FastQCFastQC Report
Fri 27 May 2016
SRR939749_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939749_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2946453
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163460.554768733796195No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113600.38554831860545546No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74730.2536269881107895No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89050.046.4500581
GTATCAA165100.041.717981
TATCAAC210000.033.6566352
ATCAACG212300.033.2065853
TCAACGC214950.032.8854834
CAACGCA217400.032.4499635
AACGCAG222550.031.7416766
TACATGG184850.028.7410262
GTACATG186250.027.9646761
ACGCAGA252700.027.8231127
CGCAGAG252900.027.6505688
ACATGGG194850.026.5875073
GCAGAGT255750.025.8959929
CATGGGG91100.024.423614
AGTACTT146250.023.01368112-13
GAGTACT143700.022.9433912-13
CATTCCG6000.022.139599
AGAGTAC239850.021.50821310-11
GTACTTT159200.020.75692414-15
TAGCCCT31350.020.5805384
Produced by FastQC (version 0.11.5)