FastQCFastQC Report
Fri 27 May 2016
SRR939749_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939749_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2946453
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182290.6186760827340534No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137080.4652373548805971No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96420.327240923238891No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95950.046.695821
GTATCAA170750.040.1056251
TATCAAC219900.031.047892
ATCAACG222200.030.6395513
TCAACGC224700.030.277164
CAACGCA228550.029.7465025
AACGCAG234850.029.0101176
GTACATG191700.026.029331
TACATGG191050.025.7163562
ACGCAGA268600.025.25977
CGCAGAG268100.025.2013658
ACATGGG194700.024.215493
GCAGAGT282400.023.3913389
GTATAGC16200.023.290261
CATGGGG89950.022.8526844
TAGGACG13300.022.3326784
GAGTACT161600.021.75049812-13
AGTACTT163150.021.74601412-13
GTAGGAC15350.021.1933673
TAGCCCT33350.021.064074
Produced by FastQC (version 0.11.5)