FastQCFastQC Report
Fri 27 May 2016
SRR939753_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939753_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2887553
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203260.7039178155344681No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160440.5556261651301292No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119370.4133950095461451No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46490.16100137382759727No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36560.1266123946469554No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35810.1240150397239462No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117500.040.1481631
GTATCAA191600.035.4790531
ATCAACG244850.027.6407623
TATCAAC246550.027.5467722
TCAACGC245950.027.5152324
GTACATG189650.027.1129761
TACATGG187450.027.098392
CAACGCA250000.027.0883485
AACGCAG256000.026.4747126
ACATGGG191250.025.5233173
CATGGGA132600.023.4018654
ACGCAGA287600.023.3855427
CGCAGAG288600.023.2391858
GCAGAGT300550.021.8604159
GAGTACT165250.021.51939812-13
AGTACTT171300.021.41971212-13
CATGGGG54350.021.171834
ATGGGAT50300.020.9076395
GTACTTT173800.020.25737614-15
AGAGTAC280550.019.79847110-11
Produced by FastQC (version 0.11.5)