##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939753_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2887553 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.416813474938817 31.0 31.0 34.0 30.0 34.0 2 31.606583151893663 31.0 31.0 34.0 30.0 34.0 3 31.653843929444758 31.0 31.0 34.0 30.0 34.0 4 35.474809986171685 37.0 35.0 37.0 33.0 37.0 5 35.214388792171086 37.0 35.0 37.0 32.0 37.0 6 35.25606317875378 37.0 35.0 37.0 32.0 37.0 7 35.16689529161889 37.0 35.0 37.0 32.0 37.0 8 35.110437799756404 37.0 35.0 37.0 32.0 37.0 9 36.690497802118266 39.0 35.0 39.0 32.0 39.0 10-11 36.55227453833748 38.5 35.0 39.0 32.0 39.0 12-13 36.493573624449496 38.0 35.0 39.0 32.0 39.0 14-15 37.470987026039005 39.0 36.0 41.0 32.0 41.0 16-17 37.42176472605005 39.0 36.0 41.0 32.0 41.0 18-19 37.46487718147512 39.0 36.0 41.0 32.0 41.0 20-21 37.422636398362215 39.0 36.0 41.0 32.0 41.0 22-23 37.27692755769331 39.0 36.0 40.5 31.0 41.0 24-25 37.102347904956204 39.0 36.0 40.0 31.0 41.0 26-27 36.793729327219275 39.0 35.5 40.0 30.5 41.0 28-29 36.557401543798505 38.5 35.0 40.0 30.0 41.0 30-31 36.24471637403711 38.0 35.0 40.0 30.0 41.0 32-33 36.1102521754579 38.0 35.0 40.0 30.0 41.0 34-35 36.09680688112045 38.0 35.0 40.0 30.0 41.0 36-37 36.0073138051492 38.0 35.0 40.0 30.0 41.0 38-39 35.831075827872255 38.0 35.0 40.0 29.0 41.0 40-41 35.60875367482433 38.0 34.5 40.0 28.0 41.0 42-43 35.340544571822576 38.0 34.0 40.0 27.0 41.0 44-45 34.99968537374032 38.0 34.0 40.0 26.0 41.0 46-47 34.57157115384548 38.0 33.0 40.0 24.5 41.0 48-49 34.16467022423485 38.0 33.0 40.0 23.5 41.0 50-51 33.86300736298173 38.0 33.0 40.0 22.5 41.0 52-53 33.55955769469859 37.0 33.0 40.0 21.5 41.0 54-55 33.24354185014093 37.0 32.5 40.0 19.5 41.0 56-57 32.04641594457314 36.5 30.5 39.5 12.5 41.0 58-59 28.89833745042948 34.0 23.0 38.5 3.5 40.0 60-61 28.82886721040272 34.0 23.5 38.0 2.0 40.0 62-63 30.58866400027982 35.0 28.5 38.0 2.0 40.0 64-65 30.16102526949289 35.0 29.0 38.0 2.0 40.0 66-67 24.92184299301173 28.5 17.0 36.0 2.0 39.0 68-69 17.365295632668907 13.0 7.0 29.0 2.0 35.5 70-71 16.24104683100189 13.0 7.5 26.0 2.0 33.5 72-73 18.39491898503681 18.0 7.0 30.0 2.0 35.0 74-75 19.092330599646136 17.5 7.5 31.0 2.0 35.0 76-77 20.020029069596298 23.0 7.5 31.5 2.0 34.5 78-79 23.056176458059817 28.0 11.0 34.0 2.0 36.0 80-81 25.62057458339293 31.5 20.5 35.0 2.0 36.0 82-83 26.477495478005082 33.0 25.0 35.0 2.0 36.0 84-85 26.43981738170693 33.0 25.0 35.0 2.0 36.0 86-87 26.146900333950583 33.0 24.0 35.0 2.0 35.5 88-89 25.71191524449941 33.0 21.5 35.0 2.0 35.0 90-91 24.18812745601553 31.5 10.0 34.5 2.0 35.0 92-93 22.248037352041678 30.5 2.0 34.0 2.0 35.0 94-95 17.96321608642335 21.5 2.0 33.0 2.0 35.0 96-97 16.710740027975245 14.0 2.0 32.5 2.0 34.5 98-99 15.652642393057373 2.0 2.0 32.0 2.0 34.0 100-101 15.023507274152198 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.33443576735505687 1101 2 0.40085734622615377 1101 3 0.33700785147081547 1101 4 0.28087523765309186 1101 5 0.307110712347864 1101 6 0.39140070624886647 1101 7 0.4153107340127633 1101 8 0.427352748186145 1101 9 0.4791520629385957 1101 10-11 0.46672084299927263 1101 12-13 0.5105857599735444 1101 14-15 0.5772876857544418 1101 16-17 0.46969560043547176 1101 18-19 0.5227892277065891 1101 20-21 0.5253185681437671 1101 22-23 0.49562336845663424 1101 24-25 0.46867959103980894 1101 26-27 0.6251976218390496 1101 28-29 0.46356721808902535 1101 30-31 0.653359346716428 1101 32-33 0.5074672748717646 1101 34-35 0.633063650166811 1101 36-37 0.623823568276471 1101 38-39 0.5761061963494782 1101 40-41 0.6474402023724721 1101 42-43 0.5134521117728568 1101 44-45 0.18881122991748356 1101 46-47 0.111686684201878 1101 48-49 -0.06078672420430564 1101 50-51 0.006694839169057332 1101 52-53 0.037243118687548815 1101 54-55 0.12503365887266682 1101 56-57 0.7158269291792756 1101 58-59 -8.090314639189184 1101 60-61 -9.289527170168885 1101 62-63 -2.6955066791559403 1101 64-65 -0.3494373993341995 1101 66-67 -1.5451117632139102 1101 68-69 -3.739438223497242 1101 70-71 -4.21308888333534 1101 72-73 -6.450411303614619 1101 74-75 -7.604199584808947 1101 76-77 -8.77222882733869 1101 78-79 -11.64517774631723 1101 80-81 -7.791947757635242 1101 82-83 -3.7769233326660547 1101 84-85 -2.8126162879729577 1101 86-87 -2.717497447326462 1101 88-89 -2.905887226951151 1101 90-91 -8.747258111056194 1101 92-93 -14.221377873769546 1101 94-95 -12.2557588683268 1101 96-97 -12.199471622269545 1101 98-99 -11.727247800377338 1101 100-101 -11.534358187918407 1102 1 0.3945338148996207 1102 2 0.5045516697630603 1102 3 0.4447440126246036 1102 4 0.3141276476137662 1102 5 0.3375944035730569 1102 6 0.403242370958246 1102 7 0.41697024617375433 1102 8 0.43988304447763227 1102 9 0.4783926692142373 1102 10-11 0.5967132970363664 1102 12-13 0.6258455959612874 1102 14-15 0.6595604698149629 1102 16-17 0.7356857956810146 1102 18-19 0.8045809691249275 1102 20-21 0.7667025277253856 1102 22-23 0.6821336854370514 1102 24-25 0.6950472843239055 1102 26-27 0.7828453802882791 1102 28-29 0.7102931197316451 1102 30-31 0.744261414110369 1102 32-33 0.6830993117320432 1102 34-35 0.71161912298885 1102 36-37 0.6459531289615015 1102 38-39 0.6533556678822094 1102 40-41 0.6325825512158403 1102 42-43 0.8848488145368805 1102 44-45 0.7905779847036669 1102 46-47 0.6981932032142026 1102 48-49 0.7241483355387359 1102 50-51 0.7531665767894324 1102 52-53 0.7954257909578857 1102 54-55 0.6437886249658078 1102 56-57 1.3134492012637118 1102 58-59 -8.34705788155452 1102 60-61 -9.64198850335563 1102 62-63 -2.66670188971829 1102 64-65 -0.053624659150194987 1102 66-67 -1.1914898509369785 1102 68-69 -3.5554957327298027 1102 70-71 -4.0188830834382845 1102 72-73 -6.62587602998601 1102 74-75 -7.602862799988827 1102 76-77 -8.626795434204016 1102 78-79 -11.65401371907181 1102 80-81 -7.559528901723155 1102 82-83 -3.5607714739104885 1102 84-85 -2.5731954781062356 1102 86-87 -2.3980778368180857 1102 88-89 -2.450152145219878 1102 90-91 -7.9133346601024535 1102 92-93 -13.706434868277483 1102 94-95 -11.728990189569936 1102 96-97 -11.793160598667173 1102 98-99 -11.337277034736955 1102 100-101 -11.088725401459163 1103 1 0.299448935310199 1103 2 0.3130593681444296 1103 3 0.2766034678160736 1103 4 0.21937833849414545 1103 5 0.2028332507897943 1103 6 0.23454912184651988 1103 7 0.2849726149064864 1103 8 0.37936192327747165 1103 9 0.3211680343938639 1103 10-11 0.30073027295424737 1103 12-13 0.338399879419633 1103 14-15 0.5374720371264488 1103 16-17 0.3803956259218424 1103 18-19 0.37093509466776453 1103 20-21 0.3328888680333222 1103 22-23 0.37212578966129684 1103 24-25 0.4192577067479064 1103 26-27 0.1559010455073917 1103 28-29 0.27614388956978075 1103 30-31 0.30828628580400874 1103 32-33 0.32685177916197006 1103 34-35 0.2869843026177463 1103 36-37 0.10243753480437334 1103 38-39 0.12983612583720117 1103 40-41 0.20011117340297346 1103 42-43 0.3725116649119329 1103 44-45 0.23825860057417714 1103 46-47 0.3135603567870433 1103 48-49 0.2953682328936438 1103 50-51 0.20737107738161598 1103 52-53 0.259786233121325 1103 54-55 0.12193706555009953 1103 56-57 0.6761021819190667 1103 58-59 -6.477219744957598 1103 60-61 -8.351364737070593 1103 62-63 -2.462013772860807 1103 64-65 -0.20422671205189502 1103 66-67 -1.672895299022251 1103 68-69 -4.255219380578204 1103 70-71 -4.686303138708714 1103 72-73 -7.530949065911788 1103 74-75 -8.200306542586539 1103 76-77 -8.769383274709348 1103 78-79 -11.913005033718472 1103 80-81 -8.369280184786714 1103 82-83 -4.658619875015898 1103 84-85 -3.7287521302973126 1103 86-87 -3.5780126966680648 1103 88-89 -3.7382196541026254 1103 90-91 -8.829886180039288 1103 92-93 -14.487929143840057 1103 94-95 -12.382296545669423 1103 96-97 -12.315535253226983 1103 98-99 -11.859306249107266 1103 100-101 -11.588587225383364 1104 1 0.09905997361509478 1104 2 0.11489474667056498 1104 3 0.12044163504092253 1104 4 0.04911548599071125 1104 5 0.012804834302940549 1104 6 0.045036305746194216 1104 7 0.010625774916896091 1104 8 0.08399769363342813 1104 9 -0.003828987170010123 1104 10-11 0.10602626331735365 1104 12-13 0.010857067158191569 1104 14-15 0.09589902572896847 1104 16-17 0.18863883398434922 1104 18-19 0.194133030501213 1104 20-21 0.08921881698071843 1104 22-23 0.14734100924925997 1104 24-25 0.19555498114791448 1104 26-27 0.10917335399395256 1104 28-29 0.08798328346906459 1104 30-31 0.051978509658859195 1104 32-33 0.05441257235260366 1104 34-35 0.10178426202276825 1104 36-37 0.2147352769291686 1104 38-39 0.12953265629005273 1104 40-41 0.13608075364204097 1104 42-43 0.12203290888335516 1104 44-45 0.0815336227469814 1104 46-47 0.10961834142272409 1104 48-49 0.041916776989943116 1104 50-51 -0.10001676795150871 1104 52-53 0.08209968373886056 1104 54-55 -0.09454206995330594 1104 56-57 0.5467992242216759 1104 58-59 -6.789895527845051 1104 60-61 -8.59925644554568 1104 62-63 -2.733849791199148 1104 64-65 -0.6366992773458335 1104 66-67 -2.1060340411955814 1104 68-69 -4.253530717609211 1104 70-71 -4.519582529254498 1104 72-73 -7.55642616725363 1104 74-75 -8.47596532853185 1104 76-77 -8.97702097773941 1104 78-79 -12.025556494475921 1104 80-81 -8.40822136177642 1104 82-83 -4.686982088148373 1104 84-85 -3.7990583355931946 1104 86-87 -3.504933677654609 1104 88-89 -3.583193046739929 1104 90-91 -9.052384734946205 1104 92-93 -14.64129731384478 1104 94-95 -12.355088090440834 1104 96-97 -12.149600950909097 1104 98-99 -11.55650894602602 1104 100-101 -11.05070835727734 1105 1 0.12959558703261465 1105 2 0.15279869898405352 1105 3 0.07172858091075085 1105 4 0.08106523841717461 1105 5 0.0764397668436132 1105 6 0.08060937598882134 1105 7 0.09248907834032849 1105 8 0.18585141953915496 1105 9 0.14909645502953595 1105 10-11 0.1894627798524624 1105 12-13 0.10944134071537803 1105 14-15 0.23159088893979174 1105 16-17 0.2326021536789895 1105 18-19 0.3093036730283032 1105 20-21 0.2406229683848693 1105 22-23 0.20951252618783656 1105 24-25 0.1598139074870204 1105 26-27 0.26354534531068197 1105 28-29 0.23732079439920284 1105 30-31 0.3276585597159354 1105 32-33 0.21987378142161873 1105 34-35 0.29221051447502333 1105 36-37 0.3041868449831284 1105 38-39 0.27350001071244634 1105 40-41 0.21008196009178448 1105 42-43 0.21438055161019776 1105 44-45 0.2841765017170488 1105 46-47 0.2358100743316882 1105 48-49 0.2978779219001737 1105 50-51 0.27855545433685336 1105 52-53 0.31320326776768326 1105 54-55 0.2574866043938897 1105 56-57 0.9613638638675788 1105 58-59 -6.490186358959281 1105 60-61 -8.551204491480721 1105 62-63 -2.4640412621815173 1105 64-65 -0.20281589817534496 1105 66-67 -1.6460363826605473 1105 68-69 -3.8892857474595814 1105 70-71 -4.551389743347736 1105 72-73 -5.911555298384929 1105 74-75 -6.394623563278923 1105 76-77 -8.465404930370383 1105 78-79 -11.579032180941855 1105 80-81 -7.675947706696697 1105 82-83 -3.794210600577319 1105 84-85 -2.8816141570439235 1105 86-87 -2.741025111199999 1105 88-89 -2.8699877796343394 1105 90-91 -8.08527702623503 1105 92-93 -10.905651023214741 1105 94-95 -11.14392796730888 1105 96-97 -11.623644422785723 1105 98-99 -11.044794345470681 1105 100-101 -10.63837273936209 1106 1 0.12339880702032247 1106 2 0.18844171680158794 1106 3 0.11716211701888213 1106 4 0.08219220052369991 1106 5 0.10841245998633298 1106 6 -0.031181234115877032 1106 7 0.05412310061532821 1106 8 0.08324759402626114 1106 9 0.14257675810564052 1106 10-11 0.19772610493372156 1106 12-13 0.1999498900637775 1106 14-15 0.13445638464076382 1106 16-17 0.1139846053170217 1106 18-19 0.16646539684924733 1106 20-21 0.06067623176033976 1106 22-23 0.14488183721257997 1106 24-25 0.2382191189449685 1106 26-27 0.36073068327055324 1106 28-29 0.35852167805393975 1106 30-31 0.32506789049191553 1106 32-33 0.3320060631475812 1106 34-35 0.2748421417456015 1106 36-37 0.2261848877752115 1106 38-39 0.478681797034163 1106 40-41 0.43007666398570876 1106 42-43 0.4754824999725997 1106 44-45 0.47138175885812217 1106 46-47 0.31793929931989595 1106 48-49 0.31135460839644225 1106 50-51 0.3366827937769443 1106 52-53 0.36368338833528213 1106 54-55 0.23281033307650745 1106 56-57 1.0594502676547997 1106 58-59 -6.283980453521089 1106 60-61 -8.680802502473782 1106 62-63 -2.3401756421956037 1106 64-65 0.05781096819818998 1106 66-67 -1.2941141088955312 1106 68-69 -3.772462884852665 1106 70-71 -4.510647683867873 1106 72-73 -5.869683085698682 1106 74-75 -6.1314919074564 1106 76-77 -8.240259511390168 1106 78-79 -11.72350491607273 1106 80-81 -7.688769042054421 1106 82-83 -3.6597005351765652 1106 84-85 -2.6280039594084705 1106 86-87 -2.542460994166131 1106 88-89 -2.5386350556107296 1106 90-91 -7.760876589110335 1106 92-93 -11.37001589917053 1106 94-95 -11.46172450726089 1106 96-97 -12.070413910048757 1106 98-99 -11.588585472869465 1106 100-101 -11.307853676179432 1107 1 0.1617801895775557 1107 2 0.14686812041943753 1107 3 0.12561519796340903 1107 4 0.06373159968586606 1107 5 0.024927882320902484 1107 6 0.05713322302919721 1107 7 0.17245793301236034 1107 8 0.09170722825471955 1107 9 0.010562273163728264 1107 10-11 0.19579501599091742 1107 12-13 0.12101585815037907 1107 14-15 0.23675336526980573 1107 16-17 0.20547735055865957 1107 18-19 0.20370623494873286 1107 20-21 0.27220154682311914 1107 22-23 0.2593057243693977 1107 24-25 0.2796717526278698 1107 26-27 0.30582552294173126 1107 28-29 0.32793875798707717 1107 30-31 0.3819116214293743 1107 32-33 0.2873283946094105 1107 34-35 0.17012323120720652 1107 36-37 0.021552043699138324 1107 38-39 0.10378759161164908 1107 40-41 0.09460450260890951 1107 42-43 0.025420161859194934 1107 44-45 0.1633310495470468 1107 46-47 0.1621777340024977 1107 48-49 0.10380129333584875 1107 50-51 0.10554404601120382 1107 52-53 0.007463530028196885 1107 54-55 -0.13549312547370107 1107 56-57 0.5303016976086603 1107 58-59 -6.550137001644313 1107 60-61 -8.724034898851347 1107 62-63 -2.53169645733675 1107 64-65 -0.2587830389328758 1107 66-67 -1.4788025248193541 1107 68-69 -3.8497589234471405 1107 70-71 -4.60705806798315 1107 72-73 -5.872627093894655 1107 74-75 -6.530026593169094 1107 76-77 -8.953030059621518 1107 78-79 -12.141931658276917 1107 80-81 -8.039979624734201 1107 82-83 -4.04069852386451 1107 84-85 -3.0588570386054563 1107 86-87 -2.8208050666935094 1107 88-89 -2.8259374126631265 1107 90-91 -8.136283589000566 1107 92-93 -11.280338632020996 1107 94-95 -11.671671300056428 1107 96-97 -12.235911252419069 1107 98-99 -11.678600288752222 1107 100-101 -11.164470608882121 1108 1 -0.08360449256585412 1108 2 -0.024609338987364282 1108 3 -8.011026853331771E-4 1108 4 -0.04171761253468986 1108 5 -0.17795178392621125 1108 6 0.023731847079098145 1108 7 -0.04848276001581553 1108 8 -0.03522301240046488 1108 9 -0.12342806940645801 1108 10-11 -0.05279110978573698 1108 12-13 -0.13878132863405312 1108 14-15 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62-63 1.170182576787255 2311 64-65 1.0930467778809927 2311 66-67 -0.6741168573787739 2311 68-69 -3.325763864127067 2311 70-71 -0.9270769969506532 2311 72-73 -0.8122502199468187 2311 74-75 1.327283897747396 2311 76-77 3.9687305737110172 2311 78-79 4.680457480604659 2311 80-81 2.5566702571841446 2311 82-83 1.1943583129035638 2311 84-85 0.8125025758739106 2311 86-87 0.7622455289007668 2311 88-89 0.85312712933165 2311 90-91 2.335868239669338 2311 92-93 4.200181635338204 2311 94-95 8.175863545998606 2311 96-97 8.885918544448579 2311 98-99 9.346844512064772 2311 100-101 8.538071188432317 2312 1 -0.2592851734920707 2312 2 -0.24046613843791675 2312 3 -0.3123456964843925 2312 4 -0.106715895501722 2312 5 -0.19876958219936114 2312 6 -0.24814560582513678 2312 7 -0.26957896292005046 2312 8 -0.19738328516341852 2312 9 -0.23425150935936045 2312 10-11 -0.32611516296469745 2312 12-13 -0.3814796459417451 2312 14-15 -0.6274737553361192 2312 16-17 -0.6058734112490427 2312 18-19 -0.555318656300237 2312 20-21 -0.5038968347286854 2312 22-23 -0.3651995684956546 2312 24-25 -0.48446381816174977 2312 26-27 -0.5216282362427336 2312 28-29 -0.4792833174513831 2312 30-31 -0.45969658074219666 2312 32-33 -0.4473248228643598 2312 34-35 -0.6546580970337743 2312 36-37 -0.4760597386855565 2312 38-39 -0.4841684963233561 2312 40-41 -0.5898461139615065 2312 42-43 -0.5342414891278153 2312 44-45 -0.5035294011682083 2312 46-47 -0.4151456399217821 2312 48-49 -0.38245709299690844 2312 50-51 -0.37802696888839193 2312 52-53 -0.3221278495451614 2312 54-55 -0.3111103741117489 2312 56-57 0.8716125152308827 2312 58-59 4.073573201609253 2312 60-61 3.9273450912722048 2312 62-63 1.381135467265679 2312 64-65 1.1527523067271446 2312 66-67 -0.6552473979510083 2312 68-69 -1.0457084584047394 2312 70-71 -0.16609772279493207 2312 72-73 6.713673941638547 2312 74-75 5.39468026852601 2312 76-77 5.476901451994319 2312 78-79 5.352567735060568 2312 80-81 2.84782806088959 2312 82-83 1.544448457134333 2312 84-85 1.1494888270465147 2312 86-87 1.0047921400639943 2312 88-89 1.2445736877275273 2312 90-91 2.6023140651852827 2312 92-93 4.526338852739702 2312 94-95 8.346317491824124 2312 96-97 9.211361203923246 2312 98-99 9.737647601886538 2312 100-101 8.771656143873217 2313 1 -0.31079089000817817 2313 2 -0.32493328107284114 2313 3 -0.383284903229562 2313 4 -0.33798832803847745 2313 5 -0.3373288444245546 2313 6 -0.2632488603073213 2313 7 -0.3829354681396637 2313 8 -0.37996991148272485 2313 9 -0.4790187510863291 2313 10-11 -0.47088145049868757 2313 12-13 -0.42778672108462246 2313 14-15 -0.5584319523218824 2313 16-17 -0.5401867667820639 2313 18-19 -0.5806817357294136 2313 20-21 -0.5284075317720536 2313 22-23 -0.4918294381204831 2313 24-25 -0.4060545406694658 2313 26-27 -0.6710435139885078 2313 28-29 -0.7788034123953835 2313 30-31 -0.8000140065383832 2313 32-33 -0.5618545353534117 2313 34-35 -0.7156709062643145 2313 36-37 -0.7331396705309743 2313 38-39 -0.9496662285847322 2313 40-41 -0.9108275392449556 2313 42-43 -0.737706847961995 2313 44-45 -0.7461735635235982 2313 46-47 -0.6949448165072596 2313 48-49 -0.7466878550262166 2313 50-51 -0.532996099845441 2313 52-53 -0.6794474638639585 2313 54-55 -0.726216995274811 2313 56-57 0.18656056032352453 2313 58-59 3.6585521877594154 2313 60-61 3.4299221324530116 2313 62-63 0.9062518968123925 2313 64-65 0.8687395579888388 2313 66-67 1.44729603043195 2313 68-69 0.4038048311629403 2313 70-71 2.71522542599868 2313 72-73 7.921497441863231 2313 74-75 9.490780733884122 2313 76-77 8.377846409102442 2313 78-79 5.965623309419925 2313 80-81 3.1063409925448866 2313 82-83 1.6928341966663005 2313 84-85 1.3628036178298046 2313 86-87 1.1898301421495354 2313 88-89 1.2643487970456313 2313 90-91 2.6888330827914046 2313 92-93 4.550941638407078 2313 94-95 8.248141746761444 2313 96-97 8.919217200720041 2313 98-99 9.240314751290834 2313 100-101 8.485585308907554 2314 1 -0.34978491283405333 2314 2 -0.36812847045830566 2314 3 -0.3635396550108041 2314 4 -0.2533454425360162 2314 5 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66-67 1.6263092372436176 2314 68-69 -0.3263350513148424 2314 70-71 2.3935151276452924 2314 72-73 7.939224581698788 2314 74-75 9.284160183377722 2314 76-77 8.378442592517953 2314 78-79 6.128169058624053 2314 80-81 3.157667538994314 2314 82-83 1.7665721683252507 2314 84-85 1.4758033399056458 2314 86-87 1.3664109711576025 2314 88-89 1.5125232504012978 2314 90-91 2.7817597324698085 2314 92-93 4.665254732605323 2314 94-95 8.56516342845526 2314 96-97 9.105439922644287 2314 98-99 9.475984241715084 2314 100-101 8.657868058840782 2315 1 -0.6687645122131656 2315 2 -0.49848178555919986 2315 3 -0.46700628225597995 2315 4 -0.30422688240592777 2315 5 -0.5541457914962038 2315 6 -0.509857350751993 2315 7 -0.57699881013572 2315 8 -0.6352899480940408 2315 9 -0.8887837916859311 2315 10-11 -0.714592739356867 2315 12-13 -0.6559592427444798 2315 14-15 -0.7928229766669332 2315 16-17 -0.9057451078040231 2315 18-19 -0.8110851219090094 2315 20-21 -0.8996779372534576 2315 22-23 -0.8633911535593626 2315 24-25 -0.8925819605638239 2315 26-27 -0.8289632852045088 2315 28-29 -0.8232014392069331 2315 30-31 -0.8383281581371946 2315 32-33 -0.8230483731829068 2315 34-35 -0.9015620390465173 2315 36-37 -0.9155679825505914 2315 38-39 -1.0544280895866862 2315 40-41 -0.8877403521045011 2315 42-43 -0.9739844029433016 2315 44-45 -0.9134882187118549 2315 46-47 -0.8875709068519413 2315 48-49 -0.8238817773999259 2315 50-51 -0.6481339252947151 2315 52-53 -0.5837586674427584 2315 54-55 -0.8151115612873099 2315 56-57 0.6255030959883179 2315 58-59 3.8179330116611645 2315 60-61 3.474984750309485 2315 62-63 1.1350916744978647 2315 64-65 0.9973029128503157 2315 66-67 1.7772766036417238 2315 68-69 5.915477906036031 2315 70-71 5.20407854696675 2315 72-73 8.847428255288854 2315 74-75 9.745868365800664 2315 76-77 8.382420159226502 2315 78-79 5.866592075846313 2315 80-81 2.941591886691853 2315 82-83 1.3191665951121543 2315 84-85 0.9245181377473166 2315 86-87 0.8735447208804175 2315 88-89 1.0083886136949616 2315 90-91 2.274079933519406 2315 92-93 4.129239506054947 2315 94-95 8.10776912732436 2315 96-97 8.722987413220089 2315 98-99 8.900181336844065 2315 100-101 8.13473903969672 2316 1 -0.5420597610924425 2316 2 -0.5735711185089016 2316 3 -0.6392054042407054 2316 4 -0.4727761153027288 2316 5 -0.6217121299126163 2316 6 -0.5280385783027342 2316 7 -0.7538200421415127 2316 8 -0.7402605726357763 2316 9 -0.8259077811794455 2316 10-11 -0.7393439992994999 2316 12-13 -0.7002034163798925 2316 14-15 -0.755525658047631 2316 16-17 -0.9482040146471604 2316 18-19 -1.0602437679463392 2316 20-21 -0.9498640282292072 2316 22-23 -0.9773665394312943 2316 24-25 -2.193973466276489 2316 26-27 -1.4639971157085796 2316 28-29 -1.185792976476172 2316 30-31 -0.891163145096904 2316 32-33 -0.8405803706212609 2316 34-35 -0.8731543930741523 2316 36-37 -0.9926692791545477 2316 38-39 -1.3685974555779836 2316 40-41 -1.2221750717614626 2316 42-43 -1.3745032301524915 2316 44-45 -1.3259117636289304 2316 46-47 -1.1000079097198778 2316 48-49 -1.285302704977589 2316 50-51 -1.2439392212304128 2316 52-53 -1.1457933239865739 2316 54-55 -1.1797519555588636 2316 56-57 -0.2540250404103368 2316 58-59 3.190088279288762 2316 60-61 3.246638593250559 2316 62-63 0.7901131144057914 2316 64-65 0.42040116517297577 2316 66-67 5.4534010386398215 2316 68-69 8.321494770240669 2316 70-71 5.290371159943929 2316 72-73 8.737578891525082 2316 74-75 9.51961167163028 2316 76-77 8.130936304126866 2316 78-79 5.589005596910329 2316 80-81 2.546101228366023 2316 82-83 1.0971665579867746 2316 84-85 0.8489735548045765 2316 86-87 0.7596602921936331 2316 88-89 0.771526358142399 2316 90-91 2.039245651941947 2316 92-93 3.9348909172835214 2316 94-95 7.802409614409367 2316 96-97 8.306854810638015 2316 98-99 8.769091846111488 2316 100-101 7.734691008734151 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 21.0 4 72.0 5 192.0 6 446.0 7 1035.0 8 2382.0 9 3913.0 10 6947.0 11 10614.0 12 14667.0 13 19612.0 14 24591.0 15 33360.0 16 44514.0 17 55389.0 18 55214.0 19 50273.0 20 50600.0 21 49226.0 22 46907.0 23 45837.0 24 48351.0 25 53241.0 26 69224.0 27 97995.0 28 133959.0 29 173209.0 30 208400.0 31 226783.0 32 236730.0 33 247404.0 34 252952.0 35 245213.0 36 205653.0 37 128960.0 38 41724.0 39 1936.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.03387770993276 20.249451674626563 14.697648608992397 22.01902200644828 2 15.639123836412336 21.854737435608293 39.471484093334986 23.034654634644383 3 18.849023565478674 27.0077618821352 30.569631826625905 23.57358272576022 4 12.519735568489999 17.59039574338549 37.60166480061145 32.28820388751306 5 12.36102596376876 39.39454954581859 34.04615665593839 14.19826783447426 6 30.06694595735559 37.62687645906413 17.746063881771175 14.56011370180911 7 27.108281648856313 34.66613426662645 20.993554057709073 17.232030026808165 8 26.64562461407608 34.659924621244784 20.01464216562755 18.679808599051583 9 27.322753902698928 16.122024426910954 20.410014985006335 36.14520668538378 10-11 24.405491431672424 27.594333333448773 27.26751682133627 20.73265841354254 12-13 25.923749278368224 25.246220588851532 27.923747200484282 20.906282932295962 14-15 22.648401099064742 26.11369654370874 27.013024208099196 24.22487814912732 16-17 20.813955622632726 29.54136253083493 27.847575438442167 21.797106408090173 18-19 20.881515614375058 27.32979268855873 31.201044635085072 20.58764706198114 20-21 21.850282921213914 26.380675956424003 31.286802354796606 20.482238767565477 22-23 21.430013578971536 26.515980832213298 31.178821652797367 20.875183936017798 24-25 20.96725151018873 26.90821605698666 31.48645236987858 20.63808006294603 26-27 20.69208594425855 27.070079576221467 31.27652338698674 20.961311092533244 28-29 20.68474636730658 27.300417230520946 31.296877827388297 20.717958574784177 30-31 20.78136450977866 27.14661892669573 31.177241915306574 20.894774648219034 32-33 20.471962374886992 27.193169132404943 31.00426851303679 21.330599979671273 34-35 21.109620980652068 27.208181422277093 30.91439272662417 20.767804870446675 36-37 21.250388411363748 26.771095197070487 30.844296319852127 21.134220071713642 38-39 20.758290818093894 27.225520367132138 30.918110937755472 21.098077877018493 40-41 20.98147238177816 26.98984963858076 31.11834109322803 20.91033688641305 42-43 20.709796697160247 27.079222566171808 30.667991580779347 21.542989155888602 44-45 20.793224096719438 27.25539543423286 30.706775903280565 21.24460456576714 46-47 21.167869936363918 27.476379614629217 29.82773967803839 21.52801077096848 48-49 20.82917771607622 27.668044847756313 29.609184332731136 21.89359310343633 50-51 21.049827543623305 27.785654580806472 29.515795424510806 21.648722451059417 52-53 21.241308383665487 27.659374371623873 29.39542011333589 21.703897131374756 54-55 20.790341167493402 27.78459171398417 29.51046688721421 21.914600231308214 56-57 20.97351495983678 27.95159255022397 29.075704842132428 21.999187647806824 58-59 19.488875082372388 27.42227160930652 30.762006304355527 22.326847003965565 60-61 20.803686828692637 27.695872761889014 29.37024492877804 22.13019548064031 62-63 20.731501428291654 27.59699820660524 29.587706215722342 22.08379414938076 64-65 20.106120665438993 27.581541246605703 29.885989644565132 22.42634844339017 66-67 20.53718123601246 27.48959545703855 29.472978747281964 22.500244559667028 68-69 21.59806008421464 26.711267218380936 29.50039445025487 22.190278247149557 70-71 20.58971547122451 26.991281012326603 29.695008023309637 22.723995493139252 72-73 20.178618140972194 27.141871615146364 29.66096208822814 23.018548155653303 74-75 20.614222223785546 27.312540341089964 29.1088290487505 22.96440838637399 76-77 18.86215088039656 27.548985925072174 30.211941677427127 23.376921517104144 78-79 19.55223522473262 27.7179272826917 29.20654703367964 23.52329045889604 80-81 20.03937947969287 27.75209178594477 28.567040265526643 23.64148846883572 82-83 20.126345929773994 27.682565331803083 28.370172829997486 23.820915908425434 84-85 19.946702267750975 27.448851579321936 28.43401115650711 24.170434996419978 86-87 19.97794969573039 27.53733910974884 28.32262362257027 24.1620875719505 88-89 19.935911133059722 27.366753095094705 28.30661463183533 24.390721140010243 90-91 19.68721995117302 27.3409912695917 28.420179272898665 24.551609506336607 92-93 19.423286935598686 27.11448852637816 28.70774838930803 24.754476148715124 94-95 18.592071596226635 26.89376407571496 29.25642341925369 25.257740908804717 96-97 18.454004429484282 26.557092075655774 29.52021050833864 25.4686929865213 98-99 18.454985683689724 26.719483035439012 29.09805583929358 25.727475441577685 100-101 18.132326997842274 26.70159844070847 29.40497771695632 25.761096844492936 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1872.0 1 1372.0 2 877.0 3 979.5 4 1099.0 5 1242.0 6 1720.5 7 2606.5 8 3610.5 9 4231.5 10 4370.0 11 4520.0 12 4989.5 13 5419.5 14 5707.5 15 6053.0 16 6383.5 17 6622.5 18 6866.0 19 7527.5 20 8518.0 21 9632.5 22 11024.5 23 12865.5 24 15115.5 25 17910.0 26 21622.0 27 25933.5 28 30340.5 29 34619.0 30 39541.0 31 46135.5 32 53925.0 33 60920.5 34 66850.0 35 73352.5 36 80487.5 37 86491.0 38 90545.5 39 92922.5 40 94739.0 41 96592.0 42 97878.5 43 99387.0 44 101083.5 45 102761.0 46 103655.0 47 103735.5 48 104589.0 49 103473.0 50 100299.5 51 96637.5 52 92262.0 53 88589.0 54 84457.5 55 78368.5 56 70932.5 57 63161.0 58 54959.5 59 46283.5 60 37681.5 61 29869.0 62 22887.5 63 16491.0 64 11513.0 65 8262.0 66 6053.5 67 4375.5 68 3089.0 69 2136.5 70 1527.0 71 1085.5 72 831.0 73 708.0 74 509.0 75 317.0 76 189.0 77 118.0 78 64.5 79 38.0 80 24.0 81 19.0 82 16.0 83 7.5 84 4.0 85 4.0 86 2.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02012084280357798 2 0.005090815649098043 3 0.0037748224881067115 4 0.0 5 0.07037100271406274 6 0.0 7 0.0 8 6.926279794691215E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 2.424197928141925E-4 16-17 0.0 18-19 0.0012986774615046026 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.424197928141925E-4 28-29 0.002095199637894092 30-31 0.008484692748496737 32-33 4.67523886141657E-4 34-35 0.0012294146635576904 36-37 0.02749733078492412 38-39 0.025800392235224772 40-41 0.027999486070039235 42-43 0.028570904153101262 44-45 0.02954058332435803 46-47 0.03132410037149102 48-49 0.03109899627816355 50-51 0.02548870964446367 52-53 0.022423830835312804 54-55 0.017177173890834212 56-57 0.15816159911177388 58-59 0.753458031765997 60-61 0.3110419098800957 62-63 4.328924871682009E-4 64-65 0.1400147460496829 66-67 1.4060174826228298 68-69 2.6993963400844936 70-71 2.1807565090580154 72-73 1.8340269425357734 74-75 1.5448374454079283 76-77 1.114853995753498 78-79 0.41104007441595014 80-81 2.9436689127437663E-4 82-83 1.5584129538055233E-4 84-85 1.7315699486728037E-5 86-87 0.0022337252337879163 88-89 0.0 90-91 0.22022106607220718 92-93 0.17111374232784646 94-95 1.2701585044499615 96-97 1.2460204193654627 98-99 0.9835836779446127 100-101 0.39393216332306286 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2887553.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.501030921616874 #Duplication Level Percentage of deduplicated Percentage of total 1 68.34379310092854 27.679940776812927 2 14.488576347700178 11.736045573368235 3 6.275369473126659 7.624767992270201 4 3.259352442492264 5.280285362313066 5 1.9487740233138726 3.946367848873944 6 1.2709902056908777 3.088584817305506 7 0.8501171239783014 2.4101433947668838 8 0.5847681457443529 1.8946970202214881 9 0.4635571265271217 1.689708736386974 >10 2.2325142161604274 16.07559136382828 >50 0.16502997501937264 4.631771955854358 >100 0.10629316982334784 7.941963699559282 >500 0.0073571642247443935 2.0005404407122933 >1k 0.0032508400062622863 2.325850442568998 >5k 0.0 0.0 >10k+ 2.566452636522858E-4 1.673740575157618 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20326 0.7039178155344681 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16044 0.5556261651301292 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11937 0.4133950095461451 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4649 0.16100137382759727 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3656 0.1266123946469554 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3581 0.1240150397239462 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.7315699486728037E-5 0.0 16-17 0.0 0.0 0.0 1.0389419692036821E-4 0.0 18-19 0.0 0.0 0.0 2.2510409332746447E-4 0.0 20-21 0.0 0.0 0.0 3.982610881947448E-4 0.0 22-23 0.0 0.0 0.0 0.0011774675650975063 0.0 24-25 0.0 0.0 0.0 0.002943668912743766 0.0 26-27 0.0 0.0 0.0 0.006164389017275181 0.0 28-29 0.0 0.0 0.0 0.01755811927954223 0.0 30-31 0.0 0.0 0.0 0.052847514833493967 0.0 32-33 0.0 0.0 0.0 0.12166010459375118 0.0 34-35 0.0 0.0 0.0 0.21964964798914513 0.0 36-37 0.0 0.0 0.0 0.37729177611631715 0.0 38-39 0.0 0.0 0.0 0.6237807583098908 0.0 40-41 0.0 0.0 0.0 0.8870486533061038 0.0 42-43 0.0 0.0 0.0 1.142160853844068 0.0 44-45 0.0 0.0 0.0 1.4440081272967111 0.0 46-47 0.0 0.0 0.0 1.785075460086793 0.0 48-49 0.0 0.0 0.0 2.120549821942662 0.0 50-51 0.0 0.0 0.0 2.4750368218349585 0.0 52-53 0.0 1.7315699486728037E-5 0.0 2.7896111344103467 0.0 54-55 0.0 3.4631398973456074E-5 0.0 3.119405946834569 0.0 56-57 0.0 3.4631398973456074E-5 0.0 3.405790300645564 0.0 58-59 0.0 3.4631398973456074E-5 0.0 3.6104964999776628 3.4631398973456074E-5 60-61 0.0 6.926279794691215E-5 1.7315699486728037E-5 3.772969708261632 5.194709846018411E-5 62-63 1.0389419692036821E-4 6.926279794691215E-5 3.4631398973456074E-5 3.9069066437914737 8.657849743364017E-5 64-65 1.0389419692036821E-4 6.926279794691215E-5 3.4631398973456074E-5 4.038280855797279 1.0389419692036821E-4 66-67 1.0389419692036821E-4 6.926279794691215E-5 5.194709846018411E-5 4.14882428132055 1.0389419692036821E-4 68-69 1.0389419692036821E-4 6.926279794691215E-5 6.926279794691215E-5 4.275159624775719 1.0389419692036821E-4 70-71 1.0389419692036821E-4 6.926279794691215E-5 6.926279794691215E-5 4.509250566136794 1.0389419692036821E-4 72-73 1.0389419692036821E-4 6.926279794691215E-5 6.926279794691215E-5 4.799132691244109 1.385255958938243E-4 74-75 1.0389419692036821E-4 6.926279794691215E-5 6.926279794691215E-5 5.118191769986559 1.385255958938243E-4 76-77 1.0389419692036821E-4 6.926279794691215E-5 6.926279794691215E-5 5.5686077450353295 1.385255958938243E-4 78-79 1.0389419692036821E-4 6.926279794691215E-5 6.926279794691215E-5 6.2163361157353645 1.385255958938243E-4 80-81 1.0389419692036821E-4 8.657849743364017E-5 6.926279794691215E-5 6.930626035262383 1.385255958938243E-4 82-83 1.0389419692036821E-4 1.0389419692036821E-4 6.926279794691215E-5 7.602024967160776 1.385255958938243E-4 84-85 1.0389419692036821E-4 1.0389419692036821E-4 6.926279794691215E-5 8.13893286114575 1.385255958938243E-4 86-87 1.0389419692036821E-4 1.0389419692036821E-4 6.926279794691215E-5 8.6172963751661 1.385255958938243E-4 88-89 1.0389419692036821E-4 1.0389419692036821E-4 6.926279794691215E-5 9.09640446426438 1.385255958938243E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11750 0.0 40.148163 1 GTATCAA 19160 0.0 35.479053 1 ATCAACG 24485 0.0 27.640762 3 TATCAAC 24655 0.0 27.546772 2 TCAACGC 24595 0.0 27.515232 4 GTACATG 18965 0.0 27.112976 1 TACATGG 18745 0.0 27.09839 2 CAACGCA 25000 0.0 27.088348 5 AACGCAG 25600 0.0 26.474712 6 ACATGGG 19125 0.0 25.523317 3 CATGGGA 13260 0.0 23.401865 4 ACGCAGA 28760 0.0 23.385542 7 CGCAGAG 28860 0.0 23.239185 8 GCAGAGT 30055 0.0 21.860415 9 GAGTACT 16525 0.0 21.519398 12-13 AGTACTT 17130 0.0 21.419712 12-13 CATGGGG 5435 0.0 21.17183 4 ATGGGAT 5030 0.0 20.907639 5 GTACTTT 17380 0.0 20.257376 14-15 AGAGTAC 28055 0.0 19.798471 10-11 >>END_MODULE