##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939741_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1986286 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.545915844948816 31.0 28.0 33.0 16.0 34.0 2 29.401367678169205 31.0 30.0 33.0 23.0 34.0 3 29.660913886519868 31.0 30.0 33.0 25.0 34.0 4 33.41105611175833 35.0 33.0 37.0 28.0 37.0 5 33.36451346885595 35.0 33.0 37.0 28.0 37.0 6 33.37197160932514 35.0 33.0 37.0 26.0 37.0 7 33.28010316741899 35.0 33.0 37.0 26.0 37.0 8 33.14049739060739 35.0 33.0 37.0 26.0 37.0 9 34.38604158716318 37.0 33.0 39.0 27.0 39.0 10-11 34.19491478065092 37.0 33.0 39.0 26.0 39.0 12-13 34.050967987490225 37.0 33.0 39.0 25.0 39.0 14-15 34.677311827199105 38.0 33.0 40.0 25.0 41.0 16-17 34.48104074639805 38.0 33.0 40.0 25.0 41.0 18-19 34.30611679284856 38.0 32.5 40.0 24.0 41.0 20-21 34.498977488639596 38.0 33.0 40.0 24.5 41.0 22-23 34.39207369935649 38.0 33.0 40.0 24.0 41.0 24-25 34.17050767110074 38.0 32.5 40.0 22.5 41.0 26-27 34.02928681972284 38.0 32.0 40.0 21.0 41.0 28-29 34.059201192577504 38.0 32.5 40.0 22.5 41.0 30-31 33.82508485686351 38.0 32.0 40.0 19.5 41.0 32-33 33.46639582618012 38.0 31.5 40.0 18.0 41.0 34-35 33.26530645637134 37.0 31.0 40.0 17.5 41.0 36-37 33.04013168294999 37.0 31.0 40.0 16.0 41.0 38-39 32.799289981402474 37.0 31.0 40.0 15.5 41.0 40-41 32.55518817531816 37.0 30.0 40.0 15.0 41.0 42-43 32.1540868233477 37.0 30.0 40.0 10.0 41.0 44-45 31.799353416376093 36.5 30.0 39.0 9.0 41.0 46-47 31.411891338910912 36.0 29.5 39.0 8.5 40.5 48-49 31.04350103660802 36.0 29.0 39.0 7.5 40.0 50-51 30.427739006366657 35.5 28.0 38.5 4.0 40.0 52-53 30.601945792297784 35.5 28.0 39.0 2.0 40.0 54-55 30.67101892678094 36.0 28.0 39.0 2.0 40.0 56-57 30.346389945858753 35.0 28.0 39.0 2.0 40.0 58-59 29.923295034048472 35.0 27.5 39.0 2.0 40.0 60-61 29.494435091421877 34.5 26.5 38.0 2.0 40.0 62-63 29.028793436594732 34.0 26.0 38.0 2.0 40.0 64-65 28.45391147095635 34.0 25.5 38.0 2.0 40.0 66-67 27.83996891686293 33.5 24.0 37.0 2.0 39.5 68-69 27.279165739475584 33.0 22.5 36.5 2.0 39.0 70-71 26.694932401476926 32.0 22.0 36.0 2.0 39.0 72-73 26.021473745472704 32.0 20.0 35.5 2.0 38.5 74-75 25.660126235597495 31.0 19.0 35.0 2.0 38.0 76-77 25.07064893978007 31.0 17.0 35.0 2.0 37.0 78-79 24.389027561992584 31.0 10.0 34.5 2.0 36.5 80-81 23.653288096477546 30.0 7.0 34.0 2.0 36.0 82-83 22.88903133788387 29.5 2.0 34.0 2.0 35.5 84-85 22.102764657254795 29.0 2.0 34.0 2.0 35.0 86-87 21.208489865004335 28.5 2.0 34.0 2.0 35.0 88-89 20.248918333009446 26.5 2.0 33.5 2.0 35.0 90-91 18.89229748384674 25.0 2.0 32.5 2.0 34.5 92-93 17.4222579225751 20.0 2.0 32.0 2.0 34.0 94-95 16.843493333789795 19.0 2.0 31.0 2.0 34.0 96-97 16.429623931296902 17.0 2.0 31.0 2.0 34.0 98-99 15.495203611161735 2.0 2.0 31.0 2.0 34.0 100-101 13.891176295860717 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.858085221160394 1101 2 1.1396762810901606 1101 3 1.2525417814662987 1101 4 1.2574300677188006 1101 5 1.2370781516326588 1101 6 1.3570189476454004 1101 7 1.174328837405497 1101 8 1.2006183879904881 1101 9 1.328138433999861 1101 10-11 1.3998729381368449 1101 12-13 1.3449190436512524 1101 14-15 1.556615858605774 1101 16-17 1.3995920484744673 1101 18-19 1.326197190412799 1101 20-21 1.3662031536400008 1101 22-23 1.665194484136677 1101 24-25 1.9402990620144678 1101 26-27 1.7820687228472565 1101 28-29 1.6930324994691404 1101 30-31 1.6847156850726392 1101 32-33 1.8322077787112363 1101 34-35 1.8074222532790927 1101 36-37 1.5493656901919408 1101 38-39 1.587016348406138 1101 40-41 1.7098527701539439 1101 42-43 1.5901300436788048 1101 44-45 1.6186519754783184 1101 46-47 1.439515746441348 1101 48-49 1.11864608834901 1101 50-51 1.2424224202062852 1101 52-53 1.60806585783126 1101 54-55 1.498748899041832 1101 56-57 1.518332160837499 1101 58-59 1.1975727162658671 1101 60-61 1.179539360080934 1101 62-63 0.9940686284942295 1101 64-65 0.9633614934775565 1101 66-67 1.04752445967571 1101 68-69 0.9773148615494094 1101 70-71 1.034817806933301 1101 72-73 0.8364561477656558 1101 74-75 0.8858183766624137 1101 76-77 1.0063647302518532 1101 78-79 0.5746939771452233 1101 80-81 0.5584287700826174 1101 82-83 0.588541166364724 1101 84-85 0.6056311399795682 1101 86-87 -0.1056549046907378 1101 88-89 -4.47533861465379 1101 90-91 -7.962260500567622 1101 92-93 -10.063509651515483 1101 94-95 -8.067174073403272 1101 96-97 -5.029997665109786 1101 98-99 -3.6303530281170318 1101 100-101 -2.6327227850804586 1102 1 1.7314965749385038 1102 2 1.1046071563260007 1102 3 0.9892981853544569 1102 4 0.9263451743456912 1102 5 0.9272289932684501 1102 6 1.0734117834322916 1102 7 0.8431047348040792 1102 8 1.121941972459382 1102 9 1.234908326418548 1102 10-11 1.3040400398323513 1102 12-13 1.1858756641574004 1102 14-15 1.6279943358044662 1102 16-17 1.1261803783117514 1102 18-19 1.0602042120419028 1102 20-21 1.3210434690543664 1102 22-23 1.4284691914018168 1102 24-25 1.5022271309188184 1102 26-27 1.5611405136790637 1102 28-29 1.279969189854107 1102 30-31 1.5572432711740731 1102 32-33 1.6814083275330702 1102 34-35 1.769469810104404 1102 36-37 1.6352287821343552 1102 38-39 1.523348691455638 1102 40-41 1.5115265502012534 1102 42-43 1.4519184815087058 1102 44-45 1.360837011962733 1102 46-47 1.6100117215018095 1102 48-49 1.3229973258874068 1102 50-51 1.5628214233838413 1102 52-53 1.4538884736359314 1102 54-55 1.3945075524245674 1102 56-57 1.651840138475432 1102 58-59 1.5116561553243884 1102 60-61 1.5311038292455628 1102 62-63 1.4839211194429929 1102 64-65 1.6103580016460768 1102 66-67 1.4341026862977877 1102 68-69 1.27717628509032 1102 70-71 1.3768807189583363 1102 72-73 1.2885112239985261 1102 74-75 1.07459241793795 1102 76-77 1.0392022889016488 1102 78-79 1.1745307226865727 1102 80-81 1.1823587309669357 1102 82-83 1.2266377754599382 1102 84-85 1.0853013195700179 1102 86-87 1.0160793470016571 1102 88-89 -0.13795397639213292 1102 90-91 -5.748233167046566 1102 92-93 -9.956508903793189 1102 94-95 -8.015552233162737 1102 96-97 -4.790613687037347 1102 98-99 -3.323111406245154 1102 100-101 -2.3957986973491145 1103 1 2.128135770115609 1103 2 1.2845993844311145 1103 3 1.0691274195529523 1103 4 1.0033705895213885 1103 5 1.0557351163342474 1103 6 0.8916465918065128 1103 7 0.9494879431934038 1103 8 0.938622401856577 1103 9 0.9448088569583177 1103 10-11 0.9416160721720743 1103 12-13 0.8477523608861901 1103 14-15 0.98334938726628 1103 16-17 1.0034991285478725 1103 18-19 0.9386873972234966 1103 20-21 0.8205185962921533 1103 22-23 0.896681331749285 1103 24-25 0.8796376326917823 1103 26-27 0.8772987688886573 1103 28-29 0.6177376305637239 1103 30-31 0.8777327386373557 1103 32-33 0.7246678278649981 1103 34-35 0.7466776489239422 1103 36-37 0.9459844909689537 1103 38-39 0.7989999924384605 1103 40-41 0.4206156193549333 1103 42-43 0.4444744634390503 1103 44-45 0.3497256309941612 1103 46-47 0.3522087930086393 1103 48-49 0.3716242911243697 1103 50-51 0.4060652719830138 1103 52-53 0.5419578054148886 1103 54-55 0.38054107951555594 1103 56-57 0.34442121714967655 1103 58-59 0.4423702521384527 1103 60-61 0.44758834212585796 1103 62-63 0.40901813320032687 1103 64-65 0.4388946186049978 1103 66-67 0.323086025298128 1103 68-69 0.28312799139904854 1103 70-71 -0.0951472058917524 1103 72-73 0.13676362707921896 1103 74-75 -0.08604968090543963 1103 76-77 -0.1017037845103772 1103 78-79 -0.32077915463622375 1103 80-81 -0.3434059101496274 1103 82-83 -0.6635167449223971 1103 84-85 -0.7615302839769882 1103 86-87 -0.4929446865038436 1103 88-89 -1.194655076870859 1103 90-91 -6.737588639486024 1103 92-93 -9.779821080301556 1103 94-95 -8.633139923344782 1103 96-97 -5.509252202173652 1103 98-99 -4.091977141363863 1103 100-101 -3.3190026610155066 1104 1 2.0291207560245432 1104 2 1.3158571964804189 1104 3 1.0743057532815605 1104 4 0.9645525298795334 1104 5 0.9792339743061191 1104 6 0.9968674444496273 1104 7 0.975644553816295 1104 8 1.0035455793655643 1104 9 1.1912320333989896 1104 10-11 1.1351482829205963 1104 12-13 1.030830473901041 1104 14-15 1.0767274402418607 1104 16-17 1.0964874604176131 1104 18-19 1.1411154622710242 1104 20-21 1.0286669509810977 1104 22-23 1.1183977070866078 1104 24-25 1.0811327387433494 1104 26-27 1.1442420221625085 1104 28-29 0.9195899809623072 1104 30-31 1.1745941376592057 1104 32-33 1.218391587391693 1104 34-35 1.1823228146942881 1104 36-37 1.3201533957543603 1104 38-39 1.0859712569167925 1104 40-41 0.9704105029834409 1104 42-43 1.0377758114382019 1104 44-45 0.8706388475393574 1104 46-47 0.7667006919802795 1104 48-49 0.9657076452898323 1104 50-51 1.0060032029143464 1104 52-53 0.9672739730718796 1104 54-55 1.0619631983249391 1104 56-57 1.1075586002493694 1104 58-59 1.1558564399011253 1104 60-61 1.0370126702094957 1104 62-63 1.2566633545230275 1104 64-65 1.1234054481084286 1104 66-67 1.183551767659143 1104 68-69 0.7974311192100529 1104 70-71 0.6216512907761746 1104 72-73 0.6916776598166443 1104 74-75 0.6931375241062341 1104 76-77 0.9215878204611343 1104 78-79 0.43941950911608174 1104 80-81 0.2620056366201098 1104 82-83 -0.10079194485744125 1104 84-85 0.06134831063400625 1104 86-87 0.46386442920956483 1104 88-89 -0.07809951490541778 1104 90-91 -3.0172046659118514 1104 92-93 -7.557218644971972 1104 94-95 -5.443025036810457 1104 96-97 -3.166737774587208 1104 98-99 -2.3349098140515494 1104 100-101 -1.569738195503902 1105 1 2.1125753954281024 1105 2 1.4247403440407673 1105 3 1.1928441750723628 1105 4 1.061824250555695 1105 5 1.0366917140270928 1105 6 1.1297219509824359 1105 7 0.8358630523768653 1105 8 0.9280233235155606 1105 9 1.151150834609595 1105 10-11 1.0386110422030939 1105 12-13 0.9829106392512941 1105 14-15 1.1153692059464433 1105 16-17 1.053390095687412 1105 18-19 1.1621119928681978 1105 20-21 1.1473744138880093 1105 22-23 1.0908513868681027 1105 24-25 0.9262925527243056 1105 26-27 0.8884399995744872 1105 28-29 1.013333245153639 1105 30-31 1.256777646923247 1105 32-33 1.4032266062150498 1105 34-35 1.3200049583423308 1105 36-37 1.2601711118538645 1105 38-39 1.300814395619085 1105 40-41 1.27820484859501 1105 42-43 0.9381854962392637 1105 44-45 0.9559197215336077 1105 46-47 1.1265589631842339 1105 48-49 0.7897683229306409 1105 50-51 0.7488377788352132 1105 52-53 0.9180497905960543 1105 54-55 1.0049801024365514 1105 56-57 0.8636640110414255 1105 58-59 0.7168315635447335 1105 60-61 0.9530848633149809 1105 62-63 0.7075395632777344 1105 64-65 0.7503132505739174 1105 66-67 0.7893478848806694 1105 68-69 0.7594913539483699 1105 70-71 0.6010164638772295 1105 72-73 0.7457973911224691 1105 74-75 0.7517187598041772 1105 76-77 0.7357282205497135 1105 78-79 0.5294807772935393 1105 80-81 0.4106909544026429 1105 82-83 0.2699373679055306 1105 84-85 0.2820193079027753 1105 86-87 0.5520256457150658 1105 88-89 0.2050605943438626 1105 90-91 -2.739030162331721 1105 92-93 -7.342329739679286 1105 94-95 -5.083333592210177 1105 96-97 -2.5362778941708797 1105 98-99 -1.2754014358128245 1105 100-101 -0.8913068084809446 1106 1 1.6693098275450744 1106 2 1.0231011469651534 1106 3 0.7423411715878956 1106 4 0.6584086153347641 1106 5 0.7745557128299723 1106 6 0.8236901845915341 1106 7 0.7567322458183625 1106 8 -0.11808497294224907 1106 9 -0.5167031169498699 1106 10-11 -0.5424310920879307 1106 12-13 -0.1253817569093698 1106 14-15 0.6543982574439084 1106 16-17 0.571769710471834 1106 18-19 0.8593761498617241 1106 20-21 0.7400012336148123 1106 22-23 0.6927494607901679 1106 24-25 0.8165219291539074 1106 26-27 0.7566960546419281 1106 28-29 0.4107085631382077 1106 30-31 0.7526693932196693 1106 32-33 0.6808870948958656 1106 34-35 0.9382490422261256 1106 36-37 1.0565169080444647 1106 38-39 0.6998832085479663 1106 40-41 0.6749955025209147 1106 42-43 0.9097208005424804 1106 44-45 0.9756187317732419 1106 46-47 0.964791930968925 1106 48-49 0.6702178836309542 1106 50-51 0.8958682839764123 1106 52-53 0.8966294951643441 1106 54-55 1.1155906328027427 1106 56-57 0.7912806766392393 1106 58-59 0.9301364653168953 1106 60-61 0.5138768853761633 1106 62-63 0.7884339817133572 1106 64-65 0.5725061148933754 1106 66-67 0.4059613225343206 1106 68-69 0.3771571869076027 1106 70-71 0.4482808346418885 1106 72-73 0.5787647482699079 1106 74-75 0.5442966259406923 1106 76-77 0.504346190151459 1106 78-79 0.4638761256336039 1106 80-81 0.18787558853334474 1106 82-83 0.06956561989894539 1106 84-85 -0.14497378728951205 1106 86-87 -0.03457817223743476 1106 88-89 -0.46060327192091677 1106 90-91 -3.979743089072354 1106 92-93 -8.407104531786857 1106 94-95 -5.938930761880329 1106 96-97 -3.644347327036055 1106 98-99 -2.567605630002525 1106 100-101 -1.7950984671311936 1107 1 1.778653763926787 1107 2 1.2199202324522318 1107 3 1.0087427620451521 1107 4 0.8187797220279407 1107 5 0.639366846030768 1107 6 0.7666987995683456 1107 7 0.6513644525778091 1107 8 0.569125099953709 1107 9 0.621799202466967 1107 10-11 0.6066742757053092 1107 12-13 0.748707043620783 1107 14-15 0.795219993693081 1107 16-17 0.8411534082849457 1107 18-19 0.9034450696761738 1107 20-21 0.7602132945823428 1107 22-23 0.8898998915390095 1107 24-25 0.7664887946277332 1107 26-27 0.8342308458944174 1107 28-29 0.6996416313953304 1107 30-31 0.7626097510725529 1107 32-33 0.8926167171622694 1107 34-35 1.0923245889458073 1107 36-37 0.8119841048635479 1107 38-39 0.802931584956184 1107 40-41 0.8320531565964657 1107 42-43 0.8480905818546063 1107 44-45 0.8984152857825158 1107 46-47 0.7162829846468561 1107 48-49 0.6241608922459321 1107 50-51 0.8571008883501712 1107 52-53 0.7491808536799205 1107 54-55 0.4741062060300436 1107 56-57 0.5851839238228038 1107 58-59 0.5789104878483755 1107 60-61 0.20208099405740754 1107 62-63 0.2629203965576252 1107 64-65 0.28522977294506546 1107 66-67 0.6113953848272295 1107 68-69 0.6607887309765275 1107 70-71 0.6616671832171015 1107 72-73 0.564516902014109 1107 74-75 0.5323681965172327 1107 76-77 0.6421858190447622 1107 78-79 0.5158973317303541 1107 80-81 0.3909902339939144 1107 82-83 0.5437206894814537 1107 84-85 0.6449533167529005 1107 86-87 0.4935861750123962 1107 88-89 0.5970508036258053 1107 90-91 -2.5089014721074765 1107 92-93 -3.9336121527278767 1107 94-95 -2.1132183695675373 1107 96-97 -0.5923261209393029 1107 98-99 -0.26674413232194283 1107 100-101 -0.04493279450031196 1108 1 1.6880797477066452 1108 2 0.977609921979468 1108 3 -0.40314148801007477 1108 4 -0.3197586621792894 1108 5 0.32125465748404025 1108 6 0.5933939574194227 1108 7 0.5332414445939619 1108 8 0.4692346467545363 1108 9 0.7213461385376192 1108 10-11 0.5828192200979103 1108 12-13 0.5988794783998586 1108 14-15 0.35349077715557087 1108 16-17 0.4453892179169543 1108 18-19 0.3282432195524194 1108 20-21 0.2677174758439804 1108 22-23 0.20914631774368075 1108 24-25 0.35213996695881633 1108 26-27 0.2917645552031871 1108 28-29 0.07112565243913593 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1.9713647835968828 2311 88-89 2.253631819008433 2311 90-91 2.9320460580508083 2311 92-93 3.778823557494565 2311 94-95 3.396951877387309 2311 96-97 2.6283995251839585 2311 98-99 2.5369194320147486 2311 100-101 2.4654474021956183 2312 1 0.9890377849675787 2312 2 0.30405063932060017 2312 3 -0.43808333408777855 2312 4 -0.8264134101957907 2312 5 -0.32078080555046284 2312 6 -0.5202715830358073 2312 7 -0.06243854974972862 2312 8 -0.18419019757072164 2312 9 -0.0962045788540209 2312 10-11 -0.11236029194447639 2312 12-13 -0.0011765796581002519 2312 14-15 -0.21133960934201212 2312 16-17 -0.5046327507814325 2312 18-19 -0.739129475628097 2312 20-21 -0.04691468734052506 2312 22-23 0.17421154205167255 2312 24-25 0.18969078535596395 2312 26-27 0.25130730326009854 2312 28-29 0.16431535593746815 2312 30-31 0.13127437801025366 2312 32-33 0.29257066828846945 2312 34-35 -0.022078524666504507 2312 36-37 0.17246797226049893 2312 38-39 0.12421693890524921 2312 40-41 0.22250185269012945 2312 42-43 0.32371965817618786 2312 44-45 0.2210896331651817 2312 46-47 0.08203708661264386 2312 48-49 0.2829251589102384 2312 50-51 0.2525047089307684 2312 52-53 0.27380666968219813 2312 54-55 0.24241454456601375 2312 56-57 0.3157568338016823 2312 58-59 0.2402376990429751 2312 60-61 0.11905871201100737 2312 62-63 0.40084799060728216 2312 64-65 0.23442561496791114 2312 66-67 0.331431367889504 2312 68-69 0.37472161474296684 2312 70-71 0.6069565457796635 2312 72-73 0.3670187432310996 2312 74-75 0.37933568630752035 2312 76-77 0.7497220419449278 2312 78-79 0.774238302977853 2312 80-81 0.7820552765522244 2312 82-83 0.6051633180456264 2312 84-85 0.9478853902362765 2312 86-87 1.3353836455713761 2312 88-89 1.6630899067944682 2312 90-91 2.5049090574398143 2312 92-93 3.3139458548318146 2312 94-95 2.9277893332873433 2312 96-97 2.287475249014694 2312 98-99 1.8305099083929335 2312 100-101 1.8312041477659111 2313 1 -7.384746743325767 2313 2 -2.840907026706031 2313 3 -1.6422243180783198 2313 4 -0.8338275391040426 2313 5 -0.6825393951200596 2313 6 -0.17404296284875898 2313 7 -0.2039160019757631 2313 8 -0.3116143256227488 2313 9 -0.016935776084174847 2313 10-11 -0.220244459343526 2313 12-13 -0.30766381943185905 2313 14-15 -0.26585925185293746 2313 16-17 -0.5943518397964525 2313 18-19 -0.47551946979198334 2313 20-21 -0.5313573580960806 2313 22-23 -1.2514243532998108 2313 24-25 -0.559399007151967 2313 26-27 -0.6194919491661253 2313 28-29 -0.785367594439812 2313 30-31 -0.1539426358871765 2313 32-33 -0.578452580704969 2313 34-35 -0.36714484280658866 2313 36-37 -0.16306353242364224 2313 38-39 -0.3627384103906337 2313 40-41 -0.4323165831327813 2313 42-43 -0.38145657181199155 2313 44-45 -0.287562381868959 2313 46-47 -1.3202373249288861 2313 48-49 -0.8377835992964933 2313 50-51 -0.735616485415779 2313 52-53 -1.2628781811229004 2313 54-55 -0.8581440690172712 2313 56-57 -0.6924296699130572 2313 58-59 -0.45310657167400237 2313 60-61 -0.09480289629615513 2313 62-63 -0.3465832825022055 2313 64-65 -0.19492758018171585 2313 66-67 -0.2491761981948919 2313 68-69 -0.49343396642282045 2313 70-71 -0.17142719498269088 2313 72-73 -0.22266924608477723 2313 74-75 -0.06290784205176436 2313 76-77 0.18047413305523818 2313 78-79 0.003303136993050515 2313 80-81 0.24155264242738994 2313 82-83 0.5536625115202014 2313 84-85 0.568554880152 2313 86-87 0.9982121238414479 2313 88-89 1.4179505634114733 2313 90-91 2.3029185782780885 2313 92-93 3.2821952271788675 2313 94-95 2.8591940259957447 2313 96-97 2.1268418583699926 2313 98-99 1.6499454488535434 2313 100-101 1.293805873799215 2314 1 0.5084276031608859 2314 2 0.03251716856604503 2314 3 -1.3791462161424626 2314 4 -0.30566774212332604 2314 5 -0.12309208830647123 2314 6 -3.5699497488574146 2314 7 -2.5239436280166387 2314 8 -2.5588446700785816 2314 9 -3.3577481587557685 2314 10-11 -2.424689403919075 2314 12-13 -1.2397720992524768 2314 14-15 -1.0741660834610158 2314 16-17 -1.9040707256349307 2314 18-19 -2.990150361284165 2314 20-21 -2.849877931113131 2314 22-23 -4.060787826126628 2314 24-25 -3.218112903692983 2314 26-27 -1.7422852349256317 2314 28-29 -1.6279905365896639 2314 30-31 -1.7929624011206684 2314 32-33 -2.162546100259444 2314 34-35 -1.3547897110697065 2314 36-37 -1.9180655616762223 2314 38-39 -3.099625149107517 2314 40-41 -2.527889580597389 2314 42-43 -1.3759688734701818 2314 44-45 -2.0426361009326506 2314 46-47 -1.6789660882914 2314 48-49 -2.4716749966758584 2314 50-51 -3.097372184559891 2314 52-53 -1.839402451453818 2314 54-55 -2.538409568119384 2314 56-57 -1.565345082987399 2314 58-59 -0.8230483266405777 2314 60-61 -1.3208461593342982 2314 62-63 -0.85569768220385 2314 64-65 -1.5354445851616134 2314 66-67 -0.8724709208846022 2314 68-69 -0.9338874495290952 2314 70-71 -0.8147905596871716 2314 72-73 -1.7107640364684684 2314 74-75 -1.938440814663906 2314 76-77 -1.087780129942164 2314 78-79 -1.9173960815488336 2314 80-81 -0.6596121798915675 2314 82-83 -0.4327974181846095 2314 84-85 -1.6160595070137198 2314 86-87 -0.40245645220303317 2314 88-89 0.6639578718547305 2314 90-91 1.7822029650990707 2314 92-93 2.6426651127572285 2314 94-95 2.456169381937613 2314 96-97 1.715810872701688 2314 98-99 1.5249348716683002 2314 100-101 1.4448651201395073 2315 1 -2.733798384633314 2315 2 -2.034811509049746 2315 3 -2.917834125982637 2315 4 -3.9760438592615763 2315 5 -3.8696303801628105 2315 6 -3.2070552829113304 2315 7 -5.445905212498026 2315 8 -3.0647436948177216 2315 9 -2.5131694961799838 2315 10-11 -3.9858038433279006 2315 12-13 -3.3429684766162637 2315 14-15 -2.1755246555962273 2315 16-17 -2.5771738883811715 2315 18-19 -2.1141708808662543 2315 20-21 -1.3325301192669556 2315 22-23 -0.8104510970940666 2315 24-25 -2.221493771802159 2315 26-27 -3.6708284933263116 2315 28-29 -3.2025607684062933 2315 30-31 -3.295933429054976 2315 32-33 -3.9980238878410788 2315 34-35 -3.7993100518428307 2315 36-37 -3.057612172327218 2315 38-39 -1.691351850466468 2315 40-41 -1.4521353660533727 2315 42-43 -2.94604423225789 2315 44-45 -6.337443204494793 2315 46-47 -2.908912746401974 2315 48-49 -1.9602636006958853 2315 50-51 -2.9682501783567226 2315 52-53 -3.205793510002888 2315 54-55 -1.6353242361363094 2315 56-57 -1.0843713177719252 2315 58-59 -2.360841356098934 2315 60-61 -2.6464359782526223 2315 62-63 -4.564992740391961 2315 64-65 -3.885690823488897 2315 66-67 -3.2024586883460486 2315 68-69 -1.7250208226348889 2315 70-71 -3.2922911278651945 2315 72-73 -2.160430148326011 2315 74-75 -1.4113349630987457 2315 76-77 -2.955860055878489 2315 78-79 -1.4955011326514338 2315 80-81 -1.1642252840820468 2315 82-83 -0.8012222198236358 2315 84-85 -2.340802740681948 2315 86-87 -1.7184346625759588 2315 88-89 -1.008739837385182 2315 90-91 -0.1318500826746707 2315 92-93 0.3104050509929337 2315 94-95 0.4129824212889588 2315 96-97 0.10396502942523966 2315 98-99 -0.31241247226656554 2315 100-101 -0.7432991468105783 2316 1 -7.2722725484280915 2316 2 -6.26645825236406 2316 3 -3.697077026602102 2316 4 -4.436863264834599 2316 5 -2.5384628045643893 2316 6 -3.0276875540655794 2316 7 -3.663706634892282 2316 8 -2.865971382221943 2316 9 -7.282390165144161 2316 10-11 -7.184941961983064 2316 12-13 -6.558083573996669 2316 14-15 -5.381059321509849 2316 16-17 -5.423257897417727 2316 18-19 -4.550472961576517 2316 20-21 -3.420688255405267 2316 22-23 -3.8434572809477032 2316 24-25 -3.5823527932495907 2316 26-27 -4.7430685574311795 2316 28-29 -4.786006818869996 2316 30-31 -7.083977708900434 2316 32-33 -5.195641899079213 2316 34-35 -5.764819284455015 2316 36-37 -4.7543274722387245 2316 38-39 -6.751577872295218 2316 40-41 -4.350209027297623 2316 42-43 -5.002264823878932 2316 44-45 -3.762176190803263 2316 46-47 -4.820324501479643 2316 48-49 -5.984822295143726 2316 50-51 -5.435363156894898 2316 52-53 -6.7462936592931335 2316 54-55 -5.699364578676473 2316 56-57 -4.870089682630081 2316 58-59 -3.746133839065461 2316 60-61 -3.9280056931981946 2316 62-63 -4.833351842779887 2316 64-65 -3.7823705095836786 2316 66-67 -4.569005353238019 2316 68-69 -3.5238477250962 2316 70-71 -2.977175176112919 2316 72-73 -2.5973413062427078 2316 74-75 -2.6020503241652904 2316 76-77 -3.368280644261908 2316 78-79 -5.359685684169158 2316 80-81 -6.166922625996275 2316 82-83 -3.570570819732982 2316 84-85 -2.366514612313921 2316 86-87 -2.4756624237259572 2316 88-89 -1.5183058794310682 2316 90-91 -1.213415166025392 2316 92-93 -1.4635960533951184 2316 94-95 -2.013409620282081 2316 96-97 -3.3543294824496286 2316 98-99 -4.58689921960992 2316 100-101 -3.977910785333936 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20182.0 3 7361.0 4 7206.0 5 7468.0 6 8311.0 7 9874.0 8 11531.0 9 13994.0 10 15962.0 11 18581.0 12 20936.0 13 23093.0 14 25054.0 15 26860.0 16 29197.0 17 31224.0 18 30152.0 19 29873.0 20 32024.0 21 34887.0 22 38201.0 23 42314.0 24 47042.0 25 52329.0 26 58971.0 27 65937.0 28 74033.0 29 83770.0 30 93796.0 31 105969.0 32 118020.0 33 130157.0 34 147393.0 35 168047.0 36 178748.0 37 137880.0 38 39304.0 39 605.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.548211466216586 19.66000818698592 13.812665226886384 22.97911511991111 2 15.735885557195948 19.916852449893973 40.36522218632289 23.982039806587185 3 19.608531058921976 24.035164657905746 29.45997852616934 26.896325757002938 4 13.019093265254373 16.56636816582551 37.12758604522431 33.28695252369581 5 13.94551805034043 36.798428077646435 34.510157963477404 14.745895908535733 6 32.24830830877259 36.02357024236735 17.548971070592756 14.1791503782673 7 28.473598583075376 32.2219229950688 21.025969218603592 18.27850920325223 8 26.046951851056257 35.2491354610723 19.893471861262302 18.810440826609142 9 26.22582677022402 16.956347863537086 20.21264524614371 36.60518012009519 10-11 24.914929452462356 26.485028188758786 27.577834256149586 21.02220810262927 12-13 25.885231118225022 23.74057843024301 27.822777553262107 22.551412898269863 14-15 22.80928176193834 25.330662201782538 25.99141933445762 25.868636701821497 16-17 22.401689907772564 28.364040097746006 26.752007446162423 22.482262548319003 18-19 22.26125467134955 27.033601946176066 28.927053950257836 21.778089432216547 20-21 22.80963205878385 26.383741645579672 29.108269252833345 21.698357042803128 22-23 22.537436395208868 26.218164811957852 28.998033460433607 22.246365332399673 24-25 22.39372614560996 26.36446417176902 29.12519626610593 22.11661341651509 26-27 22.1914976408131 26.61636729125776 28.957889939603405 22.234245128325735 28-29 22.10015725428765 26.91450534543458 28.973446432608768 22.011890967669004 30-31 22.31745157079225 26.616422104781385 28.866855695619986 22.199270628806378 32-33 22.108982479813267 26.706180168346595 28.801517697939456 22.38331965390068 34-35 22.549218714325466 26.826794678926998 28.66041994688413 21.96356665986341 36-37 22.616596011592108 26.56846948543493 28.598072456228085 22.21686204674488 38-39 22.214412914856492 26.766005765488853 28.630316723474753 22.389264596179903 40-41 22.593382539932428 26.634812558783523 28.658504587637395 22.113300313646658 42-43 22.3743762359553 26.609274025810542 28.554285162179283 22.46206457605488 44-45 22.35783452613863 26.853128049069653 28.568805173341026 22.22023225145069 46-47 22.55525264008243 27.01207103864014 28.06025780949224 22.372418511785195 48-49 22.399300380727155 26.986955331126623 28.113387790230316 22.50035649791591 50-51 22.37254169527117 27.22887978031741 28.0354510762024 22.36312744820902 52-53 22.511803902891366 27.185258492033267 27.88661489026983 22.41632271480554 54-55 22.26986537926662 27.139436873532023 28.024398209633596 22.56629953756776 56-57 22.276702086428525 27.273578297590596 27.97019834797494 22.479521268005943 58-59 22.298789610060602 27.00333551764867 27.948327358637915 22.749547513652814 60-61 22.063068762568395 27.199677724334236 28.107427576262996 22.629825936834372 62-63 22.10986489796155 27.15757044851314 28.13932588464052 22.593238768884788 64-65 21.963992446066484 27.081714299983577 28.06318587423513 22.89110737971481 66-67 21.998650429781357 27.187735307657867 27.832098329433876 22.981515933126904 68-69 22.11535550896113 26.99675498305851 28.03902496120308 22.848864546777286 70-71 22.066226201377905 26.945224382503486 27.814752307649616 23.173797108468992 72-73 22.094470464731295 27.081297694512863 27.737160334315824 23.087071506440022 74-75 22.033382467504445 27.37348708013564 27.564976597548235 23.028153854811677 76-77 22.19812676314778 27.158930838039897 27.565819346146498 23.077123052665822 78-79 22.266816552073653 27.262528463168117 27.497124027482133 22.973530957276097 80-81 22.247882539972625 27.28902010457428 27.39511397707888 23.067983378374215 82-83 22.269399570431187 27.326892072918156 27.356702906644635 23.04700545000602 84-85 22.303773554908226 27.300337682074133 27.334734264320698 23.061154498696943 86-87 22.247641246352657 27.226121862250707 27.4554230513563 23.070813840040337 88-89 22.350719402218918 27.189469377928 27.24208917503515 23.217722044817933 90-91 22.200015171820873 27.204035704351785 27.462639391104254 23.133309732723088 92-93 22.482625501203295 26.841096682061423 27.60614949501703 23.070128321718254 94-95 22.1989592280099 26.90918506542717 27.481003385035113 23.410852321527813 96-97 22.47080238529238 26.883315576605554 27.10331385984135 23.542568178260716 98-99 22.473799356572457 26.986176586237804 26.835231989243713 23.70479206794603 100-101 22.66798587200795 27.014770839432384 26.90123060438515 23.41601268417451 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2638.0 1 1567.5 2 493.5 3 494.5 4 448.5 5 431.0 6 543.5 7 904.5 8 1460.0 9 1776.5 10 1663.5 11 1448.0 12 1386.5 13 1433.5 14 1528.5 15 1630.5 16 1796.5 17 2033.0 18 2323.0 19 2759.0 20 3263.0 21 3922.0 22 4742.5 23 5781.0 24 7178.5 25 8941.5 26 11255.0 27 14120.0 28 17275.5 29 20312.0 30 23373.5 31 27141.5 32 31440.5 33 34917.5 34 37921.0 35 41394.5 36 45280.0 37 49045.0 38 52184.0 39 54926.5 40 58226.5 41 61675.0 42 64380.5 43 66900.5 44 70035.5 45 73209.0 46 74747.0 47 75845.5 48 76407.0 49 75433.5 50 73784.0 51 71648.5 52 69846.5 53 68723.0 54 67183.5 55 63024.5 56 57344.0 57 52257.5 58 47165.5 59 41288.0 60 34626.5 61 28590.5 62 23170.0 63 17766.5 64 13110.5 65 9742.5 66 7250.0 67 5504.0 68 4180.0 69 3052.5 70 2285.0 71 1657.0 72 1207.0 73 884.0 74 625.5 75 487.5 76 362.0 77 236.5 78 163.0 79 115.0 80 72.5 81 56.0 82 42.0 83 24.5 84 24.0 85 16.5 86 10.5 87 8.5 88 3.0 89 7.0 90 7.5 91 3.5 92 3.0 93 2.5 94 1.5 95 2.0 96 2.5 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13341482545816666 2 0.11795884379188093 3 0.12465475767336627 4 0.13628450283594606 5 0.12450372202190421 6 0.13935556108234162 7 0.17117373832368551 8 0.15848674360087117 9 0.1691095844203705 10-11 0.1624136705388851 12-13 0.15126220493926856 14-15 0.15639741708897914 16-17 0.15015461016188 18-19 0.1671712935599405 20-21 0.17260857701257523 22-23 0.20762367554319974 24-25 0.23166351673424673 26-27 0.2458356953631048 28-29 0.2407508284305483 30-31 0.2305307493482812 32-33 0.2316131715170927 34-35 0.22114136634905548 36-37 0.2312355823884375 38-39 0.21751651071396566 40-41 0.2234320737295636 42-43 0.21479786898764833 44-45 0.20762367554319974 46-47 0.2232810380781015 48-49 0.26222306354673997 50-51 0.2641110091900159 52-53 0.26519343135882745 54-55 0.2637585926699378 56-57 0.23752873453269066 58-59 0.23835943061573206 60-61 0.2080264372804319 62-63 0.1998453394929028 64-65 0.2017584577447558 66-67 0.21011576379232397 68-69 0.20993955553228488 70-71 0.21603132680792192 72-73 0.2480005397007279 74-75 0.2505429731670062 76-77 0.26564653831321366 78-79 0.26265099789254925 80-81 0.27654627782706015 82-83 0.2750107487038624 84-85 0.2509457349042384 86-87 0.25419300141067297 88-89 0.2563830183568731 90-91 0.44988486048836873 92-93 0.5997373993473246 94-95 0.3517116870380197 96-97 0.29280778296781024 98-99 0.3232414667374185 100-101 0.34292644664464234 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1986286.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.51071116758052 #Duplication Level Percentage of deduplicated Percentage of total 1 85.10629723027643 60.0090554254628 2 9.206705228524322 12.98342666347064 3 2.557729388847683 5.410419545456137 4 1.0406625713260993 2.93511431958745 5 0.5445368721996323 1.9197841057882994 6 0.34395486117415536 1.4551501122561674 7 0.2236940729517629 1.1040979717461012 8 0.16221451278518326 0.9150288526548684 9 0.12640443807845858 0.8021580141285458 >10 0.6422987404935491 8.000684878809047 >50 0.030681357913966527 1.4721581431033204 >100 0.013295876974620658 1.7489881835508203 >500 0.0012344011295928954 0.5774236588653232 >1k 2.1783549345756432E-4 0.3992507263550012 >5k 7.261183115252144E-5 0.2672593987654663 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5220 0.26280203354401127 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4164 0.2096374842293607 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2543 0.12802788722268596 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.034521715402515E-5 2 0.0 0.0 0.0 0.0 5.034521715402515E-5 3 0.0 0.0 0.0 0.0 5.034521715402515E-5 4 0.0 0.0 0.0 0.0 5.034521715402515E-5 5 0.0 0.0 0.0 0.0 5.034521715402515E-5 6 0.0 0.0 0.0 0.0 5.034521715402515E-5 7 0.0 0.0 0.0 0.0 5.034521715402515E-5 8 0.0 0.0 0.0 0.0 5.034521715402515E-5 9 0.0 0.0 0.0 0.0 5.034521715402515E-5 10-11 0.0 0.0 0.0 0.0 5.034521715402515E-5 12-13 0.0 0.0 0.0 0.0 5.034521715402515E-5 14-15 0.0 0.0 0.0 5.034521715402515E-5 7.551782573103772E-5 16-17 0.0 0.0 0.0 5.034521715402515E-5 1.006904343080503E-4 18-19 0.0 0.0 0.0 1.006904343080503E-4 1.006904343080503E-4 20-21 0.0 0.0 0.0 2.265534771931132E-4 1.2586304288506286E-4 22-23 2.5172608577012574E-5 0.0 0.0 2.768986943471383E-4 1.5103565146207544E-4 24-25 5.034521715402515E-5 0.0 0.0 6.544878230023269E-4 1.5103565146207544E-4 26-27 5.034521715402515E-5 0.0 0.0 0.0016110469489288048 1.5103565146207544E-4 28-29 5.034521715402515E-5 0.0 0.0 0.005386938235480691 1.5103565146207544E-4 30-31 5.034521715402515E-5 0.0 0.0 0.018929801649913456 1.5103565146207544E-4 32-33 5.034521715402515E-5 0.0 0.0 0.05210729975441603 1.5103565146207544E-4 34-35 5.034521715402515E-5 0.0 0.0 0.10104285082812847 2.013808686161006E-4 36-37 5.034521715402515E-5 0.0 0.0 0.1866045473813942 2.013808686161006E-4 38-39 5.034521715402515E-5 0.0 0.0 0.31176275722630076 2.013808686161006E-4 40-41 5.034521715402515E-5 0.0 0.0 0.440419959663412 2.013808686161006E-4 42-43 5.034521715402515E-5 0.0 0.0 0.5749927251161212 2.013808686161006E-4 44-45 7.551782573103772E-5 0.0 0.0 0.7419122925902917 2.265534771931132E-4 46-47 1.006904343080503E-4 0.0 0.0 0.9430917803377761 2.517260857701258E-4 48-49 1.006904343080503E-4 0.0 0.0 1.1600796662716246 2.517260857701258E-4 50-51 1.006904343080503E-4 0.0 0.0 1.3848458882557697 2.517260857701258E-4 52-53 1.006904343080503E-4 0.0 0.0 1.5948357890052087 4.027617372322012E-4 54-55 1.006904343080503E-4 0.0 0.0 1.815851292311379 4.5310695438622635E-4 56-57 1.006904343080503E-4 0.0 0.0 2.0844430258281035 4.782795629632389E-4 58-59 1.2586304288506286E-4 2.5172608577012574E-5 0.0 2.374280440983826 5.034521715402516E-4 60-61 1.7620826003908802E-4 5.034521715402515E-5 0.0 2.6697313478522227 5.286247801172641E-4 62-63 2.013808686161006E-4 5.034521715402515E-5 0.0 2.9575549543217843 5.537973886942766E-4 64-65 2.013808686161006E-4 5.034521715402515E-5 0.0 3.2561776098708846 5.537973886942766E-4 66-67 2.013808686161006E-4 5.034521715402515E-5 0.0 3.6003626869443774 5.537973886942766E-4 68-69 2.013808686161006E-4 5.034521715402515E-5 0.0 3.97918527341984 5.537973886942766E-4 70-71 2.013808686161006E-4 5.034521715402515E-5 0.0 4.376182483287906 5.537973886942766E-4 72-73 3.020713029241509E-4 5.034521715402515E-5 0.0 4.762556852336472 5.789699972712891E-4 74-75 3.020713029241509E-4 5.034521715402515E-5 0.0 5.146313270093028 6.293152144253144E-4 76-77 3.020713029241509E-4 5.034521715402515E-5 0.0 5.5616361390051585 6.544878230023269E-4 78-79 3.020713029241509E-4 5.034521715402515E-5 0.0 6.007896143858437 6.544878230023269E-4 80-81 3.020713029241509E-4 5.034521715402515E-5 0.0 6.472985259927322 6.544878230023269E-4 82-83 3.020713029241509E-4 5.034521715402515E-5 0.0 6.919320782606332 6.544878230023269E-4 84-85 4.027617372322012E-4 5.034521715402515E-5 0.0 7.342245779308719 6.544878230023269E-4 86-87 4.027617372322012E-4 5.034521715402515E-5 0.0 7.774434295967449 7.048330401563521E-4 88-89 4.027617372322012E-4 5.034521715402515E-5 0.0 8.241436530288187 8.055234744644024E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3220 0.0 48.31571 1 GTATCAA 5895 0.0 40.47199 1 TATCAAC 7180 0.0 34.483906 2 ATCAACG 7250 0.0 34.416992 3 TCAACGC 7295 0.0 34.269714 4 CAACGCA 7370 0.0 33.72702 5 AACGCAG 7665 0.0 32.677357 6 ACGCAGA 8630 0.0 28.63862 7 CGCAGAG 8695 0.0 28.370687 8 GCAGAGT 9215 0.0 24.762009 9 TGGTATC 1620 0.0 24.008732 2 GTGGTAT 1765 0.0 23.648758 1 GTACATG 5395 0.0 22.85874 1 GAGTACT 5960 0.0 21.647585 12-13 TACATGG 5475 0.0 21.398493 2 AGAGTAC 8270 0.0 20.99268 10-11 CAGAGTA 8895 0.0 20.904144 10-11 ACATGGG 5585 0.0 20.385118 3 GTACTTT 6335 0.0 20.144072 14-15 AGTACTT 6195 0.0 19.371622 12-13 >>END_MODULE