FastQCFastQC Report
Fri 27 May 2016
SRR939753_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939753_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2887553
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183390.6351052257742109No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131220.45443321732969055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92640.32082528009009703No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37560.130075534544301No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29570.10240504676450961No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108950.041.36591
GTATCAA186350.038.5936551
TATCAAC231550.031.6131112
ATCAACG236450.030.8603443
TCAACGC237450.030.7503624
CAACGCA241050.030.251755
AACGCAG247800.029.6574576
TACATGG185650.028.5944162
GTACATG183850.028.3066921
ACATGGG191200.026.9727613
ACGCAGA278050.026.1749737
CGCAGAG280000.025.9244568
CATGGGA130100.024.5791234
GCAGAGT283850.024.1851259
GAGTACT156500.023.72028412-13
ATGGGAT52350.022.294735
CATGGGG58550.022.040724
GTACTTT168350.021.89754514-15
AGTACTT163350.021.36059812-13
ATGGGAG48500.020.9341745
Produced by FastQC (version 0.11.5)