FastQCFastQC Report
Fri 27 May 2016
SRR939754_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939754_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1579895
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76070.4814876938024362No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56540.3578718838910181No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38980.2467252570582222No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24850.1572889337582561No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA108850.029.7222351
GTACATG79400.026.4733241
GGTATCA74450.026.149121
TACATGG78950.026.148552
TATCAAC127100.025.0483042
ATCAACG129100.024.8423883
TCAACGC130000.024.5988354
ACATGGG80500.024.5935613
CAACGCA131350.024.311755
ATGGGCG1951.7456841E-824.1180345
AACGCAG133800.023.9376376
GAGTACT64700.022.20596712-13
AGTACTT66000.021.48355512-13
ACGCAGA147750.021.4547147
CGCAGAG149250.021.1753928
GTACTTT67700.021.16053414-15
CATGGGA50100.021.1199934
GCAGAGT148850.020.0000659
AGAGTAC109900.019.72753710-11
CATGGGG34650.019.5437644
Produced by FastQC (version 0.11.5)