##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939751_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 109988 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.13265992653744 31.0 31.0 33.0 30.0 34.0 2 31.224306287958687 31.0 31.0 34.0 28.0 34.0 3 31.14507946321417 31.0 31.0 34.0 28.0 34.0 4 35.02208422737026 35.0 35.0 37.0 32.0 37.0 5 34.83417281885296 35.0 35.0 37.0 32.0 37.0 6 35.00500963741499 36.0 35.0 37.0 32.0 37.0 7 34.84461941302688 35.0 35.0 37.0 32.0 37.0 8 34.88234171000473 36.0 35.0 37.0 32.0 37.0 9 36.25572789758883 38.0 35.0 39.0 32.0 39.0 10-11 36.19133450921919 38.0 35.0 39.0 31.0 39.0 12-13 36.130077826672 38.0 35.0 39.0 31.0 39.0 14-15 36.677273884423755 38.0 35.5 40.0 30.5 41.0 16-17 36.66823198894424 38.0 35.5 40.0 30.5 41.0 18-19 36.915431683456376 38.5 35.5 40.0 31.0 41.0 20-21 37.02374349929083 39.0 35.0 40.0 31.0 41.0 22-23 36.73168436556715 38.5 35.0 40.0 31.0 41.0 24-25 36.378323089791614 38.0 35.0 40.0 30.5 41.0 26-27 35.76639269738517 38.0 34.5 40.0 29.5 41.0 28-29 35.32302160235662 37.5 34.0 40.0 28.5 41.0 30-31 34.517315525330034 36.5 33.5 39.0 27.0 40.5 32-33 33.461536713095974 35.0 33.0 39.0 24.5 40.5 34-35 32.585332036222134 35.0 33.0 39.0 20.5 40.5 36-37 31.90282121686002 35.0 32.5 39.0 15.5 41.0 38-39 31.144374840891732 35.0 31.0 38.0 5.0 40.0 40-41 30.53093064697967 35.0 31.0 38.0 2.0 40.0 42-43 29.626027384805617 35.0 28.5 38.0 2.0 40.0 44-45 28.53600847365167 35.0 24.0 38.0 2.0 40.0 46-47 27.328022147870676 35.0 20.0 38.0 2.0 40.0 48-49 26.26535622067862 34.0 4.5 38.0 2.0 40.0 50-51 25.41219951267411 33.0 2.0 38.0 2.0 40.0 52-53 24.81884387387715 33.0 2.0 38.0 2.0 40.0 54-55 24.09772429719606 33.0 2.0 37.0 2.0 40.0 56-57 22.57008491835473 32.0 2.0 36.5 2.0 40.0 58-59 19.90253936793105 24.0 2.0 35.0 2.0 39.0 60-61 19.226111030294213 23.0 2.0 35.0 2.0 39.0 62-63 19.725829181365242 26.0 2.0 35.0 2.0 39.0 64-65 18.876659271920573 23.5 2.0 35.0 2.0 38.5 66-67 15.085900280030549 5.0 2.0 30.5 2.0 36.5 68-69 10.444189366112667 2.0 2.0 17.5 2.0 32.0 70-71 9.709959268283813 2.0 2.0 16.5 2.0 29.0 72-73 10.799555406044295 2.0 2.0 21.0 2.0 31.5 74-75 11.150161835836638 2.0 2.0 23.0 2.0 32.5 76-77 11.566039022438812 2.0 2.0 25.0 2.0 32.5 78-79 13.151984761974033 2.0 2.0 29.5 2.0 34.0 80-81 14.378409462850492 2.0 2.0 31.5 2.0 35.0 82-83 14.73671673273448 2.0 2.0 33.0 2.0 35.0 84-85 14.66174037167691 2.0 2.0 33.0 2.0 35.0 86-87 14.480320580426955 2.0 2.0 33.0 2.0 35.0 88-89 14.172296068662035 2.0 2.0 33.0 2.0 35.0 90-91 13.318016510892097 2.0 2.0 31.5 2.0 35.0 92-93 12.251859293741134 2.0 2.0 30.5 2.0 34.5 94-95 10.025198203440375 2.0 2.0 23.0 2.0 34.0 96-97 9.337786849474488 2.0 2.0 15.0 2.0 33.5 98-99 8.767615558060879 2.0 2.0 2.0 2.0 33.0 100-101 8.473019783976433 2.0 2.0 2.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.23168322377513206 1101 2 0.41839378348306155 1101 3 0.5184096534322506 1101 4 0.6371370253971804 1101 5 0.06523185994828395 1101 6 0.4310592447487167 1101 7 0.15063904106958148 1101 8 -0.06055347496223362 1101 9 1.076920554527753 1101 10-11 0.9037971417730333 1101 12-13 1.1656854763757494 1101 14-15 0.6902730311955168 1101 16-17 1.0694235194287103 1101 18-19 0.7202066979909034 1101 20-21 0.8860441430570987 1101 22-23 1.4610542035021297 1101 24-25 1.571149142964515 1101 26-27 2.023397900750922 1101 28-29 1.467954744683297 1101 30-31 1.4957811424500704 1101 32-33 1.3098099564806063 1101 34-35 0.9730022968644008 1101 36-37 1.9231584291950021 1101 38-39 2.6109457730009495 1101 40-41 1.3513940437672645 1101 42-43 1.462255740516067 1101 44-45 -0.5676432778435547 1101 46-47 -0.23434792240938052 1101 48-49 -0.2702710947471907 1101 50-51 -0.31553405375025534 1101 52-53 0.0895150402755931 1101 54-55 -0.19903490469399188 1101 56-57 -0.6077794234786076 1101 58-59 -6.986078206156041 1101 60-61 -7.143152523341108 1101 62-63 -4.278524728416649 1101 64-65 -2.470446297320926 1101 66-67 -3.5971645615480092 1101 68-69 -3.1743259837866757 1101 70-71 -3.0779574755415666 1101 72-73 -4.7735984540834195 1101 74-75 -4.832493377258002 1101 76-77 -6.063141424128201 1101 78-79 -7.6513121845427605 1101 80-81 -6.664180873401127 1101 82-83 -4.786552198953471 1101 84-85 -4.360887084306347 1101 86-87 -3.681785364135644 1101 88-89 -3.9945809958977296 1101 90-91 -6.021406258447316 1101 92-93 -7.6533970856422275 1101 94-95 -6.358954770354485 1101 96-97 -6.547511270038979 1101 98-99 -6.26699838487281 1101 100-101 -5.903676888737434 1102 1 0.3912070332989437 1102 2 0.6112509263402046 1102 3 0.9422191772417747 1102 4 0.8442798825400359 1102 5 0.4557080504244766 1102 6 0.7858211495106247 1102 7 1.0530199934505333 1102 8 0.6632560488472876 1102 9 1.2150157926229852 1102 10-11 0.7514161893920814 1102 12-13 0.8299711906614604 1102 14-15 0.8831301740526598 1102 16-17 1.3956139956191862 1102 18-19 0.9785400313242363 1102 20-21 0.7836631906761511 1102 22-23 0.3931970606449937 1102 24-25 0.580672952488321 1102 26-27 1.0995883769413979 1102 28-29 0.09652617325471624 1102 30-31 0.1695906662595874 1102 32-33 0.7550480517187026 1102 34-35 0.9956213444834461 1102 36-37 0.662444143480716 1102 38-39 1.1954695825247583 1102 40-41 1.3335369009101221 1102 42-43 1.3146366928970181 1102 44-45 0.6478329126326372 1102 46-47 0.8454139823525253 1102 48-49 1.572586048109951 1102 50-51 1.7094659462497432 1102 52-53 1.4799912307517857 1102 54-55 1.4081079524488622 1102 56-57 1.0053158146166332 1102 58-59 -6.363459158536994 1102 60-61 -6.512200142388725 1102 62-63 -2.2987628236547444 1102 64-65 -0.7335415354161654 1102 66-67 -0.9162121805956289 1102 68-69 -0.7767069361676278 1102 70-71 -1.1279574755415673 1102 72-73 -1.7438365493215144 1102 74-75 -3.1301124248770495 1102 76-77 -3.7345699955567717 1102 78-79 -5.589407422637999 1102 80-81 -3.4737046829249376 1102 82-83 -0.4151236275248973 1102 84-85 0.184351010931751 1102 86-87 0.5086908263405459 1102 88-89 0.9435142421975069 1102 90-91 -2.709501496542554 1102 92-93 -7.073635180880323 1102 94-95 -5.543478579878293 1102 96-97 -4.871320793848502 1102 98-99 -4.595569813444239 1102 100-101 -4.517962603023149 1103 1 0.40560179883874525 1103 2 0.340912867452527 1103 3 0.4652289918546444 1103 4 0.5520225215803904 1103 5 0.3207025978617679 1103 6 0.02965975365457041 1103 7 0.2665423489576213 1103 8 0.09084601613191268 1103 9 0.499312412034115 1103 10-11 0.32529841403766113 1103 12-13 0.3278992168337638 1103 14-15 0.2982882983710837 1103 16-17 0.5054286084872359 1103 18-19 -0.005047754935304738 1103 20-21 -0.0035232869683454737 1103 22-23 0.782237409609003 1103 24-25 0.34048756535637636 1103 26-27 0.31398314248120585 1103 28-29 1.3557410042252798 1103 30-31 1.1506157480480255 1103 32-33 0.817697997193072 1103 34-35 1.3408776149305552 1103 36-37 0.9428149177446237 1103 38-39 1.8419254167668555 1103 40-41 2.477157402545892 1103 42-43 2.7884643919155607 1103 44-45 2.9651175364058098 1103 46-47 3.140779304053723 1103 48-49 3.242324325100139 1103 50-51 3.546107167623788 1103 52-53 2.2820086789524368 1103 54-55 2.0940948662983736 1103 56-57 2.4457193042567624 1103 58-59 -2.7563708270211826 1103 60-61 -3.736027841407264 1103 62-63 0.9057628033899654 1103 64-65 2.030253448226148 1103 66-67 0.9149219524469032 1103 68-69 -0.801679673354105 1103 70-71 -1.1611635824117972 1103 72-73 -2.5484712276203147 1103 74-75 -2.4524679319653817 1103 76-77 -3.578026920311407 1103 78-79 -5.269887248155992 1103 80-81 -2.421687234724283 1103 82-83 0.5802416941762996 1103 84-85 0.6874589716732977 1103 86-87 0.4820823203427267 1103 88-89 0.7033833806926015 1103 90-91 -2.142653077785738 1103 92-93 -5.83552176757607 1103 94-95 -5.2899344141203875 1103 96-97 -5.768376409988088 1103 98-99 -5.744097621514031 1103 100-101 -5.64031811011148 1104 1 0.4544104965024047 1104 2 0.41990893499821524 1104 3 0.4456823807049801 1104 4 0.5401673284274864 1104 5 0.23644398116040577 1104 6 0.5068168205062946 1104 7 0.4097299501604894 1104 8 0.6061131917044307 1104 9 0.3647993424065419 1104 10-11 0.4007668387427259 1104 12-13 0.25659456728484287 1104 14-15 0.3895154554379374 1104 16-17 0.6065447315499171 1104 18-19 0.6209642737484771 1104 20-21 0.45498353699649385 1104 22-23 0.30499359744152343 1104 24-25 1.0476642944796666 1104 26-27 1.0559736583266783 1104 28-29 0.5558335325620831 1104 30-31 0.6692659909349103 1104 32-33 0.9946584413290935 1104 34-35 1.0510325998947039 1104 36-37 1.4754311564677316 1104 38-39 0.49049122754640706 1104 40-41 0.7923031346763558 1104 42-43 0.43043755869788214 1104 44-45 0.4929627827625076 1104 46-47 0.5209551078936485 1104 48-49 0.019880420404323473 1104 50-51 -0.592049205265404 1104 52-53 -0.602909202148652 1104 54-55 -1.147519753178841 1104 56-57 -0.48580972650890786 1104 58-59 -4.265623660701497 1104 60-61 -5.662091917280501 1104 62-63 -1.7504944253863464 1104 64-65 -0.6477190245936555 1104 66-67 -1.9153463797298258 1104 68-69 -2.8197805292412212 1104 70-71 -2.0915938391779303 1104 72-73 -3.838749969234935 1104 74-75 -4.227190346954972 1104 76-77 -4.588898999885776 1104 78-79 -6.283130366360943 1104 80-81 -4.261150570370825 1104 82-83 -2.831249168650439 1104 84-85 -2.28134162976089 1104 86-87 -2.385573242923522 1104 88-89 -3.0915506928674272 1104 90-91 -5.669891106932164 1104 92-93 -7.746578903824045 1104 94-95 -6.169560830960545 1104 96-97 -5.886147633675343 1104 98-99 -5.33139232426675 1104 100-101 -4.91504052510107 1105 1 -0.2795570863023862 1105 2 -0.9292806351215894 1105 3 -0.370737633389453 1105 4 -0.8151885559981693 1105 5 -0.8130627136951247 1105 6 -0.6600260265691134 1105 7 -0.36757801319398453 1105 8 0.0596015637974574 1105 9 -0.9075755695032584 1105 10-11 -0.6431020830331704 1105 12-13 -0.16570987246146274 1105 14-15 -1.0085641781068162 1105 16-17 -0.6007315193309779 1105 18-19 -1.2784367128618115 1105 20-21 -1.189924849190966 1105 22-23 -0.8767364941722917 1105 24-25 -0.1552074461827715 1105 26-27 -0.23745481242737299 1105 28-29 -1.9064638599678716 1105 30-31 -1.346854516464667 1105 32-33 0.0543835998914588 1105 34-35 0.39528911856982774 1105 36-37 0.4051351733810513 1105 38-39 0.5863333699001743 1105 40-41 0.3612777646974976 1105 42-43 0.8021782211362236 1105 44-45 0.34999238107117847 1105 46-47 0.8646830853425556 1105 48-49 1.8739149517644371 1105 50-51 2.6815589695055593 1105 52-53 2.3507553503531113 1105 54-55 1.695151141817636 1105 56-57 1.7571430571415512 1105 58-59 -5.268442547241312 1105 60-61 -5.912532368302347 1105 62-63 -1.5922844183391298 1105 64-65 -0.11908970817363951 1105 66-67 -0.44793975534645725 1105 68-69 -2.5572717202207844 1105 70-71 -1.9547016615880786 1105 72-73 -2.9350325626105516 1105 74-75 -4.012338338498312 1105 76-77 -4.70500188924448 1105 78-79 -6.111002107023381 1105 80-81 -4.038987074951516 1105 82-83 -2.7772498733720763 1105 84-85 -2.734918092058283 1105 86-87 -2.327521798244172 1105 88-89 -2.3674492129519944 1105 90-91 -3.8717938553465405 1105 92-93 -5.019482356960056 1105 94-95 -5.045970274230453 1105 96-97 -5.756038401821924 1105 98-99 -5.906533268593741 1105 100-101 -5.5548396794351085 1106 1 0.23658518455944488 1106 2 0.3330996658360057 1106 3 -0.491394268136375 1106 4 -0.036392386367523955 1106 5 -0.15437598318897017 1106 6 -0.25619565721206783 1106 7 -0.24445899814610783 1106 8 0.34140730935148866 1106 9 0.3788813388414738 1106 10-11 -0.3363989366583411 1106 12-13 -0.1960792295066014 1106 14-15 0.1167436194308138 1106 16-17 -0.09430197076737556 1106 18-19 0.022657678383055213 1106 20-21 -0.6394460530213308 1106 22-23 -1.0139457964978646 1106 24-25 -0.6945371315452888 1106 26-27 -0.8138570012098612 1106 28-29 -0.4849864317872914 1106 30-31 -1.375787485000913 1106 32-33 -0.8117586709703701 1106 34-35 -0.5186643698022664 1106 36-37 -1.1930180413932305 1106 38-39 -1.0650346191559095 1106 40-41 -0.2941941915268522 1106 42-43 0.37009887777097106 1106 44-45 1.1348077025486027 1106 46-47 1.4886912932768936 1106 48-49 1.6503171405469281 1106 50-51 2.3035835933085664 1106 52-53 1.237554255961868 1106 54-55 1.389200389423653 1106 56-57 1.8544754784821755 1106 58-59 -4.183627225763884 1106 60-61 -4.717662327262676 1106 62-63 -0.7201913950833152 1106 64-65 -0.12681884634053375 1106 66-67 -0.5157920125284008 1106 68-69 -1.290992650453342 1106 70-71 -1.9838398284827434 1106 72-73 -3.5770298266324394 1106 74-75 -3.292297298826629 1106 76-77 -4.419023777069377 1106 78-79 -6.576802380621192 1106 80-81 -3.8073181283030877 1106 82-83 -2.165963963659353 1106 84-85 -2.0589262999926206 1106 86-87 -1.9719814425670172 1106 88-89 -2.374973152760475 1106 90-91 -5.179249395702218 1106 92-93 -6.265651987603011 1106 94-95 -6.282974378197622 1106 96-97 -6.014177936705646 1106 98-99 -5.437586620166928 1106 100-101 -5.403676888737434 1107 1 0.07187930220650429 1107 2 -0.031606216516937735 1107 3 0.4615469083342134 1107 4 -0.1422747393087036 1107 5 0.645624016811027 1107 6 0.33203963690558425 1107 7 0.17907041361860365 1107 8 0.6708190740573698 1107 9 0.8612342800179462 1107 10-11 0.22830694569459808 1107 12-13 0.5745090057875117 1107 14-15 0.4108612664896327 1107 16-17 -0.4884196178261959 1107 18-19 0.10501061955952906 1107 20-21 0.3429068881551416 1107 22-23 0.9566424387962513 1107 24-25 0.47605110374882287 1107 26-27 0.39790770467249104 1107 28-29 0.33854297997741156 1107 30-31 0.1183301620579087 1107 32-33 0.1470648584413894 1107 34-35 0.7931003360800872 1107 36-37 0.6187466644891266 1107 38-39 0.1879065573146761 1107 40-41 0.40580580847314707 1107 42-43 1.0524518189474392 1107 44-45 1.0759841731368418 1107 46-47 0.2122207050416023 1107 48-49 0.6620818464292775 1107 50-51 1.4624071227203324 1107 52-53 1.6669660206677506 1107 54-55 1.3362592129530633 1107 56-57 2.0662401843645313 1107 58-59 -3.2895095787050614 1107 60-61 -5.670603503733265 1107 62-63 -0.37901492449507757 1107 64-65 1.0672988007182909 1107 66-67 -1.1040273066460458 1107 68-69 -3.055698532806284 1107 70-71 -2.6308986520121547 1107 72-73 -3.677029826632439 1107 74-75 -2.7217090635325114 1107 76-77 -4.536670835892906 1107 78-79 -7.265037674738839 1107 80-81 -4.860259304773676 1107 82-83 -2.401258081306411 1107 84-85 -1.8471615941102666 1107 86-87 -1.6131579131552538 1107 88-89 -1.780855505701652 1107 90-91 -3.7792493957022177 1107 92-93 -5.412710811132424 1107 94-95 -5.482974378197621 1107 96-97 -5.831824995529175 1107 98-99 -4.878763090755163 1107 100-101 -4.738971006384492 1108 1 -0.002889778979689339 1108 2 -0.2150772908971028 1108 3 -0.4289732666779429 1108 4 -0.20294561923091692 1108 5 -0.4377984430820163 1108 6 -0.13436775249645905 1108 7 0.3739172779841837 1108 8 0.0069396379854254064 1108 9 0.4460665600373943 1108 10-11 0.4276263428749587 1108 12-13 0.47353671604516734 1108 14-15 0.5675457584682491 1108 16-17 0.05110396020005936 1108 18-19 -0.03261699346915492 1108 20-21 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2311 9 -0.6613714564915298 2311 10-11 0.014403202379092761 2311 12-13 -0.33224840792177446 2311 14-15 -1.1101401919449856 2311 16-17 -0.21749108112225457 2311 18-19 -0.4293112083451831 2311 20-21 -0.4227712839401505 2311 22-23 -0.29245819319208266 2311 24-25 -0.8865643556580736 2311 26-27 -1.1234340551719413 2311 28-29 -0.7279130239117464 2311 30-31 -0.6148249181559962 2311 32-33 -0.5549283355304127 2311 34-35 0.2507571178010366 2311 36-37 -0.5948167774165647 2311 38-39 -0.0639164859522161 2311 40-41 -0.3409613281335595 2311 42-43 -1.3099205680238768 2311 44-45 -0.8386487874854254 2311 46-47 -1.1222955808115849 2311 48-49 -1.0293620038380986 2311 50-51 -0.9426001694527315 2311 52-53 -1.1965731139943792 2311 54-55 -0.4395307724625859 2311 56-57 -0.1979309386301189 2311 58-59 1.646222069326054 2311 60-61 1.7590513334082054 2311 62-63 0.2508829850819758 2311 64-65 0.4097878624586855 2311 66-67 -0.002811944468119165 2311 68-69 -1.1703314934285487 2311 70-71 -0.02341202099611195 2311 72-73 -0.6049345422376895 2311 74-75 0.7511154381689966 2311 76-77 2.218263534549486 2311 78-79 1.977888917385613 2311 80-81 1.1245243607586524 2311 82-83 0.5485717679886779 2311 84-85 0.5867713178975098 2311 86-87 0.44328350638777003 2311 88-89 1.048945174901169 2311 90-91 1.6338279013322978 2311 92-93 1.834688313806808 2311 94-95 3.4340893067805016 2311 96-97 4.028797269905924 2311 98-99 4.018125582069338 2311 100-101 3.6293809625022346 2312 1 -0.168179035178035 2312 2 0.11550122149959208 2312 3 -0.10665921709116333 2312 4 0.15242628159553107 2312 5 -0.10721993068532498 2312 6 -0.23354130621547142 2312 7 -0.11368602780094506 2312 8 0.08131980327468114 2312 9 -0.6117846796320308 2312 10-11 -0.2665885331580995 2312 12-13 -0.39423187899614476 2312 14-15 -0.7341071340937404 2312 16-17 -0.8125324034363075 2312 18-19 -0.18550955545262582 2312 20-21 -0.19136632526246444 2312 22-23 0.12489717870874273 2312 24-25 0.05145217326754192 2312 26-27 0.21540892003466894 2312 28-29 0.11919441410478271 2312 30-31 0.5339354124225153 2312 32-33 -0.1706308148692557 2312 34-35 0.06480670457789728 2312 36-37 0.3101419002693895 2312 38-39 0.4939347537172054 2312 40-41 0.6425097462466027 2312 42-43 0.6033025724719892 2312 44-45 0.1282933612749062 2312 46-47 0.5182002869570113 2312 48-49 0.31361320277347104 2312 50-51 0.04500313633239017 2312 52-53 0.6546665554271094 2312 54-55 0.4778245994382395 2312 56-57 1.045870714262442 2312 58-59 3.8734947965987807 2312 60-61 2.7879769532429144 2312 62-63 1.734354059462138 2312 64-65 0.6411928211363715 2312 66-67 1.2575186340442777 2312 68-69 -0.23644719590788732 2312 70-71 0.6749350864419039 2312 72-73 4.1471315734647884 2312 74-75 2.9164046943673423 2312 76-77 2.937271799012297 2312 78-79 3.4200376777161914 2312 80-81 2.5873342781140263 2312 82-83 1.5403073051787608 2312 84-85 1.7851184253355274 2312 86-87 1.6953496220902498 2312 88-89 1.7555567451491019 2312 90-91 2.3693650914149433 2312 92-93 3.095018892319205 2312 94-95 5.434089306780502 2312 96-97 6.504003881476173 2312 98-99 6.071844590333802 2312 100-101 4.9310338550642205 2313 1 0.058876206231271766 2313 2 0.031551678219905455 2313 3 0.12191842536207531 2313 4 -0.3374243781115922 2313 5 -0.3189786663675065 2313 6 0.031936437731175715 2313 7 -0.47304516945673214 2313 8 -0.38511487847100767 2313 9 -0.23009698933189782 2313 10-11 0.164323457562503 2313 12-13 -0.11501627801021641 2313 14-15 -0.2342883723132516 2313 16-17 0.1334586071480075 2313 18-19 0.0846803822014266 2313 20-21 -0.1867628744867602 2313 22-23 -0.042893164918922366 2313 24-25 0.012377213139956211 2313 26-27 0.1339242165403931 2313 28-29 0.19777930608680094 2313 30-31 0.5115706161342786 2313 32-33 0.24665206174376664 2313 34-35 0.8927391389696666 2313 36-37 1.2042987800721967 2313 38-39 1.128051036158844 2313 40-41 0.28692035955673845 2313 42-43 1.4719048633230827 2313 44-45 2.2854268975950447 2313 46-47 2.898546814432727 2313 48-49 2.2525359227966675 2313 50-51 2.3357817357234296 2313 52-53 2.4333746893983985 2313 54-55 2.3457019374112704 2313 56-57 3.3172205765213967 2313 58-59 5.0397989868264155 2313 60-61 5.216496599465909 2313 62-63 3.244720885618438 2313 64-65 3.884378264082585 2313 66-67 3.0431863156449737 2313 68-69 1.3923406828799898 2313 70-71 3.179937261300539 2313 72-73 5.48999803714465 2313 74-75 6.468383815724453 2313 76-77 6.61405155832794 2313 78-79 5.631143955808113 2313 80-81 4.308626144142734 2313 82-83 3.3818688536781085 2313 84-85 3.4549023893778656 2313 86-87 2.920846214811723 2313 88-89 3.4299804076110387 2313 90-91 5.042628829271983 2313 92-93 5.356690633656017 2313 94-95 6.928325931399904 2313 96-97 6.9621378527680395 2313 98-99 6.961071790565786 2313 100-101 6.574393286701162 2314 1 -0.42942788733597936 2314 2 -0.1853099202206394 2314 3 -0.16121997619737982 2314 4 -0.1517518634917039 2314 5 -0.6532866585702379 2314 6 -0.4485703848809095 2314 7 -1.0178794774489361 2314 8 -0.7457386601474241 2314 9 -0.6915979639907661 2314 10-11 -0.7174991545232672 2314 12-13 -0.7972774865872125 2314 14-15 -1.1180603021378133 2314 16-17 -0.9389098139046226 2314 18-19 -0.3992377464535437 2314 20-21 -0.26765956064660656 2314 22-23 -0.6630198705719437 2314 24-25 -0.1732953014799321 2314 26-27 -0.6136391362861104 2314 28-29 -1.6533415516129963 2314 30-31 -1.0171818205129028 2314 32-33 -1.4300048583342075 2314 34-35 -1.1408865920244864 2314 36-37 -1.1314712004346248 2314 38-39 -1.352943115887939 2314 40-41 -2.192124474751253 2314 42-43 -1.818299815039488 2314 44-45 -1.8407914259917 2314 46-47 -1.7084219964834517 2314 48-49 -2.953604428080528 2314 50-51 -3.824793313009515 2314 52-53 -3.707707181946631 2314 54-55 -3.1555163861754743 2314 56-57 -2.446668312367496 2314 58-59 -0.4314485765264102 2314 60-61 -1.439448819637402 2314 62-63 -2.8174136173055366 2314 64-65 -3.1010018528764824 2314 66-67 -1.5508682652517116 2314 68-69 -1.6863630208237126 2314 70-71 -0.8761056236897158 2314 72-73 1.185660805175841 2314 74-75 1.7702844005197758 2314 76-77 1.0748215388347617 2314 78-79 0.23202114879057234 2314 80-81 -1.4067734659937212 2314 82-83 -1.7874781248793958 2314 84-85 -2.106257454676717 2314 86-87 -2.303081660431941 2314 88-89 -1.436247662564396 2314 90-91 -0.7380729251139826 2314 92-93 0.34012143287629115 2314 94-95 2.20586004446033 2314 96-97 2.695081322553614 2314 98-99 2.793186800312375 2314 100-101 2.4852120001514555 2315 1 -0.703051978717081 2315 2 -0.4932884595075926 2315 3 -0.7784750817702424 2315 4 -0.723143348434597 2315 5 -0.3808740590548325 2315 6 -0.836074711637572 2315 7 -0.879423264226368 2315 8 -0.7443540980151937 2315 9 -0.7797772647868939 2315 10-11 -0.5446452258282122 2315 12-13 -0.4908565796990203 2315 14-15 -0.8746802398325215 2315 16-17 -1.0397665117239399 2315 18-19 -1.3060394079280968 2315 20-21 -1.9198748600581723 2315 22-23 -1.5223570114511347 2315 24-25 -1.639286994107131 2315 26-27 -1.3320538126447161 2315 28-29 -1.6853162833540836 2315 30-31 -1.4015708824720576 2315 32-33 -1.65997197498357 2315 34-35 -1.675673715596659 2315 36-37 -1.999505122206866 2315 38-39 -1.2903782145379878 2315 40-41 -1.3254751151112387 2315 42-43 -0.8589280601069831 2315 44-45 -0.5368800379681637 2315 46-47 -0.38629496290782583 2315 48-49 -1.2744767022238257 2315 50-51 -1.0269826518810987 2315 52-53 -0.7140675142415418 2315 54-55 -0.5280068673108147 2315 56-57 0.8250087385151623 2315 58-59 4.134404659887572 2315 60-61 3.57491601248444 2315 62-63 1.9900889787484637 2315 64-65 2.3836814285357733 2315 66-67 1.4550161238102461 2315 68-69 2.879100807490584 2315 70-71 2.23699579548647 2315 72-73 4.98520216896954 2315 74-75 5.531836840810536 2315 76-77 4.968166987086754 2315 78-79 3.9166005880429076 2315 80-81 2.2130776935770644 2315 82-83 0.9223263057194266 2315 84-85 0.6235365917061149 2315 86-87 0.9655666607864735 2315 88-89 1.4169454838530484 2315 90-91 2.219871000119662 2315 92-93 3.007739986008863 2315 94-95 5.192836506904083 2315 96-97 5.4194669231074375 2315 98-99 5.789076381482332 2315 100-101 4.708472643972846 2316 1 -0.396748148773888 2316 2 -0.2708219027914467 2316 3 -0.9090413269599047 2316 4 -0.31678454323026983 2316 5 -1.3504544145615185 2316 6 -0.7012936964277543 2316 7 -0.14641978245983012 2316 8 -0.8252593573151756 2316 9 -1.423079445472247 2316 10-11 -0.8109087405799045 2316 12-13 -1.221569425585038 2316 14-15 -0.5812955962554653 2316 16-17 -0.6325372648850163 2316 18-19 -0.6361658510287072 2316 20-21 -1.0492499745899622 2316 22-23 -0.7237497180664931 2316 24-25 -2.606301837427644 2316 26-27 -0.6991511188569177 2316 28-29 -0.5604766278657252 2316 30-31 -0.06892473990287584 2316 32-33 0.17157466236296415 2316 34-35 -0.34023299725324563 2316 36-37 0.11580548801853396 2316 38-39 0.040847733785263074 2316 40-41 0.19404110259079488 2316 42-43 0.6612753483592044 2316 44-45 -0.3397021013729642 2316 46-47 0.03967168543375976 2316 48-49 0.3944347876057499 2316 50-51 1.4957404560536673 2316 52-53 0.5561817069422617 2316 54-55 0.6372396051099294 2316 56-57 1.3613382235802156 2316 58-59 3.75754924482435 2316 60-61 3.822533751168695 2316 62-63 2.2356909578578623 2316 64-65 2.018279192875152 2316 66-67 5.177345242373562 2316 68-69 6.706066173076071 2316 70-71 4.262238602889806 2316 72-73 5.767087820426385 2316 74-75 5.916526230585136 2316 76-77 5.565289948420819 2316 78-79 4.349668207614101 2316 80-81 2.6024857932655383 2316 82-83 2.6458007422230008 2316 84-85 2.0606815431446375 2316 86-87 2.1329205182172952 2316 88-89 2.0907131217493298 2316 90-91 2.6393780552781756 2316 92-93 3.6157205614165964 2316 94-95 5.672907974743556 2316 96-97 5.906410298588472 2316 98-99 6.375158477872288 2316 100-101 5.679656444595898 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 1.0 3 3.0 4 9.0 5 34.0 6 90.0 7 255.0 8 456.0 9 815.0 10 1326.0 11 2033.0 12 2870.0 13 3404.0 14 3877.0 15 4696.0 16 5546.0 17 6096.0 18 5479.0 19 4531.0 20 4444.0 21 4508.0 22 3817.0 23 3634.0 24 3306.0 25 2551.0 26 2177.0 27 2617.0 28 3427.0 29 4014.0 30 4625.0 31 4854.0 32 4958.0 33 5022.0 34 4946.0 35 4457.0 36 3055.0 37 1639.0 38 401.0 39 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.69285824716486 23.64153911912622 15.263871079220436 14.401731554488492 2 15.190805684618253 27.442921959247506 37.887453287386 19.478819068748237 3 20.86212540005819 36.13525603724178 32.314700320046555 10.687918242653478 4 10.358402734843802 25.73007964505219 30.85973015238026 33.051787467723756 5 7.091134035623965 45.798992049196734 31.49573349344104 15.61414042173826 6 20.42859221005928 40.92264610684802 14.95435865730807 23.69440302578463 7 15.012546823289814 44.61032112594101 19.350292759210095 21.026839291559078 8 25.664617958322726 33.67276430156017 19.514856166127213 21.14776157398989 9 23.54438666036295 19.244826708368183 20.131287049496308 37.07949958177256 10-11 21.49098083427283 32.65992653744045 22.58382732661745 23.26526530166927 12-13 27.172055133287266 31.695275848274356 25.44868531112485 15.683983707313526 14-15 22.81839837073135 30.99383569116631 29.070444048441647 17.11732188966069 16-17 14.318380186929483 36.94084809251918 29.34729243190166 19.39347928864967 18-19 13.970741996781438 31.86649331284606 37.28622473565058 16.876539954721924 20-21 15.811724915445321 30.130105102374806 37.10495690438957 16.953213077790306 22-23 14.082899952722114 34.21873295268575 36.87675019092992 14.82161690366222 24-25 14.880259664690692 35.53887696839656 36.87675019092992 12.704113175982833 26-27 14.540288669166952 34.50255710876236 38.653483350380725 12.303670871689965 28-29 12.99932264414269 33.92098121133048 39.83943483973033 13.240261304796498 30-31 14.296105690982989 35.76299542648276 37.65150344150353 12.289395441030724 32-33 12.590067053074213 34.39254460734174 39.68087282645755 13.336515513126493 34-35 10.396730507835054 36.00032731289749 40.36404467821051 13.238897501056949 36-37 12.53222513719838 35.0395795159523 38.606510046058645 13.821685300790682 38-39 11.978884862483348 32.638892046358734 39.9450751806199 15.437147910538018 40-41 11.175020915936125 33.22269106252955 40.2804554217744 15.321832599759922 42-43 12.97418712402639 32.44365226959609 39.781929622741885 14.800230983635629 44-45 11.292684035829582 33.16873550675215 39.51757377347338 16.02100668394489 46-47 13.655750675239403 31.479342676039686 37.48328952992425 17.38161711879666 48-49 13.026555110949436 30.799381593306656 36.92115314659876 19.252910149145144 50-51 11.165924787552798 30.535107780864518 38.64106539599976 19.657902035582918 52-53 12.058743293625534 30.619714467582064 38.71965081385832 18.601891424934074 54-55 11.608230737056502 29.592736729164017 36.81690883631272 21.982123697466765 56-57 14.816231203135885 29.762213693541117 33.86599651264961 21.55555859067339 58-59 13.618905317578903 30.60380783016014 35.90076174936521 19.876525102895748 60-61 14.50056546641859 32.86591149538506 31.88318558243041 20.750337455765933 62-63 11.69769291964996 34.47164450505739 32.00318217979316 21.82748039549949 64-65 12.060322100345596 33.7932164956903 31.889027005614217 22.257434398349886 66-67 16.758033852003617 30.20285360946527 30.365745611606403 22.67336692692471 68-69 20.100210995139157 27.67825814389775 29.636629878970194 22.5849009819929 70-71 19.2098517308918 27.203915383792282 30.596844803602746 22.989388081713166 72-73 18.694436328728244 26.366338791732176 30.857808014617564 24.08141686492202 74-75 18.163161543794775 26.34157614975734 30.830598567136587 24.6646637393113 76-77 15.631697557834705 26.809547900473717 31.86036885434393 25.698385687347653 78-79 14.661899608681159 27.24026611507605 31.933352511152812 26.164481765089974 80-81 14.573019661325151 27.120809182861688 31.5417661097852 26.764405046027957 82-83 14.458850056369785 27.307979052260244 30.64516129032258 27.588009601047386 84-85 14.449758155435138 27.399352656653452 30.142833763683313 28.008055424228097 86-87 15.16895562556542 27.371129568257345 29.593260868576937 27.8666539376003 88-89 15.100283667309162 27.265701712914137 28.959977452085685 28.67403716769102 90-91 15.91796563523778 26.020075728234964 29.53537857282805 28.526580063699203 92-93 15.976390647344404 25.94296228150874 29.581371200597523 28.499275870549333 94-95 17.553772484894854 24.449831550518713 30.361002677678485 27.63539328690795 96-97 16.86182346028893 24.619248404414645 30.65966221976452 27.8592659155319 98-99 16.75946599042605 25.265764399974273 29.778935472311808 28.19583413728787 100-101 15.23042158362055 25.94508228527082 29.851863687201845 28.972632443906782 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1437.0 1 965.0 2 477.0 3 449.5 4 414.0 5 404.0 6 487.5 7 658.0 8 803.5 9 826.5 10 763.5 11 728.0 12 732.0 13 716.5 14 692.5 15 695.0 16 699.0 17 697.0 18 714.0 19 760.5 20 823.5 21 914.0 22 1056.5 23 1226.0 24 1346.5 25 1540.0 26 1796.5 27 1990.0 28 2206.0 29 2418.5 30 2649.5 31 2912.5 32 3047.0 33 3100.0 34 3245.5 35 3310.0 36 3216.0 37 3050.0 38 2824.0 39 2614.0 40 2453.5 41 2350.5 42 2305.5 43 2430.0 44 2633.5 45 2869.0 46 3313.5 47 4020.5 48 4642.5 49 4878.5 50 4753.5 51 3983.0 52 2698.5 53 1620.5 54 1086.0 55 827.0 56 648.5 57 568.5 58 515.0 59 460.0 60 407.5 61 369.0 62 334.5 63 286.0 64 226.5 65 172.0 66 136.0 67 94.5 68 61.5 69 45.0 70 35.5 71 23.5 72 13.5 73 10.5 74 8.5 75 6.0 76 5.5 77 3.5 78 1.5 79 1.0 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026366512710477505 2 0.006364330654253192 3 0.0036367603738589665 4 0.0 5 0.05636978579481398 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 9.091900934647416E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.545950467323708E-4 28-29 0.0013637851401971126 30-31 0.006364330654253192 32-33 4.545950467323708E-4 34-35 0.0013637851401971126 36-37 0.017729206822562463 38-39 0.017729206822562463 40-41 0.0218205622431538 42-43 0.021365967196421427 44-45 0.020911372149689057 46-47 0.024548132523548025 48-49 0.025457322617012768 50-51 0.017729206822562463 52-53 0.01636542168236535 54-55 0.009091900934647415 56-57 0.14774339018802052 58-59 0.8155435138378733 60-61 0.3127613921518711 62-63 4.545950467323708E-4 64-65 0.16138124158999165 66-67 1.4856166127213877 68-69 2.830763356002473 70-71 2.286158490017093 72-73 1.9752154780521514 74-75 1.6483616394515768 76-77 1.157398988980616 78-79 0.44232098047059676 80-81 4.545950467323708E-4 82-83 0.0 84-85 0.0 86-87 0.003182165327126596 88-89 0.0 90-91 0.23047968869331198 92-93 0.18274720878641307 94-95 1.3624213550569153 96-97 1.3506018838418736 98-99 1.0464777975779176 100-101 0.4186820380405135 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 109988.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.962977779394116 #Duplication Level Percentage of deduplicated Percentage of total 1 82.13384621747942 36.10848456195222 2 8.493609628986226 7.468087427719388 3 3.2944534061297928 4.345019456668 4 1.747528642925094 3.0730625159108267 5 1.0154278860073624 2.2320616794559407 6 0.7341688381519627 1.9365748990798997 7 0.4467055465938702 1.3746954213186895 8 0.40120775944079085 1.4110630250572789 9 0.2523059105761674 0.9982907226242862 >10 1.2677337965835298 9.960177473906244 >50 0.08272324936923522 2.5193657489907992 >100 0.10340406171154404 7.891770011273957 >500 0.012408487405385284 3.8313270538604214 >1k 0.014476568639616163 16.850020002182056 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3697 3.36127577553915 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3510 3.191257228061243 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 3225 2.9321380514237916 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3097 2.815761719460305 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2406 2.187511364876168 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 1584 1.4401571080481508 TruSeq Adapter, Index 1 (95% over 21bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1014 0.9219187547732479 No Hit ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT 863 0.784631050660072 RNA PCR Primer, Index 1 (95% over 23bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 855 0.7773575299123541 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 757 0.6882569007528094 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 604 0.5491508164527039 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 599 0.5446048659853802 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 536 0.4873258900971015 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 494 0.44913990617158234 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 380 0.3454922355166018 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 362 0.3291268138342364 No Hit CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT 321 0.291850020002182 TruSeq Adapter, Index 1 (96% over 26bp) GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 287 0.26093755682438086 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 257 0.2336618540204386 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 257 0.2336618540204386 No Hit TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC 251 0.22820671345965016 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 245 0.22275157289886172 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 222 0.20184020074917264 No Hit CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG 211 0.19183910972106047 TruSeq Adapter, Index 1 (95% over 24bp) ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 197 0.1791104484125541 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 196 0.17820125831908934 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 196 0.17820125831908934 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 194 0.17638287813215986 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 191 0.17365530785176564 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 189 0.1718369276648362 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 176 0.1600174564497945 No Hit TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT 168 0.1527439357020766 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 167 0.15183474560861185 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 164 0.14910717532821763 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 161 0.1463796050478234 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 152 0.13819689420664072 No Hit GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 151 0.13728770411317598 No Hit GGTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 144 0.1309233734589228 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 144 0.1309233734589228 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 137 0.12455904280466959 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 134 0.12183147252427537 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 134 0.12183147252427537 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 131 0.11910390224388115 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 130 0.11819471215041642 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 126 0.11455795177655745 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 125 0.11364876168309271 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 124 0.11273957158962795 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 123 0.11183038149616323 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 121 0.11001200130923373 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATATCGTATGCCGT 120 0.109102811215769 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 115 0.1045568607484453 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 115 0.1045568607484453 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 114 0.10364767065498054 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 111 0.10092010037458632 No Hit TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG 110 0.10001091028112158 TruSeq Adapter, Index 1 (96% over 28bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 9.091900934647416E-4 0.0 12-13 0.0 0.0 0.0 0.0022729752336618538 0.0 14-15 0.0 0.0 0.0 0.0027275702803942247 0.0 16-17 0.0 0.0 0.0 0.0027275702803942247 0.0 18-19 0.0 0.0 0.0 0.0027275702803942247 0.0 20-21 0.0 0.0 0.0 0.006364330654253191 0.0 22-23 0.0 0.0 0.0 0.025911917663745135 0.0 24-25 0.0 0.0 0.0 0.07773575299123542 0.0 26-27 0.0 0.0 0.0 0.17547368803869512 0.0 28-29 0.0 0.0 0.0 0.42595555878823144 0.0 30-31 0.0 0.0 0.0 0.9810161108484563 0.0 32-33 0.0 0.0 0.0 1.6233589118812963 0.0 34-35 0.0 0.0 0.0 2.6007382623558932 0.0 36-37 0.0 0.0 0.0 3.907244426664727 0.0 38-39 0.0 0.0 0.0 5.236025748263447 0.0 40-41 0.0 0.0 0.0 6.584809251918391 0.0 42-43 0.0 0.0 0.0 7.519911263046877 0.0 44-45 0.0 0.0 0.0 8.32363530566971 0.0 46-47 0.0 0.0 0.0 9.184183729134087 0.0 48-49 0.0 0.0 0.0 9.909717423718952 0.0 50-51 0.0 0.0 0.0 10.540695348583482 0.0 52-53 0.0 0.0 0.0 10.994381205222389 0.0 54-55 0.0 0.0 0.0 11.478979525039094 0.0 56-57 0.0 0.0 0.0 11.907662654107721 0.0 58-59 0.0 0.0 0.0 12.107229879623233 0.0 60-61 0.0 0.0 0.0 12.25861003018511 0.0 62-63 0.0 0.0 0.0 12.36953122158781 0.0 64-65 0.0 0.0 0.0 12.472269702149326 0.0 66-67 0.0 0.0 0.0 12.552733025420956 0.0 68-69 0.0 0.0 0.0 12.664563406917118 0.0 70-71 0.0 0.0 0.0 12.848219805796996 0.0 72-73 0.0 0.0 0.0 13.051423791686366 0.0 74-75 0.0 0.0 0.0 13.336454885987562 0.0 76-77 0.0 0.0 0.0 13.65648979888715 0.0 78-79 0.0 0.0 0.0 14.13336000290941 0.0 80-81 0.0 0.0 0.0 14.62841400880096 0.0 82-83 0.0 0.0 0.0 15.057097137869587 0.0 84-85 0.0 0.0 0.0 15.425773720769538 0.0 86-87 0.0 0.0 0.0 15.72126050114558 9.091900934647416E-4 88-89 0.0 0.0 0.0 16.00674619049351 9.091900934647416E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGAGT 20 0.0021445688 70.60914 6 ATGGGGG 55 8.536954E-7 51.445683 5 AGTGTCG 15 0.009267173 48.803864 66-67 CTCTCGA 15 0.009868286 48.033875 88-89 TGGGGGA 45 0.0010022969 41.842453 6 TTGCGGT 25 0.001415176 39.024693 64-65 TTTGCGG 25 0.001415176 39.024693 64-65 TACTTGT 50 0.0016825097 37.65821 8 GGAAGCT 50 0.0016825097 37.65821 8 ATGGGCA 110 7.367271E-8 34.297123 5 ATACTAT 30 0.0034540063 32.535908 68-69 GATTTAA 30 0.004122407 31.38184 82-83 CCGGGCC 35 0.0074672266 27.789656 70-71 ATCCACC 35 0.00781609 27.53097 92-93 GGTATCA 3245 0.0 27.466763 1 GGGGCAG 70 0.008735792 26.89872 7 TGGGGCA 70 0.008735792 26.89872 6 GTTGTGT 140 1.8189894E-12 24.315948 70-71 GTATCAA 4230 0.0 21.405296 1 AGTACTC 375 0.0 21.37854 5 >>END_MODULE