##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939747_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 255703 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.17433506841922 31.0 31.0 33.0 30.0 34.0 2 31.256899606183737 31.0 31.0 34.0 28.0 34.0 3 31.292597271052745 31.0 31.0 34.0 28.0 34.0 4 35.00917079580607 35.0 35.0 37.0 32.0 37.0 5 34.806631130647666 35.0 35.0 37.0 32.0 37.0 6 34.96878800796236 35.0 35.0 37.0 32.0 37.0 7 34.919277442970944 35.0 35.0 37.0 32.0 37.0 8 34.96018427628929 36.0 35.0 37.0 32.0 37.0 9 36.318795633997254 38.0 35.0 39.0 32.0 39.0 10-11 36.16758309444942 38.0 35.0 39.0 31.5 39.0 12-13 36.211333070007 38.0 35.0 39.0 31.0 39.0 14-15 36.70717199250693 38.0 36.0 40.0 31.0 41.0 16-17 36.78782219997419 38.0 35.5 40.0 31.0 41.0 18-19 37.00708634626891 38.5 36.0 40.0 31.0 41.0 20-21 36.94486963391122 39.0 35.5 40.0 31.0 41.0 22-23 36.7771418403382 38.5 35.0 40.0 31.0 41.0 24-25 36.34636472782877 38.0 34.5 40.0 30.5 41.0 26-27 35.837518527354 38.0 34.5 40.0 28.5 41.0 28-29 35.73634255366578 38.0 34.0 40.0 29.5 41.0 30-31 35.42758199942902 37.5 34.0 40.0 29.0 41.0 32-33 34.99035404355835 37.0 33.5 40.0 27.0 41.0 34-35 34.6140111770296 37.0 33.0 40.0 26.0 41.0 36-37 34.350912973254125 37.0 33.0 40.0 24.5 41.0 38-39 33.915855504237335 37.0 33.0 40.0 24.0 41.0 40-41 33.902547095653944 37.0 33.0 40.0 23.5 41.0 42-43 33.37708004990164 37.0 33.0 40.0 20.0 41.0 44-45 32.878278706155186 37.0 33.0 40.0 15.0 41.0 46-47 32.278315076475444 37.0 32.0 40.0 5.0 41.0 48-49 31.612081203583845 36.0 31.0 40.0 2.0 41.0 50-51 31.04609253704493 36.0 30.0 39.0 2.0 41.0 52-53 30.86804222085779 36.0 30.0 40.0 2.0 41.0 54-55 30.514567682037363 36.0 28.5 40.0 2.0 41.0 56-57 28.910366714508626 35.0 25.0 39.0 2.0 40.5 58-59 25.588956719318897 32.5 10.0 38.0 2.0 40.0 60-61 25.147561819767464 31.5 10.5 37.5 2.0 40.0 62-63 26.63727840502458 33.5 19.0 38.0 2.0 40.0 64-65 26.152722103377748 33.5 7.5 37.5 2.0 39.5 66-67 21.109416002158753 22.5 2.0 34.0 2.0 37.5 68-69 14.7520873826275 11.0 2.0 27.0 2.0 34.5 70-71 13.449400280794515 10.5 2.0 23.5 2.0 31.5 72-73 15.16088782689292 12.0 2.0 27.5 2.0 33.0 74-75 15.856276617794865 12.0 2.0 29.0 2.0 34.0 76-77 16.64223728309797 16.0 2.0 29.5 2.0 33.5 78-79 19.007051149184797 23.5 2.0 32.5 2.0 34.5 80-81 20.794341482110106 28.0 2.0 34.0 2.0 35.0 82-83 21.416015846509428 29.5 2.0 34.0 2.0 35.0 84-85 21.327843240008917 30.0 2.0 34.0 2.0 35.0 86-87 21.32089377128935 30.0 2.0 34.0 2.0 35.0 88-89 20.88987614537178 29.5 2.0 34.0 2.0 35.0 90-91 19.592073616656826 27.5 2.0 34.0 2.0 35.0 92-93 18.02651904748869 24.0 2.0 33.5 2.0 35.0 94-95 14.594148289226172 2.0 2.0 31.0 2.0 34.0 96-97 13.555083436643294 2.0 2.0 30.5 2.0 34.0 98-99 12.721594975420704 2.0 2.0 30.0 2.0 34.0 100-101 12.120610630301561 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.06074159383047828 1101 2 0.07070384602056023 1101 3 -0.2280849690800899 1101 4 -0.14235306725566943 1101 5 0.5460430071073574 1101 6 0.5537947667181982 1101 7 0.011930391431427267 1101 8 0.38899949512865106 1101 9 0.9249539357046288 1101 10-11 0.21431061973580512 1101 12-13 0.477978600764807 1101 14-15 0.055960801672569005 1101 16-17 -0.17145123834630738 1101 18-19 0.6301711917670971 1101 20-21 0.2649481112779313 1101 22-23 0.3349760435708333 1101 24-25 0.05847062435199746 1101 26-27 -0.33990760373148277 1101 28-29 0.15827705609630982 1101 30-31 0.11520256205929513 1101 32-33 0.1603777847535497 1101 34-35 0.5116767556393214 1101 36-37 -0.12008589324349828 1101 38-39 0.3481002820863921 1101 40-41 0.23288731616571567 1101 42-43 0.18343395524366457 1101 44-45 -1.384019391173453 1101 46-47 -1.1519625381786938 1101 48-49 -1.75559942579984 1101 50-51 -0.9310728938304926 1101 52-53 -1.2012091669796234 1101 54-55 -0.7165760455811387 1101 56-57 -0.24738897725204367 1101 58-59 -8.626509666457263 1101 60-61 -9.207020319089935 1101 62-63 -4.037953847324971 1101 64-65 -1.4280573929443428 1101 66-67 -1.2101799997289966 1101 68-69 -4.467112415008675 1101 70-71 -3.767811780145612 1101 72-73 -5.1852100461888195 1101 74-75 -6.532400073688786 1101 76-77 -6.522417838065213 1101 78-79 -9.369667898740346 1101 80-81 -6.244624536992191 1101 82-83 -3.5597848680957327 1101 84-85 -2.867044777036181 1101 86-87 -2.7790169007184353 1101 88-89 -3.760462327739294 1101 90-91 -7.278827066782124 1101 92-93 -12.383395173608466 1101 94-95 -9.835270345391967 1101 96-97 -9.877591859067508 1101 98-99 -9.644210378721262 1101 100-101 -9.513347313853068 1102 1 0.6395088673553744 1102 2 0.508738502743526 1102 3 0.4634263272568262 1102 4 0.2872586897085796 1102 5 0.42908753441597725 1102 6 0.6123163723334599 1102 7 0.617326311589359 1102 8 0.39233355961648186 1102 9 0.5968556685407762 1102 10-11 0.7640583714357376 1102 12-13 0.8296566003261105 1102 14-15 1.3892356426464687 1102 16-17 0.9520626901467821 1102 18-19 0.8303686034801885 1102 20-21 0.7941211755464082 1102 22-23 0.7435963550426479 1102 24-25 0.912714976183544 1102 26-27 0.9479844778653614 1102 28-29 0.49696975186292747 1102 30-31 0.7524475719298778 1102 32-33 0.638640561678308 1102 34-35 0.527996123921838 1102 36-37 0.20042299028359167 1102 38-39 0.5324389528475209 1102 40-41 0.30263945479260457 1102 42-43 0.7405736788672712 1102 44-45 0.5763008544944555 1102 46-47 0.47155139031439575 1102 48-49 0.3988862070143746 1102 50-51 0.7948977137588926 1102 52-53 1.0348294379776029 1102 54-55 1.1018929169106357 1102 56-57 1.558577243410383 1102 58-59 -7.444978628949038 1102 60-61 -7.818755348701693 1102 62-63 -2.1712944922032342 1102 64-65 0.16121657064416084 1102 66-67 0.027218552585107858 1102 68-69 -2.5611461943462484 1102 70-71 -3.3058025675990006 1102 72-73 -5.43381719687976 1102 74-75 -6.1552559631382255 1102 76-77 -7.267910270616211 1102 78-79 -9.423188407623874 1102 80-81 -6.453135175290063 1102 82-83 -3.4037199415767603 1102 84-85 -2.3765863431691017 1102 86-87 -2.392791851365505 1102 88-89 -2.241401130108237 1102 90-91 -6.680472164391249 1102 92-93 -11.52774700515047 1102 94-95 -10.136235469322653 1102 96-97 -9.988888195983499 1102 98-99 -9.310014283086254 1102 100-101 -8.717602633002004 1103 1 0.3462058917724278 1103 2 0.2988774679629671 1103 3 0.16187965221560674 1103 4 0.23608494392856727 1103 5 0.31003281195386023 1103 6 0.29305752017047126 1103 7 0.5926737246379616 1103 8 0.5787510833661855 1103 9 0.37289398211540004 1103 10-11 0.3182783604441397 1103 12-13 0.3545525726900891 1103 14-15 0.6839518238508333 1103 16-17 0.2931320157336543 1103 18-19 0.7579339642401877 1103 20-21 0.34962875189877707 1103 22-23 0.28479915004932366 1103 24-25 0.4188388672096792 1103 26-27 0.43652181004718926 1103 28-29 0.7090055530720036 1103 30-31 0.5758675295337099 1103 32-33 0.48219084824501124 1103 34-35 0.11952474133567392 1103 36-37 -0.29283287844531003 1103 38-39 0.21409868433845958 1103 40-41 0.05654539810736736 1103 42-43 0.40658608367216686 1103 44-45 0.6017150022529592 1103 46-47 0.8052406421005287 1103 48-49 0.4036047435614414 1103 50-51 0.1726475763639037 1103 52-53 0.7947964631797682 1103 54-55 1.1262550115954113 1103 56-57 0.6440212255097819 1103 58-59 -6.166757697803554 1103 60-61 -7.009679428155252 1103 62-63 -1.7362077370803632 1103 64-65 0.04713947242636962 1103 66-67 -0.9385818713765808 1103 68-69 -3.131052765752388 1103 70-71 -3.6865358642936705 1103 72-73 -5.929836667712767 1103 74-75 -6.150443212883063 1103 76-77 -6.681736132279081 1103 78-79 -9.283712863932266 1103 80-81 -6.4734068248150685 1103 82-83 -3.188441618580761 1103 84-85 -2.0736107602217864 1103 86-87 -2.0919282257079743 1103 88-89 -2.2022396321102775 1103 90-91 -6.295546357686373 1103 92-93 -11.349424009154554 1103 94-95 -9.403307778343617 1103 96-97 -9.347748210115554 1103 98-99 -9.198590870979793 1103 100-101 -9.172018900568935 1104 1 0.06646461527220993 1104 2 0.17705012801656395 1104 3 0.32230505875104143 1104 4 0.19977385838425477 1104 5 0.018505866266146143 1104 6 0.2719555601014463 1104 7 0.14171754207232823 1104 8 0.4209072996051546 1104 9 0.9019442536067075 1104 10-11 0.5863462122253154 1104 12-13 0.3850970986676714 1104 14-15 0.4386976602825783 1104 16-17 0.4055939578435712 1104 18-19 0.31181832437656 1104 20-21 0.3064988148959742 1104 22-23 0.6116410116238313 1104 24-25 0.7243402492197006 1104 26-27 0.5748350562975233 1104 28-29 0.7611150475509731 1104 30-31 0.3865952946060318 1104 32-33 0.050856401820695396 1104 34-35 0.6159259030659214 1104 36-37 0.5440197866592911 1104 38-39 0.5236128060956489 1104 40-41 0.48078038190206485 1104 42-43 0.890521094124928 1104 44-45 0.3729677908264719 1104 46-47 0.4745123158058604 1104 48-49 0.510187881636174 1104 50-51 0.18509268180354965 1104 52-53 -0.045374429023446794 1104 54-55 0.30308214108343634 1104 56-57 1.4339837639184339 1104 58-59 -6.378107016619936 1104 60-61 -6.962263155513437 1104 62-63 -1.1250927285918735 1104 64-65 0.5281771725372124 1104 66-67 -0.635486730159009 1104 68-69 -3.1545609666138645 1104 70-71 -3.3779407441219362 1104 72-73 -6.060538335946179 1104 74-75 -7.407630366503649 1104 76-77 -7.835518069337997 1104 78-79 -9.977229342823291 1104 80-81 -6.689491457144282 1104 82-83 -3.9834060511148195 1104 84-85 -3.3912696212210385 1104 86-87 -3.283269968022733 1104 88-89 -3.3156346063642026 1104 90-91 -7.405987899364149 1104 92-93 -12.021080877814494 1104 94-95 -10.588553242965563 1104 96-97 -10.125034138868378 1104 98-99 -9.559541020405241 1104 100-101 -8.866959481153447 1105 1 0.1791545399840082 1105 2 0.13711859937826532 1105 3 0.02833265937516316 1105 4 0.021445347333397535 1105 5 -0.38191526290538036 1105 6 -0.1287533911657306 1105 7 0.15441201134353832 1105 8 0.009897347020057623 1105 9 -0.1037970397748964 1105 10-11 0.01849594030917956 1105 12-13 0.2811781736074508 1105 14-15 -0.22742113450026125 1105 16-17 -0.03079735901825842 1105 18-19 0.035539409617349804 1105 20-21 -0.014347079094598314 1105 22-23 -0.19568481813698213 1105 24-25 0.035839499367398275 1105 26-27 -0.05375325206598092 1105 28-29 -0.47122555202788874 1105 30-31 -0.44383961972901886 1105 32-33 -0.46144420563540933 1105 34-35 -0.22356783301636796 1105 36-37 -0.2719937258306757 1105 38-39 -0.18627767002269735 1105 40-41 -0.09809802083679386 1105 42-43 -0.002030373010327935 1105 44-45 -0.6877323742925157 1105 46-47 -0.015292722595667385 1105 48-49 -0.5911027732348408 1105 50-51 -0.9780191295063432 1105 52-53 -0.6759699177908125 1105 54-55 -0.721299420124204 1105 56-57 -0.19302909718468442 1105 58-59 -6.759236491117385 1105 60-61 -7.864555128306677 1105 62-63 -3.2887650355617133 1105 64-65 -1.1330107752912468 1105 66-67 -1.7770876269520635 1105 68-69 -4.11593978593733 1105 70-71 -3.835335499700383 1105 72-73 -4.655678687089136 1105 74-75 -4.793274103343363 1105 76-77 -6.474013517204327 1105 78-79 -9.737844676166723 1105 80-81 -7.011556807908526 1105 82-83 -4.0885974528084255 1105 84-85 -3.259310764632186 1105 86-87 -3.207693945118155 1105 88-89 -3.2980850327953597 1105 90-91 -6.551981870248671 1105 92-93 -8.208445487815556 1105 94-95 -8.846729662761664 1105 96-97 -9.341037047386397 1105 98-99 -8.759645545271555 1105 100-101 -8.437650102697688 1106 1 0.2848263409690439 1106 2 0.35611662303168146 1106 3 -0.08651503645568681 1106 4 0.4652449520640829 1106 5 0.2778394191537856 1106 6 0.43166634720362396 1106 7 0.1530944469554214 1106 8 0.23440090758096233 1106 9 0.026626149358328632 1106 10-11 0.28704379612999276 1106 12-13 0.22146427330316243 1106 14-15 0.264398986590173 1106 16-17 0.28639842477569744 1106 18-19 0.34130845940246246 1106 20-21 0.6454781867710722 1106 22-23 0.5821957854330009 1106 24-25 0.26130770528389036 1106 26-27 0.6493163592525235 1106 28-29 0.4506303315245077 1106 30-31 0.39268044175732086 1106 32-33 0.7425273098773388 1106 34-35 0.7629349746506051 1106 36-37 0.921029457028439 1106 38-39 1.1707116953504553 1106 40-41 1.0591313294784825 1106 42-43 0.6286202528327536 1106 44-45 0.6655787904971433 1106 46-47 0.4771469674629998 1106 48-49 0.9159242368351137 1106 50-51 0.7310344200748595 1106 52-53 0.5714184813684575 1106 54-55 0.5187717910939149 1106 56-57 1.434829546978822 1106 58-59 -5.714219707856728 1106 60-61 -6.744178367632834 1106 62-63 -1.2163774206669622 1106 64-65 0.7536943726210446 1106 66-67 -0.11690944205386344 1106 68-69 -2.3045789301478905 1106 70-71 -3.277958707655964 1106 72-73 -4.658117749736185 1106 74-75 -4.630947033669303 1106 76-77 -6.217198258553889 1106 78-79 -8.686130273933138 1106 80-81 -5.144230836204791 1106 82-83 -2.216879599810966 1106 84-85 -1.4165009017216228 1106 86-87 -1.4329660849054626 1106 88-89 -1.198789302331388 1106 90-91 -4.884760989990177 1106 92-93 -7.078743822037723 1106 94-95 -8.245449743070349 1106 96-97 -9.339256766933406 1106 98-99 -8.972118488145728 1106 100-101 -8.716103219364879 1107 1 0.25152388612884025 1107 2 0.20836303340685802 1107 3 0.09671794177497262 1107 4 0.29451776355088555 1107 5 -0.06778355444509998 1107 6 0.414235437832005 1107 7 0.3291327362869012 1107 8 0.006100768627739228 1107 9 0.23673885585825616 1107 10-11 0.4104496939221818 1107 12-13 0.3783424576477685 1107 14-15 0.2741334315492665 1107 16-17 0.4192299825956809 1107 18-19 0.582809154168551 1107 20-21 0.45870190145379297 1107 22-23 0.5433043233363435 1107 24-25 0.7274417179440746 1107 26-27 1.0453561925086632 1107 28-29 0.832180431879614 1107 30-31 0.6540746057221227 1107 32-33 0.5159872450325125 1107 34-35 0.69249341219885 1107 36-37 0.8137730111429988 1107 38-39 1.099606245296421 1107 40-41 0.6198693254642791 1107 42-43 1.3832520268021895 1107 44-45 1.5906521269183216 1107 46-47 1.456365741586822 1107 48-49 1.3234585891587223 1107 50-51 1.163024538957064 1107 52-53 1.1911498384782284 1107 54-55 1.2419846983040443 1107 56-57 1.8303753243448853 1107 58-59 -4.869384135832643 1107 60-61 -7.378538410862724 1107 62-63 -1.440339131335481 1107 64-65 0.3511623361844194 1107 66-67 -0.6545503251787643 1107 68-69 -2.391308897725473 1107 70-71 -3.516649459547269 1107 72-73 -4.097480108853061 1107 74-75 -4.077033184665133 1107 76-77 -7.036203971075118 1107 78-79 -9.496613522350614 1107 80-81 -6.191683360521603 1107 82-83 -2.8694270754941513 1107 84-85 -1.6563109989888787 1107 86-87 -1.4964213882866595 1107 88-89 -1.1871249515517057 1107 90-91 -5.395908126009939 1107 92-93 -8.848606412743296 1107 94-95 -9.057970972034376 1107 96-97 -9.6510909494253 1107 98-99 -9.06862921842209 1107 100-101 -8.295700255029537 1108 1 -0.3204383294143085 1108 2 -0.37944289188296665 1108 3 -0.015390259169638654 1108 4 -0.048290308583077035 1108 5 -0.26477797265033587 1108 6 -0.25774109000541046 1108 7 -0.004587028991473119 1108 8 -0.1427184670957402 1108 9 -0.1902541311175554 1108 10-11 -0.012700442044611293 1108 12-13 -0.19430674801990477 1108 14-15 -0.1182810524925273 1108 16-17 0.03435807762930665 1108 18-19 -0.3953574409366425 1108 20-21 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0.5602638506883721 2311 18-19 0.023985624756790003 2311 20-21 0.2211617945019242 2311 22-23 -0.08421795830358292 2311 24-25 0.25639002453944215 2311 26-27 -0.1641625240688782 2311 28-29 0.20590592207569358 2311 30-31 0.12819225278094137 2311 32-33 -0.09791649828300564 2311 34-35 -0.2251179960007974 2311 36-37 -0.1893642437589591 2311 38-39 -0.2666326138836226 2311 40-41 -0.9454069668708343 2311 42-43 -1.4039137485988817 2311 44-45 -1.5770840584649228 2311 46-47 -1.0566110855076545 2311 48-49 -0.5300886479179248 2311 50-51 -0.37330344678269256 2311 52-53 -0.3425400010939619 2311 54-55 -0.41284596123249884 2311 56-57 0.2990027753252775 2311 58-59 3.4333966128304567 2311 60-61 2.4649196996542067 2311 62-63 0.6497499952242336 2311 64-65 0.5043887176460977 2311 66-67 -0.5662437298133476 2311 68-69 -2.4916297533404457 2311 70-71 -0.46591862175760923 2311 72-73 -1.3246102336302439 2311 74-75 0.7189026442118713 2311 76-77 3.3090723212600004 2311 78-79 3.9206413796101707 2311 80-81 2.239011826644173 2311 82-83 1.2471504646127975 2311 84-85 1.0610152042196788 2311 86-87 1.1004770074352663 2311 88-89 0.8174383283056912 2311 90-91 2.179992052243179 2311 92-93 4.039022820768292 2311 94-95 6.783455052921058 2311 96-97 7.090299424906249 2311 98-99 7.675827109563649 2311 100-101 7.406652686146934 2312 1 -0.10695362252167584 2312 2 -0.051152537527400455 2312 3 -0.18217479178904128 2312 4 0.0889814313709536 2312 5 -0.11207421188454703 2312 6 -0.06857886435945915 2312 7 -0.08170924756546327 2312 8 0.36734644336822697 2312 9 0.2662659608178828 2312 10-11 -0.05241074437078197 2312 12-13 0.17349816699032772 2312 14-15 -0.06127602635154261 2312 16-17 0.04944912907780008 2312 18-19 0.2613558669713214 2312 20-21 0.418909513910549 2312 22-23 0.047225891963798006 2312 24-25 0.3538546360524961 2312 26-27 0.5962212444117796 2312 28-29 0.2614768563317469 2312 30-31 -0.027932314693110527 2312 32-33 0.0889976256553453 2312 34-35 0.421443815889738 2312 36-37 0.33015132717529383 2312 38-39 0.5215208935086437 2312 40-41 0.09057290101214477 2312 42-43 -0.1963327482843198 2312 44-45 -0.44418062971374894 2312 46-47 -0.9466331050106405 2312 48-49 -0.6316740521330857 2312 50-51 -0.41079322344201685 2312 52-53 -0.26905777397851693 2312 54-55 -0.00657354852410208 2312 56-57 1.0965806299965593 2312 58-59 3.711930743060094 2312 60-61 3.0978168371188204 2312 62-63 1.015946912493817 2312 64-65 0.7706548390679266 2312 66-67 -0.056165088731244595 2312 68-69 -1.4795441918431198 2312 70-71 0.41299298566610965 2312 72-73 5.162612163349939 2312 74-75 4.321236711528639 2312 76-77 4.211431553723038 2312 78-79 4.187354182378336 2312 80-81 2.677520791727531 2312 82-83 1.4838601217376777 2312 84-85 0.9890177207343065 2312 86-87 0.9516754975264909 2312 88-89 1.2825405617124197 2312 90-91 2.386906176181526 2312 92-93 3.443665790255551 2312 94-95 6.478591888403917 2312 96-97 7.084203168221759 2312 98-99 7.517991312143074 2312 100-101 7.144092132513713 2313 1 -0.14346985604888118 2313 2 -0.21466575257562326 2313 3 -0.31600629685664927 2313 4 -0.16238670036234026 2313 5 -0.40921033792444916 2313 6 -0.1798822092268182 2313 7 -0.538702057204965 2313 8 -0.5282338162700455 2313 9 -0.1428606435159594 2313 10-11 -0.12388489097561006 2313 12-13 -0.2834639670497765 2313 14-15 -0.834877832530843 2313 16-17 -0.5755968843212997 2313 18-19 -0.5955179213424984 2313 20-21 -0.4328291790635177 2313 22-23 -0.5319355598509716 2313 24-25 -0.27522828622780793 2313 26-27 -0.2062708412835761 2313 28-29 -0.7239522339526729 2313 30-31 -0.2106729954460107 2313 32-33 -0.023416175910334402 2313 34-35 -0.13228756402142494 2313 36-37 -0.5481585700000977 2313 38-39 -0.7360007634645314 2313 40-41 -0.7115449423705158 2313 42-43 -0.747014973615002 2313 44-45 -1.1020679398317839 2313 46-47 -1.1837677586217765 2313 48-49 -1.547567693694841 2313 50-51 -0.7781519316311751 2313 52-53 -0.9875109875543089 2313 54-55 -0.8934971540113494 2313 56-57 -0.6407135367314609 2313 58-59 2.8819717256608897 2313 60-61 2.5862091903054 2313 62-63 0.2017551531868378 2313 64-65 -0.03559193999413779 2313 66-67 0.8875022405283666 2313 68-69 1.2033928127459514 2313 70-71 2.4995165813371685 2313 72-73 6.497743086808184 2313 74-75 7.218148292180926 2313 76-77 6.402657105270311 2313 78-79 4.878230032092439 2313 80-81 2.514240711234759 2313 82-83 1.275358072607638 2313 84-85 0.9319242951287698 2313 86-87 1.32564786494655 2313 88-89 1.2543435507428278 2313 90-91 2.0900952114952744 2313 92-93 3.547784909743143 2313 94-95 6.903835452663161 2313 96-97 6.967494782739905 2313 98-99 7.070127560885375 2313 100-101 6.850127863451895 2314 1 -0.26324369922697954 2314 2 -0.520349430599726 2314 3 -0.2676999932311972 2314 4 -0.5247335733475111 2314 5 -0.8595153520592547 2314 6 -0.4083204376650471 2314 7 -0.6984005147757628 2314 8 -0.5380785814339575 2314 9 -0.593934392462053 2314 10-11 -0.5608858332956999 2314 12-13 -0.8148085090326092 2314 14-15 -0.8494713949967405 2314 16-17 -1.0618340514095337 2314 18-19 -0.7718185710474117 2314 20-21 -0.9894675761274456 2314 22-23 -0.7599522240378462 2314 24-25 -0.5126309544815442 2314 26-27 -1.073812873719227 2314 28-29 -1.028569602399834 2314 30-31 -0.60915040456171 2314 32-33 -0.7856787360452344 2314 34-35 -1.484628485511287 2314 36-37 -1.2265670409617613 2314 38-39 -1.1429962502472506 2314 40-41 -2.274358015821882 2314 42-43 -2.115102559787694 2314 44-45 -1.7186924500733127 2314 46-47 -2.058079616976187 2314 48-49 -1.5142145220437975 2314 50-51 -0.9751216286008741 2314 52-53 -1.3865959451499066 2314 54-55 -1.3329158913024273 2314 56-57 -0.36358463726213586 2314 58-59 2.7433266827605287 2314 60-61 2.287716902451411 2314 62-63 0.3062534917277304 2314 64-65 0.13075235400973284 2314 66-67 0.12732270375308374 2314 68-69 -0.2056856973963921 2314 70-71 1.1635918677528796 2314 72-73 5.180354801334792 2314 74-75 6.562888658197883 2314 76-77 5.5080233702110455 2314 78-79 3.3399420789108696 2314 80-81 1.353277560909909 2314 82-83 0.8660315834939141 2314 84-85 0.24136485456932988 2314 86-87 -0.02141110445284511 2314 88-89 0.3455502164175783 2314 90-91 1.2814605837117128 2314 92-93 2.6202815620270314 2314 94-95 5.1875109969770055 2314 96-97 6.083586138192963 2314 98-99 6.207855081591621 2314 100-101 5.785603735097979 2315 1 -0.6886429268650112 2315 2 -0.6046308208512663 2315 3 -0.5971802145024618 2315 4 -0.585113491936383 2315 5 -1.0985561619955817 2315 6 -0.9609767365900055 2315 7 -0.6026641615767261 2315 8 -0.9410897493704979 2315 9 -1.264036678235982 2315 10-11 -1.1996761474591509 2315 12-13 -1.0954942412108792 2315 14-15 -1.0798100861563213 2315 16-17 -1.2799871669507041 2315 18-19 -1.3334770618103775 2315 20-21 -1.2697880019702552 2315 22-23 -0.7754255566753585 2315 24-25 -1.1711608574144279 2315 26-27 -1.2000783992384854 2315 28-29 -0.49103437643177017 2315 30-31 -0.9200913293086117 2315 32-33 -1.0475772852572192 2315 34-35 -1.3451858386059499 2315 36-37 -1.1868269303261272 2315 38-39 -1.1775959788768304 2315 40-41 -1.073948350859979 2315 42-43 -1.0969938028729658 2315 44-45 -0.8194517653848692 2315 46-47 -0.7604984667830976 2315 48-49 -0.2934129355705686 2315 50-51 0.4127684663787754 2315 52-53 0.4406011115247601 2315 54-55 0.1563467117661439 2315 56-57 1.300868446967069 2315 58-59 3.916612352314857 2315 60-61 3.5630404594913863 2315 62-63 1.8879385976665688 2315 64-65 1.6919803051630566 2315 66-67 3.1428743163196238 2315 68-69 6.282637546523867 2315 70-71 4.316720455582962 2315 72-73 7.709331421729322 2315 74-75 8.262606850453393 2315 76-77 7.089330123159591 2315 78-79 5.252581678117632 2315 80-81 3.16955456748542 2315 82-83 1.849932011424194 2315 84-85 1.5681183249795154 2315 86-87 1.6751106573674228 2315 88-89 1.6431574599203422 2315 90-91 3.05257007123911 2315 92-93 4.108161219682806 2315 94-95 7.565368214386462 2315 96-97 8.085190876738004 2315 98-99 7.973615440635562 2315 100-101 7.078070596594692 2316 1 -0.3539161846363079 2316 2 -0.5654096335546654 2316 3 -0.40721836959167845 2316 4 -0.3559024045163426 2316 5 -0.7056429087131235 2316 6 -0.7162230613571481 2316 7 -0.7616530517125142 2316 8 -0.7264954277455402 2316 9 -0.5036562495523285 2316 10-11 -1.0433244473132604 2316 12-13 -0.6073054857401132 2316 14-15 -0.8172273232789848 2316 16-17 -1.2212535792501171 2316 18-19 -1.3673677587018531 2316 20-21 -0.7587903586081239 2316 22-23 -1.2279841920698118 2316 24-25 -2.4327897430613845 2316 26-27 -1.5334584912595801 2316 28-29 -1.7127406944656656 2316 30-31 -0.9179731607528936 2316 32-33 -0.7150388496158726 2316 34-35 -0.9647010445312105 2316 36-37 -1.1116950708266344 2316 38-39 -1.1190728052711734 2316 40-41 -1.0729804460232586 2316 42-43 -1.3086984375940958 2316 44-45 -1.0929760611990318 2316 46-47 -0.6867272850975397 2316 48-49 -0.14401892816399453 2316 50-51 -0.46110932511488656 2316 52-53 -0.5117471097747561 2316 54-55 0.06400297930065335 2316 56-57 0.7802057828440745 2316 58-59 4.243287248510569 2316 60-61 3.8173188794218085 2316 62-63 1.167747261116233 2316 64-65 1.7020100436884746 2316 66-67 5.002710473877698 2316 68-69 7.648828209749096 2316 70-71 4.763794296902676 2316 72-73 7.791322780723823 2316 74-75 8.12648295863429 2316 76-77 6.59236008612671 2316 78-79 4.535378221715433 2316 80-81 2.3606386209025487 2316 82-83 1.0432885370666583 2316 84-85 1.0766611372340336 2316 86-87 0.7186383198071056 2316 88-89 0.9241163870890112 2316 90-91 2.0039223324892497 2316 92-93 3.8506564502966576 2316 94-95 6.3586293523058846 2316 96-97 7.336635720189918 2316 98-99 7.342457321457019 2316 100-101 6.293043663590542 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 10.0 5 46.0 6 132.0 7 238.0 8 448.0 9 802.0 10 1430.0 11 2288.0 12 3097.0 13 3859.0 14 4498.0 15 5536.0 16 6679.0 17 7856.0 18 7595.0 19 7384.0 20 7461.0 21 7553.0 22 6809.0 23 6196.0 24 6080.0 25 5469.0 26 5975.0 27 8209.0 28 11458.0 29 14127.0 30 16385.0 31 17374.0 32 17808.0 33 18269.0 34 18128.0 35 16861.0 36 12204.0 37 6276.0 38 1137.0 39 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.743215227306514 20.509219780477693 14.998865618863585 13.748699373352213 2 14.003848349980835 29.51262055425626 29.86929689394354 26.614234201819365 3 31.853700125931777 26.14648759845753 31.68122834325405 10.318583932356646 4 11.176247443322918 19.089725188988787 39.42933794284776 30.304689424840536 5 7.835080593090009 45.301573512089945 32.948270936789505 13.915074958030546 6 22.81514100342976 38.6358392353629 12.46485180072193 26.084167960485406 7 17.573121942253316 38.78523130350446 21.90392760350876 21.73771915073347 8 23.69663242120742 22.29109552879708 19.5676233755568 34.4446486744387 9 16.825770522833132 25.74236516583693 27.933188112771457 29.49867619855848 10-11 25.521210153967687 24.70248686953223 23.205046479704972 26.571256496795108 12-13 34.265730163510014 24.460604685905132 23.236528316054173 18.03713683453069 14-15 24.165778590354027 28.027492887242012 32.80667963746933 15.00004888493464 16-17 16.165825195637126 31.517815590743947 31.21707606089878 21.099283152720147 18-19 15.337730421842826 36.10624971890833 29.65942444158084 18.896595417668006 20-21 20.71778586876181 26.56050183220377 26.135008193099026 26.5867041059354 22-23 16.369772744160215 28.733921776435945 31.278279879391324 23.618025600012515 24-25 25.282261060683688 29.29023124484265 26.92322733796631 18.504280356507355 26-27 20.815555546864918 23.577157874565412 38.8149532856478 16.792333292921864 28-29 14.407034861438103 37.16337241589688 30.835008486574004 17.594584236091013 30-31 17.50357866412184 28.046753388975365 28.985223832729716 25.464444114173073 32-33 19.20497297635529 29.071145317596265 33.76762794190112 17.956253764147327 34-35 23.21079389910051 27.588971450919047 30.24951114587407 18.950723504106374 36-37 17.93390886652298 25.604055592281544 38.013745516278306 18.44829002491717 38-39 17.501799349105017 29.532012767555386 34.59835398673176 18.367833896607834 40-41 16.44643939838448 31.40742044632205 32.95448766844648 19.191652486846994 42-43 15.955813466800448 30.866421403243187 27.66098101457694 25.516784115379426 44-45 18.357471860117155 25.124343565721663 30.95866297661774 25.559521597543444 46-47 23.396469239826473 29.42665242783769 31.50712528213614 15.669753050199695 48-49 19.773707423452674 26.952286885005428 32.14841037777365 21.12559531376825 50-51 10.446777467895938 29.256844238088348 39.37544249431847 20.920935799697247 52-53 16.76529949699212 32.08709155056285 28.773536935485133 22.374072016959893 54-55 13.64241726134717 25.42344706740538 32.173763393756396 28.760372277491058 56-57 20.966412144973575 23.80877776711559 28.848620628203143 26.376189459707692 58-59 22.64053564780251 24.505414195297128 31.270745222566077 21.58330493433429 60-61 19.595483758357148 28.470483022908727 32.66312736201517 19.270905856718958 62-63 14.423486806856445 29.448457377953158 32.26189964059586 23.866156174594547 64-65 13.68928502895991 35.06146393424005 28.093854389805482 23.155396646994554 66-67 18.406218512729783 26.739485731060036 25.33163285545409 29.522662900756092 68-69 19.031303191008345 25.474612000795794 27.87223909151014 27.621845716685723 70-71 19.398068189087187 25.538524275570907 28.72469295720591 26.338714578135995 72-73 20.55333292177546 21.587073365501226 32.77899143096681 25.080602281756505 74-75 16.436854646544877 29.70214455917395 28.40369340746624 25.457307386814932 76-77 16.520405117144332 24.46380100785048 28.385679745526648 30.630114129478542 78-79 17.618245498307694 25.146849797001753 29.756323572140946 27.47858113254961 80-81 20.751890872969316 23.192427122196932 27.452268656482936 28.603413348350813 82-83 17.069250398412215 22.456761275310175 32.636560064919195 27.837428261358415 84-85 17.165422384563342 25.316284908663565 29.50923532379362 28.009057382979474 86-87 16.881212223132593 26.440629576977305 28.013594294776407 28.6645639051137 88-89 16.024059162387612 25.996957407617433 24.480354160881962 33.498629269112996 90-91 18.188712670663097 21.293227955139145 26.724659947598322 33.793399426599436 92-93 20.666430343849697 24.330739657775837 27.577501806694464 27.42532819168 94-95 18.69366559122535 22.50920462895294 29.180505794113394 29.61662398570832 96-97 14.69099360744696 23.73816862957046 31.74024338819169 29.83059437479089 98-99 17.165080743870178 25.45307379476448 26.336518340111343 31.045327121253997 100-101 14.885130572698294 22.602895683170804 28.506147830593115 34.00582591353779 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1465.0 1 994.0 2 477.0 3 429.5 4 408.0 5 427.5 6 524.5 7 677.0 8 821.5 9 883.5 10 829.5 11 767.0 12 737.5 13 708.5 14 715.0 15 740.0 16 739.5 17 725.0 18 760.0 19 787.5 20 856.0 21 951.0 22 1047.0 23 1148.0 24 1240.5 25 1372.5 26 1541.5 27 1755.0 28 1910.5 29 2125.0 30 2407.0 31 2590.0 32 2806.5 33 3096.0 34 3263.5 35 3348.0 36 3529.5 37 3668.0 38 3554.0 39 3338.0 40 3167.0 41 3161.5 42 3491.0 43 4387.5 44 6311.5 45 10097.5 46 16090.5 47 23939.5 48 29024.5 49 27341.0 50 22920.5 51 17975.0 52 12021.5 53 6702.5 54 3340.0 55 1791.0 56 1099.0 57 767.5 58 568.0 59 438.0 60 354.0 61 283.0 62 239.5 63 192.0 64 138.5 65 105.0 66 74.5 67 55.5 68 47.0 69 36.0 70 30.5 71 20.0 72 13.5 73 14.5 74 13.0 75 7.5 76 1.5 77 2.0 78 3.5 79 3.5 80 1.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022291486607509493 2 0.0035197084117120254 3 0.0035197084117120254 4 0.0 5 0.06257259398599156 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.9553935620622361E-4 16-17 0.0 18-19 0.0013687754934435653 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0019553935620622363 30-31 0.008212652960661393 32-33 3.9107871241244723E-4 34-35 0.0011732361372373417 36-37 0.021900407895097045 38-39 0.0215093291826846 40-41 0.02268256531992194 42-43 0.02326918338854061 44-45 0.02366026210095306 46-47 0.025029037594396623 48-49 0.02366026210095306 50-51 0.01955393562062236 52-53 0.01603422720891034 54-55 0.013101136865816982 56-57 0.15154300105982332 58-59 0.7350324399791947 60-61 0.2958510459400163 62-63 7.821574248248945E-4 64-65 0.13687754934435653 66-67 1.4145317027958217 68-69 2.6962921827276176 70-71 2.1802638216993935 72-73 1.8085435055513621 74-75 1.5263802145457817 76-77 1.0905229895621091 78-79 0.39929136537310866 80-81 3.9107871241244723E-4 82-83 1.9553935620622361E-4 84-85 0.0 86-87 0.0029330903430933543 88-89 0.0 90-91 0.21841746088235178 92-93 0.15740918174601 94-95 1.2704191972718348 96-97 1.229355932468528 98-99 0.9514945072994841 100-101 0.38286605945178587 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 255703.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.945002600673437 #Duplication Level Percentage of deduplicated Percentage of total 1 80.7233414866404 16.907505973727332 2 8.209944545064138 3.439146196955061 3 3.383311238493568 2.1259038806740636 4 1.839162014302519 1.5408501269050423 5 1.1595122953115373 1.2142994020406488 6 0.7991485706817036 1.0042901334751644 7 0.5732210542039323 0.8404281529743491 8 0.48172974587822315 0.807186462419291 9 0.3211531639188155 0.6053898468144684 >10 2.0445506656459473 8.017504683167582 >50 0.2221931773624363 3.2627696976570473 >100 0.1643109210747428 6.375756248460128 >500 0.02053886513434285 3.369143107433233 >1k 0.046679238941688295 29.24838582261452 >5k 0.0074686782306701265 9.485614169563908 >10k+ 0.0037343391153350633 11.755826095118165 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17677 6.913098399314831 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 12383 4.842727695803335 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 8247 3.225226141265453 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5636 2.2041196231565525 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5358 2.0953997411058927 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5014 1.9608686640360105 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4947 1.9346663903043764 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4784 1.8709205601811476 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4665 1.8243821934040665 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4612 1.8036550216462068 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4467 1.7469486083464019 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3869 1.5130835383237584 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3721 1.4552038888867163 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3702 1.4477733933508798 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 3656 1.4297837725799072 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3447 1.3480483216857055 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3399 1.329276543489908 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3220 1.25927345396808 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3153 1.2330711802364462 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2954 1.1552465164663692 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2790 1.0911096076307278 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2669 1.0437890834288217 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2489 0.9733949151945812 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2084 0.8150080366675401 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1863 0.7285796412243892 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1817 0.7105900204534167 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 1796 0.7023773674927553 RNA PCR Primer, Index 26 (100% over 22bp) CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1496 0.5850537537690211 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1076 0.4208006945557933 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1060 0.41454343515719405 No Hit ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT 1053 0.41180588417030695 RNA PCR Primer, Index 26 (100% over 24bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 982 0.3840392955890232 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 960 0.37543556391594934 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 937 0.36644075353046307 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 837 0.32733288228921836 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 823 0.3218577803154441 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 799 0.31247189121754537 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 739 0.28900716847279856 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 652 0.2549833204929156 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 648 0.2534190056432658 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 630 0.24637958881984176 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 608 0.23777585714676794 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 495 0.1935839626441614 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 485 0.18967317552003693 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 438 0.17129247603665188 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 419 0.16386198050081538 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCCTGTC 390 0.15252069784085442 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 384 0.15017422556637974 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCCTGTC 371 0.14509020230501793 No Hit CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT 368 0.1439169661677806 RNA PCR Primer, Index 26 (100% over 27bp) GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 340 0.13296676222023207 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 313 0.12240763698509599 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 302 0.11810577114855907 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 302 0.11810577114855907 No Hit CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG 295 0.11536822016167195 RNA PCR Primer, Index 26 (96% over 27bp) TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC 287 0.11223959046237236 RNA PCR Primer, Index 26 (95% over 22bp) GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 276 0.10793772462583545 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 275 0.107546645913423 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 271 0.1059823310637732 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.9107871241244723E-4 0.0 16-17 0.0 0.0 0.0 3.9107871241244723E-4 0.0 18-19 0.0 0.0 0.0 5.866180686186709E-4 0.0 20-21 0.0 0.0 0.0 0.004106326480330696 0.0 22-23 0.0 0.0 0.0 0.013296676222023206 0.0 24-25 0.0 0.0 0.0 0.035783702185738925 0.0 26-27 0.0 0.0 0.0 0.08623285608694461 0.0 28-29 0.0 0.0 0.0 0.24422865590157333 0.0 30-31 0.0 0.0 0.0 0.5570916258315312 0.0 32-33 0.0 0.0 0.0 1.1417543008881397 0.0 34-35 0.0 0.0 0.0 1.9600865066111857 0.0 36-37 0.0 0.0 0.0 2.9340680398743855 0.0 38-39 0.0 0.0 0.0 4.131942135993711 0.0 40-41 0.0 0.0 0.0 5.2343930262844 0.0 42-43 0.0 0.0 0.0 6.335866219794058 0.0 44-45 0.0 0.0 0.0 7.432255390042354 0.0 46-47 0.0 0.0 0.0 8.709518464781407 0.0 48-49 0.0 0.0 0.0 9.498324227717312 0.0 50-51 0.0 0.0 0.0 10.2865433725846 0.0 52-53 0.0 0.0 0.0 10.991071672995623 0.0 54-55 0.0 0.0 0.0 11.74722236344509 0.0 56-57 0.0 0.0 0.0 12.655698212379207 0.0 58-59 0.0 0.0 0.0 13.043452755736148 0.0 60-61 0.0 0.0 0.0 13.305279953696282 0.0 62-63 0.0 0.0 0.0 13.531323449470674 0.0 64-65 0.0 0.0 0.0 13.770272542754679 0.0 66-67 0.0 0.0 0.0 13.983214901663256 0.0 68-69 0.0 0.0 0.0 14.257165539708176 0.0 70-71 0.0 0.0 0.0 14.64687547662718 0.0 72-73 0.0 0.0 0.0 15.020746725693481 0.0 74-75 0.0 0.0 0.0 15.606582636887326 0.0 76-77 0.0 0.0 0.0 16.164065341431268 0.0 78-79 0.0 0.0 0.0 17.5537635459889 0.0 80-81 0.0 0.0 0.0 18.794265221761187 0.0 82-83 0.0 0.0 0.0 19.564690285213707 0.0 84-85 0.0 0.0 0.0 20.376569692181945 0.0 86-87 0.0 0.0 0.0 20.97785321251608 0.0 88-89 0.0 0.0 0.0 21.663414195375104 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTTATG 25 0.0051618936 56.59092 1 ACCGGGG 15 0.009357927 48.69327 64-65 GCGAACT 15 0.009357927 48.69327 66-67 TGGTGCT 15 0.009357927 48.69327 66-67 TCAGTTG 20 4.8511702E-4 48.51681 70-71 TTACGCG 75 0.0 42.03521 62-63 CAACATA 30 7.324896E-5 40.418472 62-63 ATACCGG 30 7.324896E-5 40.418472 62-63 GATGCAG 30 7.478315E-5 40.276608 72-73 AACGTAG 60 8.913048E-11 39.469177 86-87 TACGCGT 50 2.4307155E-8 38.954617 64-65 CGGCTGA 25 0.001429575 38.954617 68-69 GATAGTA 25 0.001429575 38.954617 68-69 AGAGAGT 25 0.001429575 38.954617 68-69 CAACTGA 50 2.5165718E-8 38.80173 62-63 ACGTAGA 50 2.7705028E-8 38.38073 88-89 CGCAGTT 40 1.00531E-5 35.981934 88-89 GTACCCA 105 4.9365553E-8 35.930744 1 TTAGTAA 35 1.7733363E-4 34.780907 68-69 CGGTCAT 35 1.8115886E-4 34.65486 70-71 >>END_MODULE