FastQCFastQC Report
Fri 27 May 2016
SRR939741_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939741_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1986286
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59730.30071198206099226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51840.2609896057264664No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34930.17585584351900985No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36300.051.3301961
GTATCAA61550.038.0899851
ATCAACG76300.030.3434093
TATCAAC78250.029.7695242
TCAACGC78000.029.6813284
CAACGCA78750.029.3387765
AACGCAG82950.027.9465376
ACGCAGA92050.025.0813887
CGCAGAG93500.024.4908568
GTACATG60350.023.4491161
TACATGG59650.022.9253942
ACATGGG59750.021.9388713
GCAGAGT103350.021.883169
TGGTATC17400.021.6805382
GAGTACT67050.021.1166512-13
GTACTTT69300.020.36305214-15
CAGAGTA99350.020.13210310-11
GTGGTAT19700.020.1138651
CATGGGG35300.019.635374
AGAGTAC94650.019.4890410-11
Produced by FastQC (version 0.11.5)