FastQCFastQC Report
Fri 27 May 2016
SRR939752_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939752_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3058367
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219770.7185860951285441No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168790.5518958319913863No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125620.4107420724850876No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48550.15874484651449614No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38050.12441279937953817No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36350.11885427746244973No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130200.039.8768161
GTATCAA207450.035.2307171
TATCAAC262950.027.6087762
ATCAACG263200.027.5054953
TCAACGC264800.027.3213844
CAACGCA269250.026.9050735
GTACATG206200.026.3070241
AACGCAG276400.026.2262466
TACATGG206500.025.8040942
ACATGGG208400.024.5853773
TAAGGTG15250.024.5758235
GCGTATC1201.7218909E-423.7292581
ACGCAGA310900.023.2701917
CGCAGAG312800.023.1136768
GTAAGGT15250.022.3982184
CATGGGA146650.021.9653684
GAGTACT173250.021.81038712-13
GCAGAGT329650.021.4141439
ATGGGAT52100.021.3073275
CATGGGG60350.021.3031024
Produced by FastQC (version 0.11.5)