##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939750_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 118880 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.106679004037684 31.0 31.0 33.0 30.0 34.0 2 31.22288862718708 31.0 31.0 34.0 28.0 34.0 3 31.14108344549125 31.0 31.0 34.0 28.0 34.0 4 35.01549461641992 35.0 35.0 37.0 32.0 37.0 5 34.8233765141319 35.0 35.0 37.0 32.0 37.0 6 34.96454407806191 36.0 35.0 37.0 32.0 37.0 7 34.816293741588154 35.0 35.0 37.0 32.0 37.0 8 34.89267328398385 35.0 35.0 37.0 32.0 37.0 9 36.20614064602961 38.0 35.0 39.0 32.0 39.0 10-11 36.17377607671602 38.0 35.0 39.0 31.0 39.0 12-13 36.05861793405114 38.0 35.0 39.0 30.0 39.0 14-15 36.581973418573355 38.0 35.5 40.0 30.0 41.0 16-17 36.57277086137282 38.0 35.5 40.0 30.5 41.0 18-19 36.85399141991925 38.5 35.5 40.0 31.0 41.0 20-21 36.94120541722746 39.0 35.0 40.0 31.0 41.0 22-23 36.671193640646024 38.0 35.0 40.0 31.0 41.0 24-25 36.316197005383586 38.0 35.0 40.0 30.0 41.0 26-27 35.73424041049798 38.0 34.5 40.0 29.5 41.0 28-29 35.284543236877525 37.0 34.0 40.0 29.0 41.0 30-31 34.47089922611036 36.5 33.0 39.0 27.0 40.5 32-33 33.412205585464335 35.0 33.0 39.0 24.5 40.0 34-35 32.645369279946166 35.0 33.0 38.0 21.0 40.0 36-37 31.747224091520863 35.0 31.5 38.0 13.5 40.0 38-39 30.93807200538358 35.0 31.0 38.0 4.5 40.0 40-41 30.442921433378196 35.0 30.5 38.0 2.0 40.0 42-43 29.52367934051144 35.0 28.5 38.0 2.0 40.0 44-45 28.471159993270525 35.0 24.0 38.0 2.0 40.0 46-47 27.26058209959623 35.0 18.5 38.0 2.0 40.0 48-49 26.205846231493943 34.0 4.0 38.0 2.0 40.0 50-51 25.387638795423957 33.0 2.0 38.0 2.0 40.0 52-53 24.803949360699868 33.0 2.0 37.5 2.0 40.0 54-55 24.128078734858683 33.0 2.0 37.0 2.0 40.0 56-57 23.230240578734858 32.5 2.0 36.5 2.0 40.0 58-59 22.42147543741588 31.0 2.0 36.0 2.0 40.0 60-61 21.469793068640648 30.5 2.0 35.5 2.0 39.0 62-63 20.704302658142666 28.5 2.0 35.0 2.0 39.0 64-65 19.87062584118439 26.5 2.0 35.0 2.0 39.0 66-67 18.636894347240915 22.5 2.0 35.0 2.0 38.0 68-69 17.10140477792732 9.5 2.0 34.0 2.0 37.0 70-71 16.492282133243606 7.5 2.0 33.0 2.0 36.0 72-73 16.62442378869448 4.5 2.0 33.0 2.0 35.5 74-75 16.551152422611036 2.0 2.0 33.0 2.0 35.0 76-77 16.16921685733513 2.0 2.0 32.5 2.0 35.0 78-79 16.323283983849258 2.0 2.0 33.0 2.0 35.0 80-81 16.117639636608345 2.0 2.0 33.0 2.0 35.0 82-83 15.788265477792732 2.0 2.0 33.0 2.0 35.0 84-85 15.531039703903096 2.0 2.0 33.0 2.0 35.0 86-87 15.306578061911171 2.0 2.0 33.0 2.0 35.0 88-89 14.983260430686407 2.0 2.0 33.0 2.0 35.0 90-91 14.623283983849259 2.0 2.0 32.0 2.0 35.0 92-93 14.28355905114401 2.0 2.0 32.0 2.0 35.0 94-95 13.790860531628534 2.0 2.0 31.5 2.0 35.0 96-97 13.435359185733514 2.0 2.0 31.0 2.0 34.0 98-99 13.001800134589502 2.0 2.0 31.0 2.0 34.0 100-101 12.26056527590848 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3081740677223479 1101 2 0.4319235899492391 1101 3 0.282347305037554 1101 4 0.062315635792742796 1101 5 0.26518066516715066 1101 6 0.019337016698791842 1101 7 0.0058926938558556685 1101 8 0.2453429653291792 1101 9 0.45587435834682566 1101 10-11 0.5275225730736182 1101 12-13 0.8962935548983353 1101 14-15 0.5784750340480187 1101 16-17 0.8648124848454444 1101 18-19 0.24170000559097815 1101 20-21 0.41306937802877286 1101 22-23 0.3782989521080111 1101 24-25 0.42823442868459693 1101 26-27 0.2587640617886606 1101 28-29 0.13072599309357713 1101 30-31 -0.039733946365458905 1101 32-33 0.5331093720240929 1101 34-35 0.5521417757091527 1101 36-37 0.07490349454781864 1101 38-39 0.2116969520601124 1101 40-41 0.4610676339361497 1101 42-43 -0.9616944422929308 1101 44-45 -0.7772663664480852 1101 46-47 -0.42083840265680905 1101 48-49 0.20478731103777292 1101 50-51 0.6957761711596149 1101 52-53 -0.05718711398700549 1101 54-55 -0.4189438710363014 1101 56-57 -0.48562091813851893 1101 58-59 0.004257425389084801 1101 60-61 0.12649094695409957 1101 62-63 0.02351794834301657 1101 64-65 0.06175756789145481 1101 66-67 0.5451572899525026 1101 68-69 -4.976060017315687 1101 70-71 -3.316951367104304 1101 72-73 0.38971066932312226 1101 74-75 1.2732347347016884 1101 76-77 0.9795684926332662 1101 78-79 0.7319030384130158 1101 80-81 0.7762439274214792 1101 82-83 0.9136164496523254 1101 84-85 0.7604239391353875 1101 86-87 1.2438857302643953 1101 88-89 1.1348296899966002 1101 90-91 0.9892291620149845 1101 92-93 0.8092389904639372 1101 94-95 0.664162172043536 1101 96-97 1.0701546904033723 1101 98-99 0.9132117000198878 1101 100-101 0.27841457445118145 1102 1 0.03438412411332692 1102 2 -0.3156680453891063 1102 3 0.24351976744356918 1102 4 0.08722165083033673 1102 5 0.23833621027993246 1102 6 0.32519932872886415 1102 7 0.15597493069795831 1102 8 0.23471091645699715 1102 9 0.17762012902351643 1102 10-11 0.40625260126910945 1102 12-13 0.21919064136450572 1102 14-15 0.6369806731457643 1102 16-17 0.5684661502589776 1102 18-19 0.13032782513985097 1102 20-21 0.1499702238934404 1102 22-23 0.2792329276719201 1102 24-25 0.42244847943647557 1102 26-27 0.8986548042698601 1102 28-29 0.6714919705371898 1102 30-31 1.04658536754431 1102 32-33 0.3602064114977779 1102 34-35 0.7630499054084012 1102 36-37 0.7239577708636098 1102 38-39 0.46160884115785805 1102 40-41 0.8874656602519373 1102 42-43 0.517158941165718 1102 44-45 -0.15855161080898839 1102 46-47 -0.17492087446131777 1102 48-49 -0.4100211946013239 1102 50-51 -1.3272207743291062 1102 52-53 -0.1872975463178328 1102 54-55 -0.08412244246487077 1102 56-57 -0.402502967010701 1102 58-59 -0.3169949242349759 1102 60-61 -0.15646253048950953 1102 62-63 0.38174750661369217 1102 64-65 0.8391894287937092 1102 66-67 0.733802731681827 1102 68-69 -2.2393647635562886 1102 70-71 0.1729158791362977 1102 72-73 0.24059765240583175 1102 74-75 0.08103548658138493 1102 76-77 0.22475176330995694 1102 78-79 0.4006647865333157 1102 80-81 0.2730131943387697 1102 82-83 -0.35711898267850195 1102 84-85 0.04502215342110283 1102 86-87 -0.2575240441716957 1102 88-89 -0.07951476489061804 1102 90-91 0.39398127667663907 1102 92-93 0.5470503156519069 1102 94-95 0.6223682340736119 1102 96-97 0.5532080268695392 1102 98-99 0.8649562958845483 1102 100-101 1.1484803639248664 1103 1 -0.09807055145919108 1103 2 0.1519488439238721 1103 3 -0.5129807096556682 1103 4 -0.08214857817729637 1103 5 0.14476512249367346 1103 6 0.35567404246932455 1103 7 0.1714210184842173 1103 8 0.5831722714188317 1103 9 0.33643453742046603 1103 10-11 0.29768270623094395 1103 12-13 0.3041739428912251 1103 14-15 0.003631436504541341 1103 16-17 0.3520420090376035 1103 18-19 0.028705687735275376 1103 20-21 0.3384840597713037 1103 22-23 0.0930151318703949 1103 24-25 0.10765243936013746 1103 26-27 -0.2338893274095355 1103 28-29 -0.018989899691817413 1103 30-31 0.29476642670461217 1103 32-33 0.7693488256199146 1103 34-35 -0.49443458886640457 1103 36-37 0.0339231811689551 1103 38-39 0.591051827799081 1103 40-41 0.3361178549084265 1103 42-43 0.4231703915473979 1103 44-45 0.037834362473454064 1103 46-47 0.7958866159966966 1103 48-49 0.6759461241009674 1103 50-51 0.17686438788463477 1103 52-53 0.5942506407050665 1103 54-55 -0.30334954170151107 1103 56-57 0.3293076245923494 1103 58-59 0.7886229192764738 1103 60-61 0.6578862290524761 1103 62-63 0.516200035239649 1103 64-65 0.9495782646715725 1103 66-67 0.9608721496207586 1103 68-69 -0.5064508799685008 1103 70-71 0.8355769001286646 1103 72-73 0.28028634515392525 1103 74-75 0.20269579192489573 1103 76-77 0.37921264117255404 1103 78-79 0.6955717521821718 1103 80-81 0.39711863327006824 1103 82-83 0.6184893188482175 1103 84-85 0.18463331754323953 1103 86-87 0.920433971095477 1103 88-89 0.5525820862544215 1103 90-91 0.3619261717148081 1103 92-93 0.25104005801831875 1103 94-95 -0.6636229395905104 1103 96-97 -0.46089621931366587 1103 98-99 -1.0774041525887341 1103 100-101 -1.8822925368384933 1104 1 0.39047906686141687 1104 2 0.5794297599544045 1104 3 0.700759748359598 1104 4 0.5056376813646892 1104 5 0.686749855318098 1104 6 0.6457503783471878 1104 7 0.5378332322246777 1104 8 0.25492799660966625 1104 9 0.6036101099395452 1104 10-11 0.6411941566126202 1104 12-13 0.3079907367843546 1104 14-15 0.38531082581751974 1104 16-17 0.5085305586559272 1104 18-19 0.5058049243764913 1104 20-21 0.7964993269468792 1104 22-23 0.4441601700383302 1104 24-25 0.8328432790547922 1104 26-27 0.8920648710637522 1104 28-29 0.6184146804608517 1104 30-31 0.9245374190710294 1104 32-33 1.17392897829167 1104 34-35 1.2918249531183292 1104 36-37 1.2896483720086458 1104 38-39 0.8620441942112933 1104 40-41 0.5842094579618582 1104 42-43 -0.7409517458571813 1104 44-45 -0.3972801413433409 1104 46-47 -0.28808284965215947 1104 48-49 -0.5759622728455973 1104 50-51 -0.609395154100099 1104 52-53 -0.7301768402109623 1104 54-55 -0.8949525951366262 1104 56-57 -0.8462648944916147 1104 58-59 -1.9174839509525334 1104 60-61 -1.861197740413175 1104 62-63 -1.815861033462646 1104 64-65 -2.1763759338017117 1104 66-67 -2.684166018318173 1104 68-69 -5.231641719663159 1104 70-71 -2.168239893764465 1104 72-73 -2.315133502174321 1104 74-75 -2.678983597388079 1104 76-77 -2.2429247634076006 1104 78-79 -2.067787026443783 1104 80-81 -1.9731103743635217 1104 82-83 -1.828075566647966 1104 84-85 -1.8000995068842407 1104 86-87 -1.6215507617289475 1104 88-89 -1.7031431045852745 1104 90-91 -1.9663181030943537 1104 92-93 -2.27186070534046 1104 94-95 -1.8201114892088306 1104 96-97 -2.3616595780922918 1104 98-99 -1.9629003357948402 1104 100-101 -2.2868726895102487 1105 1 -0.2455940735610902 1105 2 0.09585084995973148 1105 3 -0.19343517441689428 1105 4 -0.2621000545960186 1105 5 -0.19643849902239197 1105 6 -0.002986233286641493 1105 7 -0.4796234220152229 1105 8 -0.6576703819926166 1105 9 -0.2363212469454723 1105 10-11 -0.3656405673355394 1105 12-13 0.15881031190714623 1105 14-15 -0.29058715631160226 1105 16-17 -0.5254655357875393 1105 18-19 -0.7180248880384426 1105 20-21 -1.2306620435484135 1105 22-23 -0.6197193494598636 1105 24-25 -0.7267460457573165 1105 26-27 -0.8348420465053366 1105 28-29 -0.2973791534938215 1105 30-31 0.3528171989396611 1105 32-33 -0.24677638888982045 1105 34-35 -0.40292029807996954 1105 36-37 -0.7951495450278614 1105 38-39 -0.4635667886871033 1105 40-41 -0.8508458707558155 1105 42-43 -0.8738294309273051 1105 44-45 -1.9357439557702278 1105 46-47 -1.5388077446163564 1105 48-49 -1.2607661073920227 1105 50-51 -1.6992229545616624 1105 52-53 -2.7612437672480645 1105 54-55 -2.1841708920772724 1105 56-57 -3.0242931801889945 1105 58-59 -2.832922734312497 1105 60-61 -2.701217859946876 1105 62-63 -3.5146006038514273 1105 64-65 -2.790110474307067 1105 66-67 -2.060135010566235 1105 68-69 -1.8246058003779932 1105 70-71 -1.7280955065226173 1105 72-73 -1.8131511363538593 1105 74-75 -2.0018133506279163 1105 76-77 -2.08472013591485 1105 78-79 -2.1829713568775375 1105 80-81 -2.137173336668983 1105 82-83 -2.1857890408180367 1105 84-85 -2.0369182535556423 1105 86-87 -2.447931020915881 1105 88-89 -2.264773970316975 1105 90-91 -1.9476187717729747 1105 92-93 -1.6655526397744502 1105 94-95 -1.3271533260039075 1105 96-97 -1.636320800649841 1105 98-99 -1.358426698301498 1105 100-101 -1.3115487058425757 1106 1 0.14219662411332834 1106 2 0.06193612127756154 1106 3 -0.09033439922309583 1106 4 -0.041945015836333255 1106 5 0.09406537694660244 1106 6 0.1642618287288613 1106 7 0.21378743069795547 1106 8 0.12585674979033 1106 9 -0.11248403764314929 1106 10-11 0.19036718460244373 1106 12-13 0.04262814136450288 1106 14-15 -0.07760266018756568 1106 16-17 -0.17788801640768526 1106 18-19 -0.14050550819348473 1106 20-21 0.43174105722677325 1106 22-23 0.7352225110052544 1106 24-25 0.968021396103147 1106 26-27 0.47339438760319297 1106 28-29 0.4537836372038555 1106 30-31 0.8254916175443157 1106 32-33 0.6219251614977779 1106 34-35 -0.48043967792493447 1106 36-37 -0.061719312469723064 1106 38-39 -0.2860474088421405 1106 40-41 0.1119448269186023 1106 42-43 0.03382560783238375 1106 44-45 0.17530255585768018 1106 46-47 0.40033954220534795 1106 48-49 0.5105517220653439 1106 50-51 0.5636646423375602 1106 52-53 0.15280662034883363 1106 54-55 0.2221275575351278 1106 56-57 -0.07594046701069956 1106 58-59 1.0533175757650213 1106 60-61 1.2700999695104898 1106 62-63 0.6861745899470257 1106 64-65 -0.202998071206288 1106 66-67 -0.23416601831817374 1106 68-69 0.20204148644371145 1106 70-71 0.3216137958029641 1106 72-73 0.3778372357391646 1106 74-75 0.6977021532480521 1106 76-77 0.4195434299766241 1106 78-79 0.3199356198666514 1106 80-81 0.3547840276720997 1106 82-83 0.24444351732149805 1106 84-85 -2.9034657889681625E-4 1106 86-87 0.013309289161636428 1106 88-89 -0.7071189315572841 1106 90-91 -1.414091639990028 1106 92-93 -1.3787309343480931 1106 94-95 -0.7784130159263878 1106 96-97 -0.2960107231304594 1106 98-99 -0.05561662078211782 1106 100-101 -0.02026963607513288 1107 1 -0.0977287490210017 1107 2 0.00907542476014811 1107 3 0.2643919689361063 1107 4 0.15905000903929079 1107 5 0.0180703520709784 1107 6 0.1235901869378182 1107 7 0.47722026651885585 1107 8 0.08767267018833991 1107 9 -0.08648901276752952 1107 10-11 0.20889952291090452 1107 12-13 0.6017699324092831 1107 14-15 0.6113276880711354 1107 16-17 0.7460547696619599 1107 18-19 0.7450666311099994 1107 20-21 0.6491539925501613 1107 22-23 0.2882697746868459 1107 24-25 0.3965039831678183 1107 26-27 0.15069538262806503 1107 28-29 0.6572662242685325 1107 30-31 0.5691483339622181 1107 32-33 0.5444997883634457 1107 34-35 0.37155037182630934 1107 36-37 -0.23715463585280716 1107 38-39 0.6587287105608404 1107 40-41 0.04826323487880302 1107 42-43 0.5871216277328806 1107 44-45 0.6619940981462342 1107 46-47 -0.3178196617747524 1107 48-49 0.019258189727036523 1107 50-51 0.8524084234320881 1107 52-53 1.1728936850254499 1107 54-55 1.4424633784306522 1107 56-57 1.8753655031385499 1107 58-59 2.266377277257561 1107 60-61 2.0775626560776566 1107 62-63 1.611050211837572 1107 64-65 1.8274123765549035 1107 66-67 2.1926996533236185 1107 68-69 2.8615564118168457 1107 70-71 3.5730441440616687 1107 72-73 3.3853621113610544 1107 74-75 2.7965205612082507 1107 76-77 3.078996166295031 1107 78-79 2.5145873611601814 1107 80-81 2.64253278389101 1107 82-83 2.860115159112544 1107 84-85 2.2135156235703537 1107 86-87 2.01206550806711 1107 88-89 2.313092511228785 1107 90-91 1.7380227878706673 1107 92-93 2.1063436925175782 1107 94-95 3.0109526557154034 1107 96-97 2.264437038063569 1107 98-99 2.9851172100636525 1107 100-101 2.778610960939792 1108 1 -0.42126491434821034 1108 2 -0.4220382376967997 1108 3 -0.6390523479410462 1108 4 -0.0752783491696647 1108 5 0.198552556433782 1108 6 0.05849259795963491 1108 7 0.07917204608257578 1108 8 -0.15939966046607879 1108 9 0.2561057059465952 1108 10-11 -0.2756584564231943 1108 12-13 -0.2660257047893424 1108 14-15 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-0.5437914029737954 2316 72-73 -0.559968574658388 2316 74-75 -0.9596755225929279 2316 76-77 -1.6053801174239872 2316 78-79 -1.526012392365768 2316 80-81 -1.1410493056612303 2316 82-83 -0.9252812533207031 2316 84-85 -1.5207031906155937 2316 86-87 -1.9622258790340812 2316 88-89 -1.4458421731474989 2316 90-91 -1.603617633873819 2316 92-93 -1.5301070811370838 2316 94-95 -1.7237111810640027 2316 96-97 -1.4235336589102783 2316 98-99 -0.7450279357668279 2316 100-101 -0.10352651680907776 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 15.0 5 42.0 6 104.0 7 243.0 8 486.0 9 888.0 10 1448.0 11 2267.0 12 3122.0 13 3678.0 14 4239.0 15 5043.0 16 5436.0 17 6068.0 18 5164.0 19 4142.0 20 4000.0 21 3757.0 22 3602.0 23 3240.0 24 3095.0 25 2847.0 26 2301.0 27 2227.0 28 2417.0 29 2765.0 30 3157.0 31 3531.0 32 4012.0 33 4693.0 34 6052.0 35 7860.0 36 7112.0 37 6659.0 38 2991.0 39 175.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.54180129240711 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39.790655275933354 15.042336307330856 44-45 11.410263427120743 32.95596636957263 39.44670621371404 16.187063989592588 46-47 13.607163304482539 31.422389115279696 37.54841208365608 17.422035496581685 48-49 13.151890207964975 30.6504167719121 36.66329881283153 19.534394207291403 50-51 11.295265679332497 30.46998004563404 38.6403246583762 19.594429616657262 52-53 11.9280921955367 30.402976656480714 38.85226995311093 18.816661194871664 54-55 11.615007470067125 29.379037518675165 36.58060307640511 22.425351934852596 56-57 14.339568660119994 29.626637719940422 34.01450702210554 22.019286597834046 58-59 12.674995793370353 30.85058051489147 35.96415951539627 20.510264176341913 60-61 13.723378748427859 32.95587907442383 32.349736889704 20.971005287444317 62-63 11.737490063133277 34.31363064718971 31.930885674508204 22.01799361516881 64-65 12.125380140573144 33.95207389554179 31.67396452441943 22.248581439465635 66-67 13.066723965772212 32.143955576340524 31.142906497128724 23.64641396075854 68-69 14.009167916517038 30.378757472696932 31.50341156340438 24.10866304738165 70-71 14.934129662559714 29.59793540102977 31.00446456855879 24.463470367851727 72-73 14.590541856142819 28.04233027221349 31.63981338267089 25.7273144889728 74-75 13.998291813748795 27.926320794012092 31.335120056883444 26.740267335355668 76-77 14.009466708740929 27.523719364678655 31.45892500262964 27.007888923950773 78-79 14.266005459585191 27.098974960777632 31.921328134529563 26.713691445107614 80-81 14.44554268049042 27.170406510904083 31.16527517822969 27.21877563037581 82-83 14.332099596231492 27.302742261103635 30.75033647375505 27.614821668909823 84-85 14.738874238199184 27.27720086305881 30.143969313464474 27.839955585277533 86-87 15.26426029828649 27.048511511705183 29.66587875066244 28.02134943934589 88-89 15.24814939434724 27.03861036339166 28.91234858681023 28.800891655450876 90-91 15.580613819992681 26.233086722998696 29.27695083635813 28.909348620650494 92-93 15.59026039848413 26.24594638928964 29.271380562016564 28.892412650209675 94-95 15.639116103039921 25.323420575147658 29.70122800887433 29.336235312938086 96-97 15.03411777573978 25.603055875745667 29.98073250149344 29.382093847021114 98-99 15.85102668411111 25.7316780742679 29.216219968280278 29.20107527334071 100-101 14.838386727688787 25.84718670076726 29.576659038901603 29.737767532642344 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2311.0 1 1413.0 2 570.0 3 621.0 4 493.0 5 398.5 6 596.0 7 991.0 8 1294.0 9 1306.5 10 1000.5 11 672.5 12 581.5 13 542.0 14 549.5 15 556.0 16 567.5 17 631.0 18 694.0 19 779.0 20 867.5 21 966.0 22 1083.5 23 1258.0 24 1432.0 25 1604.5 26 1823.0 27 2090.5 28 2374.0 29 2599.0 30 2848.0 31 3108.5 32 3280.5 33 3388.5 34 3475.5 35 3432.5 36 3308.5 37 3161.5 38 2941.5 39 2760.0 40 2613.0 41 2487.0 42 2474.5 43 2527.5 44 2498.5 45 2461.5 46 2715.0 47 3540.0 48 4965.5 49 5791.5 50 6053.5 51 5495.5 52 3410.0 53 1650.0 54 969.0 55 767.5 56 676.0 57 611.0 58 587.5 59 541.5 60 484.5 61 440.5 62 374.5 63 300.5 64 258.0 65 233.5 66 206.0 67 157.5 68 108.0 69 78.0 70 56.5 71 39.0 72 29.0 73 21.5 74 13.0 75 8.5 76 6.0 77 4.5 78 3.5 79 2.0 80 2.0 81 1.5 82 0.5 83 0.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026917900403768503 2 0.023553162853297442 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 8.411843876177657E-4 28-29 0.0 30-31 0.0 32-33 0.0012617765814266488 34-35 0.0025235531628532977 36-37 0.10178331090174968 38-39 0.0937920592193809 40-41 0.10304508748317631 42-43 0.10809219380888291 44-45 0.09968034993270525 46-47 0.0908479138627187 48-49 0.09253028263795424 50-51 0.09168909825033647 52-53 0.07486541049798116 54-55 0.060985868102288024 56-57 0.0344885598923284 58-59 0.016823687752355317 60-61 0.011355989232839838 62-63 0.003785329744279946 64-65 0.007991251682368776 66-67 0.1720222072678331 68-69 0.4479306864064603 70-71 0.4235363391655451 72-73 0.20314602960969044 74-75 0.03406796769851952 76-77 0.03575033647375504 78-79 0.0054676985195154775 80-81 0.0021029609690444147 82-83 0.0 84-85 4.2059219380888286E-4 86-87 8.411843876177657E-4 88-89 0.0 90-91 0.0012617765814266488 92-93 0.003785329744279946 94-95 0.09295087483176312 96-97 0.022711978465679676 98-99 0.022291386271870797 100-101 0.013458950201884251 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 118880.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.80299461641992 #Duplication Level Percentage of deduplicated Percentage of total 1 82.02715418739078 35.93034993270525 2 8.44007451078294 7.394010767160161 3 3.3856317093311312 4.449024226110363 4 1.7897950953469168 3.135935397039031 5 1.0389261229427917 2.2754037685060564 6 0.6682925892497071 1.756393001345895 7 0.5146621089624182 1.5780619111709286 8 0.3533501046607647 1.2382234185733512 9 0.2592514354848002 1.0220390309555853 >10 1.3039387014383654 9.942799461641991 >50 0.09985981218673784 3.074528936742934 >100 0.09217828817237339 7.291386271870794 >500 0.013442667025137788 4.227792732166891 >1k 0.013442667025137788 16.684051144010766 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4016 3.3781965006729475 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3779 3.178835800807537 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 3461 2.9113391655450878 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3336 2.806191117092867 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2608 2.1938088829071334 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 1583 1.3315948855989235 TruSeq Adapter, Index 1 (95% over 21bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1051 0.8840847913862718 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 980 0.8243606998654105 No Hit ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT 878 0.7385598923283985 RNA PCR Primer, Index 1 (95% over 23bp) GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 785 0.6603297442799462 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 606 0.5097577388963661 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 603 0.5072341857335128 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 592 0.49798115746971733 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 582 0.4895693135935397 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 434 0.36507402422611035 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 387 0.32553835800807535 No Hit CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT 373 0.3137617765814267 TruSeq Adapter, Index 1 (96% over 26bp) GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 295 0.24814939434724093 No Hit CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG 268 0.22543741588156124 TruSeq Adapter, Index 1 (95% over 24bp) GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 266 0.2237550471063257 No Hit TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC 264 0.2220726783310902 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 262 0.22039030955585465 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 247 0.20777254374158816 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 241 0.20272543741588156 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 234 0.1968371467025572 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 217 0.18253701211305517 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 215 0.18085464333781964 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 205 0.172442799461642 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 204 0.17160161507402422 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 201 0.16907806191117092 No Hit TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT 191 0.16066621803499326 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 186 0.15646029609690443 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 184 0.1547779273216689 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 183 0.15393674293405116 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 180 0.15141318977119786 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 162 0.13627187079407807 No Hit GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 155 0.1303835800807537 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 154 0.12954239569313594 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 149 0.1253364737550471 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 149 0.1253364737550471 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 143 0.12028936742934052 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 143 0.12028936742934052 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 142 0.11944818304172275 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 138 0.11608344549125169 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 138 0.11608344549125169 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 137 0.1152422611036339 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 137 0.1152422611036339 No Hit GGTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 130 0.10935397039030954 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 126 0.1059892328398385 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATATCGTATGCCGT 124 0.10430686406460296 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.411843876177658E-4 0.0 10-11 0.0 0.0 0.0 0.0016823687752355316 0.0 12-13 0.0 0.0 0.0 0.0025235531628532972 0.0 14-15 0.0 0.0 0.0 0.0058882907133243605 0.0 16-17 0.0 0.0 0.0 0.007570659488559893 0.0 18-19 0.0 0.0 0.0 0.008832436069986542 0.0 20-21 0.0 0.0 0.0 0.012197173620457604 0.0 22-23 0.0 0.0 0.0 0.026497308209959623 0.0 24-25 0.0 0.0 0.0 0.07444481830417227 0.0 26-27 0.0 0.0 0.0 0.17959286675639302 0.0 28-29 0.0 0.0 0.0 0.4559219380888291 0.0 30-31 0.0 0.0 0.0 0.973670928667564 0.0 32-33 0.0 0.0 0.0 1.6352624495289367 0.0 34-35 0.0 0.0 0.0 2.6749663526244953 0.0 36-37 0.0 0.0 0.0 3.996467025572005 0.0 38-39 0.0 0.0 0.0 5.356241588156124 0.0 40-41 0.0 0.0 0.0 6.686995289367429 0.0 42-43 0.0 0.0 0.0 7.672022207267833 0.0 44-45 0.0 0.0 0.0 8.462735531628534 0.0 46-47 0.0 0.0 0.0 9.303499327052489 0.0 48-49 0.0 0.0 0.0 10.174966352624494 0.0 50-51 0.0 0.0 0.0 10.935397039030956 0.0 52-53 0.0 0.0 0.0 11.521281965006729 0.0 54-55 0.0 0.0 0.0 12.137028936742935 0.0 56-57 0.0 0.0 0.0 12.778432032301481 0.0 58-59 0.0 0.0 0.0 13.257907133243608 0.0 60-61 0.0 0.0 0.0 13.710884925975773 0.0 62-63 0.0 0.0 0.0 14.131477119784657 0.0 64-65 0.0 0.0 0.0 14.524730820995963 0.0 66-67 0.0 0.0 0.0 14.926396366083445 0.0 68-69 0.0 0.0 0.0 15.41428331090175 0.0 70-71 0.0 0.0 0.0 15.932452893674292 0.0 72-73 0.0 0.0 0.0 16.399730820995963 0.0 74-75 0.0 0.0 0.0 16.88341184387618 0.0 76-77 0.0 0.0 0.0 17.36541049798116 0.0 78-79 0.0 0.0 0.0 17.891571332436072 0.0 80-81 0.0 0.0 0.0 18.36978465679677 0.0 82-83 0.0 0.0 0.0 18.826547779273216 0.0 84-85 0.0 0.0 0.0 19.30770524899058 0.0 86-87 0.0 0.0 0.0 19.738391655450876 0.0 88-89 0.0 0.0 8.411843876177658E-4 20.189687079407804 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAAGA 40 9.052201E-8 71.18058 9 GAGATGT 40 7.547571E-6 59.317154 1 AGATGTG 40 7.547571E-6 59.317154 2 CGGGACC 35 2.7911633E-4 54.232826 9 TGTATAA 55 8.1830694E-7 51.767696 7 GTATAAG 55 8.1830694E-7 51.767696 8 CATGGCG 40 5.394446E-4 47.453724 4 ATGGCGG 40 5.394446E-4 47.453724 5 GCGGGAC 40 5.394446E-4 47.453724 8 TGTGTAT 55 4.9648585E-5 43.139748 5 GTGTATA 60 8.293429E-5 39.54477 6 GGGGCAG 100 3.0175215E-8 37.962982 7 ATGGGGC 100 3.0175215E-8 37.962982 5 GTACCCA 100 3.0175215E-8 37.962982 1 TGGCGGG 50 0.0016177194 37.962982 6 TGGGGCA 100 3.0175215E-8 37.962982 6 ATGGGGG 65 1.3286286E-4 36.502865 5 GATGTGT 65 1.3286286E-4 36.502865 3 ATGTGTA 65 1.3286286E-4 36.502865 4 GACACAT 40 1.0812177E-5 35.59029 20-21 >>END_MODULE