FastQCFastQC Report
Fri 27 May 2016
SRR939742_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939742_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3270625
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52800.16143703420600036No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40500.12382954328301166No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA80950.037.303621
GGTATCA49600.037.078991
ATCAACG95700.030.9170573
TCAACGC96850.030.5989824
TATCAAC99800.029.7869452
CAACGCA99950.029.368915
AACGCAG105150.028.2301226
GTACATG89450.024.7144151
ACGCAGA118050.024.7069057
CGCAGAG119150.024.2419198
TACATGG90150.023.479362
ACATGGG94650.021.3701343
GCAGAGT129900.021.2941769
GAGTACT68000.019.95704812-13
AGAGTAC109400.019.843610-11
GTACTTT73650.018.55962414-15
CATGGGG61900.018.4663754
AGTACTT72500.018.26416412-13
CAGAGTA123050.017.90993510-11
TAAGGTA8750.017.740754
Produced by FastQC (version 0.11.5)