FastQCFastQC Report
Fri 27 May 2016
SRR939744_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939744_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1486097
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141490.952091283408822No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102220.6878420453039068No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76040.5116758865672967No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43760.2944626091029051No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA34970.2353143839197576No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29800.20052526853899846No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA28500.19177752192488107No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25200.16957170359673696No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21710.1460873684557603No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19380.13040871490891914No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16240.10927954231789713No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14910.10032992462806936No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT14880.10012805355235897No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG9200.027.608162
GTACATG130700.026.0174661
GTATCAA199350.025.3626421
TACATGG132000.025.2997342
CCTTACG1151.3703085E-424.5405862
ACATGGG133300.024.1716673
AGTACTT102350.023.69119612-13
GTACGTG1652.648534E-622.8038581
GAGTACT103350.022.55170412-13
CATGGGG46600.022.4092794
TACGTGT1503.272744E-521.9501918
ACTTGAC6300.021.6515488
GTAAGGT4350.021.6272624
TATCAAC234650.021.5285742
TATAACG1100.003046352221.3800562
GTACTTT110400.021.3747814-15
ATGGGAT30400.021.3533655
CATGGGA85400.021.31644
TCAACGC239250.021.0374284
ATCAACG239600.020.9470943
Produced by FastQC (version 0.11.5)