##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939744_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1486097 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.38584224313756 31.0 31.0 34.0 30.0 34.0 2 31.587897694430445 31.0 31.0 34.0 30.0 34.0 3 31.612988923334076 31.0 31.0 34.0 30.0 34.0 4 35.43023773010779 37.0 35.0 37.0 33.0 37.0 5 35.1886094918434 37.0 35.0 37.0 32.0 37.0 6 35.232918847154664 37.0 35.0 37.0 32.0 37.0 7 35.13624413480412 37.0 35.0 37.0 32.0 37.0 8 35.108497628351316 37.0 35.0 37.0 32.0 37.0 9 36.647086293828735 39.0 35.0 39.0 32.0 39.0 10-11 36.520767486913705 38.0 35.0 39.0 32.0 39.0 12-13 36.416028025088536 38.0 35.0 39.0 32.0 39.0 14-15 37.34047811145571 39.0 36.0 41.0 32.0 41.0 16-17 37.29659975089109 39.0 36.0 41.0 32.0 41.0 18-19 37.35677011662092 39.0 36.0 41.0 32.0 41.0 20-21 37.3153858731967 39.0 36.0 41.0 31.0 41.0 22-23 37.17625363620275 39.0 36.0 40.0 31.0 41.0 24-25 36.9895383679531 39.0 36.0 40.0 31.0 41.0 26-27 36.65096962042182 38.5 35.0 40.0 30.0 41.0 28-29 36.39063668118568 38.0 35.0 40.0 30.0 41.0 30-31 36.05030492625986 38.0 35.0 40.0 30.0 41.0 32-33 35.90708715514533 38.0 34.5 40.0 30.0 41.0 34-35 35.67111669022951 38.0 34.0 40.0 29.0 41.0 36-37 35.58363451376323 38.0 34.0 40.0 28.5 41.0 38-39 35.41112188504519 38.0 34.0 40.0 27.0 41.0 40-41 35.244866923222375 38.0 34.0 40.0 27.0 41.0 42-43 34.92240445946665 38.0 33.5 40.0 26.0 41.0 44-45 34.542131839307935 38.0 33.0 40.0 24.5 41.0 46-47 34.053021774487135 38.0 33.0 40.0 23.5 41.0 48-49 33.58762113105672 37.0 33.0 40.0 21.5 41.0 50-51 33.29685444489828 37.0 32.5 40.0 20.0 41.0 52-53 32.998390414623 37.0 32.0 40.0 19.0 41.0 54-55 32.701122470471304 36.0 31.5 40.0 17.5 41.0 56-57 32.31342334988901 36.0 31.0 39.0 14.5 41.0 58-59 31.928649677645538 35.0 31.0 39.0 12.5 40.5 60-61 31.574373005261435 35.0 30.0 39.0 8.5 40.0 62-63 31.257466033509253 35.0 30.0 38.5 7.5 40.0 64-65 30.790985043372004 35.0 29.5 38.0 6.5 40.0 66-67 29.895590597383617 34.0 28.5 37.5 2.0 40.0 68-69 28.06392853225597 33.0 24.0 36.0 2.0 39.0 70-71 27.415887388239128 33.0 22.5 36.0 2.0 39.0 72-73 27.934521434334368 33.0 25.5 35.0 2.0 38.5 74-75 28.023254538566462 33.0 26.0 35.0 2.0 37.5 76-77 27.546924258645298 32.5 26.0 35.0 2.0 36.5 78-79 28.03791004221124 33.0 26.5 35.0 2.0 37.0 80-81 27.850800788912164 33.0 26.0 35.0 2.0 36.0 82-83 27.42226247681006 33.0 26.0 35.0 2.0 36.0 84-85 27.093741862072264 33.0 25.5 35.0 2.0 35.5 86-87 26.715204996712863 33.0 25.0 35.0 2.0 35.0 88-89 26.26016471333971 32.5 24.5 35.0 2.0 35.0 90-91 25.765607493992654 32.0 23.0 34.5 2.0 35.0 92-93 25.29116235346683 32.0 20.0 34.0 2.0 35.0 94-95 24.51598516113013 31.0 17.5 34.0 2.0 35.0 96-97 23.989054550274982 31.0 6.5 34.0 2.0 35.0 98-99 23.33186292684798 31.0 2.0 34.0 2.0 35.0 100-101 21.961866553798306 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2611489973269272 1101 2 0.20902876777349988 1101 3 0.25186756239098074 1101 4 0.22056360426029187 1101 5 0.27090109287101427 1101 6 0.2512972868983425 1101 7 0.20225783082633342 1101 8 0.10171867005142587 1101 9 0.44081780772343393 1101 10-11 0.4459797046171303 1101 12-13 0.43439627278983295 1101 14-15 0.33607573911443467 1101 16-17 0.3976299196839861 1101 18-19 0.41058518067100636 1101 20-21 0.29102729464089094 1101 22-23 0.43310159872331155 1101 24-25 0.4191398300991551 1101 26-27 0.3401142503224861 1101 28-29 0.22953188987730044 1101 30-31 0.3805205579634361 1101 32-33 0.1693805526899297 1101 34-35 0.14886381316424746 1101 36-37 0.5648076251869014 1101 38-39 0.6141330698704763 1101 40-41 0.47903235046390336 1101 42-43 0.2179031957326245 1101 44-45 0.28637305359183785 1101 46-47 0.2667754158427229 1101 48-49 0.298424995619186 1101 50-51 0.14974948265891896 1101 52-53 0.06162329258058463 1101 54-55 -0.18788245559453287 1101 56-57 -0.12997418335857702 1101 58-59 -0.062400050745907265 1101 60-61 -0.29440328422516515 1101 62-63 -0.042997047084678996 1101 64-65 -0.21506619329655408 1101 66-67 -0.3529528251516467 1101 68-69 -8.618167521911534 1101 70-71 -6.279730635915591 1101 72-73 -1.8011611785066535 1101 74-75 -0.8399755285729817 1101 76-77 -0.6241046529582945 1101 78-79 -0.7807815849968129 1101 80-81 -0.7977014399995177 1101 82-83 -0.7110205574244439 1101 84-85 -0.42681903712323077 1101 86-87 -0.6470387359112024 1101 88-89 -0.40922465969640953 1101 90-91 -0.6175079779153911 1101 92-93 -0.8039867736750992 1101 94-95 -0.3194560137935518 1101 96-97 -0.6355431634316986 1101 98-99 -0.5261436974538896 1101 100-101 -0.40929542778350836 1102 1 0.16003371582558756 1102 2 0.1415202780505318 1102 3 0.22898105658222434 1102 4 0.16437236207977435 1102 5 0.2244149445242769 1102 6 0.2779782520457985 1102 7 0.21538919811855806 1102 8 0.16580088631594947 1102 9 0.27906624382709566 1102 10-11 0.22356951694957417 1102 12-13 0.18757053552442216 1102 14-15 0.20130987673730516 1102 16-17 0.32764421816477096 1102 18-19 0.17979072133231 1102 20-21 0.09715240634778155 1102 22-23 0.331766478079885 1102 24-25 0.10858487031362785 1102 26-27 0.25891139062633073 1102 28-29 0.1271985565439664 1102 30-31 0.0016492442905047255 1102 32-33 -0.017967079124190377 1102 34-35 0.11501930914280223 1102 36-37 -0.06083580644848041 1102 38-39 0.2029561261707471 1102 40-41 0.16408060783655998 1102 42-43 0.04523921003110587 1102 44-45 0.3547421331271394 1102 46-47 0.15781563031993073 1102 48-49 0.35334188569961356 1102 50-51 0.4955376864122627 1102 52-53 0.14902275638755924 1102 54-55 -0.11782972994662799 1102 56-57 -0.10420832098145638 1102 58-59 -0.13354035458862157 1102 60-61 -0.08513965598566742 1102 62-63 -0.18903547425179212 1102 64-65 -0.26085350339485913 1102 66-67 0.2246691587625591 1102 68-69 -4.241592901714931 1102 70-71 0.10257588776626747 1102 72-73 0.5046717079991581 1102 74-75 0.13362679493014795 1102 76-77 -0.14980527851682623 1102 78-79 -0.36703985130601424 1102 80-81 -0.2295432630558203 1102 82-83 -0.2357989309722548 1102 84-85 -0.3887341398935611 1102 86-87 -0.5383765375197811 1102 88-89 -0.5427653210011485 1102 90-91 -0.7004927857795558 1102 92-93 -0.7611842714409605 1102 94-95 -0.3773916706300149 1102 96-97 -0.7124091151743279 1102 98-99 -0.5986164409748902 1102 100-101 -0.6667547664787712 1103 1 0.1861862765944231 1103 2 0.1810658290335816 1103 3 0.14667244575701588 1103 4 0.20439966268627785 1103 5 0.22825579093074566 1103 6 0.1624784510579218 1103 7 0.15752532592117063 1103 8 0.1338712752400042 1103 9 0.18809271507028313 1103 10-11 0.36175341292321406 1103 12-13 0.25655585421559834 1103 14-15 0.35525079906646795 1103 16-17 0.3572556009571528 1103 18-19 0.27434537251753 1103 20-21 0.2729254851793712 1103 22-23 0.26996948623148853 1103 24-25 0.20924708974597905 1103 26-27 0.2060793691365319 1103 28-29 0.2880880267859567 1103 30-31 0.33298164364034477 1103 32-33 0.48970080557852924 1103 34-35 0.2665374560681002 1103 36-37 0.31773764480757194 1103 38-39 0.46397806660036167 1103 40-41 0.5432763007582153 1103 42-43 0.38421494633214337 1103 44-45 0.40534841875425087 1103 46-47 0.3914869920363131 1103 48-49 0.4641261074569911 1103 50-51 0.6671575925346502 1103 52-53 0.5054100813314051 1103 54-55 0.38957492395637416 1103 56-57 0.48367547872992844 1103 58-59 0.47987420257870994 1103 60-61 0.37103011451038626 1103 62-63 0.41950218988938204 1103 64-65 0.413132411456008 1103 66-67 0.4978444279549592 1103 68-69 -3.786343671900635 1103 70-71 0.6824882435263113 1103 72-73 0.7492576137322828 1103 74-75 0.4922411707947951 1103 76-77 0.4416666572662571 1103 78-79 0.3509812229386604 1103 80-81 0.44612066595208333 1103 82-83 0.4076526494645911 1103 84-85 0.3453885059332329 1103 86-87 0.1866682621267337 1103 88-89 0.00706354610258586 1103 90-91 -0.18670462060124393 1103 92-93 0.003405421659543606 1103 94-95 -0.04094391438217215 1103 96-97 -0.5408459757273185 1103 98-99 -0.2534776848094573 1103 100-101 -0.1946738886511774 1104 1 0.23448277128595052 1104 2 0.296800964292558 1104 3 0.2788757139662188 1104 4 0.22955941831624926 1104 5 0.39785460686087504 1104 6 0.25778241578135663 1104 7 0.15453013750181555 1104 8 0.3346219527128085 1104 9 0.2834593587258567 1104 10-11 0.21402068280615794 1104 12-13 0.22160975593590138 1104 14-15 0.34709843133738616 1104 16-17 0.3301681439624602 1104 18-19 0.39439130536807454 1104 20-21 0.3391433995098083 1104 22-23 0.33573676065325486 1104 24-25 0.2518417468209009 1104 26-27 0.44801334703982576 1104 28-29 0.2557511011573155 1104 30-31 0.210831860012334 1104 32-33 0.29147198252029227 1104 34-35 0.32330598104262975 1104 36-37 0.42654589352795114 1104 38-39 0.4207827724481348 1104 40-41 0.345134795936481 1104 42-43 0.30122198841823433 1104 44-45 0.15281500115738567 1104 46-47 0.1253662948909664 1104 48-49 0.1617620742708752 1104 50-51 0.24984796250028296 1104 52-53 0.19106810421310882 1104 54-55 0.3501014615678386 1104 56-57 0.31460986599149265 1104 58-59 0.13942061459888677 1104 60-61 0.1338124020493403 1104 62-63 0.2887165462178132 1104 64-65 0.33112437731371 1104 66-67 0.39192027465006873 1104 68-69 -3.7936088085385435 1104 70-71 0.7523482360826499 1104 72-73 1.1903759442076023 1104 74-75 0.8514908737182729 1104 76-77 0.6821568583878168 1104 78-79 0.5441164101563061 1104 80-81 0.42813332536730186 1104 82-83 0.24999244103336693 1104 84-85 0.44221643324469184 1104 86-87 0.4134278866928973 1104 88-89 0.24412585803877107 1104 90-91 0.4202128857109564 1104 92-93 0.2877021445888346 1104 94-95 0.5912941514410797 1104 96-97 0.2061118266542259 1104 98-99 0.1824800082081488 1104 100-101 0.07411347285539804 1105 1 0.1178734871228464 1105 2 0.1718110806986708 1105 3 0.16816007939778288 1105 4 0.10074047500859251 1105 5 0.22181775953767868 1105 6 0.1018278991432382 1105 7 0.2634619124589861 1105 8 0.2709210510038105 1105 9 0.26756100500234226 1105 10-11 0.20641082706610803 1105 12-13 0.13640137483064763 1105 14-15 0.21895243979469825 1105 16-17 0.34154488567038044 1105 18-19 0.12849674529685728 1105 20-21 0.13778834906266724 1105 22-23 0.20965261913147515 1105 24-25 0.10422146275221422 1105 26-27 0.2468838081456184 1105 28-29 0.39633120960518653 1105 30-31 0.4261812722491456 1105 32-33 0.33675470234979343 1105 34-35 0.2950474866336279 1105 36-37 0.2616571149828246 1105 38-39 0.35858204946231353 1105 40-41 0.2950195953618646 1105 42-43 0.06386918212718484 1105 44-45 0.11462135291156272 1105 46-47 0.06991827298558206 1105 48-49 0.07077448541510734 1105 50-51 0.09870611531196971 1105 52-53 0.00971002727446546 1105 54-55 0.10347043556193114 1105 56-57 0.18746034045094717 1105 58-59 0.18166967714524773 1105 60-61 0.1332871919653087 1105 62-63 0.03054206856158359 1105 64-65 0.03663958901637088 1105 66-67 0.9081109503585552 1105 68-69 1.8987525461156771 1105 70-71 2.1334317790503974 1105 72-73 1.2958906922416489 1105 74-75 0.9014309340120512 1105 76-77 0.4174676259532717 1105 78-79 0.4935662041188351 1105 80-81 0.7321888661229288 1105 82-83 0.7264726398544674 1105 84-85 0.5535746703597617 1105 86-87 0.3951781008234896 1105 88-89 0.5901775511879421 1105 90-91 0.8192989948737228 1105 92-93 0.7691501310868034 1105 94-95 0.8707480678390986 1105 96-97 0.5153267345274841 1105 98-99 0.7062219488046146 1105 100-101 0.8144605245974432 1106 1 0.4728577885357197 1106 2 0.4442796835244174 1106 3 0.47487122539464366 1106 4 0.29569730389399496 1106 5 0.5257325947025109 1106 6 0.47584673744779593 1106 7 0.47578530335380975 1106 8 0.5226380839708398 1106 9 0.7874295293351565 1106 10-11 0.5226637705511976 1106 12-13 0.6651984705920384 1106 14-15 0.8164063251950182 1106 16-17 0.7806638024678705 1106 18-19 0.8067738253596488 1106 20-21 0.7282269283405256 1106 22-23 0.7860851152068307 1106 24-25 0.6504832366925584 1106 26-27 0.8917278034360407 1106 28-29 0.9572122928077036 1106 30-31 0.9747733052161749 1106 32-33 1.033477622287002 1106 34-35 1.1092154615158947 1106 36-37 1.1734752076044828 1106 38-39 1.1262429599803667 1106 40-41 1.0894416911232483 1106 42-43 1.1426074081868336 1106 44-45 1.4019545554233375 1106 46-47 1.4236270641943705 1106 48-49 1.4458480725422689 1106 50-51 1.417804427713861 1106 52-53 1.2846315343388355 1106 54-55 1.2483144308523535 1106 56-57 1.308511164626772 1106 58-59 1.4078765060306502 1106 60-61 1.4396058732839911 1106 62-63 1.3981833558457275 1106 64-65 1.481203953223595 1106 66-67 1.896508713309892 1106 68-69 3.2712015257075144 1106 70-71 3.582877422692466 1106 72-73 2.691912081566606 1106 74-75 1.9008422369947873 1106 76-77 1.4170015741479354 1106 78-79 1.4179922245269978 1106 80-81 1.6240403182422405 1106 82-83 1.7758741312202453 1106 84-85 1.6671196075654144 1106 86-87 1.5874612640887982 1106 88-89 1.8845015307797794 1106 90-91 1.7087466008391878 1106 92-93 1.7906130431747158 1106 94-95 1.991725304887769 1106 96-97 1.7415090343704982 1106 98-99 1.8973031889929963 1106 100-101 1.9921734366853556 1107 1 0.2001326038843061 1107 2 0.18108341258224314 1107 3 0.15192220719972482 1107 4 0.10047617256630303 1107 5 0.1388464480622673 1107 6 0.2684120409967008 1107 7 0.06711028984273071 1107 8 0.22563123835743681 1107 9 0.257156605537638 1107 10-11 0.2269960980597574 1107 12-13 0.3192815186914757 1107 14-15 0.4679008757264498 1107 16-17 0.3091435808861789 1107 18-19 0.25010430635406067 1107 20-21 0.11213658425838702 1107 22-23 0.15275733642823042 1107 24-25 0.3005496661647342 1107 26-27 0.1489557803771362 1107 28-29 0.19736249097019964 1107 30-31 0.39063531206178936 1107 32-33 0.3208614270068679 1107 34-35 0.45619168201670135 1107 36-37 0.27447975633444344 1107 38-39 0.2711330698704728 1107 40-41 0.06658972751308312 1107 42-43 0.27740046349218517 1107 44-45 0.5871380809142437 1107 46-47 0.3955623010886242 1107 48-49 0.5140479464388577 1107 50-51 0.39324128593760577 1107 52-53 0.38703312864615924 1107 54-55 0.4671612602524604 1107 56-57 0.6116596964228407 1107 58-59 0.5068239929699381 1107 60-61 0.6439956228786592 1107 62-63 0.5864892917131392 1107 64-65 0.5369720580695656 1107 66-67 1.0942766830450736 1107 68-69 2.322182204864422 1107 70-71 2.4620289269259388 1107 72-73 1.5368661438977185 1107 74-75 1.035117367601881 1107 76-77 0.8054964399378797 1107 78-79 0.9338850816698532 1107 80-81 0.7917411829513021 1107 82-83 1.1219084043241914 1107 84-85 0.8501427115106495 1107 86-87 0.7244694608101092 1107 88-89 0.9434857228172469 1107 90-91 1.0439947543250447 1107 92-93 0.9336744285106917 1107 94-95 1.1960357894851334 1107 96-97 1.0711453611584645 1107 98-99 1.0499819856062196 1107 100-101 0.8306007470798775 1108 1 -0.07760424942631872 1108 2 -0.17109244434771043 1108 3 -0.0515523510289313 1108 4 -0.03450998881329781 1108 5 0.07141191538183733 1108 6 0.12399858559964372 1108 7 0.04514094770945576 1108 8 0.01207364840640679 1108 9 -0.1509570840514627 1108 10-11 -6.566590192278454E-4 1108 12-13 -0.08422710383354826 1108 14-15 0.06909738413607158 1108 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0.7913675334585761 2316 74-75 0.1840404860689766 2316 76-77 -0.12447779411592208 2316 78-79 -0.13960839808305892 2316 80-81 -0.4442134509809854 2316 82-83 -0.2832735887673863 2316 84-85 -0.27025806709349 2316 86-87 -0.09772233234222583 2316 88-89 -0.09706222547083243 2316 90-91 -0.009492649615228999 2316 92-93 -0.02893209511184125 2316 94-95 0.16090431565050878 2316 96-97 -0.006417562882628403 2316 98-99 -0.023918348563476854 2316 100-101 -0.1526088572046902 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 25.0 4 50.0 5 151.0 6 312.0 7 700.0 8 1395.0 9 2421.0 10 3802.0 11 5964.0 12 8070.0 13 10449.0 14 12613.0 15 16072.0 16 20026.0 17 23614.0 18 20665.0 19 15261.0 20 14348.0 21 14483.0 22 15318.0 23 17013.0 24 19790.0 25 23951.0 26 28295.0 27 34630.0 28 42213.0 29 50489.0 30 58710.0 31 69524.0 32 82647.0 33 99853.0 34 125920.0 35 159586.0 36 187432.0 37 191565.0 38 101205.0 39 7530.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.74940231964592 18.807210394253655 14.12948664103641 21.31390064506402 2 16.46580939143779 21.79711452061802 37.92113561483865 23.81594047310554 3 20.171240619717704 26.775390015180715 29.524741335687597 23.52862802941398 4 13.090464485158101 17.19591655188053 35.406100678488684 34.307518284472685 5 13.466079266696587 38.963001742147384 31.789176614985426 15.781742376170598 6 30.995574983042818 35.68167870716293 17.221498476545257 16.101247833248998 7 28.526738160429638 34.56867216608337 19.225730218148612 17.67885945533838 8 28.071586175061253 32.48684305264057 19.05333231949193 20.38823845280624 9 28.591135033581256 16.262262826719926 19.380901785011343 35.76570035468748 10-11 26.05580254855504 26.738395945890474 25.153842582280966 22.051958923273514 12-13 27.890945207479728 24.418897285977966 26.39494595574852 21.29521155079379 14-15 24.08005668539806 25.026932966017696 26.563609239504554 24.32940110907969 16-17 22.017344821182 28.86291696610977 26.626480729322278 22.493257483385953 18-19 21.722647217054885 26.34566463214199 30.70066445886926 21.231023691933867 20-21 23.117333525335155 25.076256798849606 30.34737974708246 21.459029928732782 22-23 22.42067644305856 25.37869331544307 30.215759805719276 21.98487043577909 24-25 22.256476787662706 25.727653438153876 30.59463540532893 21.421234368854492 26-27 22.07423552136153 25.75159251312753 30.828658608184067 21.345513357326872 28-29 21.350161311866355 26.203513701835647 31.08869444211732 21.35763054418068 30-31 21.412847055514415 26.213325357153312 30.808062626022693 21.565764961309583 32-33 21.193011332628565 26.348378231609292 30.458836536539835 21.99977389922231 34-35 22.12291656923213 26.069881675499424 30.200388212838075 21.606813542430373 36-37 22.182848828397344 25.75976503952496 30.37281787531871 21.684568256758986 38-39 21.930728865487396 25.787350327408003 30.593510575633893 21.688410231470705 40-41 21.60689264927786 25.94588677516706 30.85504688510455 21.59217369045053 42-43 21.541140058048065 26.314190451137264 29.989265453183467 22.155404037631204 44-45 21.464496010093235 26.401519374278614 29.729613214972666 22.40437140065548 46-47 21.608360733086386 26.69540286982594 29.067941396466317 22.628295000621357 48-49 22.024822877757785 26.599337145035644 28.8423747599163 22.533465217290267 50-51 21.765006604192333 26.975044168272532 28.804314514472306 22.45563471306283 52-53 21.998769679156972 27.103860426262067 28.382969854445705 22.51440004013526 54-55 21.06163466960686 26.71364244653363 28.879365052645756 23.345357831213757 56-57 21.68147832774355 27.27239376749086 28.175498410572175 22.870629494193416 58-59 21.85177757348735 26.475063631383495 28.358868889065363 23.31428990606379 60-61 21.647303306666092 26.531055850462458 28.96135717592842 22.86028366694303 62-63 21.773825072960847 26.489851427017957 28.580061114172878 23.15626238584832 64-65 21.24373373816943 26.671471403571996 28.61537105320977 23.46942380504881 66-67 21.078745721623203 26.971035595488384 28.436189044187792 23.51402963870062 68-69 20.920172000955485 26.832459892740978 28.798132005023398 23.449236101280142 70-71 20.93241025564802 27.14247686506711 28.243028779403843 23.682084099881024 72-73 21.23722223982515 26.83691994884514 28.01480308951629 23.91105472181342 74-75 21.257135587778585 27.37083921445096 27.386791430496466 23.985233767273996 76-77 21.29446084941316 27.10988576801841 27.648905100154074 23.946748282414358 78-79 21.22919839963231 27.225934004852515 27.648333634470557 23.896533961044618 80-81 21.079964227963963 27.84731208409793 27.36648235589407 23.706241332044033 82-83 21.229860395916685 27.59072347638493 26.893190915388683 24.286225212309706 84-85 20.981278743603607 27.216258218286022 26.832600818993985 24.969862219116386 86-87 21.28837026833701 27.096742501576294 27.033623380722023 24.58126384936467 88-89 21.209055416413765 27.093272700111836 26.869632741939608 24.828039141534788 90-91 20.937555514882025 27.325400649221308 27.080832359860253 24.656211476036415 92-93 21.03906346205997 27.13965502274793 27.04951788914243 24.771763626049665 94-95 20.735615232188344 26.951794592064704 27.217191180159567 25.095398995587388 96-97 20.92553165356482 26.73937206041352 27.016147892852587 25.318948393169073 98-99 20.79028370271635 26.89049614240088 26.658202281749606 25.66101787313316 100-101 20.642140094605953 26.882041356624338 26.990927707673123 25.48489084109659 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3567.0 1 2342.5 2 1499.5 3 1983.5 4 1795.0 5 1666.5 6 2819.5 7 5631.0 8 7908.0 9 8040.0 10 6372.0 11 4694.0 12 4307.0 13 4211.5 14 4090.5 15 4086.0 16 4065.5 17 4086.0 18 4237.5 19 4379.0 20 4650.0 21 5077.5 22 5552.0 23 6182.0 24 6866.0 25 7580.5 26 8788.5 27 10158.5 28 11410.0 29 12774.5 30 14317.0 31 16028.5 32 18184.5 33 19862.5 34 21430.0 35 22663.5 36 24005.5 37 26568.5 38 27932.5 39 27681.0 40 28358.5 41 32012.5 42 36069.0 43 39191.5 44 41621.0 45 43785.0 46 46883.5 47 52774.0 48 67609.0 49 70406.5 50 61577.5 51 58265.5 52 54811.0 53 54297.5 54 54407.5 55 51654.0 56 47314.0 57 43110.5 58 39216.5 59 35118.5 60 31044.5 61 26040.5 62 20904.0 63 16483.0 64 12853.0 65 10352.0 66 7949.0 67 6390.0 68 4625.5 69 2870.0 70 2277.0 71 1868.0 72 1552.0 73 1370.0 74 1100.0 75 885.0 76 599.5 77 330.5 78 173.0 79 91.0 80 56.5 81 37.5 82 27.0 83 14.0 84 4.0 85 3.0 86 1.0 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023753496575257202 2 0.025704916973791077 3 6.729035857013372E-5 4 0.0 5 0.0 6 6.729035857013372E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.3645179285066856E-4 18-19 3.364517928506686E-5 20-21 0.0 22-23 0.0 24-25 1.0093553785520056E-4 26-27 1.6822589642533428E-4 28-29 3.364517928506686E-5 30-31 3.7009697213573545E-4 32-33 0.002287872191384546 34-35 0.003801905259212555 36-37 0.10712625084365286 38-39 0.09969066622165311 40-41 0.10880850980790621 42-43 0.11018796215859394 44-45 0.10352621666015072 46-47 0.09706634223741788 48-49 0.09814298797454002 50-51 0.09649437418957174 52-53 0.07526426606069456 54-55 0.06288284008378996 56-57 0.03361153410578179 58-59 0.013121619921176074 60-61 0.00868045625554725 62-63 0.0017495493228234765 64-65 0.00427293776920349 66-67 0.15661830957198622 68-69 0.41864696584408684 70-71 0.40212718281511906 72-73 0.1855868089364288 74-75 0.027589047013754822 76-77 0.029338596336578298 78-79 0.00427293776920349 80-81 0.0013458071714026743 82-83 1.0093553785520056E-4 84-85 3.7009697213573545E-4 86-87 0.001211226454262407 88-89 2.0187107571040112E-4 90-91 6.056132271312035E-4 92-93 0.001884130039963744 94-95 0.0773502671763687 96-97 0.015981460160406757 98-99 0.017731009483230233 100-101 0.009689811634099255 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1486097.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.70146049938874 #Duplication Level Percentage of deduplicated Percentage of total 1 72.95660283310298 30.423968912142364 2 13.506973863445756 11.265210740655188 3 5.132934323109536 6.42152573763327 4 2.5794659506543773 4.302699898029269 5 1.4912980517338135 3.109465339859651 6 0.9084259135932098 2.2729612411397015 7 0.5926792082810507 1.7300912015058858 8 0.4220459514784666 1.4079946059604966 9 0.3173467947958902 1.1910442345009593 >10 1.7589207103426423 13.847456688228247 >50 0.18769685091556287 5.492019962050172 >100 0.13256269816474814 10.471933603127912 >500 0.0085368280277554 2.322653140902772 >1k 0.0038657334465307474 2.91703579059908 >5k 3.221444538775623E-4 1.0001534245652643 >10k+ 3.221444538775623E-4 1.8237854790998023 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15047 1.012518025404802 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12105 0.8145497904914687 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9388 0.6317218862564153 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5502 0.37023155285287573 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3799 0.255636072207938 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3437 0.23127696240554957 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3225 0.21701140638868124 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2973 0.20005423602900752 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2690 0.18101106455365967 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2178 0.14655840096575123 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1890 0.12717877769755273 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1856 0.12489090550616816 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1566 0.1053767015208294 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1551 0.10436734614227738 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1526 0.10268508717802403 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1512 0.10174302215804217 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1490 0.10026263426949923 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 6.729035857013371E-5 0.0 6 0.0 0.0 0.0 6.729035857013371E-5 0.0 7 0.0 0.0 0.0 6.729035857013371E-5 0.0 8 0.0 0.0 0.0 6.729035857013371E-5 0.0 9 0.0 0.0 0.0 6.729035857013371E-5 0.0 10-11 0.0 0.0 0.0 1.3458071714026742E-4 0.0 12-13 0.0 0.0 0.0 2.6916143428053484E-4 0.0 14-15 0.0 0.0 0.0 3.3645179285066856E-4 0.0 16-17 0.0 0.0 0.0 3.700969721357354E-4 0.0 18-19 0.0 0.0 0.0 6.392584064162703E-4 0.0 20-21 0.0 0.0 0.0 0.0010430005578370726 0.0 22-23 0.0 0.0 0.0 0.0022542270120994795 0.0 24-25 0.0 0.0 0.0 0.0058879063748867 0.0 26-27 0.0 0.0 0.0 0.01123748988121233 0.0 28-29 0.0 0.0 0.0 0.026108659125211882 0.0 30-31 0.0 0.0 0.0 0.06641558390872197 0.0 32-33 0.0 0.0 0.0 0.136363911642376 0.0 34-35 0.0 0.0 0.0 0.24187519388034562 0.0 36-37 0.0 0.0 0.0 0.4085870572378519 0.0 38-39 0.0 0.0 0.0 0.6571239966166408 0.0 40-41 0.0 0.0 0.0 0.9381285340055191 0.0 42-43 0.0 0.0 0.0 1.2008300938633212 0.0 44-45 3.3645179285066855E-5 0.0 0.0 1.5045787724489048 0.0 46-47 6.729035857013371E-5 0.0 0.0 1.8564400574121338 0.0 48-49 6.729035857013371E-5 0.0 0.0 2.23949042357262 0.0 50-51 6.729035857013371E-5 0.0 0.0 2.6527541607310963 0.0 52-53 6.729035857013371E-5 0.0 0.0 3.0388662382065235 0.0 54-55 6.729035857013371E-5 0.0 0.0 3.424742799426955 0.0 56-57 6.729035857013371E-5 0.0 0.0 3.859169354355738 0.0 58-59 6.729035857013371E-5 0.0 0.0 4.320512052712575 0.0 60-61 6.729035857013371E-5 0.0 0.0 4.821926159597927 0.0 62-63 6.729035857013371E-5 0.0 0.0 5.281048276121949 0.0 64-65 6.729035857013371E-5 0.0 0.0 5.707164471767321 0.0 66-67 6.729035857013371E-5 0.0 0.0 6.156462195940104 0.0 68-69 6.729035857013371E-5 0.0 0.0 6.656395914936912 0.0 70-71 6.729035857013371E-5 0.0 0.0 7.242831389875628 0.0 72-73 6.729035857013371E-5 0.0 0.0 7.8425567106319445 0.0 74-75 6.729035857013371E-5 0.0 0.0 8.425055699594306 0.0 76-77 6.729035857013371E-5 0.0 0.0 9.019532372382153 0.0 78-79 6.729035857013371E-5 0.0 0.0 9.677261982225925 0.0 80-81 6.729035857013371E-5 0.0 0.0 10.396562270161368 0.0 82-83 6.729035857013371E-5 0.0 0.0 11.110277458335492 0.0 84-85 6.729035857013371E-5 0.0 0.0 11.799969988500077 3.3645179285066855E-5 86-87 6.729035857013371E-5 0.0 0.0 12.481991417787668 6.729035857013371E-5 88-89 6.729035857013371E-5 0.0 0.0 13.181508340303493 6.729035857013371E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTATC 60 1.50081E-6 47.462112 1 CGTATCA 95 3.5146273E-5 29.971025 2 GTACATG 13845 0.0 25.985035 1 TACATGG 13945 0.0 25.556149 2 ATGGGCG 205 9.713403E-10 25.455479 5 TAAGGTG 530 0.0 25.062515 5 ACATGGG 13990 0.0 24.14375 3 GTAAGGT 395 0.0 24.02013 4 ATGGGAG 2490 0.0 22.862534 5 CATGGGG 4560 0.0 22.471466 4 GGTATCA 15220 0.0 22.203041 1 GTATCAA 19725 0.0 22.161018 1 GAGTACT 10355 0.0 21.875889 12-13 CATGGGA 8795 0.0 21.683662 4 AGTACTT 10195 0.0 21.591022 12-13 AGGTAAG 375 0.0 21.512539 2 ATGGGAT 3065 0.0 21.359499 5 ACCGTGC 135 3.8293414E-4 21.084337 8 GTACTTT 10975 0.0 20.748138 14-15 GTATAGC 300 1.6916601E-10 20.566916 1 >>END_MODULE