##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939745_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1427991 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.42153907132468 31.0 31.0 34.0 30.0 34.0 2 31.60484975045361 31.0 31.0 34.0 30.0 34.0 3 31.649454373311876 31.0 31.0 34.0 30.0 34.0 4 35.460662567201055 37.0 35.0 37.0 33.0 37.0 5 35.21637811442789 37.0 35.0 37.0 32.0 37.0 6 35.26385600469471 37.0 35.0 37.0 32.0 37.0 7 35.17553752089474 37.0 35.0 37.0 32.0 37.0 8 35.12845809252299 37.0 35.0 37.0 32.0 37.0 9 36.69535102111988 39.0 35.0 39.0 32.0 39.0 10-11 36.55577486132616 38.5 35.0 39.0 32.0 39.0 12-13 36.474755443136544 38.0 35.0 39.0 32.0 39.0 14-15 37.40800782357872 39.0 36.0 41.0 32.0 41.0 16-17 37.37318197383597 39.0 36.0 41.0 32.0 41.0 18-19 37.4293759554507 39.0 36.0 41.0 32.0 41.0 20-21 37.39967058615916 39.0 36.0 41.0 32.0 41.0 22-23 37.2430614058492 39.0 36.0 40.5 31.0 41.0 24-25 37.046289857569136 39.0 36.0 40.0 31.0 41.0 26-27 36.71517922732006 39.0 35.0 40.0 30.5 41.0 28-29 36.45833026958853 38.0 35.0 40.0 30.0 41.0 30-31 36.122447550439745 38.0 35.0 40.0 30.0 41.0 32-33 35.94987188294604 38.0 35.0 40.0 30.0 41.0 34-35 35.903193717607465 38.0 35.0 40.0 30.0 41.0 36-37 35.777071424119626 38.0 35.0 40.0 29.0 41.0 38-39 35.57693991068571 38.0 34.0 40.0 28.5 41.0 40-41 35.328810895866994 38.0 34.0 40.0 27.0 41.0 42-43 35.02921201884325 38.0 34.0 40.0 26.5 41.0 44-45 34.63250643736551 38.0 33.0 40.0 24.5 41.0 46-47 34.12636669278728 38.0 33.0 40.0 23.5 41.0 48-49 33.6558896379599 37.0 33.0 40.0 21.5 41.0 50-51 33.318589192789034 37.0 33.0 40.0 20.0 41.0 52-53 32.976572681480484 37.0 32.0 40.0 18.5 41.0 54-55 32.62173010894326 36.5 32.0 40.0 14.5 41.0 56-57 31.25494558439094 35.5 29.5 39.0 5.5 40.5 58-59 27.81599954061335 33.5 20.0 38.0 2.0 40.0 60-61 27.767250283790304 33.5 19.0 38.0 2.0 40.0 62-63 29.656522345028783 34.0 27.5 38.0 2.0 40.0 64-65 29.20498973733028 34.0 28.0 38.0 2.0 40.0 66-67 23.91555303919983 27.5 16.0 35.0 2.0 38.0 68-69 16.568035792942673 13.0 7.0 28.5 2.0 34.5 70-71 15.494652277220235 12.0 6.5 25.5 2.0 33.0 72-73 17.4530763849352 15.5 7.0 29.0 2.0 34.0 74-75 18.1213974737936 17.5 6.5 30.0 2.0 34.5 76-77 19.004534342303277 21.0 7.0 30.5 2.0 34.0 78-79 21.903353032337037 27.5 7.5 33.5 2.0 35.0 80-81 24.4402128584844 30.5 14.5 34.0 2.0 36.0 82-83 25.456353366372756 32.0 20.0 35.0 2.0 36.0 84-85 25.47591336359963 33.0 20.0 35.0 2.0 35.0 86-87 25.198249848913612 32.5 18.5 35.0 2.0 35.0 88-89 24.75816199121703 32.0 11.5 35.0 2.0 35.0 90-91 23.116794153464554 31.5 2.0 34.0 2.0 35.0 92-93 21.032033815339172 29.5 2.0 34.0 2.0 35.0 94-95 16.839049055631303 17.0 2.0 33.0 2.0 35.0 96-97 15.584907397875757 6.5 2.0 31.5 2.0 34.5 98-99 14.554496491924668 2.0 2.0 31.0 2.0 34.0 100-101 14.002603657866192 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3794094073700762 1101 2 0.396129428642066 1101 3 0.41117706361430706 1101 4 0.23903158967814875 1101 5 0.2857609460923598 1101 6 0.31167160613868106 1101 7 0.4204590689396852 1101 8 0.3798571390420378 1101 9 0.5539175580481412 1101 10-11 0.4988123018282309 1101 12-13 0.6367674175825115 1101 14-15 0.5546187582897133 1101 16-17 0.3512390012168396 1101 18-19 0.5791075735419255 1101 20-21 0.6776448142010096 1101 22-23 0.5405670599862873 1101 24-25 0.4334524285195229 1101 26-27 0.40362494590440434 1101 28-29 0.42988410091765417 1101 30-31 0.5246074981511981 1101 32-33 0.5198847982413639 1101 34-35 0.8233451568592827 1101 36-37 0.6346512476501687 1101 38-39 0.4161812080153595 1101 40-41 0.591425816047888 1101 42-43 0.7734769290925243 1101 44-45 0.7341822338794017 1101 46-47 0.8217777530817685 1101 48-49 0.782795501680063 1101 50-51 0.5981434194645985 1101 52-53 0.5988175518912371 1101 54-55 0.8201073827819201 1101 56-57 1.2858712805112162 1101 58-59 -7.027682705881457 1101 60-61 -8.95172917166678 1101 62-63 -2.3132404401342477 1101 64-65 0.3955405096209148 1101 66-67 -0.8142374798695755 1101 68-69 -3.099718958243086 1101 70-71 -3.5444216096967267 1101 72-73 -5.96435912346649 1101 74-75 -6.869424868282444 1101 76-77 -7.768191622663004 1101 78-79 -10.591959393818879 1101 80-81 -6.949053446392821 1101 82-83 -3.428718877929686 1101 84-85 -2.6936547546020186 1101 86-87 -2.3648132534158712 1101 88-89 -2.538108335717787 1101 90-91 -7.482272670851479 1101 92-93 -12.726978725765296 1101 94-95 -11.176553833913143 1101 96-97 -11.292823194089667 1101 98-99 -10.84998557219938 1101 100-101 -10.694750179953529 1102 1 0.4662913994307054 1102 2 0.49491484004589026 1102 3 0.4456242397240082 1102 4 0.3573891320816145 1102 5 0.4273445350494143 1102 6 0.42957240909790784 1102 7 0.4830210952839664 1102 8 0.3780414587821994 1102 9 0.5979619012455402 1102 10-11 0.6764952231562731 1102 12-13 0.6202881456590177 1102 14-15 0.7175394096791052 1102 16-17 0.9049763703976339 1102 18-19 0.7977617146462208 1102 20-21 0.7968481703901062 1102 22-23 0.8360943966878409 1102 24-25 0.8792301261016178 1102 26-27 0.9260333724652057 1102 28-29 0.7036937589833343 1102 30-31 0.8412209725286814 1102 32-33 0.6234857094647452 1102 34-35 0.6099012286745094 1102 36-37 0.6628704149183093 1102 38-39 0.5192661728295036 1102 40-41 0.5234844140269601 1102 42-43 0.638389190009157 1102 44-45 0.7130960962034649 1102 46-47 0.5637397027389355 1102 48-49 0.5637128149243793 1102 50-51 0.5313241303992768 1102 52-53 0.7300511994913776 1102 54-55 0.5410367629695756 1102 56-57 0.908852604942826 1102 58-59 -8.579787654455973 1102 60-61 -9.721650905697818 1102 62-63 -2.766844734612775 1102 64-65 -0.07825617930005535 1102 66-67 -1.222700692608658 1102 68-69 -3.731550314251745 1102 70-71 -4.305969787249955 1102 72-73 -6.619918375541554 1102 74-75 -7.170872901483456 1102 76-77 -7.930923375640269 1102 78-79 -10.826559944161716 1102 80-81 -7.237726872051425 1102 82-83 -3.482822766417595 1102 84-85 -2.7183102264533368 1102 86-87 -2.5351037622574424 1102 88-89 -2.6086322891641984 1102 90-91 -7.608434255117089 1102 92-93 -13.128266618222892 1102 94-95 -11.435592763902317 1102 96-97 -11.547497724944954 1102 98-99 -11.178629337987903 1102 100-101 -10.97928757620037 1103 1 0.20894022434185544 1103 2 0.24268801309111154 1103 3 0.22989376313419285 1103 4 0.13745536695282823 1103 5 0.17955864018658474 1103 6 0.2527096296759055 1103 7 0.26128948289026965 1103 8 0.3579776257901486 1103 9 0.36837838302179193 1103 10-11 0.2849421029523924 1103 12-13 0.2583577777644095 1103 14-15 0.4364892694337854 1103 16-17 0.3002648706887143 1103 18-19 0.384318671750286 1103 20-21 0.34322504762150885 1103 22-23 0.32260748896478475 1103 24-25 0.3475469482119351 1103 26-27 0.28632796905128544 1103 28-29 0.4842032219801453 1103 30-31 0.40839827599186407 1103 32-33 0.45628515051896557 1103 34-35 0.49841968650524393 1103 36-37 0.19023529903781622 1103 38-39 0.37207423418734464 1103 40-41 0.49257523152078164 1103 42-43 0.5778061134869148 1103 44-45 0.6379063375719696 1103 46-47 0.7277987360436313 1103 48-49 0.6089078352996466 1103 50-51 0.49373726582862076 1103 52-53 0.4473927670635689 1103 54-55 0.3256187342203063 1103 56-57 0.7751529171871177 1103 58-59 -6.24790774637874 1103 60-61 -8.318949911157002 1103 62-63 -2.414020134892045 1103 64-65 -0.2612114371763852 1103 66-67 -1.7350668082001093 1103 68-69 -3.72621656499979 1103 70-71 -4.350017554112979 1103 72-73 -7.10758991653023 1103 74-75 -7.7422804637389415 1103 76-77 -8.241016662184453 1103 78-79 -11.255939862416405 1103 80-81 -7.760918236685182 1103 82-83 -3.912765752319789 1103 84-85 -3.018862913097667 1103 86-87 -2.6691650040696793 1103 88-89 -2.7873636491570295 1103 90-91 -8.180073863325816 1103 92-93 -13.551682802817483 1103 94-95 -11.720368361217098 1103 96-97 -11.619693022146969 1103 98-99 -11.192360250172758 1103 100-101 -10.982862152512958 1104 1 0.14834894877234106 1104 2 0.16960616298239728 1104 3 0.1812023925744768 1104 4 0.09348496103331172 1104 5 0.13611873894477355 1104 6 0.13615054976775554 1104 7 0.1568450569881179 1104 8 0.12700915173328298 1104 9 0.20534568803606845 1104 10-11 0.2764107708981882 1104 12-13 0.03167747665909104 1104 14-15 0.1377475053212862 1104 16-17 0.25014579258990466 1104 18-19 0.30702989319778595 1104 20-21 0.23103758097018812 1104 22-23 0.26218572896281245 1104 24-25 0.19093432597737348 1104 26-27 0.2197612697257867 1104 28-29 0.25697836309306865 1104 30-31 0.322895238545172 1104 32-33 0.20662626766169012 1104 34-35 0.37027733558782217 1104 36-37 0.23360603265815882 1104 38-39 0.41417843180560254 1104 40-41 0.35500698064476666 1104 42-43 0.366964758539325 1104 44-45 0.3102265972733349 1104 46-47 0.27616403067354867 1104 48-49 0.15781922476956822 1104 50-51 -0.04481435100295528 1104 52-53 0.18318628656246716 1104 54-55 0.062050352474763315 1104 56-57 0.6511448138159963 1104 58-59 -6.0867608022920905 1104 60-61 -8.444089728563267 1104 62-63 -2.498465456679579 1104 64-65 -0.29797238114095137 1104 66-67 -1.3861102292174934 1104 68-69 -3.997873606022118 1104 70-71 -4.712943138096305 1104 72-73 -7.538962461244354 1104 74-75 -8.074039556646207 1104 76-77 -8.815538955457804 1104 78-79 -11.344488031845897 1104 80-81 -8.000611931716136 1104 82-83 -4.442008066040405 1104 84-85 -3.6079246795640607 1104 86-87 -3.3590296858525086 1104 88-89 -3.448908910911463 1104 90-91 -8.437798569165803 1104 92-93 -13.345860601811545 1104 94-95 -11.656905641247592 1104 96-97 -11.321846265098348 1104 98-99 -10.720984886054637 1104 100-101 -10.382932066705582 1105 1 0.10146629845765531 1105 2 0.11466565226423597 1105 3 0.09494141199491679 1105 4 0.07362664649193817 1105 5 0.030319914554908678 1105 6 0.02212297623994175 1105 7 -0.021764543567073247 1105 8 0.06857216184484827 1105 9 0.1599132003918058 1105 10-11 0.048598042339939695 1105 12-13 0.16453237951341038 1105 14-15 0.15807565517461342 1105 16-17 0.31259258009911406 1105 18-19 0.24942212676137387 1105 20-21 0.19880505227784084 1105 22-23 0.2538724051203971 1105 24-25 0.27024322236662357 1105 26-27 0.34295835009393016 1105 28-29 0.14590633849205403 1105 30-31 0.07414624844106754 1105 32-33 0.12218223002616924 1105 34-35 0.23752179170507048 1105 36-37 0.08059299902220829 1105 38-39 0.20285472679869798 1105 40-41 0.25038002683864136 1105 42-43 -0.01462478498338271 1105 44-45 -0.055402025415652645 1105 46-47 -0.28949768268385867 1105 48-49 -0.17007103422470493 1105 50-51 -0.13713862042100544 1105 52-53 -0.0036157547099904264 1105 54-55 -0.15964364624335303 1105 56-57 0.5710393063286361 1105 58-59 -6.382862495438541 1105 60-61 -8.32113354478265 1105 62-63 -2.6833127134588217 1105 64-65 -0.6317250368736396 1105 66-67 -1.8276915022472515 1105 68-69 -3.8620751121041046 1105 70-71 -4.414112225759181 1105 72-73 -6.041978669149415 1105 74-75 -6.382280437579809 1105 76-77 -7.809027138045241 1105 78-79 -10.940884714604223 1105 80-81 -7.5634244124204955 1105 82-83 -4.037564871977768 1105 84-85 -3.250808533492023 1105 86-87 -3.0467574831445567 1105 88-89 -3.0962373938456373 1105 90-91 -8.260942861003755 1105 92-93 -10.886774756529768 1105 94-95 -10.952483579990286 1105 96-97 -11.223717866347531 1105 98-99 -10.831696218021438 1105 100-101 -10.566236759144994 1106 1 0.07058587795831173 1106 2 0.12850547332223528 1106 3 0.10653727737193819 1106 4 0.08091321937921236 1106 5 0.005108623687988256 1106 6 -0.004258761910172382 1106 7 0.058090658460741906 1106 8 0.2899644196591993 1106 9 0.08537348058109018 1106 10-11 0.03217677466654578 1106 12-13 0.09981939042082644 1106 14-15 0.19676055353239974 1106 16-17 0.0382066161316601 1106 18-19 0.20658366643809956 1106 20-21 0.20792044904510476 1106 22-23 0.18715376844016163 1106 24-25 0.24450594828808647 1106 26-27 0.2384348944739898 1106 28-29 0.3665428320330477 1106 30-31 0.12362861274458226 1106 32-33 0.1930656484503004 1106 34-35 0.36552914110871626 1106 36-37 0.21253265636120489 1106 38-39 0.3326042833930529 1106 40-41 0.33491163250562295 1106 42-43 0.2900662492468129 1106 44-45 0.21651558730273024 1106 46-47 0.26042539451539426 1106 48-49 0.3847070654859124 1106 50-51 0.4950716579731065 1106 52-53 0.49995213482276313 1106 54-55 -0.028321076716629534 1106 56-57 0.9768568960689095 1106 58-59 -6.211056721471298 1106 60-61 -8.71312522599015 1106 62-63 -2.177311478385615 1106 64-65 0.012861105892206126 1106 66-67 -1.401691775112262 1106 68-69 -3.647259439599562 1106 70-71 -4.347820838240603 1106 72-73 -5.7703173161698 1106 74-75 -6.363799667382413 1106 76-77 -7.895228246738865 1106 78-79 -11.051663869871597 1106 80-81 -7.336973829710082 1106 82-83 -3.630839377768968 1106 84-85 -2.758256671188324 1106 86-87 -2.648745268844994 1106 88-89 -2.7298021238887955 1106 90-91 -8.02465253263226 1106 92-93 -10.928370962988053 1106 94-95 -10.948828624977109 1106 96-97 -11.275771803861444 1106 98-99 -11.039810990071445 1106 100-101 -10.806676610361757 1107 1 0.15212226609847335 1107 2 0.20070916147859919 1107 3 0.16354794121062355 1107 4 0.10445393786270785 1107 5 0.10638931536459495 1107 6 0.17736894639553924 1107 7 0.2683240403999676 1107 8 0.11839665862345328 1107 9 0.09477246133020856 1107 10-11 0.19295918227852127 1107 12-13 0.20837807835625455 1107 14-15 0.26973935841655816 1107 16-17 0.3391443048477214 1107 18-19 0.2642911988788512 1107 20-21 0.29190278153888727 1107 22-23 0.1509156805665981 1107 24-25 0.3373984408867372 1107 26-27 0.46387050639274463 1107 28-29 0.41214215744214755 1107 30-31 0.32559623090845946 1107 32-33 0.2382755743631293 1107 34-35 0.08759271909172384 1107 36-37 0.1055380103741328 1107 38-39 0.3608297631921502 1107 40-41 0.025157681435558743 1107 42-43 -0.006868817696755514 1107 44-45 0.04657446671593135 1107 46-47 0.09583460459595727 1107 48-49 0.0440800862686217 1107 50-51 0.08585053061093362 1107 52-53 0.01974246666846824 1107 54-55 -0.17466900654739703 1107 56-57 0.6302158579648349 1107 58-59 -6.525593013872577 1107 60-61 -8.536944507801234 1107 62-63 -2.634669011562817 1107 64-65 -0.43977392275581195 1107 66-67 -1.9547918737969567 1107 68-69 -4.085229848920111 1107 70-71 -4.350817281172864 1107 72-73 -5.607423129729373 1107 74-75 -6.034291286547864 1107 76-77 -8.251988148348751 1107 78-79 -11.597868007264257 1107 80-81 -7.655952774520298 1107 82-83 -3.4779176848109294 1107 84-85 -2.5719856270453363 1107 86-87 -2.3592143157275878 1107 88-89 -2.2369622876759294 1107 90-91 -7.359368556375502 1107 92-93 -10.713734512985843 1107 94-95 -11.071786809178722 1107 96-97 -11.742851436518713 1107 98-99 -10.896825888514584 1107 100-101 -10.584339624221801 1108 1 0.030295257086450533 1108 2 -0.002356030601074366 1108 3 -0.09722126764686578 1108 4 -0.033131651443923715 1108 5 -0.19023393501728236 1108 6 0.005872544440030936 1108 7 -0.030108914198081038 1108 8 0.016836022405158246 1108 9 -0.045491632642665536 1108 10-11 0.014510151311490915 1108 12-13 -0.02583930691045566 1108 14-15 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54-55 -0.27472514666759906 2311 56-57 0.846367863611718 2311 58-59 4.146852307899412 2311 60-61 3.8305366952062805 2311 62-63 1.1124655932418754 2311 64-65 0.9953137388741027 2311 66-67 -0.3239719858349872 2311 68-69 -3.3802864042522973 2311 70-71 -1.0990400873830506 2311 72-73 -0.9781771546905311 2311 74-75 1.054785091696413 2311 76-77 3.9493295441798537 2311 78-79 4.593448699135891 2311 80-81 2.512182560365467 2311 82-83 1.3907576851602492 2311 84-85 1.1998969094846679 2311 86-87 0.9587775115165478 2311 88-89 1.0149727731699265 2311 90-91 2.417884487647349 2311 92-93 4.183212035391541 2311 94-95 8.073116982744715 2311 96-97 8.689737167988413 2311 98-99 9.143752865088247 2311 100-101 8.855747016678654 2312 1 -0.34788880974714687 2312 2 -0.40359296277998524 2312 3 -0.4423641679670851 2312 4 -0.23567907861283288 2312 5 -0.3107319040062606 2312 6 -0.27945519221379556 2312 7 -0.3918034470134373 2312 8 -0.36981775557064367 2312 9 -0.2852694851123232 2312 10-11 -0.522992117671329 2312 12-13 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2312 78-79 4.930590713502156 2312 80-81 2.5210540800990415 2312 82-83 1.212054776206525 2312 84-85 1.047045599750767 2312 86-87 1.051183865352744 2312 88-89 0.9842786146244791 2312 90-91 2.525398124291037 2312 92-93 4.247456388517104 2312 94-95 8.057654383397452 2312 96-97 8.837678821805449 2312 98-99 9.19381240803808 2312 100-101 8.24082638055233 2313 1 -0.28015082928063606 2313 2 -0.19309681678883805 2313 3 -0.25327787194736473 2313 4 -0.25455637241251594 2313 5 -0.27342587325421164 2313 6 -0.2736391156836788 2313 7 -0.2681849705146604 2313 8 -0.3472820910894825 2313 9 -0.26879165456067255 2313 10-11 -0.3856385356816574 2313 12-13 -0.34073775208624824 2313 14-15 -0.4827188505206337 2313 16-17 -0.36171720144359654 2313 18-19 -0.33049564554840316 2313 20-21 -0.2571631897180495 2313 22-23 -0.2834227938027638 2313 24-25 -0.3360933942939113 2313 26-27 -0.28228882543373146 2313 28-29 -0.4647373827655059 2313 30-31 -0.2777860349346781 2313 32-33 -0.29606380653687125 2313 34-35 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8.646973757379252 2314 1 -0.38273430613110193 2314 2 -0.2878796162974062 2314 3 -0.31941873732934667 2314 4 -0.17585001878377682 2314 5 -0.45471612491355984 2314 6 -0.3419141008265498 2314 7 -0.40955492630337886 2314 8 -0.516431825261634 2314 9 -0.5022320097671553 2314 10-11 -0.5460032945286528 2314 12-13 -0.5437781672687905 2314 14-15 -0.6107795139383114 2314 16-17 -0.5781406155213773 2314 18-19 -0.5607777814876655 2314 20-21 -0.559834398391331 2314 22-23 -0.4995755996416662 2314 24-25 -0.41691449339717934 2314 26-27 -0.5974905644022854 2314 28-29 -0.41450070058677113 2314 30-31 -0.5325503193643684 2314 32-33 -0.6529002551290972 2314 34-35 -0.6900621167996874 2314 36-37 -0.7242182678581983 2314 38-39 -0.5359349379992651 2314 40-41 -0.5654653304501522 2314 42-43 -0.6572593077098929 2314 44-45 -0.5556730881803915 2314 46-47 -0.5249758460403271 2314 48-49 -0.5481152526611197 2314 50-51 -0.6804114689602088 2314 52-53 -0.605865597255999 2314 54-55 -0.6258154451756894 2314 56-57 0.6059768128622167 2314 58-59 3.8193017301513414 2314 60-61 3.514513649426153 2314 62-63 1.2317135375120394 2314 64-65 1.4329632819842928 2314 66-67 1.7672059517930307 2314 68-69 -0.48864803209155916 2314 70-71 1.9895077904657068 2314 72-73 7.7237291480847325 2314 74-75 9.326090369370803 2314 76-77 8.371665028824019 2314 78-79 6.162674868663231 2314 80-81 3.525985046568465 2314 82-83 1.9165255191388049 2314 84-85 1.532252700342486 2314 86-87 1.636325219724867 2314 88-89 1.6795448868138294 2314 90-91 2.7788498008196356 2314 92-93 4.710309774447417 2314 94-95 8.325570228236373 2314 96-97 8.848855679136152 2314 98-99 9.29900635938588 2314 100-101 8.49263440159112 2315 1 -0.6559447342865852 2315 2 -0.5126203212578915 2315 3 -0.45782928892671393 2315 4 -0.40637907458755507 2315 5 -0.644830040302196 2315 6 -0.5931290641294211 2315 7 -0.7048372996077319 2315 8 -0.7430845376286328 2315 9 -0.8879704471539398 2315 10-11 -0.7474624580757308 2315 12-13 -0.7114524953826802 2315 14-15 -0.8400828723625509 2315 16-17 -0.9713325273946083 2315 18-19 -0.7980820523084162 2315 20-21 -0.810590479916641 2315 22-23 -0.820419834771613 2315 24-25 -1.0550479716405405 2315 26-27 -0.8743787555761955 2315 28-29 -0.8243069505029155 2315 30-31 -0.7856122147339519 2315 32-33 -0.7015075086814022 2315 34-35 -0.870618678674937 2315 36-37 -0.8936469466275412 2315 38-39 -0.9776569522487506 2315 40-41 -0.8236614688660779 2315 42-43 -0.8491483710275247 2315 44-45 -0.8699863085375696 2315 46-47 -0.8901363875744934 2315 48-49 -0.6886243162471075 2315 50-51 -0.7602924548851391 2315 52-53 -0.7628977592332191 2315 54-55 -0.6905687626762607 2315 56-57 0.5096632973179354 2315 58-59 3.9131054843946593 2315 60-61 3.4377153561443414 2315 62-63 0.9107391517024865 2315 64-65 1.0739406196649277 2315 66-67 2.0445053922792837 2315 68-69 6.246152515346353 2315 70-71 4.595489354689027 2315 72-73 8.65078505920658 2315 74-75 9.630620149724756 2315 76-77 8.483697257889219 2315 78-79 5.9072340335520686 2315 80-81 3.281183023195016 2315 82-83 1.6392495594452647 2315 84-85 1.2790411987793355 2315 86-87 1.208260976276005 2315 88-89 1.1589817503166273 2315 90-91 2.2902688457551648 2315 92-93 4.295612810849352 2315 94-95 7.994340773929995 2315 96-97 8.559266391744666 2315 98-99 8.795533885583732 2315 100-101 8.087326400678725 2316 1 -0.5936552862828535 2316 2 -0.5225047739994864 2316 3 -0.581497384023379 2316 4 -0.409995406775316 2316 5 -0.5652763893199761 2316 6 -0.5740871022303153 2316 7 -0.6076998167559609 2316 8 -0.6164549252847351 2316 9 -0.8357252663373345 2316 10-11 -0.7013687191248508 2316 12-13 -0.9282513978958633 2316 14-15 -1.107252318103427 2316 16-17 -0.9719604708796936 2316 18-19 -1.1388736288142738 2316 20-21 -0.8520319213580834 2316 22-23 -0.8199729200389854 2316 24-25 -2.528914066935208 2316 26-27 -1.7328566997684263 2316 28-29 -1.5501777978023341 2316 30-31 -1.0100899892117283 2316 32-33 -0.8141561856158006 2316 34-35 -1.0435280087271224 2316 36-37 -1.0762448506540068 2316 38-39 -1.4788689963179351 2316 40-41 -1.4160526041143555 2316 42-43 -1.775069425520016 2316 44-45 -1.4404128575355486 2316 46-47 -1.3714939617892057 2316 48-49 -1.7001578277806217 2316 50-51 -1.5790112521753699 2316 52-53 -1.1985144591356303 2316 54-55 -1.0565509143726999 2316 56-57 0.04231847997311888 2316 58-59 3.5342207912242465 2316 60-61 3.266206170349445 2316 62-63 0.7814407795469727 2316 64-65 0.4918949947621627 2316 66-67 5.2573973265997935 2316 68-69 7.991441524921072 2316 70-71 4.939718356060885 2316 72-73 8.517401518680181 2316 74-75 9.478861483680376 2316 76-77 7.9565931522136815 2316 78-79 5.511725102328853 2316 80-81 2.576805097388519 2316 82-83 1.0820105307776622 2316 84-85 0.683948775115482 2316 86-87 0.7557612237762577 2316 88-89 0.7947695789615992 2316 90-91 2.1967469879475914 2316 92-93 3.7383674178896698 2316 94-95 7.487687967277186 2316 96-97 8.153180714230418 2316 98-99 8.554368230132363 2316 100-101 7.696341066836249 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 13.0 4 50.0 5 157.0 6 301.0 7 674.0 8 1322.0 9 2332.0 10 3998.0 11 5806.0 12 8057.0 13 10829.0 14 13599.0 15 18739.0 16 24719.0 17 31351.0 18 31436.0 19 27932.0 20 28541.0 21 27835.0 22 26653.0 23 25919.0 24 26808.0 25 27894.0 26 35952.0 27 51654.0 28 70996.0 29 91652.0 30 106465.0 31 111607.0 32 113720.0 33 117194.0 34 117879.0 35 110754.0 36 88115.0 37 51546.0 38 14835.0 39 649.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.75486566110371 18.780128013852057 14.125721986638256 21.339284338405974 2 16.36543428446571 21.845884060082135 37.97124752785886 23.817434127593298 3 20.112526839626142 26.847609750085088 29.47190540686282 23.56795800342595 4 13.006874693187843 17.162363068114576 35.51983170762281 34.310930531074774 5 13.425071073740803 39.01701355043555 31.764163418098594 15.793751957725052 6 31.02747990355668 35.6236637677181 17.28591746855193 16.062938860173293 7 28.600670452404813 34.58936365845443 19.105932740472454 17.704033148668305 8 28.04860541642828 32.483933699769395 19.057289656993156 20.41017122680917 9 28.57924174592137 16.22755325488746 19.399141871342326 35.794063127848844 10-11 26.066655882284973 26.81578525354852 25.105690442026596 22.011868422139916 12-13 27.925736226628878 24.4153499566874 26.363156350425175 21.295757466258543 14-15 24.10371239244041 25.019126567984664 26.59827204369786 24.278888995877065 16-17 22.04859834550778 28.845735022139497 26.65885149136094 22.446815140991784 18-19 21.67994949479034 26.34635915352652 30.761363234490325 21.212328117192815 20-21 23.158864446624662 25.088183328886526 30.249630424841612 21.503321799647196 22-23 22.367893074956356 25.416336657583976 30.20778842443685 22.00798184302282 24-25 22.285224486708948 25.722220938367258 30.551523083828958 21.44103149109483 26-27 22.09871511525307 25.77416616946361 30.83212183855887 21.294996876724454 28-29 21.386638393172905 26.20726674542721 31.119249389162277 21.28684547223761 30-31 21.401655684679646 26.196144813055266 30.81019015406531 21.59200934819978 32-33 21.221749100393527 26.365603852420072 30.40367006527727 22.008976981909132 34-35 22.07951819884802 26.04597419422609 30.23183178977223 21.642675817153663 36-37 22.199436948848366 25.706668422553147 30.393694499568852 21.700200129029636 38-39 21.875569141797214 25.797288853646847 30.65874047694433 21.668401527611607 40-41 21.60506626336808 25.947892944679772 30.857945825082076 21.589094966870068 42-43 21.491746687326803 26.31662823246574 29.981268580811292 22.210356499396166 44-45 21.5150888941816 26.43505611051179 29.70361601075977 22.346238984546844 46-47 21.684713794099878 26.694162047617798 29.04405390495705 22.577070253325278 48-49 22.034548366171492 26.62325062031263 28.88092939714439 22.46127161637149 50-51 21.701375754773814 26.956632903234844 28.866578405413357 22.475412936577985 52-53 22.039568383117768 27.00085104594597 28.480305115731742 22.479275455204515 54-55 21.027248925914357 26.737518263308225 28.88646000703216 23.34877280374526 56-57 21.71200492067895 27.14954452509592 28.304143687191825 22.83430686703331 58-59 20.43341315861146 26.361851231458967 29.989867787325135 23.21486782260443 60-61 21.817241194012865 26.4320627808203 28.965921020328818 22.78477500483802 62-63 21.7286641965228 26.526256891694395 28.64875520575467 23.096323706028134 64-65 20.64080536347953 26.658414800061713 29.28450706200822 23.41627277445054 66-67 20.86515485794701 26.791513218472236 29.035683074837266 23.307648848743494 68-69 21.61283637784409 26.293830125725933 28.963266557248 23.13006693918198 70-71 20.77478247425337 26.76167939324704 29.057903793351258 23.405634339148335 72-73 20.69221255524405 26.528666976070213 29.08873758001001 23.690382888675725 74-75 21.084844012553678 26.86600816166861 28.391191087955757 23.657956737821955 76-77 19.715816534445906 27.026107448442048 29.166352445420213 24.091723571691833 78-79 20.476233172819125 27.29529164183355 28.332181780143 23.896293405204318 80-81 20.812758077002666 27.946809132700206 27.566484207341862 23.67394858295527 82-83 21.23912149090941 27.566312730328523 26.93265872425354 24.26190705450853 84-85 20.996470563519352 27.230232704710815 26.832295744367958 24.941000987401875 86-87 21.3086265426425 27.092942842786588 27.017589932402974 24.58084068216794 88-89 21.18970679426789 27.08505413726492 26.92847046251358 24.796768605953613 90-91 20.73027710693704 27.37272332329002 27.191438411205837 24.7055611585671 92-93 20.65992993887277 27.20051183136503 27.38932681477927 24.750231414982924 94-95 19.416032919615503 27.163045305613743 28.131701161280436 25.289220613490315 96-97 19.162902257510765 26.96552156998468 28.35698478007328 25.514591392431278 98-99 19.47766150920894 27.090942015914482 27.54864782083087 25.882748654045717 100-101 19.247649475895702 27.220271459870233 27.788004241208913 25.744074823025155 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2415.0 1 1679.0 2 966.5 3 1146.0 4 1378.5 5 1525.0 6 1948.0 7 3047.5 8 4066.5 9 4461.0 10 4436.5 11 4416.5 12 4718.0 13 4953.0 14 5000.0 15 4928.0 16 4811.5 17 4758.0 18 4814.5 19 4901.0 20 5114.5 21 5457.5 22 5897.5 23 6429.5 24 7030.5 25 7792.0 26 8777.0 27 10014.5 28 11295.0 29 12602.0 30 14137.0 31 15888.0 32 17702.0 33 19531.5 34 21408.5 35 23121.0 36 24875.0 37 26811.5 38 28342.0 39 29326.5 40 31122.5 41 34137.0 42 37265.0 43 40354.0 44 43693.5 45 47055.5 46 50841.0 47 56097.5 48 62003.5 49 63085.0 50 60085.5 51 57788.0 52 55382.5 53 53584.0 54 51464.5 55 47590.0 56 43300.5 57 39078.0 58 34615.0 59 30033.0 60 25568.0 61 21059.0 62 16849.0 63 13267.5 64 10390.0 65 8012.0 66 6036.0 67 4581.5 68 3332.0 69 2363.0 70 1759.0 71 1443.0 72 1238.0 73 980.0 74 718.5 75 471.5 76 275.5 77 153.5 78 85.5 79 47.0 80 27.0 81 16.0 82 11.5 83 6.0 84 4.5 85 4.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023319474702571657 2 0.005532247752261744 3 0.00455184941641789 4 0.0 5 0.06666708683738203 6 1.4005690512055048E-4 7 0.0 8 1.4005690512055048E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.4509958396096333E-4 16-17 0.0 18-19 0.001365554824925367 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.1008535768082573E-4 28-29 0.002380967387049358 30-31 0.008578485438633716 32-33 3.851564890815138E-4 34-35 0.0011204552409644038 36-37 0.0288517224548334 38-39 0.02822146638179092 40-41 0.03011223460091835 42-43 0.03102260448420193 44-45 0.031582832104684135 46-47 0.03350861455009171 48-49 0.03396379949173349 50-51 0.028781694002273123 52-53 0.02352956006025248 54-55 0.01855753992847294 56-57 0.15206678473463767 58-59 0.751335267519193 60-61 0.3061293803672432 62-63 2.8011381024110096E-4 64-65 0.14362835620112452 66-67 1.3965424151832888 68-69 2.6766275137588402 70-71 2.1649296109009093 72-73 1.846615279788178 74-75 1.5426217672240232 76-77 1.1043136826492603 78-79 0.42006567268281103 80-81 1.0504267884041287E-4 82-83 2.4509958396096333E-4 84-85 7.002845256027524E-5 86-87 0.003046237686371973 88-89 3.501422628013762E-5 90-91 0.2214299669955903 92-93 0.17657674313073402 94-95 1.2782993730352643 96-97 1.2580261360190645 98-99 1.0021071561375388 100-101 0.40301374448438404 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1427991.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.906860003100576 #Duplication Level Percentage of deduplicated Percentage of total 1 73.17067578845645 30.663532665991056 2 13.449481226891496 11.272510537793428 3 5.1297554140650234 6.449158259621106 4 2.492441059556861 4.178015141953162 5 1.438327964608723 3.0137904325701186 6 0.8512679355848115 2.1404379721008686 7 0.6173408201440729 1.8109570726783868 8 0.41900861979209103 1.404746845577565 9 0.32109260505710163 1.2110384563342942 >10 1.779181994876413 13.951794377537569 >50 0.18421197785527438 5.373597883610437 >100 0.1344861555031108 10.492019126079187 >500 0.008206492668699397 2.2500729494491134 >1k 0.003852027171022166 2.931994056684136 >5k 3.349588844367101E-4 1.0040719802495093 >10k+ 3.349588844367101E-4 1.8522622417702221 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14841 1.0392922644470448 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11550 0.808828627071179 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9087 0.636348548415221 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5219 0.36547849391207643 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3764 0.26358709543687603 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3321 0.2325644909526741 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3306 0.23151406416426992 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2875 0.20133180111079133 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2592 0.1815137490362334 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2133 0.1493706893110671 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1905 0.13340420212732432 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1854 0.1298327510467503 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1630 0.11414637767324864 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1555 0.108894243731228 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1535 0.10749367468002251 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1489 0.10427236586224983 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 1442 0.10098102859191689 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 7.002845256027524E-5 0.0 16-17 0.0 0.0 0.0 7.002845256027524E-5 0.0 18-19 0.0 0.0 0.0 1.750711314006881E-4 0.0 20-21 0.0 0.0 0.0 8.753556570034406E-4 0.0 22-23 0.0 0.0 0.0 0.0025560385184500464 0.0 24-25 0.0 0.0 0.0 0.0057423331099425694 0.0 26-27 0.0 0.0 0.0 0.010364210978920735 0.0 28-29 0.0 0.0 0.0 0.025945541673581977 0.0 30-31 0.0 0.0 0.0 0.06708725755274368 0.0 32-33 0.0 0.0 0.0 0.13984681976286967 0.0 34-35 0.0 0.0 0.0 0.2445743705667613 0.0 36-37 0.0 0.0 0.0 0.40956140479876973 0.0 38-39 0.0 0.0 0.0 0.6628193034830052 0.0 40-41 0.0 0.0 0.0 0.9435633697971486 0.0 42-43 0.0 0.0 0.0 1.2093213472633932 0.0 44-45 0.0 0.0 0.0 1.5065571141554814 0.0 46-47 0.0 0.0 0.0 1.8549136514165707 0.0 48-49 0.0 0.0 0.0 2.199488652239405 0.0 50-51 0.0 0.0 0.0 2.543293340084076 0.0 52-53 0.0 0.0 0.0 2.8549549682035815 0.0 54-55 0.0 0.0 0.0 3.1783813763532125 0.0 56-57 0.0 0.0 0.0 3.4562542761123844 0.0 58-59 0.0 0.0 0.0 3.655940408588009 0.0 60-61 0.0 0.0 0.0 3.8102481038045757 0.0 62-63 0.0 0.0 0.0 3.939030428062922 0.0 64-65 3.501422628013762E-5 0.0 0.0 4.061160049328042 0.0 66-67 7.002845256027524E-5 0.0 0.0 4.158639655291945 0.0 68-69 7.002845256027524E-5 0.0 0.0 4.269634752599981 0.0 70-71 7.002845256027524E-5 0.0 0.0 4.488683752208523 0.0 72-73 7.002845256027524E-5 0.0 0.0 4.749014524601345 0.0 74-75 7.002845256027524E-5 0.0 0.0 5.047055618697877 0.0 76-77 7.002845256027524E-5 0.0 0.0 5.463339754942433 0.0 78-79 7.002845256027524E-5 0.0 0.0 6.032881159615152 0.0 80-81 7.002845256027524E-5 0.0 0.0 6.70662490169756 0.0 82-83 7.002845256027524E-5 0.0 0.0 7.339156899448246 0.0 84-85 7.002845256027524E-5 0.0 0.0 7.847598479262125 0.0 86-87 7.002845256027524E-5 0.0 0.0 8.29637581749465 0.0 88-89 7.002845256027524E-5 0.0 0.0 8.72925669699599 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 800 0.0 27.103647 2 GTACATG 13045 0.0 25.408419 1 TACATGG 13105 0.0 25.142012 2 GTATCAA 18580 0.0 24.005558 1 ATGGGAG 2475 0.0 23.805618 5 ACATGGG 13160 0.0 23.675013 3 AGTACTT 9660 0.0 22.361921 12-13 GTGTACG 170 3.3596807E-6 22.187452 1 TACGCGG 55 0.0024736957 22.161871 64-65 GAGTACT 9535 0.0 22.086847 12-13 TGCACCG 470 0.0 22.06325 5 CATGGGA 8230 0.0 21.706211 4 AGGTAAG 290 1.1823431E-10 21.130278 2 GGTATCA 13840 0.0 21.121365 1 GTACTTT 10120 0.0 20.996677 14-15 CATGGGC 1450 0.0 20.80447 4 TACTTTT 10480 0.0 20.47772 14-15 ATCAACG 21910 0.0 20.329847 3 TCAACGC 21985 0.0 20.281933 4 TATCAAC 22060 0.0 20.235052 2 >>END_MODULE