FastQCFastQC Report
Fri 27 May 2016
SRR939754_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939754_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1579895
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80320.5083882156725605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68910.43616822636947394No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48500.3069824260473006No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26820.16975811683687841No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15940.10089278084936024No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71650.029.736581
GTATCAA100600.028.0659581
GTACATG84500.023.9228951
TACATGG83850.023.536462
TATCAAC124550.022.3967232
ACATGGG85150.022.3364623
ATCAACG125600.022.091283
TCAACGC126250.021.9399744
CAACGCA126650.021.8332335
AGTACTT67150.021.75501412-13
GAGTACT66550.021.6660712-13
AACGCAG128900.021.5625136
GCGGTAT1805.215583E-621.0900881
AGAGTAC112950.020.53417610-11
GTGTAGC6500.020.4411621
GTACTTT69850.020.4387714-15
GAGTACA67900.019.7777791
GTAAGGT4900.019.3592514
ACGCAGA143650.019.2494167
CGCAGAG144400.019.1165928
Produced by FastQC (version 0.11.5)