FastQCFastQC Report
Fri 27 May 2016
SRR939745_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939745_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427991
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148411.0392922644470448No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115500.808828627071179No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90870.636348548415221No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52190.36547849391207643No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA37640.26358709543687603No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33210.2325644909526741No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA33060.23151406416426992No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28750.20133180111079133No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25920.1815137490362334No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT21330.1493706893110671No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19050.13340420212732432No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18540.1298327510467503No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16300.11414637767324864No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA15550.108894243731228No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15350.10749367468002251No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG14890.10427236586224983No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG14420.10098102859191689No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG8000.027.1036472
GTACATG130450.025.4084191
TACATGG131050.025.1420122
GTATCAA185800.024.0055581
ATGGGAG24750.023.8056185
ACATGGG131600.023.6750133
AGTACTT96600.022.36192112-13
GTGTACG1703.3596807E-622.1874521
TACGCGG550.002473695722.16187164-65
GAGTACT95350.022.08684712-13
TGCACCG4700.022.063255
CATGGGA82300.021.7062114
AGGTAAG2901.1823431E-1021.1302782
GGTATCA138400.021.1213651
GTACTTT101200.020.99667714-15
CATGGGC14500.020.804474
TACTTTT104800.020.4777214-15
ATCAACG219100.020.3298473
TCAACGC219850.020.2819334
TATCAAC220600.020.2350522
Produced by FastQC (version 0.11.5)