FastQCFastQC Report
Fri 27 May 2016
SRR939744_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939744_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1486097
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150471.012518025404802No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121050.8145497904914687No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93880.6317218862564153No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55020.37023155285287573No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA37990.255636072207938No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34370.23127696240554957No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA32250.21701140638868124No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29730.20005423602900752No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26900.18101106455365967No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT21780.14655840096575123No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18900.12717877769755273No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18560.12489090550616816No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15660.1053767015208294No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15510.10436734614227738No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA15260.10268508717802403No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15120.10174302215804217No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG14900.10026263426949923No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATC601.50081E-647.4621121
CGTATCA953.5146273E-529.9710252
GTACATG138450.025.9850351
TACATGG139450.025.5561492
ATGGGCG2059.713403E-1025.4554795
TAAGGTG5300.025.0625155
ACATGGG139900.024.143753
GTAAGGT3950.024.020134
ATGGGAG24900.022.8625345
CATGGGG45600.022.4714664
GGTATCA152200.022.2030411
GTATCAA197250.022.1610181
GAGTACT103550.021.87588912-13
CATGGGA87950.021.6836624
AGTACTT101950.021.59102212-13
AGGTAAG3750.021.5125392
ATGGGAT30650.021.3594995
ACCGTGC1353.8293414E-421.0843378
GTACTTT109750.020.74813814-15
GTATAGC3001.6916601E-1020.5669161
Produced by FastQC (version 0.11.5)