##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939743_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3139671 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.437426405505544 31.0 31.0 34.0 30.0 34.0 2 31.65823680251848 31.0 31.0 34.0 30.0 34.0 3 31.72296683314908 31.0 31.0 34.0 30.0 34.0 4 35.52224803172052 37.0 35.0 37.0 33.0 37.0 5 35.246477099033626 37.0 35.0 37.0 33.0 37.0 6 35.258246485061655 37.0 35.0 37.0 32.0 37.0 7 35.18233120604038 37.0 35.0 37.0 32.0 37.0 8 35.12024062393799 37.0 35.0 37.0 32.0 37.0 9 36.745689596139215 39.0 35.0 39.0 32.0 39.0 10-11 36.59879968315151 39.0 35.0 39.0 32.0 39.0 12-13 36.536692379551866 38.0 35.0 39.0 32.0 39.0 14-15 37.61527067644985 39.0 36.0 41.0 32.0 41.0 16-17 37.52895908520351 39.0 36.0 41.0 32.0 41.0 18-19 37.479269802472935 39.0 36.0 41.0 32.0 41.0 20-21 37.388003711216875 39.0 36.0 41.0 32.0 41.0 22-23 37.24414516680251 39.0 36.0 40.0 31.0 41.0 24-25 37.087360586507316 39.0 36.0 40.0 31.0 41.0 26-27 36.79326321133647 39.0 36.0 40.0 30.0 41.0 28-29 36.57036485669995 38.0 35.0 40.0 30.0 41.0 30-31 36.33778666618254 38.0 35.0 40.0 30.0 41.0 32-33 36.32915773659087 38.0 35.0 40.0 30.0 41.0 34-35 36.442054756692656 38.0 35.0 40.0 30.0 41.0 36-37 36.430622667152065 38.0 35.0 40.0 30.0 41.0 38-39 36.30778304478399 38.0 35.0 40.0 30.0 41.0 40-41 36.15086246297781 38.0 35.0 40.0 30.0 41.0 42-43 35.971590972429915 38.0 35.0 40.0 29.0 41.0 44-45 35.75572631654718 38.0 34.5 40.0 28.5 41.0 46-47 35.498690627138956 38.0 34.0 40.0 27.5 41.0 48-49 35.221944273779 38.0 34.0 40.0 27.0 41.0 50-51 34.96313929070912 38.0 33.5 40.0 26.0 41.0 52-53 34.68173655774761 38.0 33.0 40.0 26.0 41.0 54-55 34.37418841018693 38.0 33.0 40.0 25.0 41.0 56-57 32.945002358527375 36.5 31.5 39.5 16.5 41.0 58-59 29.26297054691399 34.5 23.5 38.5 8.0 40.0 60-61 29.293084848699117 34.0 24.0 38.0 9.5 40.0 62-63 31.34011270607653 35.0 29.5 38.0 13.0 40.0 64-65 30.866090427946112 35.0 29.0 38.0 6.5 40.0 66-67 25.180440721336723 28.0 18.0 36.0 2.0 39.0 68-69 17.330809820519413 13.0 7.0 29.0 2.0 35.5 70-71 16.20642783909524 13.0 7.5 25.5 2.0 33.0 72-73 18.238832348994528 17.0 7.0 30.0 2.0 34.0 74-75 18.977811369407814 17.5 7.5 30.5 2.0 35.0 76-77 20.01597476296083 22.5 8.5 30.5 2.0 34.5 78-79 23.140229661005883 27.5 12.0 33.5 2.0 36.0 80-81 25.910539511942495 31.5 22.0 34.5 2.0 36.0 82-83 27.036903229669605 33.0 26.0 35.0 2.0 36.0 84-85 27.06756265226516 33.0 26.0 35.0 2.0 36.0 86-87 26.789372517056727 33.0 25.5 35.0 2.0 35.5 88-89 26.353059604015833 33.0 25.0 35.0 2.0 35.0 90-91 24.592227656974252 31.5 12.0 34.5 2.0 35.0 92-93 22.29581411555542 30.5 2.0 34.0 2.0 35.0 94-95 17.720773131961916 20.0 2.0 33.0 2.0 35.0 96-97 16.34407203812119 13.5 2.0 32.5 2.0 34.5 98-99 15.208078808257298 2.0 2.0 31.5 2.0 34.0 100-101 14.581319666933254 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4361440460431645 1101 2 0.42326376152933065 1101 3 0.44057762664115785 1101 4 0.372013349530377 1101 5 0.4678382375133623 1101 6 0.45464705132287264 1101 7 0.46143758246657995 1101 8 0.46307696154571687 1101 9 0.5755779984421352 1101 10-11 0.5101547777230522 1101 12-13 0.5153668633268822 1101 14-15 0.5749383765748277 1101 16-17 0.5398493754389833 1101 18-19 0.6455837518626382 1101 20-21 0.6480099770391377 1101 22-23 0.6245689423494767 1101 24-25 0.5650649384418074 1101 26-27 0.6662447429070539 1101 28-29 0.599220224274795 1101 30-31 0.7495900583783808 1101 32-33 0.5877215020649587 1101 34-35 0.8162663276254776 1101 36-37 0.7985785438252222 1101 38-39 0.6888901529040581 1101 40-41 0.6266515108872923 1101 42-43 0.8361904712796147 1101 44-45 0.6039278634800098 1101 46-47 0.5927390925424874 1101 48-49 0.6371119878577858 1101 50-51 0.6694123883631065 1101 52-53 0.756024624051868 1101 54-55 0.6324768878563631 1101 56-57 1.2309065944627733 1101 58-59 -8.31280066266375 1101 60-61 -9.471628257264655 1101 62-63 -2.467981142417983 1101 64-65 0.03485610008125306 1101 66-67 -1.0277521059201007 1101 68-69 -3.654301408763301 1101 70-71 -4.108932446146852 1101 72-73 -6.629318887916227 1101 74-75 -7.34446054599459 1101 76-77 -8.36504896170327 1101 78-79 -11.82314789964145 1101 80-81 -7.803029616895877 1101 82-83 -3.629624045565585 1101 84-85 -2.701170839991075 1101 86-87 -2.451873183967333 1101 88-89 -2.657235040448178 1101 90-91 -8.594126488742795 1101 92-93 -14.319656028229982 1101 94-95 -12.344773188638342 1101 96-97 -12.3350417919119 1101 98-99 -11.989683711834433 1101 100-101 -11.835200256245855 1102 1 0.46676715481780207 1102 2 0.5339964704991296 1102 3 0.4744138327252827 1102 4 0.3982491116561633 1102 5 0.5126945084248788 1102 6 0.4706694560325815 1102 7 0.541976116208545 1102 8 0.5925971214224148 1102 9 0.6522402438352088 1102 10-11 0.6282735985085353 1102 12-13 0.7244497533211884 1102 14-15 0.7908566040157012 1102 16-17 0.8457986810405416 1102 18-19 0.8172687794700195 1102 20-21 0.9071460192507246 1102 22-23 0.8622914263836279 1102 24-25 0.8361319570870904 1102 26-27 0.8409840035473195 1102 28-29 0.8018788044805163 1102 30-31 0.9154846177346485 1102 32-33 0.7354263710127 1102 34-35 0.6865306239294284 1102 36-37 0.8508112331389341 1102 38-39 0.765798772556586 1102 40-41 0.8340171857562027 1102 42-43 0.9329174094372519 1102 44-45 0.914204335788348 1102 46-47 0.7715564530152221 1102 48-49 0.8738554371297269 1102 50-51 0.8255065027916899 1102 52-53 0.7365574225241787 1102 54-55 0.6939868470309349 1102 56-57 1.2839498954793456 1102 58-59 -8.784834760861258 1102 60-61 -9.978744165179382 1102 62-63 -2.6675775463769895 1102 64-65 0.23902792983690802 1102 66-67 -1.0682367819796852 1102 68-69 -3.799200707660244 1102 70-71 -4.380584031477703 1102 72-73 -6.88804053670124 1102 74-75 -7.551490047898538 1102 76-77 -8.462725719751898 1102 78-79 -11.619949584787532 1102 80-81 -7.601832049511845 1102 82-83 -3.3457298136312836 1102 84-85 -2.503137902849094 1102 86-87 -2.195805511016644 1102 88-89 -2.359008019627261 1102 90-91 -8.376964811760718 1102 92-93 -14.703204070672054 1102 94-95 -12.758271724663643 1102 96-97 -12.394866712708634 1102 98-99 -11.81875921329356 1102 100-101 -11.629796151300653 1103 1 0.3456304818835747 1103 2 0.3458018891354335 1103 3 0.3063562000044371 1103 4 0.1927159440686026 1103 5 0.25924955317142917 1103 6 0.31886841346926076 1103 7 0.3541680478841016 1103 8 0.44301056414154516 1103 9 0.4322279651835501 1103 10-11 0.36326641325317865 1103 12-13 0.47399170831976534 1103 14-15 0.573752790901267 1103 16-17 0.4705030356338895 1103 18-19 0.5343356243855197 1103 20-21 0.494877566408924 1103 22-23 0.42070118069838003 1103 24-25 0.4273550454404784 1103 26-27 0.32362257518428095 1103 28-29 0.46929846026150557 1103 30-31 0.5321020535802958 1103 32-33 0.47648477555740243 1103 34-35 0.5449689709920946 1103 36-37 0.2548241561793603 1103 38-39 0.40126488043240016 1103 40-41 0.3378259120945515 1103 42-43 0.5003921869067298 1103 44-45 0.534415000422463 1103 46-47 0.33977069464819465 1103 48-49 0.4656564917152295 1103 50-51 0.5700113653621699 1103 52-53 0.4866214406247735 1103 54-55 0.4146881968811371 1103 56-57 0.9149911403679241 1103 58-59 -6.346466459018018 1103 60-61 -8.51965904494958 1103 62-63 -2.477160765499626 1103 64-65 -0.143464391998414 1103 66-67 -1.6807764952427107 1103 68-69 -4.189433208577938 1103 70-71 -4.872167705038688 1103 72-73 -7.494750595417559 1103 74-75 -7.999221222369201 1103 76-77 -9.000747562447303 1103 78-79 -12.156735547531603 1103 80-81 -8.401340983593439 1103 82-83 -4.306515270642375 1103 84-85 -3.3619436276502057 1103 86-87 -3.17218102857143 1103 88-89 -3.4013613530567746 1103 90-91 -8.719446522019805 1103 92-93 -14.596682659973597 1103 94-95 -12.584968815456739 1103 96-97 -12.397427436732311 1103 98-99 -12.123695660050537 1103 100-101 -11.853587601768474 1104 1 0.16919130508518165 1104 2 0.18603212829312454 1104 3 0.1474275692996052 1104 4 0.21664949639666986 1104 5 0.0036915569393656256 1104 6 0.13044823420430163 1104 7 0.13059492909243886 1104 8 0.19797715265386984 1104 9 0.15049196335716175 1104 10-11 0.20923294316668972 1104 12-13 0.20588060902626637 1104 14-15 0.1806491011403466 1104 16-17 0.2805111463199452 1104 18-19 0.3179340863189779 1104 20-21 0.1993447185538102 1104 22-23 0.10261460484068863 1104 24-25 0.09061219805280984 1104 26-27 0.17768653867281614 1104 28-29 0.2066255344733463 1104 30-31 0.2940826749851908 1104 32-33 0.23000388012682293 1104 34-35 0.2704494728283038 1104 36-37 0.17812948176183596 1104 38-39 0.15148716640953097 1104 40-41 0.18896336425861193 1104 42-43 0.17383456767168326 1104 44-45 0.06278288278313937 1104 46-47 -0.013112909131670847 1104 48-49 -0.0014003454119020375 1104 50-51 0.006552825888583413 1104 52-53 0.036363072341025315 1104 54-55 -0.04611774686805603 1104 56-57 0.5580275092329074 1104 58-59 -6.699991431937235 1104 60-61 -8.82875095634817 1104 62-63 -2.915421449283471 1104 64-65 -0.5881894907187899 1104 66-67 -2.1690775207558914 1104 68-69 -4.643211866956316 1104 70-71 -5.152344233129877 1104 72-73 -8.015433606976767 1104 74-75 -8.962801887875633 1104 76-77 -9.46239645696198 1104 78-79 -12.635854680282652 1104 80-81 -8.86850481437089 1104 82-83 -5.126550087824224 1104 84-85 -4.159872633658448 1104 86-87 -3.945334039568362 1104 88-89 -4.085470877972789 1104 90-91 -9.818086665326092 1104 92-93 -15.396344861500689 1104 94-95 -12.83546301010914 1104 96-97 -12.636297217965652 1104 98-99 -11.979168154510749 1104 100-101 -11.504283522003629 1105 1 0.1659403059749991 1105 2 0.14318922772810794 1105 3 0.14517791609462805 1105 4 0.11991939596455126 1105 5 0.050555835143093475 1105 6 0.08392137818451317 1105 7 0.12431246166606513 1105 8 0.15563625787628155 1105 9 0.16909747761299343 1105 10-11 0.2925439336022606 1105 12-13 0.25742115970890467 1105 14-15 0.25447834762948673 1105 16-17 0.29608235461124366 1105 18-19 0.25007912144906896 1105 20-21 0.21540602310845713 1105 22-23 0.31231913857723015 1105 24-25 0.25275701476995494 1105 26-27 0.3587618233749339 1105 28-29 0.30118874644362137 1105 30-31 0.33262129297134635 1105 32-33 0.33021865571011233 1105 34-35 0.2545062746369666 1105 36-37 0.17075206902983808 1105 38-39 0.3134595744929456 1105 40-41 0.40794515928271835 1105 42-43 0.285167163445756 1105 44-45 0.4376550166293285 1105 46-47 0.3645979049225616 1105 48-49 0.41160139810802576 1105 50-51 0.3852941362354656 1105 52-53 0.4834004298953758 1105 54-55 0.3776665013976199 1105 56-57 1.0014279475033945 1105 58-59 -6.571376095978589 1105 60-61 -8.678310923080613 1105 62-63 -2.4583751041229327 1105 64-65 0.08508221806488336 1105 66-67 -1.2865452842109022 1105 68-69 -3.918076760499787 1105 70-71 -4.424434508526229 1105 72-73 -5.764485660453886 1105 74-75 -6.513387612887904 1105 76-77 -8.395520394778615 1105 78-79 -11.745031480220188 1105 80-81 -7.6161568323516065 1105 82-83 -3.4324488364041095 1105 84-85 -2.49386113413156 1105 86-87 -2.2575753191310675 1105 88-89 -2.299672998332415 1105 90-91 -7.798863833573186 1105 92-93 -11.116599599771485 1105 94-95 -11.596026800959667 1105 96-97 -12.094984153811065 1105 98-99 -11.52732622837697 1105 100-101 -11.110223855030037 1106 1 0.08230112220574881 1106 2 0.1417486900609255 1106 3 0.0476853173174554 1106 4 0.03376901914251107 1106 5 0.06758866380532425 1106 6 -0.04712533859154178 1106 7 0.04915123734856053 1106 8 0.14511357200149178 1106 9 0.10088639144687761 1106 10-11 0.056593736784826376 1106 12-13 0.07065475360534634 1106 14-15 0.14843338368048364 1106 16-17 0.059569106815871464 1106 18-19 0.17718959677284118 1106 20-21 0.11570575653566095 1106 22-23 0.14162973380868493 1106 24-25 0.22872969070013482 1106 26-27 0.2010592573792067 1106 28-29 0.2806706536911605 1106 30-31 0.19874036215578172 1106 32-33 0.27454081960016197 1106 34-35 0.24785984060264354 1106 36-37 0.19664990997616627 1106 38-39 0.23772993340038084 1106 40-41 0.2420137856815856 1106 42-43 0.261653167300544 1106 44-45 0.28792065935882505 1106 46-47 0.18110946428044628 1106 48-49 0.3363208742345236 1106 50-51 0.19695662473478137 1106 52-53 0.19783091849770784 1106 54-55 0.07389298668604027 1106 56-57 1.08997236414109 1106 58-59 -6.23558773160233 1106 60-61 -9.004471437682021 1106 62-63 -2.4921685366734323 1106 64-65 -0.23757926023368725 1106 66-67 -1.7002049581707617 1106 68-69 -4.306926240589819 1106 70-71 -4.861799916448433 1106 72-73 -6.360885961797052 1106 74-75 -6.905765283445131 1106 76-77 -8.634734963704783 1106 78-79 -12.071091117835714 1106 80-81 -8.040277664379264 1106 82-83 -4.143556644568992 1106 84-85 -3.2680512207140637 1106 86-87 -3.1061375001344267 1106 88-89 -3.163028681999357 1106 90-91 -8.657558070420901 1106 92-93 -12.069410454707517 1106 94-95 -12.104988218732045 1106 96-97 -12.377846262841203 1106 98-99 -11.838571257860236 1106 100-101 -11.488016931291607 1107 1 0.037697940614368264 1107 2 0.09643449323664655 1107 3 0.0330933622823828 1107 4 0.03846692938876117 1107 5 0.047627773311944566 1107 6 0.10835476178707637 1107 7 0.12935654391426255 1107 8 0.02488852724201962 1107 9 0.08042654289532436 1107 10-11 0.2045784598631002 1107 12-13 0.10530097032924601 1107 14-15 0.09958924203509412 1107 16-17 0.13859544152867898 1107 18-19 0.13402789033627727 1107 20-21 0.05994939482041417 1107 22-23 0.10926111386796578 1107 24-25 0.20339105960624693 1107 26-27 0.29753408201012377 1107 28-29 0.28459591270909357 1107 30-31 0.21452908276862104 1107 32-33 0.2270163486424579 1107 34-35 0.11398957703539736 1107 36-37 0.07458660834134179 1107 38-39 0.10857445660193576 1107 40-41 0.006750842122535516 1107 42-43 0.12332658457622614 1107 44-45 0.16853742288205353 1107 46-47 0.24861537106333742 1107 48-49 0.26329707833772176 1107 50-51 0.28888524438198715 1107 52-53 0.16239755874895678 1107 54-55 0.06438581783276476 1107 56-57 0.6944539980819755 1107 58-59 -6.764718443938335 1107 60-61 -9.291140452386607 1107 62-63 -2.9608115908837576 1107 64-65 -0.4907733255678437 1107 66-67 -1.8265178415613263 1107 68-69 -4.250264233310903 1107 70-71 -4.845527646776279 1107 72-73 -6.397065740787982 1107 74-75 -7.13485197007012 1107 76-77 -9.39239947311161 1107 78-79 -12.591396326077328 1107 80-81 -8.652051056707052 1107 82-83 -4.678109490097452 1107 84-85 -3.748299085467078 1107 86-87 -3.508216614337126 1107 88-89 -3.593535197804588 1107 90-91 -8.60875080030849 1107 92-93 -11.923383408245716 1107 94-95 -12.236393959684762 1107 96-97 -12.720545332431175 1107 98-99 -11.978108180756541 1107 100-101 -11.442600925010606 1108 1 -0.023326297778030636 1108 2 -0.06530398954989991 1108 3 -0.01724730933512575 1108 4 0.01414763990917578 1108 5 -0.16041773513750712 1108 6 -0.08003212330471854 1108 7 -0.06060806022333054 1108 8 -0.08227660403722581 1108 9 -0.1345372477520712 1108 10-11 -0.005123676181383985 1108 12-13 -0.10505435315061362 1108 14-15 0.005660093415862377 1108 16-17 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5.6668722770202855 2312 80-81 3.0897907098611057 2312 82-83 1.6375414004840962 2312 84-85 1.3299551859115972 2312 86-87 1.1255885432663995 2312 88-89 1.306653501648647 2312 90-91 2.8662783351049264 2312 92-93 4.792687268276769 2312 94-95 8.962462044207221 2312 96-97 9.560280298019233 2312 98-99 10.26695486428445 2312 100-101 9.174113589689615 2313 1 -0.23885406506018825 2313 2 -0.28403483978143385 2313 3 -0.25742069110868115 2313 4 -0.3052920756429742 2313 5 -0.39376498645782476 2313 6 -0.23594953255092577 2313 7 -0.40457016713413196 2313 8 -0.4107495092731597 2313 9 -0.351920547047861 2313 10-11 -0.35385717678802564 2313 12-13 -0.26608404049526513 2313 14-15 -0.49367439389193635 2313 16-17 -0.4407527881282576 2313 18-19 -0.46697208380311395 2313 20-21 -0.45061891042337265 2313 22-23 -0.48142449692254985 2313 24-25 -0.36836241864498476 2313 26-27 -0.5257792986358041 2313 28-29 -0.6317689545521219 2313 30-31 -0.52363645834604 2313 32-33 -0.40785395119277723 2313 34-35 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8.828348519355846 2314 1 -0.43179167094841375 2314 2 -0.4232132032403193 2314 3 -0.45874268107804994 2314 4 -0.2512312037036821 2314 5 -0.40900802915719225 2314 6 -0.33813349476215393 2314 7 -0.3909009086602637 2314 8 -0.5004841014842683 2314 9 -0.422437805192601 2314 10-11 -0.5293156985229288 2314 12-13 -0.4966732091347694 2314 14-15 -0.628401197591522 2314 16-17 -0.6559939140870981 2314 18-19 -0.6900236001786269 2314 20-21 -0.5734215777781984 2314 22-23 -0.49366910293419863 2314 24-25 -0.4886896389785207 2314 26-27 -0.5434080287998739 2314 28-29 -0.7516281161978569 2314 30-31 -0.8421819982310765 2314 32-33 -0.9054531635526715 2314 34-35 -0.897804686740578 2314 36-37 -0.8079017234864168 2314 38-39 -0.7284381149126702 2314 40-41 -0.7869539907591232 2314 42-43 -0.8453876581217514 2314 44-45 -0.7905618694997614 2314 46-47 -0.6206916900628272 2314 48-49 -0.8014142284975705 2314 50-51 -0.6618524852175298 2314 52-53 -0.8502793457978655 2314 54-55 -0.5347729445881981 2314 56-57 0.7214733446643251 2314 58-59 4.01751205892225 2314 60-61 3.751862408197553 2314 62-63 1.2142109926754117 2314 64-65 1.0342078547995186 2314 66-67 1.5749933749397265 2314 68-69 -0.21317261933920761 2314 70-71 2.499334593512792 2314 72-73 8.303786788570644 2314 74-75 9.81233261288682 2314 76-77 8.769012752121757 2314 78-79 6.438024405629989 2314 80-81 3.346031222726854 2314 82-83 1.7385421208631051 2314 84-85 1.3066128386521605 2314 86-87 1.3460592061988592 2314 88-89 1.4377769069831245 2314 90-91 2.6811581126695287 2314 92-93 4.946087383684425 2314 94-95 8.732463312221292 2314 96-97 9.388789205358645 2314 98-99 9.58343201797878 2314 100-101 8.856140041565727 2315 1 -0.7178266991562268 2315 2 -0.4623619167475894 2315 3 -0.4862254496537055 2315 4 -0.3644399575479227 2315 5 -0.6903431588088722 2315 6 -0.685003570424044 2315 7 -0.7757625159642245 2315 8 -0.800019484545146 2315 9 -1.0409065039444059 2315 10-11 -0.8010340093421391 2315 12-13 -0.7114712335855486 2315 14-15 -0.7190977821808744 2315 16-17 -0.847986013093113 2315 18-19 -0.8997557462577959 2315 20-21 -1.0057091972153245 2315 22-23 -0.8667438746854899 2315 24-25 -0.8298757002698665 2315 26-27 -0.7502666669660272 2315 28-29 -0.8274456315046592 2315 30-31 -0.915760982108317 2315 32-33 -0.8604953850531558 2315 34-35 -0.8293393961820783 2315 36-37 -0.8764705629765501 2315 38-39 -0.8682692188866312 2315 40-41 -0.560112062764226 2315 42-43 -0.7073283635776662 2315 44-45 -0.6531564969842307 2315 46-47 -0.72931196319945 2315 48-49 -0.6297980131157956 2315 50-51 -0.4537945138543762 2315 52-53 -0.5027673269044541 2315 54-55 -0.515982422172307 2315 56-57 0.9033373958433657 2315 58-59 4.327582568427097 2315 60-61 3.998309717529189 2315 62-63 1.4771650843117072 2315 64-65 1.4111205028053249 2315 66-67 2.1506392275694495 2315 68-69 6.34503491156509 2315 70-71 5.273691948594404 2315 72-73 9.385511404547454 2315 74-75 10.526580214120187 2315 76-77 9.273974329754385 2315 78-79 6.715289751521514 2315 80-81 3.69363020066983 2315 82-83 1.9758138220327162 2315 84-85 1.3724409394063741 2315 86-87 1.4714609219234625 2315 88-89 1.4900608976968002 2315 90-91 2.791624975006588 2315 92-93 4.788009564607364 2315 94-95 8.463229373833407 2315 96-97 9.456135606106042 2315 98-99 9.506594149743542 2315 100-101 8.81900647246561 2316 1 -0.4790077612108625 2316 2 -0.4144199729239091 2316 3 -0.5662665957211708 2316 4 -0.34946111131549173 2316 5 -0.5186801737079278 2316 6 -0.5497719544138633 2316 7 -0.5999884730458405 2316 8 -0.5967497379816962 2316 9 -0.6577599374794119 2316 10-11 -0.52830596914675 2316 12-13 -0.6462143341449931 2316 14-15 -0.7005692541690678 2316 16-17 -0.7726799986263799 2316 18-19 -0.7306325037842143 2316 20-21 -0.6466044736984031 2316 22-23 -0.7749972854213141 2316 24-25 -2.0459365934384977 2316 26-27 -1.3230754721814435 2316 28-29 -1.0429299312579516 2316 30-31 -0.6427011708253048 2316 32-33 -0.5390624350758486 2316 34-35 -0.6468174412169105 2316 36-37 -0.4982459308284106 2316 38-39 -0.9755264560131494 2316 40-41 -0.7219490851913051 2316 42-43 -0.7639654522514192 2316 44-45 -0.7541881061116484 2316 46-47 -0.7845325452668206 2316 48-49 -0.9012128958183112 2316 50-51 -0.7843056779245856 2316 52-53 -0.7611042987861794 2316 54-55 -0.8610391942815951 2316 56-57 0.40472798451389025 2316 58-59 3.7332200958775275 2316 60-61 3.791770224402285 2316 62-63 1.2155876176447578 2316 64-65 1.0568603361992395 2316 66-67 6.145196138409595 2316 68-69 8.978675962134101 2316 70-71 5.654571287160081 2316 72-73 9.473448917587078 2316 74-75 10.484504757437552 2316 76-77 8.864750018085289 2316 78-79 6.332064140729326 2316 80-81 3.361562454841973 2316 82-83 1.7770835320314475 2316 84-85 1.4072291006094808 2316 86-87 1.2352598030429434 2316 88-89 1.373164446840864 2316 90-91 2.82849479814087 2316 92-93 4.7827605911081825 2316 94-95 8.8158027775144 2316 96-97 9.476833927785268 2316 98-99 9.736887377311625 2316 100-101 8.90880131210779 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 26.0 4 55.0 5 104.0 6 299.0 7 683.0 8 1662.0 9 2859.0 10 4570.0 11 7091.0 12 9889.0 13 13588.0 14 18407.0 15 25495.0 16 34087.0 17 44590.0 18 50796.0 19 54638.0 20 58408.0 21 58306.0 22 56343.0 23 53816.0 24 55029.0 25 58375.0 26 77328.0 27 112446.0 28 158862.0 29 209636.0 30 248158.0 31 260989.0 32 266023.0 33 272291.0 34 274066.0 35 262682.0 36 216608.0 37 130048.0 38 39683.0 39 1728.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.65736971579046 19.517798855821972 14.682980877483494 24.14185055090407 2 15.942780515306627 20.589140274375776 38.890679696383806 24.57739951393379 3 19.348488945754553 23.921322808301664 29.668237267415883 27.061950978527904 4 13.634454055854897 16.922346322273896 36.401075144497625 33.042124477373584 5 14.07132086933325 37.053368107003294 34.43552651162998 14.439784512033471 6 32.97536554820446 36.14168759244608 16.94468969330515 13.938257166044307 7 29.005395788284822 31.74864500133931 20.69172215814969 18.55423705222617 8 26.210918949529898 35.594193131041855 19.423657522261163 18.77123039716708 9 25.829298674924857 16.561066430208772 19.80239330808865 37.80724158677772 10-11 25.075445803079365 26.339113238297895 27.521800851108285 21.063640107514452 12-13 25.81918615039601 23.266546080783623 27.80794548218587 23.10632228663449 14-15 22.54209726615607 25.11980981401351 25.59196232335089 26.746130596479528 16-17 22.75155581588007 28.08942401926826 26.26727450105441 22.89174566379726 18-19 22.63368973950589 27.07292373192442 27.912959156486455 22.380427372083233 20-21 23.22789553427732 26.28812063429576 28.042619752196966 22.44136407922996 22-23 22.94670365143354 26.15579785270495 27.875388854437293 23.022109641424212 24-25 22.805144233265203 26.363781428054082 27.977262585793223 22.853811752887484 26-27 22.700728229863795 26.64146336491629 27.851893894513687 22.805914510706227 28-29 22.683284882470957 26.80174620581493 27.815057744488403 22.699911167225707 30-31 22.901777310173546 26.486960754053236 27.775213599256166 22.836048336517056 32-33 22.711503092129746 26.470519381927595 27.791154118537186 23.026823407405473 34-35 23.00268995679921 26.620411916456067 27.673828365435988 22.703069761308736 36-37 23.087918200690456 26.37647970373429 27.682470775492828 22.853131320082426 38-39 22.730567949990466 26.534041301543898 27.789584150712933 22.945806597752703 40-41 23.018807391184943 26.366970331652627 27.754888386072746 22.859333891089683 42-43 22.871260410603337 26.34089335648907 27.66067159920822 23.127174633699372 44-45 22.807786098928162 26.483087702574487 27.67137389039233 23.03775230810502 46-47 22.92144840690788 26.60644169707227 27.35361980656812 23.11849008945173 48-49 22.887843754226513 26.565372185452407 27.435692915200878 23.111091145120202 50-51 22.84613600507706 26.68993228036997 27.386228090520525 23.077703624032438 52-53 22.97733230836573 26.688385105772262 27.26435594453069 23.06992664133132 54-55 22.791301677469008 26.505112556520366 27.39886223397918 23.304723532031446 56-57 22.760054383579668 26.58390580955381 27.523051221836365 23.132988585030162 58-59 21.240417152334544 26.32388356294512 29.196812307123526 23.238886977596803 60-61 22.73346664449606 26.46379921479629 27.675180148169304 23.127553992538346 62-63 22.547196944385433 26.59299740131345 27.5017414289368 23.358064225364313 64-65 21.76301690303074 26.479192620037544 28.076976628130613 23.680813848801105 66-67 21.813398109974155 26.08554205988361 28.69926530224567 23.401794527896563 68-69 22.42002830810002 25.39046388884161 29.261141567996397 22.92836623506198 70-71 21.5769432247306 25.710077482532327 29.293257987628973 23.419721305108105 72-73 21.443446783666346 25.845879637908308 29.21503745636203 23.495636122063313 74-75 21.997348687972604 26.00909598995122 28.441201615375345 23.55235370670083 76-77 20.506928174037665 26.394773073114173 28.999331739168944 24.098967013679218 78-79 21.486349015555774 26.580849679965468 27.857869260740465 24.074932043738293 80-81 22.143047108529096 26.56676747359989 27.195481046544774 24.09470437132624 82-83 22.401764013225637 26.57642119733385 26.8979847811498 24.12383000829071 84-85 22.38841980208111 26.458704258727828 26.83893530211774 24.313940637073323 86-87 22.26749900782844 26.435606950997876 27.00090585422627 24.29598818694742 88-89 22.30949786275275 26.46767438419872 26.814812833006783 24.408014920041744 90-91 21.96642241867755 26.492852115317394 27.045035583809085 24.495689882195965 92-93 21.648609926392055 26.345329913259686 27.242580778341676 24.76347938200658 94-95 20.349525201406852 26.270113057578392 28.47087927236859 24.90948246864616 96-97 20.02252832453692 26.177264652323235 28.747116411560196 25.05309061157965 98-99 20.463196050496197 26.19556208486646 28.07832663456923 25.262915230068124 100-101 20.26918318761132 26.320432373936104 28.002051796673218 25.408332641779356 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 157.0 1 118.5 2 80.5 3 92.0 4 109.0 5 123.5 6 170.5 7 276.5 8 435.5 9 567.0 10 655.0 11 804.5 12 999.5 13 1206.5 14 1387.0 15 1584.5 16 1832.5 17 2125.0 18 2498.0 19 2886.0 20 3336.5 21 4004.0 22 4915.0 23 6034.0 24 7531.5 25 9749.0 26 12661.0 27 16155.5 28 20275.5 29 24972.0 30 30070.0 31 36030.5 32 42762.0 33 49208.5 34 55785.0 35 62357.5 36 69695.5 37 76604.0 38 82288.5 39 88404.0 40 94480.5 41 100727.0 42 106543.0 43 112398.5 44 118968.5 45 124416.5 46 128087.0 47 130807.0 48 131567.5 49 130180.5 50 127947.5 51 124750.5 52 121424.0 53 117415.5 54 111826.0 55 104204.5 56 94947.0 57 84913.0 58 74070.5 59 62905.5 60 51854.0 61 41738.0 62 32722.0 63 24849.5 64 18381.0 65 13335.0 66 9607.5 67 6998.5 68 4984.5 69 3458.0 70 2474.0 71 1755.0 72 1266.5 73 988.0 74 692.0 75 439.0 76 279.5 77 166.5 78 102.5 79 60.5 80 28.5 81 19.0 82 13.0 83 5.0 84 1.0 85 1.0 86 0.5 87 0.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020670955651085735 2 0.005764935243214974 3 0.003439850863354791 4 0.0 5 0.06522976451991307 6 9.555141287096641E-5 7 0.0 8 3.1850470956988803E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.3887853217741605E-4 16-17 0.0 18-19 0.0013217945447150353 20-21 0.0 22-23 0.0 24-25 0.0 26-27 9.555141287096641E-5 28-29 0.0020384301412472833 30-31 0.008408524332645046 32-33 2.0702806122042724E-4 34-35 9.395888932311697E-4 36-37 0.027614358319709296 38-39 0.026419965658822212 40-41 0.02882467621607487 42-43 0.029429835164257657 44-45 0.030226096938182376 46-47 0.03169121860220386 48-49 0.0318663961924673 50-51 0.026977348900569516 52-53 0.02261383437946205 54-55 0.01710370290390299 56-57 0.14439411008350875 58-59 0.7544421055581938 60-61 0.29941035223117324 62-63 2.548037676559104E-4 64-65 0.14783396094686355 66-67 1.4115013961653944 68-69 2.6982126471213066 70-71 2.1702910910092172 72-73 1.8585386812822107 74-75 1.5526626834467687 76-77 1.1018988932279847 78-79 0.4207924970482576 80-81 1.9110282574193283E-4 82-83 3.8220565148386565E-4 84-85 3.18504709569888E-5 86-87 0.002962093798999959 88-89 4.7775706435483206E-5 90-91 0.22059636184810447 92-93 0.1729639825319277 94-95 1.287061606136439 96-97 1.2745762215212997 98-99 1.0075896487243408 100-101 0.4030836351961718 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3139671.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.98719159157756 #Duplication Level Percentage of deduplicated Percentage of total 1 71.78985979202123 37.32153195340299 2 14.732033244091221 15.317540695881208 3 5.577722136201871 8.699103280179298 4 2.713538557213459 5.642769954599562 5 1.4806222341349455 3.8486695880361523 6 0.8766456204341806 2.734460629645748 7 0.5876365677678242 2.1384702384334053 8 0.39830707722148634 1.6565493068635755 9 0.2918447658036044 1.365497077934796 >10 1.416611046431054 13.144362439066665 >50 0.09272245093523644 3.2818328060525084 >100 0.04000272202915953 3.5482041208754653 >500 0.0019016839292794899 0.6519869028903313 >1k 4.907571425247052E-4 0.4639554615153787 >5k 6.134464281558815E-5 0.18506554462288577 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5803 0.18482828296340603 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4764 0.15173564363909467 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3215 0.102399264126719 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.185047095698881E-5 0.0 0.0 0.0 0.0 6 6.370094191397762E-5 0.0 0.0 0.0 0.0 7 1.5925235478494402E-4 0.0 0.0 0.0 0.0 8 1.5925235478494402E-4 0.0 0.0 0.0 0.0 9 1.5925235478494402E-4 0.0 0.0 0.0 0.0 10-11 1.5925235478494402E-4 0.0 0.0 0.0 0.0 12-13 2.5480376765591047E-4 0.0 0.0 0.0 1.5925235478494404E-5 14-15 3.3442994504838245E-4 0.0 0.0 1.5925235478494404E-5 3.185047095698881E-5 16-17 3.5035518052687687E-4 0.0 0.0 4.777570643548321E-5 3.185047095698881E-5 18-19 3.9813088696236007E-4 0.0 0.0 6.370094191397762E-5 3.185047095698881E-5 20-21 4.140561224408545E-4 0.0 0.0 1.7517759026343843E-4 3.185047095698881E-5 22-23 4.4590659339784327E-4 0.0 0.0 2.5480376765591047E-4 3.185047095698881E-5 24-25 5.733084772257985E-4 0.0 0.0 5.573832417473041E-4 3.185047095698881E-5 26-27 5.733084772257985E-4 0.0 0.0 0.001433271193064496 3.185047095698881E-5 28-29 6.370094191397761E-4 0.0 0.0 0.005319028649817131 3.185047095698881E-5 30-31 7.007103610537537E-4 0.0 0.0 0.019763217228811553 3.185047095698881E-5 32-33 7.644113029677314E-4 0.0 0.0 0.05403432397853151 3.185047095698881E-5 34-35 8.918131867956865E-4 0.0 0.0 0.10679462911878346 3.185047095698881E-5 36-37 0.0010032898351451476 0.0 0.0 0.19793475176220693 3.185047095698881E-5 38-39 0.0010192150706236419 0.0 0.0 0.3352899077642212 3.185047095698881E-5 40-41 0.0010192150706236419 0.0 0.0 0.4888728787188212 3.185047095698881E-5 42-43 0.0010510655415806305 0.0 0.0 0.6463893828366094 3.185047095698881E-5 44-45 0.0011147664834946082 0.0 0.0 0.8325872360511659 3.185047095698881E-5 46-47 0.0011625421899300915 0.0 0.0 1.048183073959023 6.370094191397762E-5 48-49 0.0012103178963655747 0.0 0.0 1.2568673596692137 6.370094191397762E-5 50-51 0.0012103178963655747 0.0 0.0 1.4589267474203507 6.370094191397762E-5 52-53 0.0012103178963655747 0.0 0.0 1.6543771624479127 6.370094191397762E-5 54-55 0.0012103178963655747 0.0 0.0 1.8646858221769096 6.370094191397762E-5 56-57 0.0012103178963655747 0.0 0.0 2.047332347879762 6.370094191397762E-5 58-59 0.0012103178963655747 1.5925235478494404E-5 0.0 2.1723613716214216 6.370094191397762E-5 60-61 0.0012103178963655747 3.185047095698881E-5 0.0 2.265412522522264 6.370094191397762E-5 62-63 0.0012421683673225635 3.185047095698881E-5 0.0 2.345675709333876 6.370094191397762E-5 64-65 0.0012740188382795521 4.777570643548321E-5 0.0 2.431480878092004 6.370094191397762E-5 66-67 0.0012740188382795521 6.370094191397762E-5 0.0 2.500739727187976 6.370094191397762E-5 68-69 0.0012740188382795521 6.370094191397762E-5 0.0 2.5806207083481043 6.370094191397762E-5 70-71 0.0012740188382795521 6.370094191397762E-5 0.0 2.727276201869559 6.370094191397762E-5 72-73 0.0012740188382795521 6.370094191397762E-5 0.0 2.916229120821895 6.370094191397762E-5 74-75 0.0012740188382795521 6.370094191397762E-5 0.0 3.139023165166032 6.370094191397762E-5 76-77 0.0012740188382795521 6.370094191397762E-5 0.0 3.4633565109210487 6.370094191397762E-5 78-79 0.0012740188382795521 6.370094191397762E-5 0.0 3.924662807026596 6.370094191397762E-5 80-81 0.0014651216640214852 6.370094191397762E-5 0.0 4.439461969104406 6.370094191397762E-5 82-83 0.0015288226059354626 6.370094191397762E-5 0.0 4.923748380005421 6.370094191397762E-5 84-85 0.0015606730768924514 6.370094191397762E-5 0.0 5.314267004409061 6.370094191397762E-5 86-87 0.0015606730768924514 6.370094191397762E-5 0.0 5.665641400006562 6.370094191397762E-5 88-89 0.0015606730768924514 6.370094191397762E-5 0.0 6.023943273037207 6.370094191397762E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4900 0.0 40.718372 1 GTATCAA 7915 0.0 36.05376 1 ATCAACG 9505 0.0 29.220768 3 TCAACGC 9620 0.0 28.918627 4 CAACGCA 9805 0.0 28.324905 5 TATCAAC 10010 0.0 27.983917 2 AACGCAG 10270 0.0 27.253744 6 ACGCAGA 11705 0.0 23.791742 7 CGCAGAG 12025 0.0 23.041058 8 GTACATG 9070 0.0 22.465837 1 TACATGG 9135 0.0 21.423758 2 GCAGAGT 13240 0.0 20.463976 9 ACATGGG 9230 0.0 20.384481 3 GAGTACT 7135 0.0 20.0107 12-13 AGAGTAC 11660 0.0 18.77143 10-11 GTACTTT 7600 0.0 18.476503 14-15 CAGAGTA 12685 0.0 17.886114 10-11 GGACCGA 720 0.0 17.670198 6 GTATAGG 885 0.0 17.588013 1 AGTACTT 7770 0.0 17.43534 12-13 >>END_MODULE