##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939742_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3270625 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.407305943053696 31.0 31.0 34.0 30.0 34.0 2 31.643541830689852 31.0 31.0 34.0 30.0 34.0 3 31.69425599082744 31.0 31.0 34.0 30.0 34.0 4 35.49744712402064 37.0 35.0 37.0 33.0 37.0 5 35.223476246894705 37.0 35.0 37.0 32.0 37.0 6 35.23980064972291 37.0 35.0 37.0 32.0 37.0 7 35.152086833556275 37.0 35.0 37.0 32.0 37.0 8 35.10893453086184 37.0 35.0 37.0 32.0 37.0 9 36.709875329638834 39.0 35.0 39.0 32.0 39.0 10-11 36.571286374928334 38.5 35.0 39.0 32.0 39.0 12-13 36.49036009936938 38.0 35.0 39.0 32.0 39.0 14-15 37.554085228358495 39.0 36.0 41.0 32.0 41.0 16-17 37.46059193579209 39.0 36.0 41.0 32.0 41.0 18-19 37.41377015096503 39.0 36.0 41.0 32.0 41.0 20-21 37.31580875214982 39.0 36.0 40.5 31.0 41.0 22-23 37.18501586088286 39.0 36.0 40.0 31.0 41.0 24-25 37.0334903879228 39.0 36.0 40.0 31.0 41.0 26-27 36.73363042231989 39.0 35.5 40.0 30.0 41.0 28-29 36.50927712593159 38.0 35.0 40.0 30.0 41.0 30-31 36.26215585706096 38.0 35.0 40.0 30.0 41.0 32-33 36.28263726351997 38.0 35.0 40.0 30.0 41.0 34-35 36.14351461876552 38.0 35.0 40.0 30.0 41.0 36-37 36.225589145805465 38.0 35.0 40.0 30.0 41.0 38-39 36.14359870055418 38.0 35.0 40.0 30.0 41.0 40-41 36.076641467609406 38.0 35.0 40.0 29.5 41.0 42-43 35.87984513663291 38.0 35.0 40.0 29.0 41.0 44-45 35.66390949742022 38.0 34.0 40.0 28.0 41.0 46-47 35.42074710491114 38.0 34.0 40.0 27.0 41.0 48-49 35.14837531052933 38.0 34.0 40.0 26.5 41.0 50-51 34.93898643225683 38.0 33.5 40.0 26.0 41.0 52-53 34.694697496655834 38.0 33.0 40.0 26.0 41.0 54-55 34.427541524937894 37.5 33.0 40.0 25.0 41.0 56-57 34.04953013567743 37.0 32.5 40.0 24.5 41.0 58-59 33.67527169883432 37.0 32.0 39.5 23.0 41.0 60-61 33.35865774890121 36.0 32.0 39.0 23.0 41.0 62-63 33.057663902159376 36.0 31.0 39.0 22.0 40.0 64-65 32.612686566023314 35.5 31.0 39.0 21.5 40.0 66-67 31.655683088094783 35.0 30.0 38.0 16.5 40.0 68-69 29.654484999044527 34.0 26.5 37.0 9.5 39.5 70-71 28.9868311102618 33.5 25.5 36.5 9.0 39.0 72-73 29.599536327154595 34.0 27.0 36.0 10.0 39.0 74-75 29.730729600611504 34.0 28.0 36.0 11.5 39.0 76-77 29.30855371679725 32.5 28.0 35.0 12.0 37.0 78-79 29.860119549015863 34.0 29.0 35.0 12.5 37.0 80-81 29.67723386202943 34.0 29.0 35.0 9.5 37.0 82-83 29.232417810051594 33.5 29.0 35.0 7.0 36.5 84-85 28.87510934454424 33.0 29.0 35.0 6.0 36.0 86-87 28.48504154404739 33.0 27.5 35.0 2.0 36.0 88-89 28.033412268297344 33.0 27.0 35.0 2.0 35.0 90-91 27.539630345881903 33.0 26.0 35.0 2.0 35.0 92-93 27.041060806420788 32.0 25.5 34.5 2.0 35.0 94-95 26.199306248805655 32.0 24.0 34.0 2.0 35.0 96-97 25.676222893177908 32.0 23.5 34.0 2.0 35.0 98-99 25.001156048155934 32.0 20.0 34.0 2.0 35.0 100-101 23.49233296388305 30.0 8.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.16038498770924292 1101 2 0.22706099874754315 1101 3 0.24174121904701806 1101 4 0.14470204832571198 1101 5 0.23930712558227896 1101 6 0.14569789894204632 1101 7 0.10534978015861896 1101 8 0.099778065346392 1101 9 0.31430052854134516 1101 10-11 0.22961481127303074 1101 12-13 0.22284444490298938 1101 14-15 0.2699963854608285 1101 16-17 0.28843832403057235 1101 18-19 0.15220228617673115 1101 20-21 0.24005632276507782 1101 22-23 0.16806292988452043 1101 24-25 0.14688384817586098 1101 26-27 0.1074282392336201 1101 28-29 0.04824972049848242 1101 30-31 0.02555731295208119 1101 32-33 -0.0545511473015452 1101 34-35 -0.026307956810342148 1101 36-37 0.22344875787360508 1101 38-39 0.21186817416267445 1101 40-41 0.23070165584535118 1101 42-43 -0.015375955335436231 1101 44-45 -0.11781668977065607 1101 46-47 -0.08837661826360232 1101 48-49 -0.01822214807842215 1101 50-51 0.01866573709746433 1101 52-53 -0.08244258570384488 1101 54-55 -0.20312923472615552 1101 56-57 -0.29554519500810983 1101 58-59 -0.09247697648100228 1101 60-61 -0.1280900829006768 1101 62-63 -0.2457315530332238 1101 64-65 -0.4244969930328537 1101 66-67 -0.6895640675048647 1101 68-69 -9.1694835068656 1101 70-71 -7.059252610415395 1101 72-73 -2.054670497522256 1101 74-75 -0.902714758534291 1101 76-77 -0.7667392124477317 1101 78-79 -0.9663485853570215 1101 80-81 -0.8966036772697628 1101 82-83 -0.8551580870630531 1101 84-85 -0.685573894053551 1101 86-87 -0.6894859030652896 1101 88-89 -0.6270143498272525 1101 90-91 -0.8757610285239039 1101 92-93 -1.0415207381927445 1101 94-95 -0.6786467626128605 1101 96-97 -1.2381130200267378 1101 98-99 -1.0765315447646735 1101 100-101 -1.0522460360009056 1102 1 0.17918547189125889 1102 2 0.21136078695510108 1102 3 0.2799898130893581 1102 4 0.22134502657074506 1102 5 0.32744273225034704 1102 6 0.2002625938215843 1102 7 0.33692629247119754 1102 8 0.17864607913397634 1102 9 0.27174889328650664 1102 10-11 0.33919062141259104 1102 12-13 0.3278008392643841 1102 14-15 0.4340590365507566 1102 16-17 0.3976521381937346 1102 18-19 0.3483576161707731 1102 20-21 0.2854213609953291 1102 22-23 0.31602667772308024 1102 24-25 0.22911371363731803 1102 26-27 0.43938648374157196 1102 28-29 0.1518814823229846 1102 30-31 0.15861538057789204 1102 32-33 0.16308467681204064 1102 34-35 0.037831779587165215 1102 36-37 0.059272425170540544 1102 38-39 0.4187047409134905 1102 40-41 0.3010451602738513 1102 42-43 0.2362406294358408 1102 44-45 0.22803116679166635 1102 46-47 0.25432298904514283 1102 48-49 0.2744362264009581 1102 50-51 0.2582133512216558 1102 52-53 0.15305067348477763 1102 54-55 -0.05531722766673397 1102 56-57 -0.026005098498991686 1102 58-59 -0.06983388021536996 1102 60-61 0.0648136058383173 1102 62-63 0.20303262741532535 1102 64-65 0.24612348790608252 1102 66-67 0.4262944803358799 1102 68-69 -4.560227170572329 1102 70-71 0.33739914995120657 1102 72-73 0.7500506490429721 1102 74-75 0.591899683518335 1102 76-77 0.3987256771658103 1102 78-79 0.23119522235468537 1102 80-81 0.2797275381391664 1102 82-83 0.24702060803566894 1102 84-85 0.2817699778256575 1102 86-87 0.13785218183714676 1102 88-89 0.06950359599930422 1102 90-91 -0.05205133342865409 1102 92-93 -0.08586499735806186 1102 94-95 0.13520486465823112 1102 96-97 -0.32758317692967864 1102 98-99 -0.005884930691561152 1102 100-101 6.37485099243662E-4 1103 1 0.18782726488652202 1103 2 0.1701108116298755 1103 3 0.19597114571705632 1103 4 0.15172668223183905 1103 5 0.2258716175527482 1103 6 0.16419519558222362 1103 7 0.10562033851694963 1103 8 0.19295095139610652 1103 9 0.29832918450131274 1103 10-11 0.2156353450463726 1103 12-13 0.2804846176057012 1103 14-15 0.28026685486846503 1103 16-17 0.27098459198069236 1103 18-19 0.29823671602502344 1103 20-21 0.17302308040854086 1103 22-23 0.1727337060732097 1103 24-25 0.158538612745609 1103 26-27 0.10852949823185298 1103 28-29 0.016661846849977735 1103 30-31 0.08618561123143564 1103 32-33 0.09197547638216719 1103 34-35 -0.024286058138031308 1103 36-37 -0.016583452160723766 1103 38-39 0.1678336276901362 1103 40-41 0.1091752421841008 1103 42-43 0.14339176623703764 1103 44-45 0.12026787071162204 1103 46-47 0.09646440461006733 1103 48-49 0.06441304585632679 1103 50-51 0.06635442746158304 1103 52-53 0.1606183954321878 1103 54-55 0.11577765514745408 1103 56-57 0.2228390601262049 1103 58-59 0.12656657624921053 1103 60-61 0.114032221337375 1103 62-63 -0.030664343371981317 1103 64-65 -0.09942804389140036 1103 66-67 0.24124267080664197 1103 68-69 -4.072298944554898 1103 70-71 0.25329677575070164 1103 72-73 0.5958020777436204 1103 74-75 0.5183308766304897 1103 76-77 0.31867371396583266 1103 78-79 0.12986036080952346 1103 80-81 0.23398088197708233 1103 82-83 0.06293080235295179 1103 84-85 0.1119546850629014 1103 86-87 0.10585930716226244 1103 88-89 0.0637819219526925 1103 90-91 -0.05563810854938467 1103 92-93 -0.0957055470945356 1103 94-95 -0.042817695206519346 1103 96-97 -0.7451780523726654 1103 98-99 -0.46752284093934904 1103 100-101 -0.4001990334663077 1104 1 0.19964557177175024 1104 2 0.2544307324241224 1104 3 0.17062764996306612 1104 4 0.26129261654373437 1104 5 0.312919073282778 1104 6 0.1878018022050867 1104 7 0.12466905618691015 1104 8 0.18793918383901342 1104 9 0.26383555839660744 1104 10-11 0.26882162040015345 1104 12-13 0.18306067383680613 1104 14-15 0.2958571667902916 1104 16-17 0.3402871242068102 1104 18-19 0.33836590581400117 1104 20-21 0.26558656315286555 1104 22-23 0.16679397157579956 1104 24-25 0.1465596528392581 1104 26-27 0.27668602797573527 1104 28-29 0.19756530631335778 1104 30-31 0.19348293139638173 1104 32-33 0.18687992657334007 1104 34-35 0.187895671378854 1104 36-37 0.1754916847530339 1104 38-39 0.32264730707967004 1104 40-41 0.25137653209791466 1104 42-43 0.118608485891734 1104 44-45 0.18951731652223458 1104 46-47 0.13228748673110147 1104 48-49 0.24259987462061616 1104 50-51 0.24506765210345094 1104 52-53 0.08933448961003165 1104 54-55 0.14780762064579278 1104 56-57 0.17698280944878064 1104 58-59 0.11985567445571377 1104 60-61 0.22541121046379686 1104 62-63 0.14811135516735874 1104 64-65 0.2018122761389307 1104 66-67 0.36624146150219516 1104 68-69 -3.899929362095339 1104 70-71 0.9102372322233627 1104 72-73 1.0589739201513275 1104 74-75 0.6730120654448051 1104 76-77 0.5241923809532274 1104 78-79 0.3745780779969152 1104 80-81 0.32525340148049864 1104 82-83 0.23239661937461165 1104 84-85 0.10367074464233639 1104 86-87 0.2216337285121348 1104 88-89 0.1565175358620401 1104 90-91 0.06696703821754468 1104 92-93 -0.008866091729181846 1104 94-95 0.34444180283457015 1104 96-97 -0.022658819512777484 1104 98-99 0.06594713969701971 1104 100-101 0.1227515152463603 1105 1 0.20947079096832866 1105 2 0.1899575877093227 1105 3 0.2027923678999599 1105 4 0.11945052901449316 1105 5 0.2415308539977019 1105 6 0.21313141913577027 1105 7 0.20669099898429977 1105 8 0.2851130723485582 1105 9 0.36276098974640547 1105 10-11 0.21319022302247959 1105 12-13 0.1628034106539502 1105 14-15 0.157659072678058 1105 16-17 0.19067400139746127 1105 18-19 0.19632234173732144 1105 20-21 0.2714845120136289 1105 22-23 0.263244566181271 1105 24-25 0.24070562854515742 1105 26-27 0.2781023313783919 1105 28-29 0.41576692413389793 1105 30-31 0.33213959473690835 1105 32-33 0.3116172113855882 1105 34-35 0.3398026146268478 1105 36-37 0.26023285051589795 1105 38-39 0.4846204051094034 1105 40-41 0.3534916095910816 1105 42-43 0.28049154261938014 1105 44-45 0.18454247368855903 1105 46-47 0.25164470225458047 1105 48-49 0.3107757141603642 1105 50-51 0.2816478038827199 1105 52-53 0.22588253017651283 1105 54-55 0.3901187105027901 1105 56-57 0.3070893244931554 1105 58-59 0.3099206438424815 1105 60-61 0.3223632839712778 1105 62-63 0.35306922899098936 1105 64-65 0.4016434385485965 1105 66-67 1.0131589068623903 1105 68-69 2.2141558093444367 1105 70-71 2.4462843216321843 1105 72-73 1.532854919487992 1105 74-75 1.2110999421832176 1105 76-77 0.7346843848146953 1105 78-79 0.6221431062474743 1105 80-81 0.4920864158668081 1105 82-83 0.4714408906287062 1105 84-85 0.4166076256083855 1105 86-87 0.30163786115620894 1105 88-89 0.4815683414028662 1105 90-91 0.44757572229111275 1105 92-93 0.5717146218948237 1105 94-95 0.84250039382216 1105 96-97 0.5133178363256619 1105 98-99 0.6803281321324128 1105 100-101 0.5052983606749173 1106 1 0.38932811948975754 1106 2 0.3494792911389837 1106 3 0.35952589615057917 1106 4 0.22072770248423268 1106 5 0.3483578229130444 1106 6 0.36433690340493285 1106 7 0.33659228358951765 1106 8 0.3828276278845735 1106 9 0.36903501831695706 1106 10-11 0.4173241794144147 1106 12-13 0.4517806749360531 1106 14-15 0.6231139411088904 1106 16-17 0.5685201351653362 1106 18-19 0.4870054192794697 1106 20-21 0.564614719903247 1106 22-23 0.44456173515570896 1106 24-25 0.4535016976930635 1106 26-27 0.5926141832074165 1106 28-29 0.525730680756773 1106 30-31 0.5809750757252203 1106 32-33 0.6239733571711454 1106 34-35 0.6943471752200878 1106 36-37 0.6191246677963491 1106 38-39 0.6768210098001006 1106 40-41 0.6022266260043736 1106 42-43 0.66750872808867 1106 44-45 0.8939547853797833 1106 46-47 0.7838481527618413 1106 48-49 0.7454769124847758 1106 50-51 0.8423292681912926 1106 52-53 0.7919295369031616 1106 54-55 0.8174545005431568 1106 56-57 0.7205921802791337 1106 58-59 0.8615627434388173 1106 60-61 0.9769122854884458 1106 62-63 0.9566709089290697 1106 64-65 0.9418909883367874 1106 66-67 1.506528290226509 1106 68-69 2.8790266056474145 1106 70-71 3.215732351040163 1106 72-73 2.1078678344198316 1106 74-75 1.6001358447874132 1106 76-77 1.1935576680350728 1106 78-79 1.0548810989861117 1106 80-81 1.0276533677797595 1106 82-83 1.1726466014756127 1106 84-85 1.2250641870281775 1106 86-87 1.18045066624315 1106 88-89 1.4671353839469212 1106 90-91 1.2397835774698613 1106 92-93 1.1311295618879704 1106 94-95 1.4642886995099857 1106 96-97 1.3575802532395969 1106 98-99 1.654517255675291 1106 100-101 1.6932572939106123 1107 1 0.11595339588961551 1107 2 0.04641277881912487 1107 3 0.039274735911764225 1107 4 0.10529467980398977 1107 5 0.0556490515226713 1107 6 0.052168602078964454 1107 7 0.07758658451454181 1107 8 0.12949764074178916 1107 9 0.02326538695561453 1107 10-11 0.06153274505928863 1107 12-13 0.1043654564011689 1107 14-15 0.14021022397353278 1107 16-17 0.0961047282188261 1107 18-19 0.024671353401721774 1107 20-21 0.1343907060120415 1107 22-23 0.07716452123616335 1107 24-25 0.15141643314052544 1107 26-27 -0.0038791372234854293 1107 28-29 0.1592638434645295 1107 30-31 0.19214208228646612 1107 32-33 0.12143884392882853 1107 34-35 0.19302301828676605 1107 36-37 -0.020830128485457067 1107 38-39 0.1245495945574504 1107 40-41 0.035767438224304726 1107 42-43 0.03436293969151194 1107 44-45 -0.0197560796406151 1107 46-47 0.09787927987035516 1107 48-49 0.0403558090256908 1107 50-51 0.054538040335302185 1107 52-53 0.1047663226911908 1107 54-55 0.1843224661949634 1107 56-57 0.2181820187123904 1107 58-59 0.1782033716182596 1107 60-61 0.3201296929142714 1107 62-63 0.2967235662113339 1107 64-65 0.31491586922295767 1107 66-67 0.7744654541943667 1107 68-69 2.135438064993462 1107 70-71 2.2615288122175734 1107 72-73 1.2355197281074375 1107 74-75 0.6107581156449555 1107 76-77 0.256299117538493 1107 78-79 0.3336393080808122 1107 80-81 0.3142628778944463 1107 82-83 0.3314879679271918 1107 84-85 0.38363367549813887 1107 86-87 0.4613153173575668 1107 88-89 0.40086762238259155 1107 90-91 0.40083576598398807 1107 92-93 0.3204250305129115 1107 94-95 0.6581936586548096 1107 96-97 0.4053410940679463 1107 98-99 0.6874728990557877 1107 100-101 0.7787219395987748 1108 1 -0.14636902048988532 1108 2 -0.14023466582447597 1108 3 -0.09359384481464161 1108 4 -0.055269238120331465 1108 5 -0.029170474071953834 1108 6 -0.05681433576576467 1108 7 -0.07245211007207786 1108 8 0.03342728131709549 1108 9 -0.13367326229013798 1108 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-0.6094505000007402 2316 54-55 -0.6460214137156512 2316 56-57 -0.5489907729323349 2316 58-59 -0.7321206826589801 2316 60-61 -0.6907574717845577 2316 62-63 -0.760085620018863 2316 64-65 -0.4909033605812212 2316 66-67 0.1919321258086093 2316 68-69 1.4403988319144538 2316 70-71 1.7197414012002703 2316 72-73 0.7673405764749148 2316 74-75 0.21058306578637342 2316 76-77 -0.17164728021577957 2316 78-79 -0.3500640919622988 2316 80-81 -0.42708726187450097 2316 82-83 -0.30325624841841403 2316 84-85 -0.32484896853220135 2316 86-87 -0.3763896977148633 2316 88-89 -0.3740126345613852 2316 90-91 -0.35653199539219216 2316 92-93 -0.3089818437869205 2316 94-95 -0.08899402871980655 2316 96-97 -0.30814551980104454 2316 98-99 -0.32809634871279414 2316 100-101 -0.4347879396450942 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 15.0 4 70.0 5 141.0 6 291.0 7 753.0 8 1736.0 9 2923.0 10 4634.0 11 7109.0 12 9842.0 13 12831.0 14 15591.0 15 19168.0 16 22862.0 17 26531.0 18 26174.0 19 24351.0 20 24898.0 21 26094.0 22 28603.0 23 32186.0 24 39504.0 25 47680.0 26 59161.0 27 73778.0 28 90375.0 29 108818.0 30 130419.0 31 155281.0 32 186424.0 33 225097.0 34 278939.0 35 351763.0 36 445569.0 37 489567.0 38 279369.0 39 22073.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.70759328256078 19.538502518596264 14.666576143383987 24.08732805545897 2 16.013003734139083 20.50666242587535 38.86101112290241 24.61932271708315 3 19.395136830158403 23.881834169870945 29.70411762403749 27.018911375933158 4 13.64246894706669 16.925602904643608 36.41291802025607 33.019010128033635 5 14.152921842155553 36.94752532008408 34.39908274412383 14.500470093636537 6 33.01568752629468 36.059540931638736 16.969452923968024 13.955318618098564 7 28.959785973628893 31.793036499140072 20.724876743741643 18.522300783489396 8 26.199212688706286 35.595520733804705 19.417359067456527 18.787907510032486 9 25.84084081788649 16.53078540034397 19.84623733995796 37.78213644181158 10-11 25.05608255302886 26.30873304032104 27.534645518822852 21.10053888782725 12-13 25.79172176571756 23.24739155360214 27.841345308618383 23.119541372061914 14-15 22.578085228358493 25.108320275176766 25.5639518440665 26.749642652398244 16-17 22.756222002555486 28.06417516482394 26.271849510687755 22.907753321932812 18-19 22.662992547296007 27.04522835849417 27.93051786738009 22.361261226829736 20-21 23.17826103573476 26.295371679724823 28.07409898719664 22.45226829734378 22-23 22.935922033250524 26.147143130135674 27.86698261035735 23.049952226256448 24-25 22.81098346982105 26.352371902120208 27.98985759292416 22.84678703513458 26-27 22.734324526700426 26.625059660252255 27.84070705004551 22.79990876300181 28-29 22.681867845687528 26.777784113640717 27.816760321082505 22.723587719589247 30-31 22.83183213391276 26.506639287447253 27.786906222606383 22.874622356033605 32-33 22.691184851976058 26.44784427827474 27.80426668036466 23.056704189384543 34-35 23.029663517487638 26.598220078875222 27.64375403992439 22.72836236371275 36-37 23.09376460581181 26.38742777631709 27.666036037387205 22.852771580483896 38-39 22.72491887990921 26.57308092299085 27.765703876319176 22.936296320780762 40-41 23.02834361273932 26.352825943894338 27.744785050733846 22.874045392632496 42-43 22.821845130991477 26.366231301224925 27.623934983060277 23.18798858472332 44-45 22.851463505410095 26.481992051968277 27.629403432393985 23.037141010227643 46-47 22.94018901532077 26.612980725089212 27.351612567560124 23.095217692029895 48-49 22.892025710758254 26.58438171208774 27.403405616531177 23.120186960622828 50-51 22.828571935737198 26.722286866426437 27.3801224070822 23.069018790754157 52-53 23.01272891391154 26.680782705812156 27.20106176277099 23.105426617505316 54-55 22.803742263660155 26.531136731402725 27.367131068871593 23.297989936065534 56-57 22.769071298449777 26.696514539980203 27.322763214076417 23.2116509474936 58-59 22.835440498704724 26.492717539233272 27.28537194683488 23.386470015227122 60-61 22.765339148497212 26.617569241725647 27.369136261331334 23.24795534844581 62-63 22.624480114598374 26.594940763066795 27.405170838872024 23.37540828346281 64-65 22.446198975157422 26.513647113466064 27.342859331282344 23.697294580094166 66-67 22.241373713169878 26.579523299381293 27.491383360376137 23.687719627072696 68-69 22.10817602569026 26.336907037873754 27.932936189082714 23.621980747353273 70-71 22.122307210874453 26.33811074048506 27.64821728767126 23.891364760969225 72-73 22.178453286800778 26.402042375586582 27.477316298627734 23.942188038984902 74-75 22.389145909568516 26.633532382694096 26.91049079183252 24.06683091590487 76-77 22.317009799258795 26.541435734094136 27.05348133816053 24.088073128486545 78-79 22.404589716709502 26.5929997524799 26.974446722254473 24.027963808556123 80-81 22.41556623294466 26.628888298097593 26.880327918726003 24.07521755023174 82-83 22.40087824219476 26.57800162904693 26.867609972898137 24.153510155860168 84-85 22.37516170525935 26.460162948146387 26.824330377412657 24.340344969181597 86-87 22.319406626303877 26.44068579137106 26.938914472985196 24.30099310933987 88-89 22.316380510029383 26.470201098233648 26.80097981557294 24.41243857616403 90-91 22.288754265457655 26.46070474127948 26.801467435148457 24.44907355811441 92-93 22.19772407788616 26.212822490458386 26.908716214511813 24.68073721714364 94-95 21.838785355239647 26.17746246005253 27.17481992560633 24.80893225910149 96-97 22.025028570135436 26.014480632093413 27.12393766037731 24.836553137393835 98-99 22.02469418960245 26.028211009174314 26.922874617737 25.02422018348624 100-101 21.808444846107708 25.995855719851185 27.04782648513947 25.147872948901632 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 247.0 1 172.5 2 126.0 3 159.0 4 148.0 5 162.0 6 279.5 7 559.5 8 894.5 9 1135.0 10 1172.0 11 1154.0 12 1299.5 13 1505.5 14 1688.5 15 1868.0 16 2079.5 17 2308.0 18 2602.0 19 2953.0 20 3414.0 21 4043.5 22 4918.5 23 6137.5 24 7701.0 25 9855.5 26 13000.0 27 17172.5 28 21274.0 29 25817.0 30 31157.0 31 37142.0 32 43947.5 33 50116.0 34 55537.5 35 61267.0 36 69340.0 37 77197.5 38 82768.5 39 87508.5 40 92320.0 41 98174.5 42 103416.0 43 109101.0 44 116771.5 45 123998.5 46 128511.5 47 131762.5 48 133380.5 49 132197.5 50 129329.0 51 125578.0 52 123252.5 53 121764.5 54 119629.0 55 113473.5 56 103932.0 57 94813.5 58 86005.5 59 75089.0 60 62687.0 61 51647.5 62 41629.5 63 32303.5 64 24181.0 65 17635.0 66 12504.0 67 9502.5 68 7090.5 69 4815.0 70 3459.5 71 2515.5 72 1841.0 73 1469.0 74 1105.5 75 759.5 76 513.0 77 318.0 78 188.0 79 98.0 80 55.5 81 37.0 82 23.0 83 15.0 84 8.5 85 6.5 86 4.0 87 1.5 88 3.0 89 2.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02287024651251672 2 0.0243072807185171 3 3.057519587234856E-5 4 0.0 5 0.0 6 3.057519587234856E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.2805274221287975E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.057519587234856E-5 26-27 6.726543091916682E-4 28-29 4.5862793808522836E-5 30-31 5.503535257022741E-4 32-33 0.0022319892986814444 34-35 0.0043722530097458434 36-37 0.10756353907892223 38-39 0.09927766099751577 40-41 0.10930632524364609 42-43 0.11005541754251862 44-45 0.10398624116185744 46-47 0.09677049493598319 48-49 0.09781005159564303 50-51 0.09683164532772788 52-53 0.07567360978406268 54-55 0.06488056564112364 56-57 0.035375501624307276 58-59 0.014477355245557042 60-61 0.009921651060577106 62-63 0.0022167017007452706 64-65 0.005243646092107777 66-67 0.166053888782725 68-69 0.43702656220141406 70-71 0.41348366137970566 72-73 0.19528377603669023 74-75 0.030590483470284733 76-77 0.033388113892604626 78-79 0.00561054844257596 80-81 0.0017886489585323905 82-83 3.057519587234856E-5 84-85 4.2805274221287975E-4 86-87 0.0011771450410854195 88-89 2.904643607873113E-4 90-91 6.267915153831455E-4 92-93 0.0025224536594687557 94-95 0.08376074909229887 96-97 0.01840626791515383 98-99 0.019109497420217846 100-101 0.010227403019300592 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3270625.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.861633313196606 #Duplication Level Percentage of deduplicated Percentage of total 1 71.8205782243502 37.24732492213003 2 14.66086508240351 15.206728179157174 3 5.602819959974405 8.717141828521546 4 2.681324509646133 5.562314740518178 5 1.4938990980766464 3.873802361568309 6 0.8851712002123396 2.7543854522888704 7 0.5743532211560712 2.0850827303494643 8 0.41827458148061114 1.7353922375182584 9 0.286597618815511 1.3377078553900796 >10 1.439422139561861 13.235906060515873 >50 0.09359854431383698 3.3178525570971376 >100 0.04079790859271581 3.638585113947934 >500 0.0016497825558465893 0.5720936230641916 >1k 5.892080548426351E-4 0.5314829812418941 >5k 5.8920805484263515E-5 0.18419935669097556 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6028 0.1843072807185171 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4961 0.1516835467227212 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.057519587234856E-5 0.0 0.0 0.0 0.0 6 3.057519587234856E-5 0.0 0.0 0.0 0.0 7 9.172558761704567E-5 0.0 0.0 3.057519587234856E-5 0.0 8 9.172558761704567E-5 0.0 0.0 3.057519587234856E-5 0.0 9 9.172558761704567E-5 0.0 0.0 6.115039174469712E-5 0.0 10-11 9.172558761704567E-5 0.0 0.0 6.115039174469712E-5 0.0 12-13 9.172558761704567E-5 0.0 0.0 7.64379896808714E-5 0.0 14-15 9.172558761704567E-5 0.0 0.0 9.172558761704567E-5 0.0 16-17 1.2230078348939423E-4 0.0 0.0 9.172558761704567E-5 0.0 18-19 1.6816357729791708E-4 0.0 0.0 9.172558761704567E-5 0.0 20-21 1.8345117523409135E-4 0.0 0.0 1.2230078348939423E-4 0.0 22-23 2.4460156697878846E-4 0.0 0.0 3.2103955665965984E-4 0.0 24-25 3.057519587234856E-4 0.0 0.0 4.739155360214026E-4 0.0 26-27 3.057519587234856E-4 0.0 0.0 0.0013605962163195109 0.0 28-29 4.280527422128798E-4 0.0 0.0 0.005274221287980127 0.0 30-31 4.280527422128798E-4 0.0 0.0 0.018696732275941142 0.0 32-33 4.4334034014905407E-4 0.0 0.0 0.05356774316835467 0.0 34-35 5.197783298299255E-4 0.0 0.0 0.10945920122300784 0.0 36-37 5.809287215746226E-4 0.0 0.0 0.20176571756162812 0.0 38-39 6.420791133193197E-4 0.0 0.0 0.3427326581310911 0.0 40-41 6.420791133193197E-4 0.0 0.0 0.4970609592967705 0.0 42-43 6.726543091916682E-4 0.0 0.0 0.6501509650296198 0.0 44-45 7.643798968087139E-4 0.0 0.0 0.8365526466653928 0.0 46-47 8.561054844257596E-4 0.0 0.0 1.056831645327728 0.0 48-49 8.866806802981081E-4 0.0 0.0 1.2937435505446206 0.0 50-51 8.866806802981081E-4 0.0 0.0 1.5422281673991973 0.0 52-53 8.866806802981081E-4 0.0 0.0 1.7818918402446016 0.0 54-55 8.866806802981081E-4 0.0 0.0 2.0373170265622016 0.0 56-57 9.478310720428053E-4 0.0 0.0 2.331985476781961 0.0 58-59 9.478310720428053E-4 0.0 0.0 2.636774316835467 0.0 60-61 9.478310720428053E-4 1.528759793617428E-5 0.0 2.939438180775846 0.0 62-63 9.784062679151538E-4 3.057519587234856E-5 0.0 3.2322415440473917 0.0 64-65 0.0010089814637875025 3.057519587234856E-5 0.0 3.5455608637492837 0.0 66-67 0.0010089814637875025 3.057519587234856E-5 0.0 3.876338620294286 0.0 68-69 0.0010089814637875025 3.057519587234856E-5 0.0 4.239770686030957 0.0 70-71 0.0010701318555321995 3.057519587234856E-5 0.0 4.660791133193197 0.0 72-73 0.0011312822472768966 3.057519587234856E-5 0.0 5.082606535448118 0.0 74-75 0.0011312822472768966 3.057519587234856E-5 0.0 5.518379514618765 0.0 76-77 0.0011312822472768966 3.057519587234856E-5 0.0 5.981792470858016 0.0 78-79 0.0011312822472768966 3.057519587234856E-5 0.0 6.489340722339003 0.0 80-81 0.0011618574431492452 3.057519587234856E-5 0.0 7.026302312249188 0.0 82-83 0.0011618574431492452 3.057519587234856E-5 0.0 7.573460729982801 0.0 84-85 0.0011771450410854195 3.057519587234856E-5 0.0 8.102503344162049 0.0 86-87 0.0011924326390215937 3.057519587234856E-5 0.0 8.64551882285496 0.0 88-89 0.0011924326390215937 3.057519587234856E-5 0.0 9.227150773934646 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5150 0.0 40.17769 1 GTATCAA 8005 0.0 34.80026 1 ATCAACG 9625 0.0 28.187416 3 TCAACGC 9655 0.0 28.00158 4 CAACGCA 9825 0.0 27.420525 5 TATCAAC 10015 0.0 27.098038 2 AACGCAG 10280 0.0 26.29915 6 GTACATG 8875 0.0 24.437317 1 TACATGG 9010 0.0 23.538322 2 ACGCAGA 11670 0.0 23.166689 7 CGCAGAG 11940 0.0 22.523645 8 ACATGGG 9315 0.0 21.436762 3 GCAGAGT 13375 0.0 19.575155 9 GAGTACT 7250 0.0 19.561094 12-13 TGCACCG 825 0.0 18.9723 5 GCACCGT 750 0.0 18.972298 6 GTACTTT 7455 0.0 18.800518 14-15 AGAGTAC 11605 0.0 18.75977 10-11 GTATAGA 1030 0.0 17.969378 1 CAGAGTA 12900 0.0 17.611881 10-11 >>END_MODULE