##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939752_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3058367 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.092533041325648 31.0 30.0 33.0 26.0 34.0 2 30.13677920275755 31.0 30.0 33.0 26.0 34.0 3 30.05645627225248 31.0 30.0 34.0 25.0 34.0 4 33.72872385818968 35.0 33.0 37.0 28.0 37.0 5 33.62209407831042 35.0 33.0 37.0 28.0 37.0 6 33.618270142203336 35.0 33.0 37.0 28.0 37.0 7 33.62002990484791 35.0 33.0 37.0 28.0 37.0 8 33.46091819588689 35.0 33.0 37.0 27.0 37.0 9 34.739140528262304 37.0 34.0 39.0 27.0 39.0 10-11 34.5486766303717 37.0 34.0 39.0 27.0 39.0 12-13 34.46298351375097 37.0 33.0 39.0 27.0 39.0 14-15 35.011573987032946 38.0 33.0 40.0 26.0 41.0 16-17 34.84113875149712 38.0 33.0 40.0 25.0 41.0 18-19 34.94333086905529 38.0 33.5 40.0 25.5 41.0 20-21 35.07473449066119 38.0 34.0 40.0 25.5 41.0 22-23 34.99326209052086 38.0 34.0 40.0 25.5 41.0 24-25 34.806402403635666 38.0 33.0 40.0 25.0 41.0 26-27 34.64826376298201 38.0 33.0 40.0 24.0 41.0 28-29 34.50692477390712 38.0 33.0 40.0 24.0 41.0 30-31 34.25814315286557 38.0 33.0 40.0 23.5 41.0 32-33 33.91670129843802 38.0 33.0 40.0 23.0 41.0 34-35 33.60075082552225 37.0 32.0 40.0 20.5 41.0 36-37 33.332991593226055 37.0 31.5 40.0 18.5 41.0 38-39 33.02005367570341 37.0 31.0 40.0 17.0 41.0 40-41 32.67785815109828 37.0 31.0 40.0 15.5 41.0 42-43 32.297958027927976 37.0 30.5 40.0 13.5 41.0 44-45 31.80338379926281 36.0 30.0 39.0 9.0 41.0 46-47 31.310687533575923 36.0 30.0 39.0 7.5 40.5 48-49 30.887193394383345 36.0 29.0 39.0 3.5 40.0 50-51 30.118473028253312 34.5 27.5 38.5 2.0 40.0 52-53 30.204410065894642 35.0 28.0 38.5 2.0 40.0 54-55 30.279085701617888 35.0 28.0 39.0 2.0 40.0 56-57 30.062336861468882 35.0 28.0 39.0 2.0 40.0 58-59 29.54014838637744 35.0 26.5 38.5 2.0 40.0 60-61 29.05619289640517 34.0 26.0 38.0 2.0 40.0 62-63 28.622470913399212 34.0 26.0 38.0 2.0 40.0 64-65 28.101435504633685 34.0 25.0 38.0 2.0 40.0 66-67 27.56742912148869 33.0 23.0 37.0 2.0 39.0 68-69 27.03281506110941 33.0 22.0 36.0 2.0 39.0 70-71 26.50317211766933 32.0 21.0 36.0 2.0 39.0 72-73 26.034402182602676 32.0 20.0 35.5 2.0 38.0 74-75 25.440979614284355 31.5 18.0 35.0 2.0 38.0 76-77 24.786820711837397 31.0 14.0 35.0 2.0 37.0 78-79 24.12691560561568 31.0 7.5 34.0 2.0 36.0 80-81 23.44115258240754 30.0 4.5 34.0 2.0 36.0 82-83 22.68484946378247 29.5 2.0 34.0 2.0 35.5 84-85 21.935550573230746 29.0 2.0 34.0 2.0 35.0 86-87 21.150121944161704 28.5 2.0 34.0 2.0 35.0 88-89 20.297404791511287 27.0 2.0 33.0 2.0 35.0 90-91 19.37360820333204 26.0 2.0 32.5 2.0 34.0 92-93 18.414843280744265 25.0 2.0 32.0 2.0 34.0 94-95 17.07803576222213 20.5 2.0 32.0 2.0 34.0 96-97 14.582459201266559 2.0 2.0 30.5 2.0 34.0 98-99 13.229664883253058 2.0 2.0 29.5 2.0 34.0 100-101 11.973860396741136 2.0 2.0 27.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7564549633508939 1101 2 0.6724955363833409 1101 3 0.7843063855805852 1101 4 0.8344526214987553 1101 5 0.7711832216072096 1101 6 0.8707456249606551 1101 7 0.765906327166185 1101 8 0.5921735970057185 1101 9 0.733035111328384 1101 10-11 0.6980597691467096 1101 12-13 0.5383932101157711 1101 14-15 0.7618768237601117 1101 16-17 0.7540460697711353 1101 18-19 0.7287699913053487 1101 20-21 0.8051112800015332 1101 22-23 0.8882219852875579 1101 24-25 0.8652173470668956 1101 26-27 0.879216147942735 1101 28-29 1.1645760363444353 1101 30-31 1.0496628877346197 1101 32-33 0.9884852350442728 1101 34-35 0.8457648456762001 1101 36-37 0.8423020159235151 1101 38-39 0.8543980176247103 1101 40-41 0.9580516674188004 1101 42-43 1.0743961344481008 1101 44-45 0.9675986079736347 1101 46-47 0.7214629360375291 1101 48-49 0.7531423757247495 1101 50-51 0.8038001660156304 1101 52-53 0.7883581689922003 1101 54-55 0.6734776030244376 1101 56-57 0.3926393876927783 1101 58-59 0.16153830922335288 1101 60-61 0.45850083369775163 1101 62-63 0.41617119109516665 1101 64-65 0.4006450830977357 1101 66-67 0.36098046727730804 1101 68-69 0.3802989534798158 1101 70-71 0.38909013822479466 1101 72-73 0.2304278352589506 1101 74-75 -0.0825307437502083 1101 76-77 -0.5318319110240068 1101 78-79 -0.44854728446811976 1101 80-81 -0.48940517170419184 1101 82-83 -0.5692900828140743 1101 84-85 -0.45470938775197567 1101 86-87 -0.4479528137600397 1101 88-89 -0.6384464180229017 1101 90-91 -0.7113390986925161 1101 92-93 -0.6428041630663053 1101 94-95 -0.4157002072717866 1101 96-97 0.8639992424480702 1101 98-99 0.8867063900993841 1101 100-101 0.22790414612136267 1102 1 0.7506399251747666 1102 2 0.831728235857689 1102 3 0.8905361839490631 1102 4 0.9184581765101569 1102 5 0.9681077928545463 1102 6 0.8277790576405408 1102 7 0.8161082851794461 1102 8 0.7376542617414188 1102 9 0.8696262419667704 1102 10-11 0.8695543050863819 1102 12-13 0.7572415946848707 1102 14-15 0.8976173870037556 1102 16-17 0.9251319089230066 1102 18-19 0.8801685388867497 1102 20-21 0.8639165094547536 1102 22-23 0.7231474287323181 1102 24-25 0.7560739079038328 1102 26-27 0.9196638370859773 1102 28-29 1.0727467071901842 1102 30-31 1.015465286044325 1102 32-33 1.211558879756744 1102 34-35 1.1195076220625069 1102 36-37 0.9912844716956428 1102 38-39 0.9897349563516755 1102 40-41 1.09286487957732 1102 42-43 0.973268282898907 1102 44-45 1.020451264910875 1102 46-47 1.0271274385351923 1102 48-49 0.778304047078894 1102 50-51 0.9635218440861628 1102 52-53 1.106854467983343 1102 54-55 1.0830802284786074 1102 56-57 0.9263336102010662 1102 58-59 0.7893059618928824 1102 60-61 0.9231822600903534 1102 62-63 0.7302647587557658 1102 64-65 0.723645966955555 1102 66-67 0.5199421209076576 1102 68-69 0.4008719102290854 1102 70-71 0.4380936993089293 1102 72-73 0.2971386948165069 1102 74-75 0.2910301304994469 1102 76-77 0.23025227934824244 1102 78-79 0.057935369530696335 1102 80-81 -0.24244142153511916 1102 82-83 -0.29437537859161367 1102 84-85 -0.29525012683435037 1102 86-87 -0.3288446939285059 1102 88-89 -0.40082182671969235 1102 90-91 -0.4025504852998587 1102 92-93 -0.3294546451069671 1102 94-95 -0.1674782423555783 1102 96-97 0.8758202577418412 1102 98-99 0.8689207920844488 1102 100-101 0.5014232761627326 1103 1 0.6671392110658907 1103 2 0.5594613239975885 1103 3 0.7054327047290343 1103 4 0.6885696182849301 1103 5 0.8340242627942729 1103 6 0.8724214848289122 1103 7 0.7576586577567568 1103 8 0.7024509885677261 1103 9 0.9050287064780207 1103 10-11 0.8050139322994525 1103 12-13 0.680585731712199 1103 14-15 0.8353710571154735 1103 16-17 0.5952758694201208 1103 18-19 0.6917608968736886 1103 20-21 0.7590396642013815 1103 22-23 0.7948376714726848 1103 24-25 0.9280222042683661 1103 26-27 0.9579097636061249 1103 28-29 1.0013435243210935 1103 30-31 0.9384509806391392 1103 32-33 0.9296953756774613 1103 34-35 1.0454030058202477 1103 36-37 0.9798419037816544 1103 38-39 0.7364023255133887 1103 40-41 0.7441521734247658 1103 42-43 0.9256232559123347 1103 44-45 1.0408569331394517 1103 46-47 0.764119581354695 1103 48-49 0.6361703770011609 1103 50-51 0.8450689987829385 1103 52-53 0.9702610477559048 1103 54-55 0.9398717976315965 1103 56-57 0.5782632337940221 1103 58-59 0.3523096834170474 1103 60-61 0.743641181975736 1103 62-63 0.4259761964515363 1103 64-65 0.4910836785892698 1103 66-67 0.5090176997437474 1103 68-69 0.12043668055569157 1103 70-71 0.3886300032898653 1103 72-73 0.26974529702359007 1103 74-75 0.3039056750039215 1103 76-77 0.15825339200974753 1103 78-79 0.10958841402526076 1103 80-81 0.11214646597729683 1103 82-83 0.25404516893529205 1103 84-85 -0.2984167932177506 1103 86-87 -0.4482514028695128 1103 88-89 -0.1312859776609514 1103 90-91 -0.35474649048245865 1103 92-93 -0.4346385486793203 1103 94-95 -0.36723446524365855 1103 96-97 1.031583804707319 1103 98-99 1.164183426089627 1103 100-101 0.6653844871055661 1104 1 0.3521041699329963 1104 2 0.42590441003670776 1104 3 0.6008958001542197 1104 4 0.6983549970839817 1104 5 0.6597633614835203 1104 6 0.8223648065997793 1104 7 0.5745503361830089 1104 8 0.7270906538232396 1104 9 0.9113399447528607 1104 10-11 0.7882404167749115 1104 12-13 0.8311455743632763 1104 14-15 0.8834354375352191 1104 16-17 0.9067970223616868 1104 18-19 0.809461191902912 1104 20-21 0.7226602878401209 1104 22-23 0.6823270392417555 1104 24-25 0.8700335982395728 1104 26-27 1.1210488788209005 1104 28-29 1.1241452920121802 1104 30-31 1.055102768933125 1104 32-33 1.0845757789434671 1104 34-35 1.085243777529854 1104 36-37 0.897381479982954 1104 38-39 0.6944044867927914 1104 40-41 0.8386361045122541 1104 42-43 0.8017857806668687 1104 44-45 0.882260555037611 1104 46-47 0.8133276792938098 1104 48-49 0.6076180507635449 1104 50-51 0.8113323562402961 1104 52-53 0.9389720730350959 1104 54-55 0.7237948923763184 1104 56-57 0.9084965191169267 1104 58-59 0.6681982732983478 1104 60-61 0.9323990187737614 1104 62-63 0.8066162743051208 1104 64-65 0.8810797956310275 1104 66-67 0.8115624145191305 1104 68-69 0.4251672469977521 1104 70-71 0.7524400224022258 1104 72-73 0.7809690445265325 1104 74-75 0.6566271750895218 1104 76-77 0.2848403689242396 1104 78-79 0.37617288510856284 1104 80-81 0.3343158893282947 1104 82-83 0.4765858225359949 1104 84-85 0.4247592290020563 1104 86-87 0.2715062659730165 1104 88-89 0.5226729925936482 1104 90-91 0.12621092064829398 1104 92-93 0.27834468115345956 1104 94-95 0.5699708633199521 1104 96-97 1.7481497250791325 1104 98-99 1.9141603389596096 1104 100-101 1.8069261457389931 1105 1 0.6882409906591747 1105 2 0.8640963941605051 1105 3 0.9069686313728944 1105 4 0.8129820542001767 1105 5 0.6989563155222456 1105 6 0.8850425413229388 1105 7 0.8517527814944472 1105 8 0.8318235456191658 1105 9 0.9299809485731743 1105 10-11 0.9953685377826815 1105 12-13 0.7680318470153082 1105 14-15 0.8911880104556715 1105 16-17 0.932487856154367 1105 18-19 0.8450615287641412 1105 20-21 0.6746552966943753 1105 22-23 0.5127751892721335 1105 24-25 0.7212605495832847 1105 26-27 0.8325219045707115 1105 28-29 0.8710938970624227 1105 30-31 0.8981393099852113 1105 32-33 0.8129144773262809 1105 34-35 1.0392811314298882 1105 36-37 1.065804187060742 1105 38-39 1.0227595876060676 1105 40-41 0.9528356548368748 1105 42-43 1.047735692746251 1105 44-45 1.1253425717227614 1105 46-47 0.9821592348714354 1105 48-49 1.2302467215584336 1105 50-51 1.1211550824401968 1105 52-53 1.2315570149081907 1105 54-55 1.1142139544461287 1105 56-57 0.9935818080658727 1105 58-59 0.9535032662677558 1105 60-61 1.0722281046049567 1105 62-63 0.8916761584613724 1105 64-65 0.8073550872353543 1105 66-67 0.8447870870213094 1105 68-69 0.6929428803018141 1105 70-71 0.7993900821667381 1105 72-73 0.7465873471089992 1105 74-75 0.8502697785684603 1105 76-77 0.7353549424731725 1105 78-79 0.5486307376614654 1105 80-81 0.5395934134973714 1105 82-83 0.46695150291692755 1105 84-85 0.4931969997515253 1105 86-87 0.36159129191383954 1105 88-89 0.31469238794060317 1105 90-91 0.32058557218888595 1105 92-93 0.4832470366204866 1105 94-95 0.4513677453191214 1105 96-97 1.668007671176488 1105 98-99 1.9722560926802775 1105 100-101 1.1978482437869449 1106 1 0.9392592900966825 1106 2 0.98354890595499 1106 3 1.0204162068018512 1106 4 0.988392058894334 1106 5 1.0712966939754978 1106 6 1.140228159093418 1106 7 1.0804419440597997 1106 8 1.0487964225928792 1106 9 1.211188209112656 1106 10-11 1.1690677497710524 1106 12-13 1.1771814686279 1106 14-15 1.4425335408235327 1106 16-17 1.4769780728486595 1106 18-19 1.3786149768965146 1106 20-21 1.3834893876876109 1106 22-23 1.0501219019758565 1106 24-25 1.2613264512611622 1106 26-27 1.6380033707492032 1106 28-29 1.5429791830686455 1106 30-31 1.4505970714847365 1106 32-33 1.590383251041935 1106 34-35 1.542643729572461 1106 36-37 1.5119200453967423 1106 38-39 1.43142435869283 1106 40-41 1.6052811547558719 1106 42-43 1.4123115548535523 1106 44-45 1.5305433923419969 1106 46-47 1.521524736936204 1106 48-49 1.3165420738905276 1106 50-51 1.6843766443432067 1106 52-53 1.5924401012775355 1106 54-55 1.5003354427472786 1106 56-57 1.5614356245529706 1106 58-59 1.4830359737639753 1106 60-61 1.7581601320583786 1106 62-63 1.6404075004319978 1106 64-65 1.5334754162990478 1106 66-67 1.5620627797007671 1106 68-69 1.4816053652945982 1106 70-71 1.7087818052329027 1106 72-73 1.8211801217783865 1106 74-75 1.7209743801475064 1106 76-77 1.705025610020165 1106 78-79 1.3066874109888893 1106 80-81 1.3942301096663066 1106 82-83 1.5277966820056221 1106 84-85 1.660690189771163 1106 86-87 1.4022422631098088 1106 88-89 1.2347360892498003 1106 90-91 1.2326462398125138 1106 92-93 1.2794070571543124 1106 94-95 1.6676963496328447 1106 96-97 3.330646959115418 1106 98-99 3.2865650333477223 1106 100-101 2.762848058675937 1107 1 0.6630025928389074 1107 2 0.7262718680971574 1107 3 0.729236927737638 1107 4 0.354520413055063 1107 5 0.7204180452439246 1107 6 0.8095056446053022 1107 7 0.7586457023964002 1107 8 0.6931084715080189 1107 9 0.9087770473780594 1107 10-11 0.9069757181407994 1107 12-13 0.851643039102143 1107 14-15 0.9814800282924168 1107 16-17 1.0137289491144372 1107 18-19 1.0610012368107604 1107 20-21 0.9142762949981105 1107 22-23 0.811913511781519 1107 24-25 0.9653609546651651 1107 26-27 1.1687610762924123 1107 28-29 1.1477921449512465 1107 30-31 1.1123196502273842 1107 32-33 1.30122772359951 1107 34-35 1.177964080938338 1107 36-37 0.8951949964718153 1107 38-39 0.9966243519695084 1107 40-41 1.1532683285311833 1107 42-43 1.1430100909141743 1107 44-45 0.9982449079403111 1107 46-47 0.999260543600716 1107 48-49 0.8623926782873248 1107 50-51 1.0074591999201807 1107 52-53 1.1829111656771651 1107 54-55 1.174128989550315 1107 56-57 1.0104432219060264 1107 58-59 1.053357499583445 1107 60-61 1.1933702932946844 1107 62-63 1.1136467668410504 1107 64-65 1.1062606883105843 1107 66-67 1.282377560922619 1107 68-69 0.9560266383937801 1107 70-71 0.9076821698666961 1107 72-73 0.823628070293065 1107 74-75 0.8352923843083069 1107 76-77 0.8172062469399677 1107 78-79 0.8893127211446377 1107 80-81 0.7402652543389863 1107 82-83 0.9078526302513659 1107 84-85 0.7794398413712074 1107 86-87 0.8284065253380639 1107 88-89 0.7936410006360006 1107 90-91 0.7134195089934501 1107 92-93 0.9043118455633063 1107 94-95 1.1543493866804688 1107 96-97 2.5004012472539916 1107 98-99 2.7150240544917157 1107 100-101 2.1897825773563433 1108 1 0.46246992301507817 1108 2 0.5058896181605341 1108 3 0.5404858842763645 1108 4 0.6171380194154565 1108 5 0.6803442884765687 1108 6 0.6118333100130897 1108 7 0.6215118826257751 1108 8 0.5563775044838195 1108 9 0.7497692922608579 1108 10-11 0.559359411565822 1108 12-13 0.4608385365162917 1108 14-15 0.4303796913169933 1108 16-17 0.761180844944306 1108 18-19 0.6646278399467178 1108 20-21 0.6575547493998641 1108 22-23 0.3987619842510952 1108 24-25 0.6362807183464128 1108 26-27 0.7987103523684453 1108 28-29 0.7114273379734115 1108 30-31 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1.3700274089024482 2311 92-93 1.4090066978382758 2311 94-95 1.9086934206282535 2311 96-97 3.3020361094456643 2311 98-99 3.6529636601733078 2311 100-101 3.2966641324866455 2312 1 0.1173448755732629 2312 2 -0.2804852456355462 2312 3 -0.5521113561403901 2312 4 -0.03729312261615547 2312 5 -1.6641364685111526 2312 6 -0.35538401328911107 2312 7 0.021628497667585123 2312 8 0.17648334564250234 2312 9 0.21806553425696507 2312 10-11 0.19514132919459826 2312 12-13 0.3148137780104321 2312 14-15 0.5839683585386766 2312 16-17 -0.14454499951999367 2312 18-19 0.2776212348217726 2312 20-21 0.6525880887527009 2312 22-23 0.4252398733045837 2312 24-25 0.45362380071764363 2312 26-27 0.604593940867332 2312 28-29 0.7296865064867433 2312 30-31 0.3917919939987442 2312 32-33 -0.1251926144037938 2312 34-35 0.14403963288194888 2312 36-37 0.5057999046055244 2312 38-39 0.6753432896023668 2312 40-41 0.8206007098446548 2312 42-43 0.7862893383316063 2312 44-45 0.8522774216456668 2312 46-47 0.4578274791948651 2312 48-49 0.622357031929031 2312 50-51 0.4889723132426589 2312 52-53 0.36944192348082794 2312 54-55 0.25905538929920624 2312 56-57 0.1768240365822571 2312 58-59 0.5443169367004081 2312 60-61 0.6873660418371337 2312 62-63 0.733593305073093 2312 64-65 0.5163423998951835 2312 66-67 0.648222786734685 2312 68-69 0.6874620569510199 2312 70-71 0.9206385489177009 2312 72-73 0.8179332382380728 2312 74-75 1.0587056241178594 2312 76-77 0.8428060893749816 2312 78-79 0.9206948022455386 2312 80-81 0.992819858219999 2312 82-83 1.1240031576434326 2312 84-85 1.2829543640767263 2312 86-87 1.2412520339590714 2312 88-89 1.1528369142021262 2312 90-91 1.1769385146869205 2312 92-93 1.1456405270520627 2312 94-95 1.7811336469641503 2312 96-97 3.2475941353859614 2312 98-99 3.4916758840480444 2312 100-101 3.1243464921780184 2313 1 -0.02809972707671804 2313 2 -0.44846669870014466 2313 3 -0.8508114476208988 2313 4 -0.05237025396446171 2313 5 -0.34465868204436845 2313 6 0.10932350729400753 2313 7 -0.04153215390971354 2313 8 0.2825970470619268 2313 9 0.40318021687861716 2313 10-11 -0.6994836376068321 2313 12-13 0.022236425338320487 2313 14-15 0.04971720765485799 2313 16-17 -0.47114129751037126 2313 18-19 -0.035251326927571824 2313 20-21 -2.395236390398601 2313 22-23 -0.42877014528662016 2313 24-25 0.055990028917250356 2313 26-27 0.02853850294127369 2313 28-29 -0.18347534392787423 2313 30-31 0.01280982532049535 2313 32-33 0.29711922346913155 2313 34-35 0.2120626763794533 2313 36-37 -0.6364885282743415 2313 38-39 -0.11328841282038837 2313 40-41 0.09256211834939165 2313 42-43 0.05110586089187308 2313 44-45 0.00840772235095244 2313 46-47 0.09623601344103605 2313 48-49 -0.22390148903771134 2313 50-51 -0.013338215131053488 2313 52-53 0.024058922625044943 2313 54-55 0.015898076022729413 2313 56-57 0.1038887110887039 2313 58-59 0.17900354218333803 2313 60-61 -0.04055764571266707 2313 62-63 -0.1382121623627981 2313 64-65 -0.15742159883589224 2313 66-67 0.22357727370609126 2313 68-69 0.12652506415142284 2313 70-71 0.22875006660885333 2313 72-73 0.07785361699688664 2313 74-75 0.17712179403552852 2313 76-77 0.32373341770147945 2313 78-79 0.17274960939872486 2313 80-81 0.3384428109747404 2313 82-83 0.5153078061812231 2313 84-85 0.40554064347590213 2313 86-87 0.5114446963369765 2313 88-89 0.7609415155241663 2313 90-91 1.023061013727851 2313 92-93 1.2554845490959146 2313 94-95 1.4354588675502136 2313 96-97 2.695230765952255 2313 98-99 3.189881224591618 2313 100-101 2.651961064137767 2314 1 -0.16316959731180702 2314 2 -0.0739491950879092 2314 3 -1.1048763952951504 2314 4 -0.06676582797693698 2314 5 -0.39824873441144604 2314 6 -0.399835542217275 2314 7 -0.18541385813168887 2314 8 -1.6470022496888532 2314 9 -1.383040287024798 2314 10-11 -0.6202555767136815 2314 12-13 -0.3733582088039924 2314 14-15 -0.5492329246636558 2314 16-17 -0.5028846960522557 2314 18-19 -1.6139089623163656 2314 20-21 -1.3244717997684603 2314 22-23 -1.7680456865248928 2314 24-25 -1.4648915402764615 2314 26-27 -1.6457389351225444 2314 28-29 -2.7901617688112843 2314 30-31 -1.3792834893109713 2314 32-33 -1.300209100040206 2314 34-35 -1.6031319985468997 2314 36-37 -2.9434709589850954 2314 38-39 -3.318774877385575 2314 40-41 -4.049918552860472 2314 42-43 -2.814229583291393 2314 44-45 -3.2961154947019224 2314 46-47 -2.9684459674510855 2314 48-49 -1.4112393747523626 2314 50-51 -0.9499658705720044 2314 52-53 -2.075857212884543 2314 54-55 -1.4717123003938042 2314 56-57 -1.4113726053357851 2314 58-59 -3.3134470462000074 2314 60-61 -1.988377077105909 2314 62-63 -1.6587901016845876 2314 64-65 -1.0988794080161632 2314 66-67 -2.2248713710051646 2314 68-69 -2.08053052983054 2314 70-71 -1.784656586150824 2314 72-73 -1.2345395430409951 2314 74-75 -1.5578532349220637 2314 76-77 -0.44130602065444435 2314 78-79 -0.24659562295551396 2314 80-81 -0.217857772953586 2314 82-83 -0.03725401058267508 2314 84-85 -0.11439705981281634 2314 86-87 0.1585160528014775 2314 88-89 0.36142793875417567 2314 90-91 0.8331710479809367 2314 92-93 0.9991194822844811 2314 94-95 1.5125233072610165 2314 96-97 3.133414690975746 2314 98-99 3.27802914207925 2314 100-101 3.0994610664341646 2315 1 -2.2711900022233387 2315 2 -1.7674541636297327 2315 3 -1.4090927424967568 2315 4 -1.6582727481618775 2315 5 -1.8917013540472567 2315 6 -2.959943164424484 2315 7 -2.0771984005266333 2315 8 -1.1410830003877948 2315 9 -1.1364392685651126 2315 10-11 -3.443458168933283 2315 12-13 -1.7339569229072538 2315 14-15 -1.0882772278553503 2315 16-17 -1.9022440637578342 2315 18-19 -1.3335147158964489 2315 20-21 -0.7747792658777044 2315 22-23 -0.949032681058668 2315 24-25 -2.8178499929365586 2315 26-27 -4.187673031868123 2315 28-29 -5.698661545714533 2315 30-31 -8.61036316875484 2315 32-33 -6.988710646090915 2315 34-35 -6.180347193012203 2315 36-37 -4.027880221392916 2315 38-39 -6.318814920392043 2315 40-41 -4.084914187196105 2315 42-43 -4.1412740717691925 2315 44-45 -3.307570305959839 2315 46-47 -2.235585281116748 2315 48-49 -2.7934865659517207 2315 50-51 -2.8989817753193172 2315 52-53 -2.04117536503513 2315 54-55 -3.6105924691157796 2315 56-57 -3.505359680513667 2315 58-59 -2.288601407106647 2315 60-61 -2.355331188378589 2315 62-63 -3.04012062893797 2315 64-65 -2.3995100225828274 2315 66-67 -2.8063496806712678 2315 68-69 -2.053878211675322 2315 70-71 -2.6222831715818096 2315 72-73 -1.884206488473957 2315 74-75 -2.3499743618076074 2315 76-77 -1.6027518885091752 2315 78-79 -1.365381178564089 2315 80-81 -1.2200570688105756 2315 82-83 -1.0388706597671522 2315 84-85 -1.0536868391911938 2315 86-87 -1.456327150226052 2315 88-89 -1.8581298190272157 2315 90-91 -1.6508843012205894 2315 92-93 -0.8452704646048161 2315 94-95 -0.20764193222825256 2315 96-97 1.226026072621318 2315 98-99 1.1274095358512906 2315 100-101 0.7822946435136036 2316 1 -3.604231458937953 2316 2 -2.1921182682579605 2316 3 -2.3935724800999587 2316 4 -3.0462993123767816 2316 5 -2.391513685848352 2316 6 -5.909729686033032 2316 7 -3.7768608955069496 2316 8 -4.727455032634342 2316 9 -6.745372981961442 2316 10-11 -5.530634885571608 2316 12-13 -3.5556037006526466 2316 14-15 -5.051650573500162 2316 16-17 -4.602675700615308 2316 18-19 -2.646138905350327 2316 20-21 -3.3479605591265233 2316 22-23 -4.027182762024893 2316 24-25 -2.912781816438759 2316 26-27 -3.795513440767074 2316 28-29 -3.8451003811407816 2316 30-31 -3.5021914794284257 2316 32-33 -3.274168398322743 2316 34-35 -5.381381052188669 2316 36-37 -5.676256356473825 2316 38-39 -5.53106644378888 2316 40-41 -4.804249975754246 2316 42-43 -4.232299754306428 2316 44-45 -3.6857628983787514 2316 46-47 -3.776370874898184 2316 48-49 -4.213531399763479 2316 50-51 -5.090470468891855 2316 52-53 -2.9412835464009532 2316 54-55 -3.3514418533370645 2316 56-57 -2.6886794574481065 2316 58-59 -3.054455089925245 2316 60-61 -3.1686684611623654 2316 62-63 -2.3979121436185196 2316 64-65 -2.7403483160508593 2316 66-67 -3.478691519272523 2316 68-69 -2.802572582522572 2316 70-71 -2.257981096308349 2316 72-73 -2.2362614692084257 2316 74-75 -3.243645765863473 2316 76-77 -3.260429043753298 2316 78-79 -3.387805993119624 2316 80-81 -3.538981541153099 2316 82-83 -2.6235133439825127 2316 84-85 -2.5677462763243035 2316 86-87 -2.237849452874844 2316 88-89 -2.2042756240986705 2316 90-91 -1.7161951139978804 2316 92-93 -1.361243894062973 2316 94-95 -1.4073367132100678 2316 96-97 0.22523953165977773 2316 98-99 0.686751113559323 2316 100-101 0.32092935690137203 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26917.0 3 8180.0 4 7648.0 5 8430.0 6 9956.0 7 12368.0 8 15272.0 9 18908.0 10 22857.0 11 27993.0 12 33611.0 13 38414.0 14 42016.0 15 47373.0 16 53514.0 17 56371.0 18 49435.0 19 46018.0 20 48223.0 21 52836.0 22 58108.0 23 64836.0 24 72202.0 25 81149.0 26 90762.0 27 102628.0 28 115159.0 29 129060.0 30 145851.0 31 164801.0 32 187765.0 33 214809.0 34 243898.0 35 265736.0 36 259877.0 37 190296.0 38 44660.0 39 430.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.9913410701717 19.38919352462639 13.87641792021999 21.74304748498191 2 15.663857832992786 21.02592371695703 40.41820218495879 22.892016265091396 3 19.110825337136717 26.964193168510576 29.944478147294152 23.98050334705856 4 12.305177572094818 16.897386017480688 37.94343089175864 32.85400551866585 5 12.70317490149443 39.002606951477695 33.84970312447817 14.444515022549705 6 30.015886705394607 37.10560729976432 18.206321546655246 14.672184448185831 7 27.395196884763152 34.58870366447023 21.03714832183053 16.97895112893608 8 26.772270795478992 34.04655850626129 20.264407625475577 18.916763072784136 9 28.025105388993698 16.112751568841208 20.415517372933113 35.44662566923198 10-11 24.600102854903035 27.349007381419284 27.257498936167252 20.79339082751043 12-13 26.241488799278677 25.146262406701585 27.772592144947833 20.839656649071898 14-15 23.181085883055346 25.850454398042306 26.945043481848224 24.02341623705412 16-17 21.167170278554647 29.295215667964207 27.682795398782194 21.854818654698946 18-19 21.136062607856143 27.150970191546126 31.192074527111345 20.520892673486387 20-21 22.224902968978903 26.229250692517148 31.187856905123184 20.35798943338077 22-23 21.692679544887124 26.417476879686564 31.077908231703123 20.811935343723196 24-25 21.110061162294976 26.712554909983595 31.519907690437222 20.657476237284207 26-27 20.903712901665973 26.825698560382357 31.299857229491835 20.970731308459836 28-29 20.897535937454425 27.12625227645663 31.29379424076546 20.682417545323485 30-31 20.94726846537724 27.05275775250483 31.12395241126681 20.876021370851124 32-33 20.59105278440333 27.1031895069606 30.987281561607148 21.31847614702892 34-35 21.31144533244568 27.08916137041195 30.888442258141612 20.710951039000758 36-37 21.40022927834322 26.647473327433485 30.834088012549216 21.11820938167408 38-39 20.970357999097363 27.08234872046059 30.87525361033821 21.072039670103837 40-41 21.18326299851075 26.86071149880984 31.10379808041953 20.852227422259876 42-43 20.85393955595392 26.992267916275182 30.663009307853688 21.49078321991721 44-45 20.942035561604108 27.214262383216532 30.65515046339903 21.18855159178033 46-47 21.36796710427471 27.421618777839985 29.763392806673387 21.44702131121192 48-49 20.99015492187537 27.65972544167 29.537020604083388 21.813099032371245 50-51 21.23636320724024 27.77255023697669 29.50444606852418 21.48664048725889 52-53 21.29456966448868 27.78504101575531 29.315162542522728 21.605226777233277 54-55 20.907043343272623 27.853884272593305 29.4659668184061 21.77310556572798 56-57 21.01237047447246 28.155185478457863 28.996282040525234 21.83616200654445 58-59 20.93534772271848 27.54677320323995 29.28618562329778 22.231693450743794 60-61 20.7270300861446 27.810733188303676 29.416538786858997 22.045697938692722 62-63 20.905599835928644 27.55478150219971 29.603232677457434 21.9363859844142 64-65 20.750525047148596 27.667126495823375 29.42008456444919 22.162263892578842 66-67 20.64094284395088 27.924673115447966 29.015322884842266 22.419061155758886 68-69 21.12388323955573 27.608960258618815 29.114910990857233 22.15224551096822 70-71 20.923557301064996 27.64085291462074 28.923170065299352 22.512419719014915 72-73 20.865820841667208 27.780527060424387 28.731858967699353 22.62179313020905 74-75 20.933031193525988 28.151756912824734 28.488886332270862 22.426325561378416 76-77 21.020212366803587 27.88785036924627 28.64109538114742 22.450841882802727 78-79 21.10268886102293 28.005757993493656 28.436001324935084 22.45555182054833 80-81 21.235831262124847 28.160524285097065 28.13847910904052 22.465165343737564 82-83 21.28941446886318 28.105392949447825 28.076612108178594 22.5285804735104 84-85 21.227039830565968 28.036323960645 28.053104799287077 22.683531409501953 86-87 21.41305279329934 28.02334534585021 27.877314039897417 22.686287820953037 88-89 21.537055083291122 27.88207250282706 27.71707809303889 22.863794320842928 90-91 21.42204364287663 27.99769274320216 27.834906599524622 22.745357014396596 92-93 21.78452867460489 27.746685246254653 27.71341766692008 22.755368412220378 94-95 21.50727479344464 27.590115891266677 27.84145345748124 23.061155857807446 96-97 21.929141123345893 27.264793379436153 27.590187140789364 23.215878356428593 98-99 21.880395169077055 27.35478631850717 27.25754208559854 23.507276426817235 100-101 22.01209051816038 27.399439755696747 27.496252464189304 23.092217261953575 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5841.0 1 3627.5 2 1579.5 3 1740.5 4 1507.5 5 1427.5 6 2288.0 7 4294.0 8 6522.0 9 7190.0 10 6156.5 11 5153.0 12 4929.5 13 5036.5 14 5259.0 15 5524.0 16 5838.5 17 6302.0 18 6859.5 19 7588.0 20 8500.0 21 9786.0 22 11485.0 23 13579.5 24 16005.0 25 19089.0 26 23784.0 27 29064.0 28 32757.5 29 36532.5 30 41857.5 31 48675.0 32 57728.0 33 63884.0 34 67625.5 35 73735.0 36 82756.0 37 90607.5 38 93718.0 39 95399.0 40 96597.0 41 97655.0 42 98362.0 43 100128.0 44 102146.0 45 103656.0 46 104197.0 47 105301.5 48 108883.0 49 107205.5 50 102266.5 51 98879.0 52 95060.5 53 92268.0 54 89212.5 55 82891.5 56 75962.0 57 69449.0 58 63351.5 59 56106.5 60 46590.5 61 38243.5 62 31057.5 63 22903.0 64 15760.0 65 11602.0 66 8733.5 67 6676.0 68 5025.0 69 3636.5 70 2667.5 71 1923.0 72 1431.0 73 1126.5 74 896.5 75 675.5 76 475.5 77 323.0 78 223.5 79 161.0 80 107.5 81 73.5 82 52.5 83 28.0 84 18.5 85 15.0 86 10.0 87 7.0 88 9.0 89 7.0 90 3.0 91 1.5 92 1.0 93 2.5 94 3.0 95 3.0 96 2.5 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.12153544685775122 2 0.11381891054932257 3 0.12732284908907268 4 0.14282131608142515 5 0.13814561823352134 6 0.1386687732374826 7 0.14628721798266853 8 0.14537169672573633 9 0.1530228386586698 10-11 0.14878855284535833 12-13 0.1479057287761737 14-15 0.13606934681155008 16-17 0.13937176277405558 18-19 0.15761679353720465 20-21 0.18751837173236569 22-23 0.20563261374452446 24-25 0.22546345811343113 26-27 0.22778495844350927 28-29 0.22966504673899504 30-31 0.22935442345539303 32-33 0.2304824764326845 34-35 0.20048280667428076 36-37 0.18786169220371524 38-39 0.2025427295023782 40-41 0.1904611186296478 42-43 0.192619133020988 44-45 0.19716404211790145 46-47 0.21787771055599278 48-49 0.2370055653883265 50-51 0.2534195536376112 52-53 0.274018781918586 54-55 0.2675283901506915 56-57 0.23643336460274386 58-59 0.2295506065818785 60-61 0.20754539922775783 62-63 0.19737657383826074 64-65 0.18912053393199704 66-67 0.18459197342895736 68-69 0.191066016602978 70-71 0.1949733305388137 72-73 0.23103832862439336 74-75 0.24397006637856083 76-77 0.2626074633946809 78-79 0.25018253205060087 80-81 0.2554631278718349 82-83 0.2526348211316693 84-85 0.2376431605494043 86-87 0.22778495844350927 88-89 0.23056421940205346 90-91 0.23295111410762673 92-93 0.2402752841630844 94-95 0.24490193622936685 96-97 8.65914718540973 98-99 0.3054898251256308 100-101 0.31706462958827375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3058367.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.72799645481924 #Duplication Level Percentage of deduplicated Percentage of total 1 73.64713280483463 34.413829606119144 2 12.529931313238702 11.709971719682933 3 5.173449592449355 7.2523480264547855 4 2.672941704793193 4.996048420220593 5 1.562170299286051 3.649854410343125 6 0.9656286464948812 2.707313518205083 7 0.6638463240210102 2.1714146072778986 8 0.47970610315984086 1.793256407024656 9 0.34375279346849613 1.4456591383077089 >10 1.7555476213168955 14.38992514032735 >50 0.12155179057212218 3.922816749142804 >100 0.07701631856339125 6.64178824122786 >500 0.004883125201065208 1.5446352856915722 >1k 0.0022261306062089773 1.8983793154320205 >5k 0.0 0.0 >10k+ 2.1543199414925587E-4 1.462759414542239 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19766 0.6462926130186469 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13729 0.44889969058651236 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10097 0.33014350468730536 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3992 0.13052717348833545 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3093 0.1011324017032619 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.634859387378951E-5 0.0 12-13 0.0 0.0 0.0 1.1444015711652657E-4 1.634859387378951E-5 14-15 0.0 0.0 0.0 1.3078875099031609E-4 3.269718774757902E-5 16-17 0.0 0.0 0.0 1.9618312648547411E-4 3.269718774757902E-5 18-19 0.0 0.0 0.0 2.9427468972821114E-4 3.269718774757902E-5 20-21 0.0 0.0 0.0 6.539437549515803E-4 3.269718774757902E-5 22-23 0.0 0.0 0.0 0.0015367678241362138 3.269718774757902E-5 24-25 0.0 0.0 0.0 0.0036620850277288503 3.269718774757902E-5 26-27 0.0 0.0 0.0 0.007013546771855699 3.269718774757902E-5 28-29 0.0 0.0 0.0 0.01798345326116846 3.269718774757902E-5 30-31 0.0 0.0 0.0 0.05084412694748538 3.269718774757902E-5 32-33 0.0 0.0 0.0 0.11314861820049718 3.269718774757902E-5 34-35 0.0 0.0 0.0 0.209163910021263 3.269718774757902E-5 36-37 0.0 0.0 0.0 0.35888433271742726 3.269718774757902E-5 38-39 0.0 0.0 0.0 0.5854267980265285 3.269718774757902E-5 40-41 0.0 0.0 0.0 0.839843615890441 3.269718774757902E-5 42-43 0.0 3.269718774757902E-5 0.0 1.0871978412008763 3.269718774757902E-5 44-45 0.0 3.269718774757902E-5 0.0 1.3690966453666287 3.269718774757902E-5 46-47 0.0 3.269718774757902E-5 0.0 1.6977360794175453 3.269718774757902E-5 48-49 0.0 3.269718774757902E-5 0.0 2.0558356796290305 3.269718774757902E-5 50-51 0.0 3.269718774757902E-5 0.0 2.448365418538717 3.269718774757902E-5 52-53 0.0 3.269718774757902E-5 0.0 2.8106339101880184 3.269718774757902E-5 54-55 0.0 3.269718774757902E-5 0.0 3.185736048028245 3.269718774757902E-5 56-57 1.634859387378951E-5 3.269718774757902E-5 0.0 3.6105869570264133 3.269718774757902E-5 58-59 3.269718774757902E-5 3.269718774757902E-5 0.0 4.068658208776121 3.269718774757902E-5 60-61 3.269718774757902E-5 3.269718774757902E-5 0.0 4.574974161047383 3.269718774757902E-5 62-63 3.269718774757902E-5 3.269718774757902E-5 0.0 5.0532359262312205 3.269718774757902E-5 64-65 3.269718774757902E-5 3.269718774757902E-5 0.0 5.516456985051173 3.269718774757902E-5 66-67 3.269718774757902E-5 3.269718774757902E-5 0.0 6.031830058328513 3.269718774757902E-5 68-69 3.269718774757902E-5 3.269718774757902E-5 0.0 6.609752851766972 3.269718774757902E-5 70-71 3.269718774757902E-5 3.269718774757902E-5 0.0 7.214291156031961 3.269718774757902E-5 72-73 3.269718774757902E-5 3.269718774757902E-5 0.0 7.815265466832463 3.269718774757902E-5 74-75 3.269718774757902E-5 3.269718774757902E-5 0.0 8.386632474127532 3.269718774757902E-5 76-77 3.269718774757902E-5 3.269718774757902E-5 0.0 8.972778610284507 3.269718774757902E-5 78-79 3.269718774757902E-5 3.269718774757902E-5 0.0 9.624368167718263 3.269718774757902E-5 80-81 3.269718774757902E-5 3.269718774757902E-5 0.0 10.31480198419614 4.904578162136853E-5 82-83 3.269718774757902E-5 3.269718774757902E-5 0.0 11.004696297076183 6.539437549515804E-5 84-85 3.269718774757902E-5 3.269718774757902E-5 0.0 11.653948005585988 6.539437549515804E-5 86-87 3.269718774757902E-5 3.269718774757902E-5 0.0 12.230383076981932 6.539437549515804E-5 88-89 3.269718774757902E-5 3.269718774757902E-5 0.0 12.802354982250332 6.539437549515804E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 19700 0.0 38.372414 1 GGTATCA 11680 0.0 37.777172 1 TATCAAC 24785 0.0 30.462301 2 ATCAACG 25315 0.0 29.899355 3 TCAACGC 25400 0.0 29.781752 4 CAACGCA 25745 0.0 29.383621 5 GTACATG 20265 0.0 28.690712 1 AACGCAG 26550 0.0 28.548199 6 TACATGG 20030 0.0 28.370213 2 ACATGGG 20550 0.0 26.737125 3 CATGGGA 14140 0.0 25.28495 4 ACGCAGA 29525 0.0 25.161695 7 CGCAGAG 29820 0.0 24.80193 8 ATGGGAT 5505 0.0 23.244688 5 GCAGAGT 30395 0.0 22.784855 9 AGTACTT 16425 0.0 22.025692 12-13 GAGTACT 15780 0.0 21.852724 12-13 GTATCGC 130 3.1392492E-4 21.71479 6 CATGGGG 6175 0.0 20.721914 4 ATGGGAG 5290 0.0 20.632149 5 >>END_MODULE