FastQCFastQC Report
Fri 27 May 2016
SRR939748_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939748_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3102882
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188660.6080153869853897No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141840.4571234097848388No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102080.32898447314464424No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98350.044.9705921
GTATCAA178350.040.9232671
ATCAACG227750.031.9089453
TATCAAC229700.031.6875322
TCAACGC230200.031.6105544
CAACGCA234350.031.0710185
AACGCAG239950.030.3854146
TACATGG197150.027.5089172
GTACATG200050.027.4461781
ACGCAGA277050.026.145267
CGCAGAG278600.025.9146658
ACATGGG203800.025.5570223
GCAGAGT291150.024.1459059
CGGGAGT10650.023.1614514
GTCGGGA10950.022.9660242
CATGGGG94650.022.352514
AGTACTT163450.022.10024312-13
GTATAGC17250.022.0083731
GAGTACT161600.021.73680512-13
AGAGTAC265450.020.90812910-11
Produced by FastQC (version 0.11.5)