FastQCFastQC Report
Fri 27 May 2016
SRR939745_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939745_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427991
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135850.9513365280313392No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98200.6876794041419029No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68910.48256606659285667No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42590.29825117945421226No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA34930.24460938479304142No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28140.19706006550461452No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA27110.18984713489090616No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22880.16022509945790975No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20300.14215775869735875No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19110.133824372842686No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16210.11351612160020617No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14760.10336199597896627No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG128900.025.3152372
GTACATG127950.024.7247541
GTATCAA197050.023.8771061
ACATGGG132750.023.5490573
GAGTACT96450.023.31098712-13
GTCCAAG7750.022.6436521
GTAAGGT2955.456968E-1222.5135484
AGTACTT94750.022.12725812-13
GTACTTT103200.021.83569514-15
CATGGGA81600.021.626724
GATGTGC6500.021.1644658
TATCAAC227850.021.0657982
ATGGGAG21300.020.7136425
ATCAACG232900.020.5311683
ACTTTTT116150.020.5290616-17
TCAACGC234950.020.4537014
CATGGGG45100.020.4062424
CAACGCA236400.020.3088875
AACGCAG240850.019.9927486
GGTATCA147150.019.9193271
Produced by FastQC (version 0.11.5)