FastQCFastQC Report
Fri 27 May 2016
SRR939741_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939741_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1986286
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52200.26280203354401127No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41640.2096374842293607No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25430.12802788722268596No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32200.048.315711
GTATCAA58950.040.471991
TATCAAC71800.034.4839062
ATCAACG72500.034.4169923
TCAACGC72950.034.2697144
CAACGCA73700.033.727025
AACGCAG76650.032.6773576
ACGCAGA86300.028.638627
CGCAGAG86950.028.3706878
GCAGAGT92150.024.7620099
TGGTATC16200.024.0087322
GTGGTAT17650.023.6487581
GTACATG53950.022.858741
GAGTACT59600.021.64758512-13
TACATGG54750.021.3984932
AGAGTAC82700.020.9926810-11
CAGAGTA88950.020.90414410-11
ACATGGG55850.020.3851183
GTACTTT63350.020.14407214-15
AGTACTT61950.019.37162212-13
Produced by FastQC (version 0.11.5)