##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939752_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3058367 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.395305403177577 31.0 31.0 34.0 30.0 34.0 2 31.603882071706895 31.0 31.0 34.0 30.0 34.0 3 31.634822439556796 31.0 31.0 34.0 30.0 34.0 4 35.45910284802314 37.0 35.0 37.0 33.0 37.0 5 35.20009861471825 37.0 35.0 37.0 32.0 37.0 6 35.23897360911885 37.0 35.0 37.0 32.0 37.0 7 35.14541126032291 37.0 35.0 37.0 32.0 37.0 8 35.105414752382565 37.0 35.0 37.0 32.0 37.0 9 36.662892321294336 39.0 35.0 39.0 32.0 39.0 10-11 36.54025448875168 38.0 35.0 39.0 32.0 39.0 12-13 36.454088080338295 38.0 35.0 39.0 32.0 39.0 14-15 37.422644012311146 39.0 36.0 41.0 32.0 41.0 16-17 37.36374019206982 39.0 36.0 41.0 32.0 41.0 18-19 37.41552469013693 39.0 36.0 41.0 32.0 41.0 20-21 37.36010508222198 39.0 36.0 41.0 31.5 41.0 22-23 37.22860173419345 39.0 36.0 40.0 31.0 41.0 24-25 37.06494985722773 39.0 36.0 40.0 31.0 41.0 26-27 36.7516414805679 39.0 35.5 40.0 30.5 41.0 28-29 36.51534740598496 38.0 35.0 40.0 30.0 41.0 30-31 36.18981959980604 38.0 35.0 40.0 30.0 41.0 32-33 36.07550385548889 38.0 35.0 40.0 30.0 41.0 34-35 35.88069679014978 38.0 35.0 40.0 29.5 41.0 36-37 35.8316264856376 38.0 35.0 40.0 29.0 41.0 38-39 35.6852365984854 38.0 35.0 40.0 29.0 41.0 40-41 35.55625829078067 38.0 34.0 40.0 27.5 41.0 42-43 35.2688021090994 38.0 34.0 40.0 27.0 41.0 44-45 34.92807109153349 38.0 34.0 40.0 25.5 41.0 46-47 34.523336963811076 38.0 33.0 40.0 24.0 41.0 48-49 34.124433562093756 38.0 33.0 40.0 23.5 41.0 50-51 33.85879523288081 38.0 33.0 40.0 22.5 41.0 52-53 33.58788088545292 37.0 33.0 40.0 21.5 41.0 54-55 33.31514334283622 37.0 32.0 40.0 20.0 41.0 56-57 32.949698973341 37.0 31.5 40.0 18.5 41.0 58-59 32.5899686008906 36.0 31.0 39.0 18.0 41.0 60-61 32.27362412686247 36.0 31.0 39.0 15.0 40.5 62-63 31.98257256241648 35.5 31.0 39.0 13.0 40.0 64-65 31.544370410745344 35.0 30.5 39.0 9.5 40.0 66-67 30.678437708751108 34.5 29.0 38.0 7.5 40.0 68-69 28.875721422576166 34.0 25.5 37.0 4.0 39.5 70-71 28.236595542653973 33.0 24.5 36.0 2.0 39.0 72-73 28.76367404565901 33.5 26.0 36.0 2.0 39.0 74-75 28.834643128179188 34.0 26.5 36.0 2.0 39.0 76-77 28.339628141423184 32.5 26.5 35.0 2.0 37.0 78-79 28.850298051214914 34.0 28.0 35.0 2.0 37.0 80-81 28.664711265848737 34.0 28.0 35.0 2.0 37.0 82-83 28.232141368253057 33.5 27.0 35.0 2.0 36.5 84-85 27.884509445727083 33.0 26.5 35.0 2.0 36.0 86-87 27.514974658044636 33.0 26.0 35.0 2.0 36.0 88-89 27.061736868073712 33.0 25.5 35.0 2.0 35.0 90-91 26.579879393153277 32.0 25.0 35.0 2.0 35.0 92-93 26.117903770214628 32.0 24.0 34.5 2.0 35.0 94-95 25.36215454195 32.0 20.0 34.0 2.0 35.0 96-97 24.85699018463121 32.0 18.5 34.0 2.0 35.0 98-99 24.208312475252317 31.0 8.5 34.0 2.0 35.0 100-101 22.754344066621172 30.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2311273886541514 1101 2 0.32904303734721196 1101 3 0.2995560062537166 1101 4 0.16054085040529742 1101 5 0.23157350637569607 1101 6 0.1660166250123254 1101 7 0.1911552305424138 1101 8 0.07090066174502141 1101 9 0.19935726473811854 1101 10-11 0.36261796877047914 1101 12-13 0.3387886429873248 1101 14-15 0.3588640805290808 1101 16-17 0.4293032730751136 1101 18-19 0.2470545897554146 1101 20-21 0.40352978261385175 1101 22-23 0.29124614888898037 1101 24-25 0.19669738579016638 1101 26-27 0.25370581659759495 1101 28-29 0.2044554419676885 1101 30-31 0.07051172302059427 1101 32-33 0.1331892378644497 1101 34-35 0.005565368484525379 1101 36-37 0.1429358447278375 1101 38-39 0.12585017123648612 1101 40-41 0.06490027910184182 1101 42-43 0.06749040160719488 1101 44-45 -0.012708123199089982 1101 46-47 0.014302746146185541 1101 48-49 0.12237704650924996 1101 50-51 0.05935030377609252 1101 52-53 -0.13684611502604582 1101 54-55 -0.030182435344094927 1101 56-57 -0.22927551309708605 1101 58-59 -0.2033669593771279 1101 60-61 -0.2939386768357508 1101 62-63 -0.17094612237034568 1101 64-65 -0.386820339017234 1101 66-67 -0.5098617447741027 1101 68-69 -8.825067816602694 1101 70-71 -6.373169261278914 1101 72-73 -1.7824942654168545 1101 74-75 -0.8408135732724666 1101 76-77 -0.7221589038437379 1101 78-79 -0.8692433511532727 1101 80-81 -0.9410106250681309 1101 82-83 -0.8709351706532296 1101 84-85 -0.6826528678675352 1101 86-87 -0.7187314767122572 1101 88-89 -0.6312946954186067 1101 90-91 -1.0489147979124027 1101 92-93 -1.108684614965739 1101 94-95 -0.6444078381856535 1101 96-97 -1.1545963222298568 1101 98-99 -0.8349608646947324 1101 100-101 -0.610033605145528 1102 1 0.1487926514094582 1102 2 0.009315634023533192 1102 3 0.1694798872057639 1102 4 0.13618543695106666 1102 5 0.18135662305891742 1102 6 0.20006170875727491 1102 7 0.20075138447825935 1102 8 0.11685660411469456 1102 9 0.29131450840768736 1102 10-11 0.296889497354762 1102 12-13 0.16406872563619856 1102 14-15 0.2938343581863663 1102 16-17 0.33067943270123834 1102 18-19 0.13874673438160556 1102 20-21 0.12082826769554345 1102 22-23 0.21085018525074872 1102 24-25 0.12136716344978282 1102 26-27 0.14903961867874216 1102 28-29 0.07818177719932606 1102 30-31 0.10845668363178618 1102 32-33 0.0045589492930346864 1102 34-35 -0.040671297855645605 1102 36-37 -0.05807505307580385 1102 38-39 0.21504238582503632 1102 40-41 0.12166493971443515 1102 42-43 0.2553958334848403 1102 44-45 0.19571680252420265 1102 46-47 0.05812279595939174 1102 48-49 0.06523913670472581 1102 50-51 0.02301418594081639 1102 52-53 0.015817585236547416 1102 54-55 -0.15031101450184536 1102 56-57 -0.2869397730585632 1102 58-59 -0.22671590597719415 1102 60-61 -0.2039717457113568 1102 62-63 -0.14959314360625342 1102 64-65 -0.25832466968733314 1102 66-67 0.12835653545779735 1102 68-69 -4.35539515882591 1102 70-71 0.10944217710057202 1102 72-73 0.3514757836967206 1102 74-75 0.15195348471700143 1102 76-77 0.03533686343346787 1102 78-79 -0.30657136035098986 1102 80-81 -0.180593929250481 1102 82-83 -0.021410856400958522 1102 84-85 -0.1073204300524111 1102 86-87 -0.3179173480468158 1102 88-89 -0.3578225567625388 1102 90-91 -0.37016714748166635 1102 92-93 -0.4110626938926103 1102 94-95 -0.17398372635781456 1102 96-97 -0.6563900988447031 1102 98-99 -0.4333415858667422 1102 100-101 -0.3994702798811893 1103 1 0.18417710032192147 1103 2 0.19653158383366076 1103 3 0.19833543779475704 1103 4 0.1470103190990244 1103 5 0.22111263066626208 1103 6 0.20160799100052174 1103 7 0.18014675152342363 1103 8 0.13308469292592662 1103 9 0.32461254703018483 1103 10-11 0.30929929897356345 1103 12-13 0.3017630235858775 1103 14-15 0.38832781676776307 1103 16-17 0.3358619766057629 1103 18-19 0.2997997291349819 1103 20-21 0.31335615646565174 1103 22-23 0.21550081576837954 1103 24-25 0.2257408749520593 1103 26-27 0.189638223772846 1103 28-29 0.11749079400652818 1103 30-31 0.14046904301238783 1103 32-33 0.18712075611029633 1103 34-35 0.06680149320815332 1103 36-37 0.2676130379902091 1103 38-39 0.4066447373705486 1103 40-41 0.3323175972701904 1103 42-43 0.11703824424389353 1103 44-45 0.17139172180809226 1103 46-47 0.1073135386609465 1103 48-49 0.04415068861259641 1103 50-51 -0.10740373629410982 1103 52-53 -0.10644131024861991 1103 54-55 -0.15650129888202713 1103 56-57 0.10556653098469582 1103 58-59 -0.0647244571548029 1103 60-61 -0.23764437282189022 1103 62-63 -0.15395161549823655 1103 64-65 -0.22244133598575644 1103 66-67 8.655248450253339E-4 1103 68-69 -3.94864752393978 1103 70-71 0.4119052720331453 1103 72-73 0.39515970171554926 1103 74-75 0.19122777334161967 1103 76-77 0.15099428832456496 1103 78-79 -0.08660169227423253 1103 80-81 -0.02562986697089542 1103 82-83 -0.04056427055521539 1103 84-85 -0.05794176992574407 1103 86-87 -0.21325544361673465 1103 88-89 -0.08921870335059268 1103 90-91 -0.2778967458074604 1103 92-93 -0.3316204183072031 1103 94-95 -0.5277096411168394 1103 96-97 -0.7628630808285415 1103 98-99 -0.5709744721526242 1103 100-101 -0.744934626274695 1104 1 0.1751570801348592 1104 2 0.17393028263761323 1104 3 0.18568153034525636 1104 4 0.17652825442373654 1104 5 0.2527010090100461 1104 6 0.19607669268293648 1104 7 0.16061908990136686 1104 8 0.18720340991875872 1104 9 0.27552860654583355 1104 10-11 0.2280136995641584 1104 12-13 0.1264237507845678 1104 14-15 0.20768590274477816 1104 16-17 0.2532723188839441 1104 18-19 0.1086708035803099 1104 20-21 0.2719474040941563 1104 22-23 0.23090870730045765 1104 24-25 0.03605582660950546 1104 26-27 0.1054318728949113 1104 28-29 0.17244928847723173 1104 30-31 0.2796500898557639 1104 32-33 0.1999316560439226 1104 34-35 0.12066681882310348 1104 36-37 0.28355271658495695 1104 38-39 0.2809977171764686 1104 40-41 0.2943343269336651 1104 42-43 0.27489821040436624 1104 44-45 0.31391778326252506 1104 46-47 0.2577038056669778 1104 48-49 0.2730240744711736 1104 50-51 0.319535353106275 1104 52-53 0.28015179083088526 1104 54-55 0.36883546268759204 1104 56-57 0.2986193840409186 1104 58-59 0.2640860669041487 1104 60-61 0.25611178712142646 1104 62-63 0.3546740063016287 1104 64-65 0.36725256917393523 1104 66-67 0.4764486841473037 1104 68-69 -3.8029015234137695 1104 70-71 0.8800658785307007 1104 72-73 1.084569054622449 1104 74-75 0.8194161600021737 1104 76-77 0.7919823418823846 1104 78-79 0.4940724087183206 1104 80-81 0.5292503202751782 1104 82-83 0.4682980550829505 1104 84-85 0.5673417967361125 1104 86-87 0.5318225152757527 1104 88-89 0.46648133353481214 1104 90-91 0.4274217188740934 1104 92-93 0.3209487877277475 1104 94-95 0.6504226073644048 1104 96-97 0.27895848562918246 1104 98-99 0.3789973102440207 1104 100-101 0.5041642735898044 1105 1 0.21093514513085765 1105 2 0.2653810006603301 1105 3 0.20538226188732978 1105 4 0.1509585941132272 1105 5 0.1984376367863021 1105 6 0.208380940058575 1105 7 0.16959056580070353 1105 8 0.2604703049755628 1105 9 0.2719260804450485 1105 10-11 0.24784467019906486 1105 12-13 0.1909516606901036 1105 14-15 0.2368463354864332 1105 16-17 0.21535571849637591 1105 18-19 0.2563122321139417 1105 20-21 0.24265246298952547 1105 22-23 0.3333616507197661 1105 24-25 0.18114739402091118 1105 26-27 0.3555946058771582 1105 28-29 0.5356811112162845 1105 30-31 0.3778814102967516 1105 32-33 0.3649459570447604 1105 34-35 0.3892702161712407 1105 36-37 0.03225873242932664 1105 38-39 0.3204463530603263 1105 40-41 0.38437244360997624 1105 42-43 0.25675433937385606 1105 44-45 0.15624277929104835 1105 46-47 0.12294608569336418 1105 48-49 0.1014777374469773 1105 50-51 0.17853817388157722 1105 52-53 0.15713498540240067 1105 54-55 0.08217252557098931 1105 56-57 0.01979830556847162 1105 58-59 -0.023277188636761537 1105 60-61 0.10592429237236445 1105 62-63 0.1699299848873892 1105 64-65 0.07602652017472877 1105 66-67 0.6360046969188531 1105 68-69 1.8506986993894223 1105 70-71 2.0481542648614877 1105 72-73 1.1133320235846433 1105 74-75 0.6067599139387347 1105 76-77 0.20144398270099728 1105 78-79 0.20490704681440164 1105 80-81 0.187051768432692 1105 82-83 0.13194798167785393 1105 84-85 0.15028501892189894 1105 86-87 0.16500434749681148 1105 88-89 0.2581525988906108 1105 90-91 0.1627298162880848 1105 92-93 0.15429376931665217 1105 94-95 0.5065209013535146 1105 96-97 0.02455536596280794 1105 98-99 0.15250404341010437 1105 100-101 0.1209140539859277 1106 1 0.37415469941593216 1106 2 0.3330407474133601 1106 3 0.3254980431567063 1106 4 0.2867830143244987 1106 5 0.3348647741249593 1106 6 0.3618633314683066 1106 7 0.37944071655014255 1106 8 0.36772707747499567 1106 9 0.471168884672835 1106 10-11 0.38475816917382843 1106 12-13 0.4257031278432635 1106 14-15 0.6255187398388173 1106 16-17 0.5662242453182103 1106 18-19 0.5713383025439853 1106 20-21 0.5123367521163047 1106 22-23 0.5985704089854096 1106 24-25 0.5624864341701397 1106 26-27 0.6316990628861276 1106 28-29 0.5844028144620736 1106 30-31 0.6413491536510776 1106 32-33 0.677447501885176 1106 34-35 0.7737326133253433 1106 36-37 0.7003256046717539 1106 38-39 0.822089307708346 1106 40-41 0.6767487519481392 1106 42-43 0.6844564555003032 1106 44-45 0.7274012860161889 1106 46-47 0.8668481350935053 1106 48-49 0.8220828378111449 1106 50-51 0.7049819021317063 1106 52-53 0.7182791484529218 1106 54-55 0.6021798954716786 1106 56-57 0.6350621815814321 1106 58-59 0.807624552388944 1106 60-61 0.8099892653650471 1106 62-63 0.8268354188507345 1106 64-65 0.8542382204641399 1106 66-67 1.3059025491022567 1106 68-69 2.743845933016029 1106 70-71 3.063545138899549 1106 72-73 1.9251149275521833 1106 74-75 1.487023691189851 1106 76-77 1.0795749357334223 1106 78-79 1.0488263552526256 1106 80-81 0.9524286916848048 1106 82-83 0.9091795650483441 1106 84-85 0.9884978352741847 1106 86-87 1.0279697438270397 1106 88-89 1.18615887992841 1106 90-91 1.0369234635588924 1106 92-93 1.048363589735228 1106 94-95 1.3339916664966474 1106 96-97 1.0356685566494868 1106 98-99 1.16931978650236 1106 100-101 1.2535868918110928 1107 1 0.1697299880613059 1107 2 0.16659982264179263 1107 3 0.21459994360133905 1107 4 0.1405721590542015 1107 5 0.11734692647286948 1107 6 0.239923453279566 1107 7 0.20242502865863088 1107 8 0.19403485920875596 1107 9 0.2569568472894659 1107 10-11 0.22512301148002933 1107 12-13 0.21320325018217545 1107 14-15 0.31760046521326757 1107 16-17 0.30913725830654926 1107 18-19 0.2761192329069715 1107 20-21 0.18056813351627454 1107 22-23 0.3344678702507764 1107 24-25 0.3575685906031012 1107 26-27 0.3462616810495547 1107 28-29 0.38342822010600486 1107 30-31 0.3694336794165025 1107 32-33 0.3878480437508287 1107 34-35 0.4019914028977283 1107 36-37 0.09670406425671985 1107 38-39 0.33391368306711655 1107 40-41 0.2882081211729428 1107 42-43 0.24852082449320534 1107 44-45 0.31402941911582616 1107 46-47 0.3871880968556738 1107 48-49 0.2500119718771501 1107 50-51 0.31266972215436084 1107 52-53 0.3663483318544891 1107 54-55 0.4349866927494972 1107 56-57 0.47196674743998557 1107 58-59 0.40741720388388103 1107 60-61 0.5622695563742184 1107 62-63 0.4588760234560425 1107 64-65 0.4563939279043474 1107 66-67 0.9502423104413751 1107 68-69 2.2457206439238533 1107 70-71 2.389845314344189 1107 72-73 1.3744008725341388 1107 74-75 0.778290841510124 1107 76-77 0.4795635103424978 1107 78-79 0.6055683146071686 1107 80-81 0.530860702523821 1107 82-83 0.6351899674731669 1107 84-85 0.6374548268687583 1107 86-87 0.5640128096785624 1107 88-89 0.7631521851174092 1107 90-91 0.729024444014879 1107 92-93 0.7904789529924479 1107 94-95 1.1947814695160979 1107 96-97 0.8262872043342604 1107 98-99 1.2190307687923685 1107 100-101 1.3679093640018571 1108 1 -0.1302685587624559 1108 2 -0.23613989896193388 1108 3 -0.10355684765635331 1108 4 -0.11755472906575903 1108 5 -0.13538221923711546 1108 6 -0.026261700749039107 1108 7 -0.036844475792122466 1108 8 -0.05264180746329572 1108 9 -0.16766367188196796 1108 10-11 -0.06689800144838642 1108 12-13 -0.09906656266486635 1108 14-15 -0.13404368260381005 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1.3974817104881296 2316 70-71 1.6767677945922834 2316 72-73 0.7681560882297624 2316 74-75 0.28139742207298113 2316 76-77 -0.04171672133889359 2316 78-79 -0.21841037671190122 2316 80-81 -0.2646039066105921 2316 82-83 -0.09470589666405971 2316 84-85 -0.16183823832685817 2316 86-87 -0.3386698577230085 2316 88-89 -0.2521813794225167 2316 90-91 -0.2657606344895669 2316 92-93 -0.47791880853549884 2316 94-95 -0.26619603119223 2316 96-97 -0.22886563199779175 2316 98-99 -0.4380948197393728 2316 100-101 -0.6251093451531453 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 19.0 4 78.0 5 189.0 6 433.0 7 1111.0 8 2368.0 9 4288.0 10 7181.0 11 10853.0 12 14950.0 13 19343.0 14 23724.0 15 29379.0 16 35964.0 17 42884.0 18 36792.0 19 27869.0 20 25897.0 21 26276.0 22 27963.0 23 31649.0 24 36869.0 25 44712.0 26 54595.0 27 67671.0 28 81932.0 29 98343.0 30 117101.0 31 138233.0 32 165073.0 33 200773.0 34 249229.0 35 316749.0 36 393511.0 37 438084.0 38 263165.0 39 23111.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.004732299662166 20.293422201582228 14.698091055070625 22.00375444368498 2 15.657467710332137 21.884596100743174 39.42781769890144 23.030118490023245 3 18.84543501035194 26.976252663188554 30.623594836978203 23.554717489481305 4 12.514881307573617 17.585626577843666 37.60621272724954 32.29327938733317 5 12.37032704054157 39.35662397612843 34.047123840925565 14.225925142404428 6 30.06866082455114 37.646789937244286 17.728088224859867 14.556461013344704 7 27.135265322964834 34.7321626214251 20.96958932659161 17.16298272901846 8 26.63686209012849 34.63704650226739 20.058220612503337 18.667870795100786 9 27.309443242096194 16.082013702083497 20.465071719646467 36.143471336173846 10-11 24.39591128206654 27.59971252632532 27.28506748863037 20.719308702977767 12-13 25.91708254764716 25.23552601764275 27.90207977002106 20.945311664689033 14-15 22.637211296093636 26.088857223479067 27.045413451034488 24.22851802939281 16-17 20.84594767713514 29.52292685255515 27.836612926483074 21.794512543826634 18-19 20.849459858807005 27.317682933408584 31.26485474110857 20.568002466675843 20-21 21.868811035431655 26.371033953740675 31.302440158424417 20.45771485240326 22-23 21.402205817679828 26.523223014111778 31.208779064121472 20.865792104086918 24-25 20.91454093935812 26.91487118117033 31.52300330405298 20.647584575418566 26-27 20.64316243054072 27.000498470338563 31.35597501468517 21.000364084435546 28-29 20.654719320525615 27.260718475629453 31.338683034458946 20.745879169385987 30-31 20.769054455126664 27.150299523959465 31.15426832677588 20.926377694137987 32-33 20.43583406058993 27.178835149104223 31.041818371466213 21.343512418839637 34-35 21.11940363172402 27.199983519957282 30.908843292287454 20.77176955603125 36-37 21.27497869209388 26.740603900754255 30.877889005120444 21.10652840203142 38-39 20.778010040422863 27.173731258482796 30.95599042890259 21.092268272191745 40-41 20.96233625552934 26.976254586678134 31.134422707405573 20.92698645038696 42-43 20.664735721996337 27.115458009645337 30.68635819994671 21.53344806841161 44-45 20.77516890880152 27.275919887331945 30.69196395695808 21.256947246908453 46-47 21.177593329898052 27.47437405203097 29.82495269666251 21.52307992140847 48-49 20.812611274022096 27.68257121537414 29.619295569984814 21.885521940618947 50-51 21.025195766527368 27.802152343618324 29.55442108187945 21.618230807974857 52-53 21.219216746953496 27.680108738972148 29.410583856817425 21.69009065725693 54-55 20.80930387166198 27.772844742549545 29.523496061333077 21.894355324455397 56-57 20.9196392942885 28.025931038091933 29.016350814760543 22.038078852859023 58-59 20.742918687849002 27.508698298895773 29.332254824389935 22.416128188865294 60-61 20.575224422153013 27.82213391688107 29.394576851589036 22.208064809376875 62-63 20.7511232958054 27.613956710256794 29.553841930785392 22.081078063152415 64-65 20.540997795762934 27.624835646839436 29.391282652775274 22.442883904622356 66-67 20.26908836811189 27.83798049798604 29.256508229857747 22.636422904044316 68-69 20.589397760271442 27.447721174344164 29.50560391719425 22.45727714819014 70-71 20.396179179135647 27.50017847017825 29.19793450513707 22.905707845549035 72-73 20.17309859387894 27.541015071326324 29.022993070520915 23.26289326427382 74-75 20.319512350281226 27.817237980591937 28.60045845536487 23.262791213761968 76-77 20.126037536519732 27.619424531611187 28.942875902823374 23.311662029045706 78-79 20.13859811728783 27.654764927476 28.7325250241605 23.474111931075672 80-81 20.171656558707472 27.729966425967756 28.44737103687127 23.6510059784535 82-83 20.155534033532938 27.684423643213403 28.357348335679905 23.802693987573758 84-85 19.967341910060117 27.481498217668722 28.405753552401062 24.145406319870098 86-87 19.9674724866545 27.543721516354985 28.325405269666426 24.163400727324092 88-89 19.95037864241315 27.336394777528678 28.312620438303465 24.400606141754707 90-91 19.7819078323535 27.302229313941552 28.42674457385379 24.48911827985116 92-93 19.82263021940294 27.0399404898146 28.398799986920835 24.73862930386162 94-95 19.322500545227904 26.65659706128296 28.86946156024674 25.151440833242393 96-97 19.57089486345317 26.35395696063773 28.79225351652075 25.282894659388354 98-99 19.46404388297481 26.49536312259036 28.5160520627948 25.524540931640026 100-101 19.211000390119544 26.43955910278391 28.781617096196022 25.56782341090052 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3244.0 1 2140.0 2 1277.0 3 1676.5 4 1534.5 5 1458.0 6 2465.5 7 4766.5 8 7125.0 9 7862.0 10 6730.5 11 5393.5 12 5226.0 13 5402.0 14 5533.0 15 5744.0 16 5986.0 17 6288.5 18 6819.0 19 7588.0 20 8445.0 21 9626.5 22 11180.5 23 13077.0 24 15259.5 25 18187.0 26 22757.5 27 28029.5 28 31923.5 29 35838.0 30 40924.5 31 47587.0 32 57590.0 33 64064.0 34 67422.5 35 73429.5 36 82651.0 37 90976.5 38 94163.5 39 95698.0 40 96801.0 41 97811.0 42 98046.5 43 99959.5 44 102807.0 45 104854.5 46 105233.0 47 105113.5 48 109371.0 49 108438.0 50 103674.0 51 100110.5 52 95098.5 53 92930.0 54 91189.0 55 85029.0 56 76930.0 57 70030.0 58 64161.5 59 56988.5 60 47109.5 61 38083.0 62 30641.0 63 21926.5 64 14443.0 65 10550.5 66 7905.5 67 6053.0 68 4435.5 69 3049.0 70 2234.0 71 1562.0 72 1148.5 73 998.5 74 780.5 75 542.0 76 333.5 77 197.0 78 127.5 79 75.0 80 50.0 81 34.5 82 18.5 83 12.5 84 7.0 85 2.5 86 1.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021482052350159417 2 0.026484722075539008 3 9.809156324273706E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.269718774757902E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.539437549515803E-5 26-27 3.433204713495797E-4 28-29 9.809156324273706E-5 30-31 4.577606284661063E-4 32-33 0.0022888031423305313 34-35 0.00400540549907843 36-37 0.10342120484559245 38-39 0.09541039384743558 40-41 0.10422228594540812 42-43 0.10505606423297138 44-45 0.09874550699768864 46-47 0.0922551152297942 48-49 0.09308889351735747 50-51 0.09200988632168736 52-53 0.07327439774232458 54-55 0.06133992421445824 56-57 0.03358001181676365 58-59 0.014435808390556137 60-61 0.009514881634545494 62-63 0.0022070601729615837 64-65 0.005427733166098118 66-67 0.14471775297078474 68-69 0.3957994576844441 70-71 0.380578916787946 72-73 0.1717092814564112 74-75 0.03029394444813196 76-77 0.030882493827588384 78-79 0.0056402648864573805 80-81 0.00166755657512653 82-83 3.2697187747579015E-5 84-85 4.2506344071852726E-4 86-87 0.0010790071956701076 88-89 2.1253172035926363E-4 90-91 8.828240691846335E-4 92-93 0.0021907115790877943 94-95 0.07538336635204343 96-97 0.016839051690003194 98-99 0.019552918273052253 100-101 0.010790071956701076 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3058367.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.958616742447624 #Duplication Level Percentage of deduplicated Percentage of total 1 68.02030463917224 27.179972837812155 2 14.644682669383844 11.703625242013475 3 6.222389470220908 7.4591422818839686 4 3.3005432548833307 5.275405718550145 5 1.9709982658207452 3.9379182151980023 6 1.313027416286443 3.1480055579829727 7 0.8702682375960042 2.4342300478456855 8 0.618280209412828 1.976449754189396 9 0.4610617656701045 1.6581051350107112 >10 2.291133481425019 16.27643307112498 >50 0.16593162666424843 4.607342673066464 >100 0.10998347166654814 8.124624919159043 >500 0.00787026771658785 2.130930781567165 >1k 0.003279278215170721 2.403418940314231 >5k 0.0 0.0 >10k+ 2.459458661378041E-4 1.6843948242817226 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21977 0.7185860951285441 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16879 0.5518958319913863 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12562 0.4107420724850876 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4855 0.15874484651449614 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3805 0.12441279937953817 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3635 0.11885427746244973 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.634859387378951E-5 0.0 12-13 0.0 0.0 0.0 1.1444015711652657E-4 0.0 14-15 0.0 0.0 0.0 1.3078875099031609E-4 0.0 16-17 0.0 0.0 0.0 1.9618312648547411E-4 0.0 18-19 0.0 0.0 0.0 2.9427468972821114E-4 0.0 20-21 0.0 0.0 0.0 7.029895365729488E-4 0.0 22-23 0.0 0.0 0.0 0.00166755657512653 0.0 24-25 0.0 0.0 0.0 0.003841919560340535 0.0 26-27 0.0 0.0 0.0 0.00734051864933149 0.0 28-29 0.0 0.0 0.0 0.018882625924226883 0.0 30-31 0.0 0.0 0.0 0.05303483852657316 0.0 32-33 0.0 0.0 0.0 0.11870714011758562 0.0 34-35 0.0 0.0 0.0 0.21992128479021647 0.0 36-37 0.0 0.0 0.0 0.3772928494193143 0.0 38-39 0.0 0.0 0.0 0.6165054749806025 0.0 40-41 0.0 0.0 0.0 0.8834126185640899 0.0 42-43 0.0 0.0 0.0 1.1419819792719448 0.0 44-45 0.0 0.0 0.0 1.4363057147817773 0.0 46-47 0.0 0.0 0.0 1.7788087564376676 0.0 48-49 0.0 0.0 0.0 2.152570309580243 0.0 50-51 0.0 0.0 0.0 2.56305080456335 0.0 52-53 0.0 0.0 0.0 2.941095689300859 0.0 54-55 0.0 0.0 0.0 3.3315655053824473 0.0 56-57 0.0 0.0 0.0 3.7676969441535304 0.0 58-59 0.0 0.0 0.0 4.219228758353722 0.0 60-61 0.0 0.0 0.0 4.698569530733231 0.0 62-63 0.0 0.0 0.0 5.148875200392889 0.0 64-65 0.0 3.269718774757902E-5 0.0 5.591905745778711 0.0 66-67 0.0 3.269718774757902E-5 0.0 6.062483671841869 0.0 68-69 0.0 3.269718774757902E-5 0.0 6.599534980595854 0.0 70-71 0.0 3.269718774757902E-5 0.0 7.201195932339056 0.0 72-73 0.0 3.269718774757902E-5 0.0 7.8156087873038125 0.0 74-75 0.0 3.269718774757902E-5 0.0 8.413231636360187 0.0 76-77 0.0 3.269718774757902E-5 0.0 9.032254794797353 0.0 78-79 0.0 3.269718774757902E-5 0.0 9.722181804865144 0.0 80-81 0.0 3.269718774757902E-5 0.0 10.461775843121508 0.0 82-83 0.0 3.269718774757902E-5 0.0 11.20002929668022 0.0 84-85 0.0 3.269718774757902E-5 0.0 11.89507341663051 3.269718774757902E-5 86-87 0.0 3.269718774757902E-5 0.0 12.600727774004886 3.269718774757902E-5 88-89 0.0 4.904578162136853E-5 0.0 13.338278238027026 3.269718774757902E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13020 0.0 39.876816 1 GTATCAA 20745 0.0 35.230717 1 TATCAAC 26295 0.0 27.608776 2 ATCAACG 26320 0.0 27.505495 3 TCAACGC 26480 0.0 27.321384 4 CAACGCA 26925 0.0 26.905073 5 GTACATG 20620 0.0 26.307024 1 AACGCAG 27640 0.0 26.226246 6 TACATGG 20650 0.0 25.804094 2 ACATGGG 20840 0.0 24.585377 3 TAAGGTG 1525 0.0 24.575823 5 GCGTATC 120 1.7218909E-4 23.729258 1 ACGCAGA 31090 0.0 23.270191 7 CGCAGAG 31280 0.0 23.113676 8 GTAAGGT 1525 0.0 22.398218 4 CATGGGA 14665 0.0 21.965368 4 GAGTACT 17325 0.0 21.810387 12-13 GCAGAGT 32965 0.0 21.414143 9 ATGGGAT 5210 0.0 21.307327 5 CATGGGG 6035 0.0 21.303102 4 >>END_MODULE