FastQCFastQC Report
Fri 27 May 2016
SRR939742_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939742_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3270625
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60280.1843072807185171No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49610.1516835467227212No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51500.040.177691
GTATCAA80050.034.800261
ATCAACG96250.028.1874163
TCAACGC96550.028.001584
CAACGCA98250.027.4205255
TATCAAC100150.027.0980382
AACGCAG102800.026.299156
GTACATG88750.024.4373171
TACATGG90100.023.5383222
ACGCAGA116700.023.1666897
CGCAGAG119400.022.5236458
ACATGGG93150.021.4367623
GCAGAGT133750.019.5751559
GAGTACT72500.019.56109412-13
TGCACCG8250.018.97235
GCACCGT7500.018.9722986
GTACTTT74550.018.80051814-15
AGAGTAC116050.018.7597710-11
GTATAGA10300.017.9693781
CAGAGTA129000.017.61188110-11
Produced by FastQC (version 0.11.5)