0569834864727383 2316 56-57 0.3571716151858162 2316 58-59 3.6035282021887163 2316 60-61 3.5209788920390785 2316 62-63 1.0804772967960865 2316 64-65 0.8411977861697046 2316 66-67 5.8963965119470245 2316 68-69 8.313786275442435 2316 70-71 4.9906784284825765 2316 72-73 8.975012704915951 2316 74-75 9.943064043126178 2316 76-77 8.35197543328221 2316 78-79 6.153902957655728 2316 80-81 3.217839931972179 2316 82-83 1.7832175566785615 2316 84-85 1.5003260895379427 2316 86-87 1.1449813364284935 2316 88-89 1.2661702193839233 2316 90-91 2.5185704324355456 2316 92-93 4.314531911695287 2316 94-95 8.19412940879954 2316 96-97 8.985144995707373 2316 98-99 9.158893034235119 2316 100-101 8.044703065565413 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 12.0 4 41.0 5 93.0 6 223.0 7 502.0 8 1057.0 9 1884.0 10 3064.0 11 4505.0 12 6390.0 13 8655.0 14 11321.0 15 15886.0 16 21153.0 17 27428.0 18 29024.0 19 29303.0 20 30453.0 21 30716.0 22 29337.0 23 28312.0 24 28681.0 25 30099.0 26 38785.0 27 55897.0 28 78147.0 29 102615.0 30 118551.0 31 123457.0 32 124808.0 33 126127.0 34 125656.0 35 116701.0 36 93118.0 37 52615.0 38 14018.0 39 438.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.83296824202737 20.393436972868358 15.155011913796473 22.618582871307797 2 15.693948715773736 21.880090284837266 38.70346096294107 23.72250003644793 3 19.110803922530163 25.714732549339775 30.78667961983425 24.387783908295816 4 13.125996472008664 17.86691706304661 37.07869859264688 31.928387872297847 5 13.07660632857087 38.481333958397016 34.0705238931035 14.371535819928614 6 30.796675042675233 37.16131503334499 17.44582100787769 14.596188916102083 7 27.308799966071913 33.78263756449319 20.96186137989257 17.946701089542326 8 26.272688657708827 35.10316625681132 19.79803441724666 18.826110668233195 9 26.40314524006112 16.616613896999084 20.389457375847705 36.59078348709208 10-11 24.821115465510637 27.189521521144755 27.156918746198 20.832444267146606 12-13 25.77368637985944 24.66502636716291 27.800856684297788 21.760430568679865 14-15 22.425875778583055 25.792780527629077 26.638631767944727 25.14271192584314 16-17 21.54622636133152 29.217387616511846 27.091746329205858 22.144639692950776 18-19 21.44332520242774 27.50041913592396 29.89678322290013 21.15947243874817 20-21 22.48080611023714 26.39930182350236 29.867289476857994 21.252602589402507 22-23 21.95740566731651 26.533026213426247 29.84098195317128 21.66858616608596 24-25 21.92089321000826 26.662377060369476 29.9240461369025 21.492683592719775 26-27 21.599101964651123 26.966672233001475 29.914477241642544 21.51974856070486 28-29 21.35227909041811 27.354286982826885 29.90709355917153 21.386340367583472 30-31 21.562977562118697 26.834567511792844 29.90272809327712 21.699726832811344 32-33 21.50449814057418 26.932782353081503 29.689318175191577 21.87340133115274 34-35 21.95707924138205 26.95356712125429 29.55061428969027 21.538739347673385 36-37 21.949337663073685 26.5771619453473 29.73985432531582 21.73364606626319 38-39 21.50414758639935 27.05249102093695 29.763990750661932 21.679370642001768 40-41 21.68170087076528 26.83848203866393 29.737332010707828 21.74248507986296 42-43 21.581808666728314 26.898529340117065 29.35397089437054 22.16569109878408 44-45 21.618769498669792 26.91084063896476 29.346448835059608 22.123941027305843 46-47 21.80669354194542 27.286274086081985 28.91542904604913 21.991603325923464 48-49 21.709302163714742 27.187595082207373 28.8929597239227 22.210143030155187 50-51 21.487212170684685 27.431898167987555 28.94494815368661 22.135941507641146 52-53 21.907748719174094 27.50861754980879 28.411550516025606 22.172083214991503 54-55 21.3240437978797 27.281084335872112 28.76634293139324 22.62852893485495 56-57 21.72812462049849 27.32288104606045 28.688256970716917 22.260737362724143 58-59 20.751463769045024 26.72814229544629 30.15764899459367 22.362744940915018 60-61 21.881955525185877 26.91235086905325 29.15503569637426 22.05065790938661 62-63 21.261221322550526 27.224401269922048 28.959046935560934 22.55533047196649 64-65 20.564805010401283 27.18051109464486 29.372263006247408 22.882420888706456 66-67 21.546690743197047 26.592913244489502 29.460516753513833 22.399879258799622 68-69 22.693554656427644 25.522945987258456 29.80789572387348 21.975603632440414 70-71 21.710343986420785 26.21008480232554 29.877522809894398 22.202048401359274 72-73 21.517033562537005 26.134453469313172 29.869114447937083 22.479398520212737 74-75 21.778835633271278 26.58369159397651 29.045977437154292 22.591495335597923 76-77 20.013116257155044 27.08011137759908 29.654771666873874 23.252000698371997 78-79 20.740177704681695 27.449113489987763 28.529392090780544 23.281316714549998 80-81 21.104777568745845 27.73957919862061 27.862469211231 23.293174021402542 82-83 21.3271467497906 27.63675291834982 27.635295068757486 23.400805263102093 84-85 21.30482004195951 27.46187397039509 27.556832865717652 23.676473121927753 86-87 21.306263098166877 27.47569228730234 27.59258799068943 23.625456623841355 88-89 21.27311890375121 27.43895509396991 27.418710884526163 23.869215117752717 90-91 21.14720670576592 27.341505210651118 27.6685906334478 23.842697450135162 92-93 21.032174117975085 27.21240627084945 27.794182568950465 23.961237042225005 94-95 20.145191720425522 26.84394861578991 28.934088895260974 24.076770768523595 96-97 19.8124403468786 26.830215676143688 29.259077597821047 24.098266379156666 98-99 20.10913034205847 26.90928716084673 28.379982235685514 24.60160026140929 100-101 19.703934924639587 27.187175504046913 28.452171147213072 24.656718424100426 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 640.0 1 473.5 2 338.0 3 401.5 4 456.5 5 500.0 6 629.5 7 971.5 8 1373.0 9 1651.0 10 1837.0 11 1934.0 12 2091.0 13 2322.5 14 2481.5 15 2630.5 16 2845.5 17 3008.5 18 3076.5 19 3279.5 20 3620.5 21 4072.5 22 4713.0 23 5481.0 24 6470.5 25 7784.5 26 9453.0 27 11482.5 28 13692.5 29 15850.0 30 18190.5 31 21095.0 32 24173.5 33 27224.5 34 30329.5 35 33242.0 36 36086.0 37 38832.5 38 41258.0 39 43424.5 40 45873.0 41 48851.0 42 51617.5 43 54084.5 44 56365.0 45 58178.5 46 59802.0 47 61490.5 48 62448.0 49 61532.5 50 59622.5 51 56816.5 52 53762.0 53 51068.5 54 47662.5 55 43332.0 56 38281.0 57 33326.5 58 28694.0 59 24094.0 60 19763.0 61 15792.0 62 12024.5 63 8945.5 64 6745.0 65 5139.5 66 3905.5 67 2876.5 68 2013.0 69 1460.0 70 1137.5 71 932.0 72 754.0 73 558.0 74 393.0 75 259.5 76 168.0 77 101.5 78 50.5 79 23.5 80 11.0 81 7.0 82 5.5 83 3.5 84 1.5 85 1.5 86 1.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021138791073201182 2 0.00477113779708616 3 0.0031807585313907735 4 0.0 5 0.06368143643055278 6 1.3253160547461556E-4 7 0.0 8 3.313290136865389E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 1.6566450684326944E-4 16-17 0.0 18-19 0.0011265186465342321 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.3253160547461556E-4 28-29 0.0019217082793819254 30-31 0.00715670669562924 32-33 3.975948164238467E-4 34-35 9.277212383223089E-4 36-37 0.029057554500309463 38-39 0.02819609906472446 40-41 0.030382870555055615 42-43 0.03084673117421677 44-45 0.03164192080706447 46-47 0.03316603427002254 48-49 0.03386182519876428 50-51 0.028659959683885614 52-53 0.025147872138808303 54-55 0.018521291865077526 56-57 0.14333293132079672 58-59 0.766695337670651 60-61 0.30810284982711256 62-63 3.313290136865389E-4 64-65 0.15164928956432885 66-67 1.4310100101121614 68-69 2.7404554051027317 70-71 2.2059223073222385 72-73 1.8990785077471348 74-75 1.5830237615915455 76-77 1.1286722851231947 78-79 0.43983926566888043 80-81 6.626580273730778E-5 82-83 1.3253160547461556E-4 84-85 0.0 86-87 0.002418701799911734 88-89 3.313290136865389E-5 90-91 0.23219537279152644 92-93 0.181502033697486 94-95 1.3213069736805483 96-97 1.3077224841194004 98-99 1.0357676296854892 100-101 0.4149895896423899 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1509074.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.13468782722369 #Duplication Level Percentage of deduplicated Percentage of total 1 76.78031344433886 41.564782995856696 2 12.971667924012136 14.044343873296155 3 4.361238267040132 7.082828163790787 4 1.9539720733643775 4.231106728587744 5 1.0125235840427615 2.740632406992829 6 0.6416828467849173 2.084238035687142 7 0.4145631639880602 1.5709573227011844 8 0.28087849939180204 1.2164215905563391 9 0.2086788329176793 1.0167087128533145 >10 1.204860539857932 12.167427531767999 >50 0.1125373789278718 4.219079610702707 >100 0.05304113218933626 5.159464151827642 >500 0.002939864104878773 1.019413258716448 >1k 8.574603639229755E-4 0.9418946156773842 >5k 2.449886754065644E-4 0.9407010009855936 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7656 0.5073309857568283 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6530 0.4327156918746198 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4772 0.3162204106624327 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2605 0.17262241613068677 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1566 0.103772247086624 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.3253160547461556E-4 0.0 0.0 0.0 0.0 7 1.3253160547461556E-4 0.0 0.0 0.0 0.0 8 1.3253160547461556E-4 0.0 0.0 0.0 0.0 9 1.3253160547461556E-4 0.0 0.0 0.0 0.0 10-11 1.3253160547461556E-4 0.0 0.0 1.3253160547461556E-4 0.0 12-13 1.9879740821192334E-4 0.0 0.0 1.9879740821192334E-4 0.0 14-15 2.98196112317885E-4 0.0 0.0 1.9879740821192334E-4 0.0 16-17 4.6386061916115444E-4 0.0 0.0 1.9879740821192334E-4 0.0 18-19 5.301264218984623E-4 0.0 0.0 1.9879740821192334E-4 0.0 20-21 5.9639222463577E-4 0.0 0.0 2.3193030958057725E-4 0.0 22-23 6.626580273730778E-4 0.0 0.0 4.3072771779250057E-4 0.0 24-25 7.951896328476934E-4 0.0 0.0 0.001225917350640194 0.0 26-27 8.614554355850011E-4 0.0 0.0 0.002716897912229619 0.0 28-29 8.945883369536551E-4 0.0 0.0 0.00937661108732905 0.0 30-31 0.0013253160547461555 0.0 0.0 0.031244325990640615 0.0 32-33 0.001524113462958079 0.0 0.0 0.0796846277916126 0.0 34-35 0.0016566450684326944 0.0 0.0 0.15052277091779462 0.0 36-37 0.0017229108711700023 0.0 0.0 0.2698343487463173 0.0 38-39 0.0017229108711700023 0.0 0.0 0.4480893581096752 0.0 40-41 0.0017229108711700023 0.0 0.0 0.6378083513465873 0.0 42-43 0.0017560437725386562 0.0 0.0 0.8300123121861486 0.0 44-45 0.0019548411807505796 0.0 0.0 1.0665480950569686 0.0 46-47 0.002153638588962503 0.0 0.0 1.3319095021185179 0.0 48-49 0.002186771490331157 0.0 0.0 1.5938250874377267 0.0 50-51 0.002186771490331157 0.0 0.0 1.845966466853183 0.0 52-53 0.002186771490331157 0.0 0.0 2.0860806030718173 0.0 54-55 0.002186771490331157 0.0 0.0 2.345279290478797 0.0 56-57 0.002186771490331157 3.313290136865389E-5 0.0 2.5737637783170344 0.0 58-59 0.002186771490331157 6.626580273730778E-5 0.0 2.722928100278714 0.0 60-61 0.002186771490331157 6.626580273730778E-5 0.0 2.82935097947483 0.0 62-63 0.002186771490331157 6.626580273730778E-5 0.0 2.9201019963235733 0.0 64-65 0.002186771490331157 1.3253160547461556E-4 0.0 3.014133170407813 0.0 66-67 0.002186771490331157 1.3253160547461556E-4 0.0 3.0924262163419423 0.0 68-69 0.002186771490331157 1.3253160547461556E-4 0.0 3.1857947323988087 0.0 70-71 0.002186771490331157 1.3253160547461556E-4 0.0 3.3594111355705554 0.0 72-73 0.002186771490331157 1.3253160547461556E-4 0.0 3.5771605633653483 0.0 74-75 0.002186771490331157 1.3253160547461556E-4 0.0 3.82916941117533 0.0 76-77 0.002186771490331157 1.3253160547461556E-4 0.0 4.196845217663283 0.0 78-79 0.0022530372930684643 1.3253160547461556E-4 0.0 4.734360276566955 0.0 80-81 0.00238556889854308 1.3253160547461556E-4 0.0 5.328499463909656 0.0 82-83 0.00238556889854308 1.3253160547461556E-4 0.0 5.8777767027992 0.0 84-85 0.0024187017999117337 1.3253160547461556E-4 0.0 6.314368944133952 0.0 86-87 0.002451834701280388 1.3253160547461556E-4 0.0 6.696291898210426 0.0 88-89 0.002451834701280388 1.3253160547461556E-4 0.0 7.0821245346484005 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6670 0.0 32.46911 1 GTATCAA 9745 0.0 27.646347 1 TACATGG 7390 0.0 26.108143 2 GTACATG 7730 0.0 25.208912 1 ACATGGG 7625 0.0 24.003502 3 TCAACGC 11785 0.0 22.77461 4 TATCAAC 11830 0.0 22.689486 2 CAACGCA 11855 0.0 22.640135 5 ATCAACG 11875 0.0 22.602755 3 AACGCAG 11985 0.0 22.417566 6 GAGTACT 6460 0.0 21.92621 12-13 CATGGGG 3110 0.0 21.234201 4 GGACCGA 270 1.1023076E-9 20.949282 6 GTACTTT 6815 0.0 20.541975 14-15 CTCGCGA 60 0.004135888 20.282059 64-65 CGCAGAG 13495 0.0 19.804403 8 AGTACTT 6610 0.0 19.788511 12-13 ACGCAGA 13550 0.0 19.724018 7 CATGGGA 4665 0.0 19.717474 4 GTAGGAC 410 0.0 19.55905 3 >>END_MODULE