FastQCFastQC Report
Fri 27 May 2016
SRR939748_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939748_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3102882
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173510.5591898112786758No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119210.3841912132011465No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81910.2639803898440224No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31450.10135738323274943No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98450.046.108811
GTATCAA179250.042.4086531
TATCAAC226950.033.329992
ATCAACG228400.033.1601223
TCAACGC231850.032.6677444
CAACGCA235250.032.156135
AACGCAG240950.031.4945816
TACATGG193350.028.1250292
GTACATG198100.027.8776341
ACGCAGA270000.027.7052297
CGCAGAG270550.027.5097948
ACATGGG203950.025.9717373
GCAGAGT277750.025.4415919
CATGGGG92600.023.6233964
GTATAGC18700.023.1429391
GAGTACT156100.022.93504712-13
AGTACTT157400.022.55134212-13
TAGCCCT33650.021.6694054
AGAGTAC255950.021.30326510-11
GTACTTT170950.020.97516414-15
Produced by FastQC (version 0.11.5)