FastQCFastQC Report
Fri 27 May 2016
SRR939743_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939743_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3139671
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50060.15944345761068596No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38200.12166879905569723No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47100.042.7021561
GTATCAA78700.036.465781
ATCAACG94400.031.1572553
TCAACGC95900.030.7198724
TATCAAC95750.030.6164282
CAACGCA97700.030.0572625
AACGCAG100900.029.2445966
GTACATG88350.026.737921
TACATGG90100.026.3238892
ACGCAGA112150.026.0149067
CGCAGAG115050.025.3595688
ACATGGG95400.024.6148013
GCAGAGT121050.023.0447929
CATGGGG60200.020.5679234
GAGTACT67500.020.06416112-13
CAGAGTA117850.019.66270610-11
AGAGTAC105850.019.5614810-11
GGACCGA6450.019.1231776
GTACTTT71800.018.6661314-15
AGTACTT73600.017.30554212-13
Produced by FastQC (version 0.11.5)