FastQCFastQC Report
Fri 27 May 2016
SRR939743_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939743_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3139671
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58030.18482828296340603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47640.15173564363909467No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32150.102399264126719No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49000.040.7183721
GTATCAA79150.036.053761
ATCAACG95050.029.2207683
TCAACGC96200.028.9186274
CAACGCA98050.028.3249055
TATCAAC100100.027.9839172
AACGCAG102700.027.2537446
ACGCAGA117050.023.7917427
CGCAGAG120250.023.0410588
GTACATG90700.022.4658371
TACATGG91350.021.4237582
GCAGAGT132400.020.4639769
ACATGGG92300.020.3844813
GAGTACT71350.020.010712-13
AGAGTAC116600.018.7714310-11
GTACTTT76000.018.47650314-15
CAGAGTA126850.017.88611410-11
GGACCGA7200.017.6701986
GTATAGG8850.017.5880131
AGTACTT77700.017.4353412-13
Produced by FastQC (version 0.11.5)