##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939753_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2887553 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.845529415390818 31.0 30.0 33.0 16.0 34.0 2 29.67325621382534 31.0 30.0 33.0 25.0 34.0 3 29.906720673178985 31.0 30.0 33.0 25.0 34.0 4 33.70792917047756 35.0 33.0 37.0 28.0 37.0 5 33.688959821689856 35.0 33.0 37.0 28.0 37.0 6 33.73848202959392 35.0 35.0 37.0 28.0 37.0 7 33.661389072339105 35.0 33.0 37.0 28.0 37.0 8 33.52996533743277 35.0 33.0 37.0 28.0 37.0 9 34.767921489233274 37.0 34.0 39.0 27.0 39.0 10-11 34.61724304281168 37.0 34.0 39.0 27.0 39.0 12-13 34.49688057673747 37.0 33.5 39.0 27.0 39.0 14-15 35.09970241238862 38.0 33.0 40.0 26.5 41.0 16-17 34.96522245652287 38.0 33.0 40.0 26.0 41.0 18-19 34.89706699755814 38.0 33.0 40.0 25.5 41.0 20-21 35.08538163628512 38.0 34.0 40.0 25.5 41.0 22-23 34.98028728823333 38.0 34.0 40.0 25.0 41.0 24-25 34.7502598220708 38.0 33.0 40.0 24.5 41.0 26-27 34.601644541243054 38.0 33.0 40.0 24.5 41.0 28-29 34.62057995125977 38.0 33.0 40.0 24.0 41.0 30-31 34.33385326606992 38.0 33.0 40.0 24.0 41.0 32-33 33.90789485076118 38.0 33.0 40.0 22.0 41.0 34-35 33.68703708641884 37.5 32.5 40.0 21.0 41.0 36-37 33.435196860455896 37.0 32.0 40.0 19.0 41.0 38-39 33.166920745697134 37.0 31.5 40.0 17.5 41.0 40-41 32.88523258274394 37.0 31.0 40.0 16.0 41.0 42-43 32.4205418913523 37.0 31.0 40.0 15.0 41.0 44-45 31.976552118697043 36.5 30.0 40.0 9.0 41.0 46-47 31.473958746384916 36.0 30.0 39.0 8.0 41.0 48-49 31.030235289187765 36.0 29.0 39.0 4.0 40.0 50-51 30.372295677343413 35.5 28.0 38.5 2.0 40.0 52-53 30.49960918466258 35.5 28.0 39.0 2.0 40.0 54-55 30.59278998515352 36.0 28.0 39.0 2.0 40.0 56-57 30.285290347917424 35.0 28.0 39.0 2.0 40.0 58-59 29.885420458083367 35.0 27.5 39.0 2.0 40.0 60-61 29.47630588945034 35.0 26.5 38.0 2.0 40.0 62-63 29.026304971718268 34.0 26.0 38.0 2.0 40.0 64-65 28.478826189510634 34.0 25.5 38.0 2.0 40.0 66-67 27.88160546317245 34.0 24.5 37.0 2.0 39.5 68-69 27.35167544976664 33.0 22.5 36.5 2.0 39.0 70-71 26.768572213219983 32.5 22.0 36.0 2.0 39.0 72-73 26.100095132452978 32.0 20.0 35.5 2.0 38.5 74-75 25.748004625369646 32.0 19.5 35.0 2.0 38.0 76-77 25.17381654986073 31.0 17.5 35.0 2.0 37.0 78-79 24.49925473229409 31.0 10.5 35.0 2.0 36.5 80-81 23.760980491094017 30.5 6.5 34.0 2.0 36.0 82-83 23.01868935392701 30.0 2.0 34.0 2.0 35.5 84-85 22.271305669540958 29.0 2.0 34.0 2.0 35.0 86-87 21.441029653828 29.0 2.0 34.0 2.0 35.0 88-89 20.497027413869112 27.5 2.0 33.5 2.0 35.0 90-91 19.12662243775266 25.5 2.0 32.5 2.0 34.5 92-93 17.62024662404465 21.5 2.0 32.0 2.0 34.0 94-95 17.07464278577744 20.0 2.0 32.0 2.0 34.0 96-97 16.682760801273606 18.5 2.0 32.0 2.0 34.0 98-99 15.780924713762829 4.0 2.0 31.5 2.0 34.0 100-101 14.169241049428358 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.542338613132575 1101 2 1.0698996229353313 1101 3 1.0689371091255175 1101 4 0.704887586890365 1101 5 0.9383133577998208 1101 6 0.9898507281228603 1101 7 0.9507873750310978 1101 8 1.1102676016795954 1101 9 1.0738924323352919 1101 10-11 1.1954082384315612 1101 12-13 1.186992843912293 1101 14-15 1.3609956489382355 1101 16-17 1.2507993457628004 1101 18-19 1.2874927919935928 1101 20-21 1.062480571342249 1101 22-23 1.240002049012375 1101 24-25 1.4939201540388396 1101 26-27 1.313352519302292 1101 28-29 1.1974162293976605 1101 30-31 1.4398655208790387 1101 32-33 1.5346777218568377 1101 34-35 1.4588515035331397 1101 36-37 1.4511313901695289 1101 38-39 1.53609784139163 1101 40-41 1.3498943811964637 1101 42-43 1.261781185109193 1101 44-45 1.030275168877104 1101 46-47 1.1416506811329654 1101 48-49 0.9616069290519711 1101 50-51 1.0013603812203549 1101 52-53 0.924840175164718 1101 54-55 1.0535122719984429 1101 56-57 1.15319063266028 1101 58-59 0.7731577960595573 1101 60-61 0.8033513483273822 1101 62-63 0.4776372581615327 1101 64-65 0.6425957785470153 1101 66-67 0.6456518573076586 1101 68-69 0.6575629611398632 1101 70-71 0.6842111098961041 1101 72-73 0.5732834861160434 1101 74-75 0.5669812499611488 1101 76-77 0.18949689880794196 1101 78-79 0.051179226141293555 1101 80-81 -0.12360769319850107 1101 82-83 -0.3601661067673554 1101 84-85 -0.3630481617170922 1101 86-87 -0.6303695776055882 1101 88-89 -4.6015375976278285 1101 90-91 -7.733792503559069 1101 92-93 -10.118341974033486 1101 94-95 -8.207622766572934 1101 96-97 -4.91952564598834 1101 98-99 -3.481956242711087 1101 100-101 -2.759423519828294 1102 1 1.942556096778823 1102 2 1.2294182804535616 1102 3 1.0641172877299638 1102 4 1.1530624133582066 1102 5 1.0942895105580916 1102 6 1.0664887267935725 1102 7 1.101518333968066 1102 8 1.1875016814469177 1102 9 1.4921162825754095 1102 10-11 1.4357262682855918 1102 12-13 1.2468377268998907 1102 14-15 1.479244185970927 1102 16-17 1.5133578769486178 1102 18-19 1.7117636071075069 1102 20-21 1.4875814423762534 1102 22-23 1.395256487887579 1102 24-25 1.4781641834830808 1102 26-27 1.5267092734388328 1102 28-29 1.4626645215613578 1102 30-31 1.6218663504352264 1102 32-33 1.777598759101906 1102 34-35 1.8020820751773101 1102 36-37 1.6893452457567903 1102 38-39 1.6590735493878555 1102 40-41 1.4438633078207204 1102 42-43 1.5778072111852381 1102 44-45 1.5338454587720136 1102 46-47 1.6170468441857366 1102 48-49 1.4110207926349538 1102 50-51 1.4894169709448484 1102 52-53 1.5787108543513497 1102 54-55 1.6871592708540533 1102 56-57 1.5737073562131805 1102 58-59 1.386435692385021 1102 60-61 1.340402426000825 1102 62-63 1.3378406493472461 1102 64-65 1.5052658500587555 1102 66-67 1.6541313207946935 1102 68-69 1.460329880837822 1102 70-71 1.5990739582722249 1102 72-73 1.515268624067243 1102 74-75 1.3953012987350562 1102 76-77 1.4149083037063193 1102 78-79 1.3922316630875287 1102 80-81 1.4349789629404661 1102 82-83 1.1087132427806345 1102 84-85 1.1217132909057526 1102 86-87 1.1090207485699075 1102 88-89 0.5617762294746704 1102 90-91 -5.1292770193431245 1102 92-93 -9.649598651710928 1102 94-95 -7.293048363878377 1102 96-97 -3.8193692296710715 1102 98-99 -2.4076551937722694 1102 100-101 -1.7565650491101295 1103 1 1.9683950229533203 1103 2 1.2608434679782334 1103 3 0.953497469332806 1103 4 0.8115701117395702 1103 5 0.8953357008462888 1103 6 0.8501642104098366 1103 7 0.7175072798820068 1103 8 0.907616170195439 1103 9 1.074390265994282 1103 10-11 0.8492872631533359 1103 12-13 0.6806119077131569 1103 14-15 1.0381071942614923 1103 16-17 0.8538237277184564 1103 18-19 0.7316313528635234 1103 20-21 0.656018078775773 1103 22-23 0.8356927294983123 1103 24-25 0.6414488261992304 1103 26-27 0.791119064200771 1103 28-29 0.7145298985846509 1103 30-31 0.8992844317617141 1103 32-33 0.8034080761173072 1103 34-35 0.8161168165906503 1103 36-37 0.8432437850382755 1103 38-39 0.9623700357676412 1103 40-41 0.5630895217962362 1103 42-43 0.6579888140277106 1103 44-45 0.6849706068178065 1103 46-47 0.7857697024565553 1103 48-49 0.7022071329428883 1103 50-51 0.6126206819594593 1103 52-53 0.5786121571543532 1103 54-55 0.400424174130805 1103 56-57 0.5809023818744521 1103 58-59 0.5393373899768079 1103 60-61 0.6571118614528579 1103 62-63 0.4932591254625258 1103 64-65 0.3583748117642429 1103 66-67 0.4288648383627951 1103 68-69 0.14615696334868034 1103 70-71 0.21492472811035768 1103 72-73 0.2026255131315935 1103 74-75 0.1221864151977492 1103 76-77 0.28908911697122264 1103 78-79 0.08645787706305086 1103 80-81 -0.08669494152064772 1103 82-83 -0.5387601089761738 1103 84-85 -0.6803593502312388 1103 86-87 -0.5114786592469116 1103 88-89 -1.194155472065006 1103 90-91 -6.340785112513279 1103 92-93 -9.598068845157435 1103 94-95 -8.365659891710985 1103 96-97 -5.277768361135738 1103 98-99 -3.7628367966147493 1103 100-101 -2.8646779587034974 1104 1 1.9185013641541353 1104 2 1.119106091031771 1104 3 0.9356074376069436 1104 4 0.7921639457149254 1104 5 0.6498126212757143 1104 6 0.7003018803461671 1104 7 0.6673199693436374 1104 8 0.791527099511633 1104 9 0.8621791191419348 1104 10-11 0.8374478692540563 1104 12-13 0.7393323274299703 1104 14-15 0.7476256142342237 1104 16-17 0.777980830295796 1104 18-19 0.7482632711087618 1104 20-21 0.7488329877238726 1104 22-23 0.7886792184295643 1104 24-25 0.7688054310467933 1104 26-27 0.8267257164826987 1104 28-29 0.7224495602343026 1104 30-31 0.9200183184222936 1104 32-33 0.5959373861349988 1104 34-35 0.6081231352877268 1104 36-37 0.706623030412878 1104 38-39 0.6171891454138034 1104 40-41 0.6298241409314755 1104 42-43 0.5729354889898772 1104 44-45 0.5528288685583647 1104 46-47 0.3210812689374549 1104 48-49 0.16849796509587733 1104 50-51 0.22691552066059373 1104 52-53 0.1619984404631971 1104 54-55 0.11680049843175055 1104 56-57 0.22295095023948974 1104 58-59 0.2413574315425393 1104 60-61 0.2011235501599522 1104 62-63 0.15730245177998725 1104 64-65 0.1670620274339072 1104 66-67 0.16752162985902785 1104 68-69 0.02652708367750023 1104 70-71 -0.0433954780415462 1104 72-73 -0.004077843455608132 1104 74-75 -0.26384141001438266 1104 76-77 -0.4369538872338872 1104 78-79 -0.4170960591057522 1104 80-81 -0.25556035592039095 1104 82-83 -0.21715784271722427 1104 84-85 -0.4370632583806824 1104 86-87 -0.3508739925890687 1104 88-89 -0.7174641615972348 1104 90-91 -3.282273183325046 1104 92-93 -7.793754635590258 1104 94-95 -5.4809969624300905 1104 96-97 -3.0925796827230023 1104 98-99 -2.120918617564408 1104 100-101 -1.578646382868099 1105 1 1.8939940308710703 1105 2 1.1806833124139757 1105 3 0.9495120354768751 1105 4 0.8336376399513483 1105 5 0.730228052720399 1105 6 0.6817031082262659 1105 7 0.8459697774625639 1105 8 0.7147693802837125 1105 9 0.7863659230687219 1105 10-11 0.8021277962850135 1105 12-13 0.7692905117760134 1105 14-15 0.8791464949425389 1105 16-17 0.7128573477833626 1105 18-19 0.7666705590176761 1105 20-21 0.7082545400490119 1105 22-23 0.8174541031091067 1105 24-25 0.862088998328197 1105 26-27 0.6224166933762731 1105 28-29 0.6754793762110296 1105 30-31 0.9924301067062373 1105 32-33 1.0605409179107923 1105 34-35 0.8141727134933205 1105 36-37 0.8493330049213341 1105 38-39 0.834927528374287 1105 40-41 0.7439543528406745 1105 42-43 0.7649885304470345 1105 44-45 0.8499283622992451 1105 46-47 0.7402375610623295 1105 48-49 0.6186353442690091 1105 50-51 0.5991439603321531 1105 52-53 0.4599448963186461 1105 54-55 0.479833065960527 1105 56-57 0.6241847832176326 1105 58-59 0.5879359162575355 1105 60-61 0.5261744472585228 1105 62-63 0.35954847228169484 1105 64-65 0.6092513236318702 1105 66-67 0.5261636198661357 1105 68-69 0.5324541649025214 1105 70-71 0.29890917168455644 1105 72-73 0.3938557806426992 1105 74-75 0.2825215805522916 1105 76-77 0.2354162970539555 1105 78-79 0.05869656647018928 1105 80-81 0.027331273937651446 1105 82-83 -0.1683839369552338 1105 84-85 -0.3240753374295977 1105 86-87 -0.3482243008819559 1105 88-89 -0.4141518485341109 1105 90-91 -3.221515805395404 1105 92-93 -7.693347789517105 1105 94-95 -5.462313527170274 1105 96-97 -2.865462872724285 1105 98-99 -1.864584756409311 1105 100-101 -1.374098913965268 1106 1 1.7016243425127655 1106 2 1.094806112654485 1106 3 0.8576262746122971 1106 4 0.832284725581431 1106 5 0.869581927779322 1106 6 0.9078828914330188 1106 7 0.9242063644860963 1106 8 -0.037298172501003535 1106 9 -0.37574810780544965 1106 10-11 -0.6012875203224795 1106 12-13 -0.018287264284914784 1106 14-15 0.7125959955074208 1106 16-17 0.9294620959160085 1106 18-19 1.0133181126757407 1106 20-21 0.8995968622067707 1106 22-23 0.9376436880309953 1106 24-25 0.9827234337666511 1106 26-27 0.8814267358235028 1106 28-29 0.7669122248330922 1106 30-31 0.9523572264642937 1106 32-33 1.039476859660347 1106 34-35 0.9935025041459795 1106 36-37 0.8988983435306181 1106 38-39 0.9019354187885611 1106 40-41 0.7127696374251968 1106 42-43 0.9557513040838543 1106 44-45 0.9016751815564632 1106 46-47 0.8532734279050089 1106 48-49 0.809441505667003 1106 50-51 0.7921175503418851 1106 52-53 0.8197663178920465 1106 54-55 0.9157763830696339 1106 56-57 0.9623751384822192 1106 58-59 0.8837133633552625 1106 60-61 0.6396109370934688 1106 62-63 0.97232798743606 1106 64-65 0.7448032551430295 1106 66-67 0.8631176758660182 1106 68-69 0.7585232113459028 1106 70-71 0.8257968752011458 1106 72-73 0.786720649320543 1106 74-75 0.5096308135798395 1106 76-77 0.6066541862701662 1106 78-79 0.4440098932869603 1106 80-81 0.28841234019047945 1106 82-83 0.20939117224196124 1106 84-85 0.2562155901055654 1106 86-87 0.20677967959522903 1106 88-89 0.0924992993101057 1106 90-91 -3.2847056288100163 1106 92-93 -8.270076721819882 1106 94-95 -5.826814668688275 1106 96-97 -2.8744420678729163 1106 98-99 -1.813285250973804 1106 100-101 -1.1009716696983318 1107 1 1.9239872054896026 1107 2 1.1792448484604812 1107 3 0.9942054494773949 1107 4 0.8331621010785994 1107 5 0.7199959041864048 1107 6 0.7922598079272518 1107 7 0.679363334750029 1107 8 0.7034416823328371 1107 9 0.8101734723223686 1107 10-11 0.686877412727533 1107 12-13 0.7479735356635331 1107 14-15 0.9572923409048002 1107 16-17 0.8251429356975066 1107 18-19 0.9229123934064134 1107 20-21 0.6656227269262445 1107 22-23 0.7252341165229197 1107 24-25 0.8153239461242308 1107 26-27 0.8682492286599484 1107 28-29 0.6561316904892394 1107 30-31 0.801975142705686 1107 32-33 1.139162138199488 1107 34-35 1.1129939365463954 1107 36-37 0.8028725279607798 1107 38-39 0.8151531740376043 1107 40-41 0.9531119809778303 1107 42-43 0.8730906926515374 1107 44-45 0.7517206995744488 1107 46-47 0.867579137508244 1107 48-49 1.0188978943815918 1107 50-51 0.9012297824286186 1107 52-53 0.9567337748301448 1107 54-55 0.9420324974200724 1107 56-57 0.8993919634926115 1107 58-59 0.84534033623504 1107 60-61 0.4497086192515525 1107 62-63 0.7609500120403645 1107 64-65 0.7706678107784981 1107 66-67 0.8301017399178114 1107 68-69 0.8169527823843197 1107 70-71 0.8101985303422268 1107 72-73 0.5999344179885249 1107 74-75 0.5654639189135011 1107 76-77 0.4892803383511328 1107 78-79 0.4901403156515727 1107 80-81 0.3352081393170394 1107 82-83 0.0922595773856365 1107 84-85 0.057667688603604716 1107 86-87 0.049608393682550656 1107 88-89 0.04566330670876084 1107 90-91 -2.7719426714658866 1107 92-93 -3.987483226937176 1107 94-95 -2.355687345480856 1107 96-97 -0.8754765037416625 1107 98-99 -0.3929898944669663 1107 100-101 -0.0290847662724385 1108 1 1.551429486093248 1108 2 0.9450342532236711 1108 3 -0.5900402968319973 1108 4 -0.27585521461192997 1108 5 0.20005212536136696 1108 6 0.3134363649092222 1108 7 0.3844475304272379 1108 8 0.42831143849377895 1108 9 0.5828422167281104 1108 10-11 0.47353315149318576 1108 12-13 0.5363603299524726 1108 14-15 0.3640467388971089 1108 16-17 0.4129041487355991 1108 18-19 0.4251662216380865 1108 20-21 0.27127257499863333 1108 22-23 0.1501425414313431 1108 24-25 0.2828214516562255 1108 26-27 0.31585276769766324 1108 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1.1234025175048892 2311 86-87 1.6628123458275716 2311 88-89 1.879356968596312 2311 90-91 2.6208493612140096 2311 92-93 3.641329992622886 2311 94-95 3.3298671994659657 2311 96-97 2.464531933511502 2311 98-99 2.1586990521330005 2311 100-101 2.1147448191652227 2312 1 1.2276215975532168 2312 2 0.48063325243873933 2312 3 -0.28626037140028515 2312 4 -0.7529929206558847 2312 5 -0.21886957793532957 2312 6 -0.44252664529486196 2312 7 -0.1096924813466984 2312 8 0.08999952719079118 2312 9 0.0029915985962460923 2312 10-11 -0.22374105867164928 2312 12-13 -0.02461948264564029 2312 14-15 0.04708551931312144 2312 16-17 -0.3048131785799413 2312 18-19 -0.5488004924513916 2312 20-21 0.1870662121650355 2312 22-23 0.3336075139588175 2312 24-25 0.4400139801099172 2312 26-27 0.33866661679499543 2312 28-29 0.3932959914473244 2312 30-31 0.2829413028897534 2312 32-33 0.4516963440574173 2312 34-35 0.5382523677006148 2312 36-37 0.3672826499066346 2312 38-39 0.36130665650847504 2312 40-41 0.11205982058302055 2312 42-43 0.2889169567742371 2312 44-45 0.11399725079044742 2312 46-47 0.2604778932799974 2312 48-49 0.32473512403736393 2312 50-51 0.1725246491122725 2312 52-53 0.13520942419100734 2312 54-55 0.18715280571314707 2312 56-57 0.14621278455901532 2312 58-59 0.1308999303097984 2312 60-61 0.08447537461754706 2312 62-63 0.23764638669122817 2312 64-65 0.30497756408742305 2312 66-67 0.4251761848393052 2312 68-69 0.2886618839733579 2312 70-71 0.4675810587763358 2312 72-73 0.12382937219199519 2312 74-75 0.3222424780526758 2312 76-77 0.6086177453269279 2312 78-79 0.577306523487934 2312 80-81 0.8541266818014996 2312 82-83 1.0058142973222246 2312 84-85 1.1827335447093894 2312 86-87 1.482650672516126 2312 88-89 1.7657063471141363 2312 90-91 2.296843146392245 2312 92-93 3.375880883804875 2312 94-95 3.1558309365672805 2312 96-97 2.3753638988023127 2312 98-99 1.945318130463205 2312 100-101 1.8939662075329533 2313 1 -7.15551532805862 2313 2 -2.559626129618689 2313 3 -1.5918401318510398 2313 4 -0.7644751028124048 2313 5 -0.5153384157931029 2313 6 -0.28279870191559553 2313 7 -0.20656251234176892 2313 8 -0.18506622210634305 2313 9 -0.21172648598918187 2313 10-11 -0.12125974285033436 2313 12-13 -0.27319638483834296 2313 14-15 -0.3587544477618181 2313 16-17 -0.5171326380674302 2313 18-19 -0.5893220975408084 2313 20-21 -0.2468657362322091 2313 22-23 -0.937937443293329 2313 24-25 -0.35888976018252094 2313 26-27 -0.4067880939657158 2313 28-29 -0.6312788885764107 2313 30-31 -0.15820146382339573 2313 32-33 -0.3572108493333701 2313 34-35 -0.14110890632343853 2313 36-37 -0.15801696668783194 2313 38-39 -0.1907176548882532 2313 40-41 -0.23374457489791922 2313 42-43 -0.24431956497992502 2313 44-45 -0.32077510757130767 2313 46-47 -1.3450138113623495 2313 48-49 -0.9343363705817076 2313 50-51 -1.03599451180283 2313 52-53 -1.285070633247809 2313 54-55 -0.980127214998241 2313 56-57 -0.7722451243684851 2313 58-59 -0.6413623820202474 2313 60-61 -0.319673960543998 2313 62-63 -0.2199838444467197 2313 64-65 -0.45637571199913296 2313 66-67 -0.42375679336620564 2313 68-69 -0.475488437787714 2313 70-71 -0.343628800305563 2313 72-73 -0.3698700735839182 2313 74-75 -0.22985555825418302 2313 76-77 -0.004461053729393427 2313 78-79 -0.0748293744760673 2313 80-81 0.06719518277513714 2313 82-83 0.4407642616030003 2313 84-85 0.6246339946563886 2313 86-87 1.080817595794688 2313 88-89 1.5622178536012115 2313 90-91 2.0023341848998726 2313 92-93 3.015081435724497 2313 94-95 2.6073526223130052 2313 96-97 1.8588700430849308 2313 98-99 1.482423094571148 2313 100-101 1.241463845455245 2314 1 0.7268772741055791 2314 2 0.170063569862279 2314 3 -1.2005313978136307 2314 4 -0.11285375823698018 2314 5 -0.06781825726805124 2314 6 -3.3419054103551034 2314 7 -2.51117388774151 2314 8 -2.1811644556637013 2314 9 -2.845507445657695 2314 10-11 -2.063971813421418 2314 12-13 -0.9533544948096804 2314 14-15 -1.1314822372902213 2314 16-17 -2.0720788759849498 2314 18-19 -3.1768701026228428 2314 20-21 -2.8684543074132733 2314 22-23 -3.9593040360875165 2314 24-25 -3.0501689873367965 2314 26-27 -1.9412543893449552 2314 28-29 -1.7381122134878026 2314 30-31 -1.6964178404142416 2314 32-33 -1.8854705929212656 2314 34-35 -1.2546794557470236 2314 36-37 -1.765831778962692 2314 38-39 -3.253203668445181 2314 40-41 -2.396061848786765 2314 42-43 -1.4781749686196513 2314 44-45 -2.011359343857375 2314 46-47 -1.3415085380221363 2314 48-49 -2.0028779725798707 2314 50-51 -2.7480387342287855 2314 52-53 -1.8846954356329029 2314 54-55 -2.282695216182127 2314 56-57 -1.3985914323084536 2314 58-59 -0.7071876363028053 2314 60-61 -1.0068678830284234 2314 62-63 -0.7733910322151694 2314 64-65 -1.4538724996289787 2314 66-67 -0.7600977232932244 2314 68-69 -0.7692034716800258 2314 70-71 -0.6385168907139338 2314 72-73 -1.7953347844345124 2314 74-75 -1.771847101419052 2314 76-77 -0.9528986326851197 2314 78-79 -1.8664995903906565 2314 80-81 -0.7916799823551273 2314 82-83 -0.1971948546703608 2314 84-85 -1.2753540943082164 2314 86-87 -0.08718214432817817 2314 88-89 0.9545002245469476 2314 90-91 2.149089149635987 2314 92-93 3.262109451923507 2314 94-95 2.8856672288518013 2314 96-97 1.8430582338347463 2314 98-99 1.8664973183686744 2314 100-101 1.8752741315366581 2315 1 -2.9337295704505593 2315 2 -2.0143901961219015 2315 3 -2.847986863849556 2315 4 -3.923475385851731 2315 5 -3.935689127251049 2315 6 -2.991903562472775 2315 7 -5.725276925660054 2315 8 -3.1548616986232716 2315 9 -2.732481723135706 2315 10-11 -3.850447162671177 2315 12-13 -3.0285771646300716 2315 14-15 -2.068001011774662 2315 16-17 -2.5633984487545547 2315 18-19 -2.2531331301932482 2315 20-21 -1.5655531321601188 2315 22-23 -0.9973630204913917 2315 24-25 -2.3734717074195757 2315 26-27 -4.037213994637288 2315 28-29 -3.1596455862426254 2315 30-31 -3.341373121661931 2315 32-33 -3.799714993837817 2315 34-35 -3.6773261776037742 2315 36-37 -2.9568704736186184 2315 38-39 -1.8777213711578753 2315 40-41 -1.6142265320597353 2315 42-43 -3.034881857524965 2315 44-45 -6.576973025578123 2315 46-47 -3.265996674617597 2315 48-49 -2.239372724594066 2315 50-51 -3.182551632447428 2315 52-53 -3.272525282628248 2315 54-55 -1.7417156537606644 2315 56-57 -1.3387181970360764 2315 58-59 -2.7735728088950147 2315 60-61 -3.2070757697901904 2315 62-63 -4.792330902124252 2315 64-65 -4.28014476661102 2315 66-67 -3.7708912039643785 2315 68-69 -2.2012799370507103 2315 70-71 -3.7656502708602844 2315 72-73 -2.719057897746655 2315 74-75 -1.8208427773744091 2315 76-77 -3.5352448888755283 2315 78-79 -2.1059629653629344 2315 80-81 -1.716245730008314 2315 82-83 -1.4735317975338411 2315 84-85 -2.8702520912037777 2315 86-87 -2.2684021162403667 2315 88-89 -1.6240902108141313 2315 90-91 -0.8240518807431947 2315 92-93 -0.15345341303325455 2315 94-95 -0.1774573553746741 2315 96-97 -0.5687820721745656 2315 98-99 -0.7081552797538766 2315 100-101 -0.9870891251711544 2316 1 -7.54502728816772 2316 2 -6.165210202990096 2316 3 -3.681068827410993 2316 4 -4.317969227679043 2316 5 -2.117232707808487 2316 6 -3.0289700076246646 2316 7 -3.4096592057153714 2316 8 -2.6644664989351234 2316 9 -6.979436049634874 2316 10-11 -6.964343193039738 2316 12-13 -6.185671128971144 2316 14-15 -5.003233463070799 2316 16-17 -4.878864186667926 2316 18-19 -4.131114994444292 2316 20-21 -2.8318084805354147 2316 22-23 -3.7451642697604832 2316 24-25 -3.4262633040894315 2316 26-27 -4.421574231497221 2316 28-29 -4.640283075453063 2316 30-31 -7.306822849254441 2316 32-33 -5.317500604039871 2316 34-35 -5.956746650395509 2316 36-37 -4.7238715780987235 2316 38-39 -6.671276868962835 2316 40-41 -4.116237103512169 2316 42-43 -4.841969556957878 2316 44-45 -3.3652096163543206 2316 46-47 -4.410217412855815 2316 48-49 -5.667701943197596 2316 50-51 -5.180868692488449 2316 52-53 -6.444364520444161 2316 54-55 -5.21090297915535 2316 56-57 -4.595022086847944 2316 58-59 -3.3060087908149534 2316 60-61 -3.51096604572459 2316 62-63 -4.452910243705606 2316 64-65 -3.236939639595807 2316 66-67 -4.288061925032103 2316 68-69 -3.196969680235881 2316 70-71 -2.745434804426271 2316 72-73 -2.3709276247822864 2316 74-75 -2.4869171395139453 2316 76-77 -3.1426829590981846 2316 78-79 -5.133265899196225 2316 80-81 -5.94947758729835 2316 82-83 -3.6855729510194486 2316 84-85 -2.3597616185048516 2316 86-87 -2.4627704278708187 2316 88-89 -1.645893483416529 2316 90-91 -1.4181150681428427 2316 92-93 -1.3452835087239254 2316 94-95 -1.8701789242933042 2316 96-97 -3.3766542773294255 2316 98-99 -4.568137134723575 2316 100-101 -4.06790319197828 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23841.0 3 7829.0 4 7371.0 5 8163.0 6 9612.0 7 11531.0 8 14093.0 9 17343.0 10 20760.0 11 25183.0 12 30447.0 13 35015.0 14 38771.0 15 44529.0 16 49329.0 17 52677.0 18 46009.0 19 42090.0 20 43776.0 21 47544.0 22 52716.0 23 58767.0 24 65993.0 25 74009.0 26 83283.0 27 94209.0 28 106117.0 29 119746.0 30 135316.0 31 152315.0 32 170962.0 33 190844.0 34 217661.0 35 249106.0 36 264848.0 37 211664.0 38 62977.0 39 1107.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.844311728177786 20.10553185704789 13.639555581602531 21.41060083317179 2 15.627215859424926 20.980853587624857 40.42126337403898 22.970667178911242 3 19.077094961218805 26.89359999334228 30.009757714628112 24.0195473308108 4 12.205239818794253 16.888838950843276 38.00732632744168 32.898594902920784 5 12.57159956878153 39.00512531376553 33.92651879160351 14.496756325849425 6 29.97428817360026 37.14814437187056 18.219606880254467 14.657960574274718 7 27.422901719878706 34.535540471111545 21.03700599496216 17.004551814047588 8 26.721086472201538 34.011152892159224 20.335731398232202 18.93202923740704 9 27.954287569678186 16.194548406936928 20.417196693258475 35.43396733012641 10-11 24.54489765053412 27.32678013700246 27.292300584001765 20.83602162846166 12-13 26.236775635172716 25.156212470811234 27.821002732233328 20.786009161782722 14-15 23.114051708065784 25.84602043869043 27.020018123403904 24.01990972983988 16-17 21.068835333147195 29.340729089531198 27.705124714508433 21.885310862813174 18-19 21.116108076681652 27.13827545542892 31.23972443668796 20.505892031201462 20-21 22.226998029025687 26.19878098976804 31.22468742893747 20.349533552268806 22-23 21.71290621698869 26.397094241092066 31.11978534058304 20.770214201336206 24-25 21.13356410151833 26.68526002205864 31.563724907430952 20.61745096899208 26-27 20.798023885971098 26.84503904217277 31.383949818840012 20.97298725301612 28-29 20.760282232449512 27.181431476430063 31.379701646726232 20.678584644394196 30-31 20.938411190348134 27.005224020602437 31.199799654184794 20.85656513486463 32-33 20.61110196099598 27.01160845891794 31.06081498195306 21.316474598133016 34-35 21.260823329663666 27.08930001846404 30.915226448281942 20.734650203590355 36-37 21.387025221937815 26.621517516136077 30.874611924464173 21.116845337461932 38-39 20.906015066297677 27.062193421387814 30.957923896664052 21.073867615650457 40-41 21.134400517829455 26.883522212089687 31.1395979012636 20.84247936881726 42-43 20.812689920554384 26.96453682636767 30.73344906138121 21.48932419169673 44-45 20.919821837873855 27.16519557405579 30.70635001882605 21.208632569244305 46-47 21.33154196938739 27.393221855519396 29.81074755894269 21.464488616150526 48-49 20.95324516916746 27.649564540637982 29.590282625425786 21.806907664768772 50-51 21.229424552380827 27.765652305099426 29.53142474612605 21.4734983963937 52-53 21.258784600213886 27.767270593463977 29.331207205455478 21.642737600866653 54-55 20.865779552107202 27.868364370306235 29.510155337348376 21.755700740238186 56-57 21.03442416698327 28.160506685637987 28.98753991674996 21.81752923062878 58-59 20.932826695968462 27.56018285737295 29.228524681041833 22.278465765616755 60-61 20.681385904220157 27.798801459292193 29.496080572695494 22.023732063792153 62-63 20.945912301481894 27.548605782084607 29.617980873279272 21.887501043154227 64-65 20.748406134849684 27.648429071656178 29.467845269205583 22.13531952428855 66-67 20.642803960772614 27.89380379263741 29.073752012547153 22.389640234042815 68-69 21.147507589459845 27.585569684914223 29.184006401936685 22.082916323689247 70-71 20.924318653406274 27.69660759135203 28.879721863511694 22.499351891729997 72-73 20.814066979649628 27.844432413611024 28.69478745723707 22.646713149502276 74-75 20.912632010165044 28.189646792526197 28.542455024544687 22.355266172764072 76-77 21.03787369001463 27.90189840673279 28.68339309040227 22.376834812850305 78-79 21.09705447113879 28.044258277681216 28.504208543193382 22.354478707986615 80-81 21.215967407331267 28.14182269749339 28.22631166253309 22.41589823264226 82-83 21.309613311085684 28.104614882427192 28.135434011469922 22.450337795017198 84-85 21.288858878198063 28.019236544226523 28.09457186451446 22.597332713060958 86-87 21.30418125610057 28.06304336618274 28.05842589823205 22.574349479484642 88-89 21.44327867452313 27.91602429154436 27.907986826890546 22.732710207041965 90-91 21.238352897636283 27.996194039299276 28.1475350647869 22.61791799827754 92-93 21.65071107827452 27.57751287889951 28.156519079651147 22.61525696317483 94-95 21.332713641615484 27.49745897438125 28.2391604625101 22.930666921493167 96-97 21.850310460311352 27.31986485794194 27.67289786980204 23.15692681194467 98-99 21.841960163974694 27.444950037331722 27.329411041226802 23.38367875746678 100-101 22.095996452658557 27.493216682512937 27.50541414377297 22.905372721055528 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5505.0 1 3501.0 2 1563.5 3 1673.0 4 1509.0 5 1482.0 6 2222.5 7 3892.5 8 5907.5 9 6749.0 10 5977.0 11 5028.0 12 4877.0 13 5063.0 14 5246.5 15 5359.5 16 5623.0 17 6072.0 18 6658.5 19 7336.0 20 8158.0 21 9334.5 22 10961.0 23 12998.5 24 15119.5 25 17968.5 26 22395.0 27 27140.5 28 30599.0 29 34473.5 30 39378.0 31 45631.0 32 53982.0 33 59636.0 34 63501.0 35 69349.5 36 77498.0 37 84757.0 38 88298.0 39 90167.5 40 91324.5 41 92235.5 42 92683.0 43 94248.0 44 96373.0 45 98389.5 46 99061.5 47 99259.0 48 102581.0 49 101804.5 50 96767.0 51 93408.5 52 89806.5 53 87095.0 54 84468.0 55 78763.0 56 72123.0 57 65910.5 58 59771.5 59 52591.5 60 43980.0 61 36206.5 62 29242.5 63 21586.0 64 14884.0 65 10917.5 66 8256.0 67 6232.5 68 4679.5 69 3453.0 70 2512.5 71 1765.5 72 1334.0 73 1066.5 74 849.0 75 632.0 76 440.0 77 303.0 78 198.0 79 138.5 80 102.5 81 77.0 82 58.0 83 39.5 84 20.0 85 15.5 86 13.5 87 7.0 88 6.0 89 6.5 90 5.0 91 4.5 92 3.0 93 3.0 94 3.5 95 3.0 96 2.5 97 1.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13416203962316883 2 0.12169473599272464 3 0.12747817962129182 4 0.13783296791435518 5 0.12578124107159244 6 0.14043032283736437 7 0.17197952730218286 8 0.15701876294564981 9 0.16747744543563356 10-11 0.1622481041906417 12-13 0.15170284320322433 14-15 0.1572438670389773 16-17 0.15126995071605612 18-19 0.16635192496899623 20-21 0.1720661057996165 22-23 0.20657629487666548 24-25 0.22841139192942952 26-27 0.24068822286551972 28-29 0.23573593281231547 30-31 0.22629887659204867 32-33 0.22834212913148258 34-35 0.21738129135638376 36-37 0.2254330916177123 38-39 0.2131562606816221 40-41 0.2180219722373927 42-43 0.21073206275348022 44-45 0.2046023051351785 46-47 0.21893970431018928 48-49 0.25743250426918574 50-51 0.2591121271193983 52-53 0.26018570048757544 54-55 0.2585753404353098 56-57 0.23249789700829732 58-59 0.23301736799289915 60-61 0.20370188876186862 62-63 0.19630808508103573 64-65 0.19816086492611562 66-67 0.20583171979873618 68-69 0.20519103891772722 70-71 0.21090521974834747 72-73 0.24212542592291814 74-75 0.2456405129187239 76-77 0.26177874484035446 78-79 0.25831560494300887 80-81 0.273137843703648 82-83 0.27178721914368326 84-85 0.24581366991359122 86-87 0.24887854872274207 88-89 0.25251484561495496 90-91 0.4359746816768385 92-93 0.5808378235828052 94-95 0.3386258191624535 96-97 0.2895358111175795 98-99 0.32221053604903527 100-101 0.3430240068320824 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2887553.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.977500668822984 #Duplication Level Percentage of deduplicated Percentage of total 1 74.06581501966866 35.53492689643073 2 12.586677832036711 12.077546882096014 3 5.012832406814304 7.215095104518925 4 2.6329603447245336 5.052914268000228 5 1.5014943564251921 3.60189732448118 6 0.9318253779855696 2.682399161731733 7 0.6719964101667883 2.256849575275661 8 0.45304771712044434 1.7388877720923877 9 0.33188054564258085 1.433051919048263 >10 1.6189152256731874 13.779226646271455 >50 0.11509819383063516 3.857226699815476 >100 0.07105371684669923 6.279032060156417 >500 0.0041692996700053485 1.366901187689595 >1k 0.0020101980551568755 1.669343301144446 >5k 7.445177982062502E-5 0.33091103568712604 >10k+ 1.4890355964125004E-4 1.1237901655605216 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18339 0.6351052257742109 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13122 0.45443321732969055 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9264 0.32082528009009703 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3756 0.130075534544301 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2957 0.10240504676450961 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.7315699486728037E-5 0.0 16-17 0.0 0.0 0.0 1.0389419692036821E-4 0.0 18-19 0.0 0.0 0.0 2.424197928141925E-4 0.0 20-21 0.0 0.0 0.0 4.328924871682009E-4 0.0 22-23 0.0 0.0 0.0 0.0012294146635576904 0.0 24-25 0.0 0.0 0.0 0.002943668912743766 0.0 26-27 0.0 0.0 0.0 0.005956600623434444 0.0 28-29 0.0 0.0 0.0 0.017038648294940388 0.0 30-31 0.0 0.0 0.0 0.05061378959970605 0.0 32-33 0.0 0.0 0.0 0.11662123604311332 0.0 34-35 0.0 0.0 0.0 0.21062816855655983 0.0 36-37 0.0 0.0 0.0 0.3615171738839079 0.0 38-39 0.0 0.0 0.0 0.5966643729136747 0.0 40-41 0.0 1.7315699486728037E-5 0.0 0.8486770632435146 0.0 42-43 0.0 3.4631398973456074E-5 0.0 1.0924821120166452 0.0 44-45 0.0 3.4631398973456074E-5 0.0 1.3833858633936762 0.0 46-47 0.0 3.4631398973456074E-5 0.0 1.720713005094625 0.0 48-49 0.0 3.4631398973456074E-5 0.0 2.0788882489775946 0.0 50-51 0.0 3.4631398973456074E-5 0.0 2.4786904344266585 0.0 52-53 0.0 3.4631398973456074E-5 0.0 2.841662127067451 0.0 54-55 0.0 3.4631398973456074E-5 0.0 3.2168933349448476 0.0 56-57 0.0 3.4631398973456074E-5 0.0 3.638201619156428 0.0 58-59 0.0 3.4631398973456074E-5 0.0 4.111318476232298 0.0 60-61 0.0 3.4631398973456074E-5 0.0 4.630425831144917 0.0 62-63 0.0 3.4631398973456074E-5 1.7315699486728037E-5 5.112927797342595 0.0 64-65 0.0 3.4631398973456074E-5 3.4631398973456074E-5 5.575308920736693 0.0 66-67 0.0 3.4631398973456074E-5 3.4631398973456074E-5 6.097117524769242 0.0 68-69 1.7315699486728037E-5 3.4631398973456074E-5 3.4631398973456074E-5 6.6697130753963645 0.0 70-71 3.4631398973456074E-5 3.4631398973456074E-5 3.4631398973456074E-5 7.279727852614307 0.0 72-73 3.4631398973456074E-5 3.4631398973456074E-5 3.4631398973456074E-5 7.888184216878443 0.0 74-75 3.4631398973456074E-5 3.4631398973456074E-5 3.4631398973456074E-5 8.459602299940467 0.0 76-77 3.4631398973456074E-5 3.4631398973456074E-5 3.4631398973456074E-5 9.051020015909664 0.0 78-79 3.4631398973456074E-5 3.4631398973456074E-5 3.4631398973456074E-5 9.71092132334887 0.0 80-81 3.4631398973456074E-5 3.4631398973456074E-5 3.4631398973456074E-5 10.388242224471725 0.0 82-83 3.4631398973456074E-5 3.4631398973456074E-5 3.4631398973456074E-5 11.028334371698111 0.0 84-85 3.4631398973456074E-5 3.4631398973456074E-5 3.4631398973456074E-5 11.626210843575858 0.0 86-87 3.4631398973456074E-5 3.4631398973456074E-5 3.4631398973456074E-5 12.23262395530056 3.4631398973456074E-5 88-89 3.4631398973456074E-5 3.4631398973456074E-5 3.4631398973456074E-5 12.865079879053301 3.4631398973456074E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10895 0.0 41.3659 1 GTATCAA 18635 0.0 38.593655 1 TATCAAC 23155 0.0 31.613111 2 ATCAACG 23645 0.0 30.860344 3 TCAACGC 23745 0.0 30.750362 4 CAACGCA 24105 0.0 30.25175 5 AACGCAG 24780 0.0 29.657457 6 TACATGG 18565 0.0 28.594416 2 GTACATG 18385 0.0 28.306692 1 ACATGGG 19120 0.0 26.972761 3 ACGCAGA 27805 0.0 26.174973 7 CGCAGAG 28000 0.0 25.924456 8 CATGGGA 13010 0.0 24.579123 4 GCAGAGT 28385 0.0 24.185125 9 GAGTACT 15650 0.0 23.720284 12-13 ATGGGAT 5235 0.0 22.29473 5 CATGGGG 5855 0.0 22.04072 4 GTACTTT 16835 0.0 21.897545 14-15 AGTACTT 16335 0.0 21.360598 12-13 ATGGGAG 4850 0.0 20.934174 5 >>END_MODULE