FastQCFastQC Report
Fri 27 May 2016
SRR939752_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939752_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3058367
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197660.6462926130186469No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137290.44889969058651236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100970.33014350468730536No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39920.13052717348833545No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30930.1011324017032619No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA197000.038.3724141
GGTATCA116800.037.7771721
TATCAAC247850.030.4623012
ATCAACG253150.029.8993553
TCAACGC254000.029.7817524
CAACGCA257450.029.3836215
GTACATG202650.028.6907121
AACGCAG265500.028.5481996
TACATGG200300.028.3702132
ACATGGG205500.026.7371253
CATGGGA141400.025.284954
ACGCAGA295250.025.1616957
CGCAGAG298200.024.801938
ATGGGAT55050.023.2446885
GCAGAGT303950.022.7848559
AGTACTT164250.022.02569212-13
GAGTACT157800.021.85272412-13
GTATCGC1303.1392492E-421.714796
CATGGGG61750.020.7219144
ATGGGAG52900.020.6321495
Produced by FastQC (version 0.11.5)