##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939741_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1986286 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.409931903059277 31.0 31.0 34.0 30.0 34.0 2 31.620195681790033 31.0 31.0 34.0 30.0 34.0 3 31.679078440869038 31.0 31.0 34.0 30.0 34.0 4 35.48952618102328 37.0 35.0 37.0 33.0 37.0 5 35.21176356274977 37.0 35.0 37.0 32.0 37.0 6 35.23595343268794 37.0 35.0 37.0 32.0 37.0 7 35.160814706442075 37.0 35.0 37.0 32.0 37.0 8 35.09737671211497 37.0 35.0 37.0 32.0 37.0 9 36.70924328117904 39.0 35.0 39.0 32.0 39.0 10-11 36.57000401754833 38.5 35.0 39.0 32.0 39.0 12-13 36.510651034141105 38.0 35.0 39.0 32.0 39.0 14-15 37.55638060178645 39.0 36.0 41.0 32.0 41.0 16-17 37.47123123256168 39.0 36.0 41.0 32.0 41.0 18-19 37.443614363691836 39.0 36.0 41.0 32.0 41.0 20-21 37.365850134371385 39.0 36.0 41.0 31.5 41.0 22-23 37.223502053581406 39.0 36.0 40.0 31.0 41.0 24-25 37.06178994364356 39.0 36.0 40.0 31.0 41.0 26-27 36.76206422438662 39.0 35.5 40.0 30.0 41.0 28-29 36.53064110606428 38.0 35.0 40.0 30.0 41.0 30-31 36.274837812882936 38.0 35.0 40.0 30.0 41.0 32-33 36.2327894875159 38.0 35.0 40.0 30.0 41.0 34-35 36.30809158399143 38.0 35.0 40.0 30.0 41.0 36-37 36.26852200539096 38.0 35.0 40.0 30.0 41.0 38-39 36.12941640831179 38.0 35.0 40.0 30.0 41.0 40-41 35.95322753118131 38.0 35.0 40.0 29.0 41.0 42-43 35.75404599337658 38.0 34.5 40.0 28.5 41.0 44-45 35.515508088966044 38.0 34.0 40.0 27.5 41.0 46-47 35.22156904896878 38.0 34.0 40.0 27.0 41.0 48-49 34.91251436097319 38.0 33.5 40.0 26.0 41.0 50-51 34.6397613435326 38.0 33.0 40.0 25.5 41.0 52-53 34.352803423072004 38.0 33.0 40.0 24.0 41.0 54-55 34.04443317830363 37.5 33.0 40.0 24.0 41.0 56-57 32.76304092159941 36.5 31.5 39.5 16.0 41.0 58-59 29.477259820589786 34.5 24.5 38.5 7.0 40.0 60-61 29.505322999809692 34.0 25.0 38.0 9.0 40.0 62-63 31.245186493787905 35.0 29.5 38.0 9.0 40.0 64-65 30.72051129595637 35.0 29.0 38.0 4.5 40.0 66-67 25.219212137627714 28.5 18.0 36.0 2.0 39.0 68-69 17.478753563182742 13.0 7.0 29.0 2.0 35.5 70-71 16.345221936820778 13.5 7.5 25.5 2.0 33.0 72-73 18.471837388976212 18.5 7.0 30.0 2.0 34.5 74-75 19.214835376174427 18.0 7.5 30.5 2.0 35.0 76-77 20.307310729673368 23.5 8.5 31.0 2.0 34.5 78-79 23.478103606429286 29.0 12.0 34.0 2.0 36.0 80-81 26.092865780657974 31.5 22.0 35.0 2.0 36.0 82-83 26.978823039582416 33.0 26.0 35.0 2.0 36.0 84-85 26.94461069553931 33.0 26.0 35.0 2.0 36.0 86-87 26.63188986883057 33.0 25.0 35.0 2.0 35.5 88-89 26.17101036809402 33.0 24.5 35.0 2.0 35.0 90-91 24.61305169547588 31.5 11.0 34.5 2.0 35.0 92-93 22.562493266327206 31.0 2.0 34.0 2.0 35.0 94-95 18.042124850097117 23.0 2.0 33.0 2.0 35.0 96-97 16.692130186690136 14.5 2.0 32.5 2.0 34.5 98-99 15.558852803674798 2.0 2.0 32.0 2.0 34.0 100-101 14.922357354378976 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.523188052109095 1101 2 0.5747728724892056 1101 3 0.578393056792958 1101 4 0.41500397366869635 1101 5 0.44084206923289315 1101 6 0.4263110520431397 1101 7 0.39007294438277285 1101 8 0.44659480608823543 1101 9 0.6435923878648353 1101 10-11 0.655012714334184 1101 12-13 0.7629429039371516 1101 14-15 0.8332977271690893 1101 16-17 0.8075786844107498 1101 18-19 0.7590341720861318 1101 20-21 0.8942808447514992 1101 22-23 0.66717729153509 1101 24-25 0.6424590445156184 1101 26-27 0.7178879724105016 1101 28-29 0.8165773610904736 1101 30-31 1.0827721577318172 1101 32-33 0.6923951326872952 1101 34-35 1.0080653877155115 1101 36-37 0.881314437589964 1101 38-39 0.8846114101923703 1101 40-41 1.2054745314113902 1101 42-43 0.9231095187941705 1101 44-45 0.943377530526611 1101 46-47 0.7792557547088208 1101 48-49 0.7580677741762045 1101 50-51 0.5462303234059434 1101 52-53 0.6323395883199865 1101 54-55 0.6180969037676647 1101 56-57 1.801566117956284 1101 58-59 -7.130153253541373 1101 60-61 -9.337419309261048 1101 62-63 -2.5054061412770032 1101 64-65 0.05283695882711825 1101 66-67 -0.6945058824727681 1101 68-69 -2.5804152258367665 1101 70-71 -3.4849159374276617 1101 72-73 -6.1751612073601 1101 74-75 -7.273979005969469 1101 76-77 -8.483086402233026 1101 78-79 -11.759891017933157 1101 80-81 -7.256057927455206 1101 82-83 -3.1811804746822716 1101 84-85 -2.420013457103007 1101 86-87 -2.1898541046272086 1101 88-89 -2.428975161445024 1101 90-91 -8.19032690943573 1101 92-93 -14.585860656345647 1101 94-95 -12.656707928313342 1101 96-97 -12.336594624120318 1101 98-99 -11.99371469025093 1101 100-101 -11.614759672874916 1102 1 0.3411002722537262 1102 2 0.47468671041380617 1102 3 0.41077249052778697 1102 4 0.3534988055260939 1102 5 0.43462194780448016 1102 6 0.34899825418021635 1102 7 0.40179604879075015 1102 8 0.46380085578381625 1102 9 0.4776929538768968 1102 10-11 0.4382417989385843 1102 12-13 0.6154169788549169 1102 14-15 0.6692770904556085 1102 16-17 0.7891308251217453 1102 18-19 0.7458963011647342 1102 20-21 0.7372254152224755 1102 22-23 0.7270541335526488 1102 24-25 0.7959767667995337 1102 26-27 0.7078037402812285 1102 28-29 0.5327318538046697 1102 30-31 0.6611780644215557 1102 32-33 0.6252983938743952 1102 34-35 0.8224019344618725 1102 36-37 0.847046293196243 1102 38-39 0.6683345820957314 1102 40-41 0.6319667857888405 1102 42-43 0.7200565127712579 1102 44-45 0.6670335023388532 1102 46-47 0.6284731773765131 1102 48-49 0.7882651032573307 1102 50-51 0.7876481272116251 1102 52-53 0.8123297382124761 1102 54-55 0.7774756509478777 1102 56-57 1.380051120798477 1102 58-59 -9.676815765023498 1102 60-61 -10.439617338606777 1102 62-63 -3.5638249775511284 1102 64-65 -0.6451085449784593 1102 66-67 -1.980668379717585 1102 68-69 -4.6001602620258915 1102 70-71 -4.842282900335784 1102 72-73 -7.328698440028287 1102 74-75 -7.909088944348293 1102 76-77 -9.238655562326802 1102 78-79 -12.528996693556673 1102 80-81 -8.654965135013 1102 82-83 -4.737654072913891 1102 84-85 -3.595016858237564 1102 86-87 -3.292401738966749 1102 88-89 -3.5835190244800152 1102 90-91 -9.023070712779491 1102 92-93 -14.75770706638221 1102 94-95 -12.438629289863965 1102 96-97 -12.349659726581473 1102 98-99 -11.93934979544354 1102 100-101 -11.655860670224667 1103 1 0.2414608018220079 1103 2 0.28230262768630254 1103 3 0.24435139415089324 1103 4 0.13336258147963775 1103 5 0.13783810902812377 1103 6 0.0978769964285604 1103 7 0.14862387433329616 1103 8 0.3476326158481555 1103 9 0.18686486927119006 1103 10-11 0.3260822915903674 1103 12-13 0.23940973588064907 1103 14-15 0.3603618095070118 1103 16-17 0.30134592310698594 1103 18-19 0.3785244969517123 1103 20-21 0.29386174991049785 1103 22-23 0.27557045723048645 1103 24-25 0.22698365895909234 1103 26-27 0.26031260509882514 1103 28-29 0.25051492078868876 1103 30-31 0.3668229427968015 1103 32-33 0.3043326279919327 1103 34-35 0.33232459331345865 1103 36-37 0.06670621882352634 1103 38-39 0.25755215737246573 1103 40-41 0.44238922065873965 1103 42-43 0.35169972882454203 1103 44-45 0.3251240988857518 1103 46-47 0.24022519995818215 1103 48-49 0.4704377796496573 1103 50-51 0.47422757594680576 1103 52-53 0.40534150939493685 1103 54-55 0.4505689730551481 1103 56-57 1.2143235580970924 1103 58-59 -6.402310008963852 1103 60-61 -8.601137823486663 1103 62-63 -2.449834487328065 1103 64-65 0.08989277732310441 1103 66-67 -1.5273294333490561 1103 68-69 -4.134645347004007 1103 70-71 -4.8943249117453576 1103 72-73 -7.7105588913379055 1103 74-75 -8.25920336391188 1103 76-77 -9.15150779377815 1103 78-79 -12.289809882030521 1103 80-81 -8.63913529633379 1103 82-83 -4.788932387708016 1103 84-85 -3.9170674511144092 1103 86-87 -3.6150797504410903 1103 88-89 -3.568003569743208 1103 90-91 -9.066358548144851 1103 92-93 -14.642612859142474 1103 94-95 -12.52973721665703 1103 96-97 -12.528012570609258 1103 98-99 -11.94202543217222 1103 100-101 -11.580384236876762 1104 1 0.18373614063793298 1104 2 0.21956158924194114 1104 3 0.22055618575479485 1104 4 0.26108619571557057 1104 5 0.07996110620563712 1104 6 0.08987809892905574 1104 7 0.10619149329545507 1104 8 0.2594509511151344 1104 9 0.1986131231112438 1104 10-11 0.26518569801778114 1104 12-13 0.20658716367862695 1104 14-15 0.1682954329945403 1104 16-17 0.18799081752547409 1104 18-19 0.31288180967085566 1104 20-21 0.23200175038055448 1104 22-23 0.2719103051452407 1104 24-25 0.3071468760384519 1104 26-27 0.16202789075543222 1104 28-29 0.16158351298529539 1104 30-31 0.2248777131412396 1104 32-33 0.15653645383958548 1104 34-35 0.2671294229493597 1104 36-37 0.23969869575327607 1104 38-39 0.38213428618127665 1104 40-41 0.44667615282290996 1104 42-43 0.37224138560929987 1104 44-45 0.23259140312247695 1104 46-47 0.2190384949176618 1104 48-49 0.16802595298153733 1104 50-51 -0.11157089294494682 1104 52-53 0.07456379785264744 1104 54-55 0.14071366556220255 1104 56-57 0.5744361157059501 1104 58-59 -6.532076560893547 1104 60-61 -8.709624727020007 1104 62-63 -2.6090052189311557 1104 64-65 -0.4886066468554162 1104 66-67 -1.7567858535983127 1104 68-69 -4.2256841118615895 1104 70-71 -4.614911758230708 1104 72-73 -7.650392597512976 1104 74-75 -8.78001487692288 1104 76-77 -9.212805753083916 1104 78-79 -12.148490577802345 1104 80-81 -8.481539046544782 1104 82-83 -4.800278527994795 1104 84-85 -3.916758067254076 1104 86-87 -3.614152774005966 1104 88-89 -3.753491265965863 1104 90-91 -9.298301577072671 1104 92-93 -15.224202596486688 1104 94-95 -12.530126189942761 1104 96-97 -12.267963150383501 1104 98-99 -11.684879049591729 1104 100-101 -11.341027533096852 1105 1 0.16470166806097808 1105 2 0.2790286299151532 1105 3 0.20845608979259111 1105 4 0.1452598134907248 1105 5 0.0575989596049169 1105 6 0.2624491478697806 1105 7 0.08900097140868723 1105 8 0.20548328299673102 1105 9 0.1250854047972254 1105 10-11 0.38189567070545394 1105 12-13 0.3610774567324384 1105 14-15 0.37205272243132015 1105 16-17 0.4547982287604313 1105 18-19 0.28614083380904276 1105 20-21 0.2510657497900226 1105 22-23 0.298150801196023 1105 24-25 0.3798685118249736 1105 26-27 0.4937146110625079 1105 28-29 0.3830738798561839 1105 30-31 0.37405465268505367 1105 32-33 0.5463777471148461 1105 34-35 0.5144889859522337 1105 36-37 0.3294934842673314 1105 38-39 0.44708741233466043 1105 40-41 0.41774207147648923 1105 42-43 0.4949098730973063 1105 44-45 0.23743306548314536 1105 46-47 0.26533287743777834 1105 48-49 0.2632846818514025 1105 50-51 0.2759835471524852 1105 52-53 0.3507666471919819 1105 54-55 0.3194292483480581 1105 56-57 0.9320835653257902 1105 58-59 -6.717994476299172 1105 60-61 -8.401220652317118 1105 62-63 -2.451912135591673 1105 64-65 0.033502307156542344 1105 66-67 -1.3773749139068698 1105 68-69 -3.554390836597694 1105 70-71 -4.264092388628583 1105 72-73 -5.766624973684824 1105 74-75 -6.265863404272109 1105 76-77 -8.12031295024934 1105 78-79 -11.518464741823902 1105 80-81 -7.563574921646282 1105 82-83 -3.4622413241858325 1105 84-85 -2.5281731407020693 1105 86-87 -2.39323522768575 1105 88-89 -2.4649196264884843 1105 90-91 -7.5812505694695105 1105 92-93 -10.872786046697065 1105 94-95 -11.458992359574413 1105 96-97 -12.00964987069058 1105 98-99 -11.468431865713379 1105 100-101 -11.104107919898357 1106 1 0.13198228543202006 1106 2 0.12070545071080829 1106 3 0.16512553548403375 1106 4 0.11683894087694568 1106 5 0.1413668896589968 1106 6 0.1912133369289748 1106 7 0.00920362644620809 1106 8 0.18619043791667877 1106 9 0.031593880822796905 1106 10-11 0.232117137602998 1106 12-13 0.17957592826881097 1106 14-15 0.23998277651541855 1106 16-17 0.1303026217572878 1106 18-19 0.17938181801135045 1106 20-21 0.22554104853518453 1106 22-23 0.2765009155635809 1106 24-25 0.27612067625773307 1106 26-27 0.35963265120872734 1106 28-29 0.41061593932731455 1106 30-31 0.2080955684806014 1106 32-33 0.16080815392132308 1106 34-35 0.19873490826780227 1106 36-37 0.19796117276003855 1106 38-39 0.1735343222728858 1106 40-41 0.32377875460353067 1106 42-43 0.0735428066786099 1106 44-45 0.12331070288454526 1106 46-47 0.005416034409925885 1106 48-49 0.03227311683291134 1106 50-51 0.13216046414515148 1106 52-53 0.08528983067174778 1106 54-55 -0.25869597878376993 1106 56-57 0.553128264870459 1106 58-59 -6.962048218362916 1106 60-61 -9.206925135097855 1106 62-63 -2.7389550394587907 1106 64-65 -0.5529332729114387 1106 66-67 -1.734577077270771 1106 68-69 -4.448218622570032 1106 70-71 -4.847231190227593 1106 72-73 -6.531929918211212 1106 74-75 -7.086333487001539 1106 76-77 -8.885876557851041 1106 78-79 -12.09517052860857 1106 80-81 -8.33074010260355 1106 82-83 -4.450946298640183 1106 84-85 -3.5830315173240557 1106 86-87 -3.2997870992520504 1106 88-89 -3.2092805576755588 1106 90-91 -8.534702545783668 1106 92-93 -11.25355690541572 1106 94-95 -11.49378254320382 1106 96-97 -11.957946969816257 1106 98-99 -11.425033545954513 1106 100-101 -11.170732901193382 1107 1 0.11475233182035538 1107 2 0.13130849908721842 1107 3 0.049154693867070165 1107 4 -0.018349925922265697 1107 5 0.10028007720041643 1107 6 0.026866087094646218 1107 7 0.13113868277490326 1107 8 0.08811224043490284 1107 9 0.14557665086918092 1107 10-11 0.11283284336840183 1107 12-13 0.14213391368961936 1107 14-15 0.1611226042158762 1107 16-17 0.18132979206875888 1107 18-19 0.06573039322672969 1107 20-21 0.22023952434698657 1107 22-23 0.18935711171997838 1107 24-25 0.3768496358336151 1107 26-27 0.35201171018817945 1107 28-29 0.27741514409868984 1107 30-31 0.3379829110916006 1107 32-33 0.36458548990542283 1107 34-35 0.3017832846760271 1107 36-37 0.13467422776334814 1107 38-39 0.053918682776533444 1107 40-41 0.03583972213169062 1107 42-43 0.13152822748708815 1107 44-45 0.3830853881065508 1107 46-47 0.20654260829991955 1107 48-49 0.05844939251215919 1107 50-51 0.14939041775681972 1107 52-53 -1.972468630384583E-4 1107 54-55 0.0385207210174201 1107 56-57 0.6104509951554178 1107 58-59 -6.751975322405329 1107 60-61 -9.13667993960415 1107 62-63 -2.448033899631092 1107 64-65 -0.14272783884914375 1107 66-67 -1.872748051425841 1107 68-69 -3.903155666970296 1107 70-71 -5.027814357888293 1107 72-73 -6.571360004360981 1107 74-75 -7.185405720268603 1107 76-77 -9.463411349103527 1107 78-79 -12.860246738018773 1107 80-81 -8.971230493590959 1107 82-83 -4.724968830117984 1107 84-85 -3.6877366200410897 1107 86-87 -3.436632692360071 1107 88-89 -3.31166624356025 1107 90-91 -8.816346018282012 1107 92-93 -12.147195076389877 1107 94-95 -12.220754047511308 1107 96-97 -12.36450760599916 1107 98-99 -11.633118370341524 1107 100-101 -11.102807122532019 1108 1 -0.07403873132458472 1108 2 0.07244535144247166 1108 3 -0.004654875023444305 1108 4 -0.06296928460012907 1108 5 -0.22865515494412847 1108 6 -0.10197627821462873 1108 7 -0.1024232111675687 1108 8 -0.042976961316043116 1108 9 -0.10569215353793737 1108 10-11 0.03619967187581352 1108 12-13 -0.045230786995162475 1108 14-15 0.06170234572149269 1108 16-17 -0.06534075156287855 1108 18-19 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62-63 1.4578266597266314 2311 64-65 1.4262685075561983 2311 66-67 -0.4975419136213972 2311 68-69 -3.5931761634603827 2311 70-71 -0.7137569618515354 2311 72-73 -1.1875070747410597 2311 74-75 1.105142876059034 2311 76-77 4.162615967820887 2311 78-79 5.254040253894054 2311 80-81 3.082659734362853 2311 82-83 1.6759587900020065 2311 84-85 1.3583983694320985 2311 86-87 1.4086545810496176 2311 88-89 1.4883218807909877 2311 90-91 3.0552367271334013 2311 92-93 4.922536533948094 2311 94-95 8.804695907625131 2311 96-97 9.438364688258893 2311 98-99 9.638814780018851 2311 100-101 8.75141591681303 2312 1 -0.26756932841865577 2312 2 -0.269602737280767 2312 3 -0.30094544767418085 2312 4 -0.14676547568235065 2312 5 -0.15767656306194056 2312 6 -0.16674404129610565 2312 7 -0.1828892505900157 2312 8 -0.24770300912195609 2312 9 -0.24547569873032415 2312 10-11 -0.28614729215107104 2312 12-13 -0.33072942594591836 2312 14-15 -0.3359866482131224 2312 16-17 -0.39142491945423785 2312 18-19 -0.389251076529348 2312 20-21 -0.23930137428498455 2312 22-23 -0.4241108555321844 2312 24-25 -0.39299735497579746 2312 26-27 -0.48012833742517813 2312 28-29 -0.5704148765199903 2312 30-31 -0.5182651978789181 2312 32-33 -0.28160271125140923 2312 34-35 -0.4029734754314873 2312 36-37 -0.44111144424763893 2312 38-39 -0.4263259075877457 2312 40-41 -0.40550744357488355 2312 42-43 -0.3044456840547909 2312 44-45 -0.26848905535887013 2312 46-47 -0.12431795954763913 2312 48-49 -0.20593652656874895 2312 50-51 -0.2418481936334942 2312 52-53 -0.026612474871377856 2312 54-55 -0.14830693959648045 2312 56-57 0.9413047737459408 2312 58-59 4.541004733414169 2312 60-61 4.267458850520722 2312 62-63 1.742011278446693 2312 64-65 1.4200767737130455 2312 66-67 -0.22987581195989293 2312 68-69 -1.08823450352779 2312 70-71 0.2200048884746586 2312 72-73 7.3557976141447625 2312 74-75 5.653867179821578 2312 76-77 5.747400247537858 2312 78-79 5.866108862601706 2312 80-81 3.3425016643153533 2312 82-83 1.8353281851179943 2312 84-85 1.541586181088288 2312 86-87 1.4396270469029417 2312 88-89 1.7711849787274048 2312 90-91 3.385928825601976 2312 92-93 5.457224729939188 2312 94-95 9.072572408009467 2312 96-97 9.654593968665694 2312 98-99 10.059726047929843 2312 100-101 9.34451267066131 2313 1 -0.1800884335740207 2313 2 -0.2531852454715029 2313 3 -0.36782318550568505 2313 4 -0.3269010349405619 2313 5 -0.275914076188279 2313 6 -0.23511161218324617 2313 7 -0.3614983657543718 2313 8 -0.36742878063561335 2313 9 -0.26407942065571177 2313 10-11 -0.36336725461491426 2313 12-13 -0.3146273598826781 2313 14-15 -0.4839790937419082 2313 16-17 -0.28393796213660494 2313 18-19 -0.25617467657540516 2313 20-21 -0.3211569634225313 2313 22-23 -0.40698397564886335 2313 24-25 -0.40441783373401563 2313 26-27 -0.26825622411204364 2313 28-29 -0.44819876707267525 2313 30-31 -0.3821539200060613 2313 32-33 -0.4396231366677341 2313 34-35 -0.5657231218692402 2313 36-37 -0.49690952059346927 2313 38-39 -0.7351397197908298 2313 40-41 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2314 4 -0.23588866511828854 2314 5 -0.38815544442162064 2314 6 -0.2629535059851733 2314 7 -0.3731009503483449 2314 8 -0.48251717456446386 2314 9 -0.3543237693048127 2314 10-11 -0.46323645009069736 2314 12-13 -0.4255794867587568 2314 14-15 -0.5429896019795706 2314 16-17 -0.5008572276568586 2314 18-19 -0.5843438950859507 2314 20-21 -0.5059146322735799 2314 22-23 -0.6155179816911769 2314 24-25 -0.5228065053335698 2314 26-27 -0.5416031079949164 2314 28-29 -0.6587392063941024 2314 30-31 -0.5831362075300035 2314 32-33 -0.6260447534571014 2314 34-35 -0.6922348758642798 2314 36-37 -0.5216246809122538 2314 38-39 -0.5651360491158002 2314 40-41 -0.56339554005406 2314 42-43 -0.39757640006673256 2314 44-45 -0.4592541914374664 2314 46-47 -0.4118591266802767 2314 48-49 -0.5886322135958721 2314 50-51 -0.4506965603743893 2314 52-53 -0.4708225200466316 2314 54-55 -0.3795466724938521 2314 56-57 1.0016600352411444 2314 58-59 4.096220520269114 2314 60-61 4.0252950191539405 2314 62-63 1.508727306183669 2314 64-65 1.4564664457975844 2314 66-67 1.9495662894790833 2314 68-69 -0.01531339436479584 2314 70-71 2.9896024180518097 2314 72-73 8.626658885843892 2314 74-75 10.158793710804535 2314 76-77 9.108164514861617 2314 78-79 6.7001211080960275 2314 80-81 3.614659750053555 2314 82-83 2.1044350656563076 2314 84-85 1.6346402887191154 2314 86-87 1.6696070852861098 2314 88-89 1.6309374428320353 2314 90-91 2.9417058478263023 2314 92-93 5.074619867281431 2314 94-95 8.843186775634923 2314 96-97 9.624719266806558 2314 98-99 10.058119838702083 2314 100-101 9.158759558939437 2315 1 -0.6329274706091752 2315 2 -0.529362723342576 2315 3 -0.5355419643863648 2315 4 -0.3228165599853057 2315 5 -0.6120881888551466 2315 6 -0.6074958218787785 2315 7 -0.5248709084368599 2315 8 -0.6935468468260169 2315 9 -0.9409588018246424 2315 10-11 -0.8321966341803844 2315 12-13 -0.6967754513579294 2315 14-15 -0.9052465387647572 2315 16-17 -0.945419764003546 2315 18-19 -0.745929337362405 2315 20-21 -0.8683614491397762 2315 22-23 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1.2901854174445049 2315 90-91 2.710945776035338 2315 92-93 4.637168956191264 2315 94-95 8.416298753496033 2315 96-97 9.25179520547007 2315 98-99 9.632736234982861 2315 100-101 8.852320890414493 2316 1 -0.4965350962827806 2316 2 -0.42481278845470527 2316 3 -0.49903960092398236 2316 4 -0.3657442495479373 2316 5 -0.6223671817395839 2316 6 -0.5268570574983542 2316 7 -0.4550300554319904 2316 8 -0.5564134173233839 2316 9 -0.6680283602852626 2316 10-11 -0.7205046001637854 2316 12-13 -0.735015562228412 2316 14-15 -0.815414957942977 2316 16-17 -0.9340379569081207 2316 18-19 -0.9659217739230428 2316 20-21 -0.7412988155879603 2316 22-23 -0.7739826019066598 2316 24-25 -2.223472414055344 2316 26-27 -1.368991311124148 2316 28-29 -1.0424961767801122 2316 30-31 -0.7616508385732601 2316 32-33 -0.6218557544857362 2316 34-35 -0.6943733632228444 2316 36-37 -0.5066891062276184 2316 38-39 -0.8311577858923727 2316 40-41 -0.6857105879670016 2316 42-43 -1.0151356821846491 2316 44-45 -0.8187772275481251 2316 46-47 -0.7219177997353299 2316 48-49 -0.9658316072399629 2316 50-51 -0.9942716239876219 2316 52-53 -0.9865126559353214 2316 54-55 -0.8222812293667872 2316 56-57 0.30725901472249006 2316 58-59 3.6487877202041545 2316 60-61 3.719051448150747 2316 62-63 1.152772363300226 2316 64-65 0.7729710340710945 2316 66-67 5.916208255263285 2316 68-69 8.495800310902922 2316 70-71 5.062211169608304 2316 72-73 9.059675506492471 2316 74-75 10.028323987995805 2316 76-77 8.567653375778733 2316 78-79 5.991490010700108 2316 80-81 2.9041642435839563 2316 82-83 1.6312744354792734 2316 84-85 1.1896352808689734 2316 86-87 1.0558696725851178 2316 88-89 1.232772752235963 2316 90-91 2.6439045647338872 2316 92-93 4.652307526314505 2316 94-95 8.462020014225143 2316 96-97 9.391157196534241 2316 98-99 9.691553667406541 2316 100-101 8.46069908576527 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 28.0 5 86.0 6 192.0 7 482.0 8 1247.0 9 2149.0 10 3515.0 11 5240.0 12 7377.0 13 9729.0 14 13052.0 15 17731.0 16 23357.0 17 30239.0 18 33062.0 19 34429.0 20 35612.0 21 35462.0 22 34098.0 23 33076.0 24 33795.0 25 37027.0 26 48643.0 27 69234.0 28 96798.0 29 125374.0 30 150017.0 31 161403.0 32 167985.0 33 175269.0 34 177418.0 35 170169.0 36 140415.0 37 84948.0 38 26447.0 39 1170.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.49861393318176 19.82224102203859 15.054020097339407 23.625124947440245 2 15.938846819994362 20.858792846497764 39.30156684255045 23.900793490957426 3 19.351000932938675 24.2591243466786 30.14746271392012 26.2424120064626 4 13.476558763440917 17.2912158672014 36.79530540919082 32.43691996016687 5 13.629475508445971 37.19970578900437 34.6782067235273 14.492611979022351 6 32.092254729059256 36.72699207514739 17.15390002330987 14.026853172483477 7 28.163567582916055 32.37529741437034 21.017466769639416 18.443668233074188 8 25.873302042055435 35.85254467767181 19.60425578832422 18.66989749194853 9 25.586144190715736 16.903104588161018 20.176298881429965 37.33445233969327 10-11 24.77019925630045 26.746047648727323 27.53578789761394 20.947965197358286 12-13 25.57718274206232 23.83846032243091 27.936133064422748 22.64822387108402 14-15 22.340930859532822 25.52106762255621 26.05311681495457 26.0848847029564 16-17 22.05470410605522 28.551477481087815 26.906019576234236 22.487798836622723 18-19 21.94966984507854 27.183036297532375 28.98773048604554 21.879563371343544 20-21 22.469120761058576 26.471389316543537 29.257997086018833 21.80149283637905 22-23 22.245109717331744 26.340038644988688 29.108975243242917 22.305876394436652 24-25 22.181146118937555 26.508846158106138 29.15705492562501 22.152952797331302 26-27 22.00281178533265 26.767264978672472 28.972867656035834 22.257055579959044 28-29 21.97621587113947 26.972438634697056 28.985664122748478 22.065681371414993 30-31 22.178535473497305 26.685588080616906 28.92612416384897 22.209752282036817 32-33 21.90994675726817 26.71392585286922 28.88376037523401 22.4923670146286 34-35 22.40193972054592 26.846587226128634 28.733444748448782 22.018028304876662 36-37 22.463413240097722 26.579954058667226 28.732393373228383 22.224239328006664 38-39 22.054261770212577 26.822699764026716 28.72879085595961 22.3942476098011 40-41 22.433297033255485 26.64004381188586 28.726824078911754 22.199835075946897 42-43 22.156753239820766 26.622856360140563 28.68030746949024 22.540082930548436 44-45 22.193137133201173 26.840411744814098 28.677178760913097 22.289272361071628 46-47 22.42828891011834 26.99345473737115 28.180258859421137 22.397997493089377 48-49 22.25035081707077 26.974754516179633 28.242203148830285 22.532691517919307 50-51 22.229342198921294 27.18992368587961 28.158195754820902 22.422538360378194 52-53 22.434473119898524 27.05878768820024 28.00458838597531 22.50215080592593 54-55 22.217637342261177 27.062081932168564 28.103574200659466 22.616706524910793 56-57 22.252237420865555 27.10068223720153 28.150774396326845 22.496305945606075 58-59 20.837161063529287 26.700317711120093 29.800151257182623 22.662369968168 60-61 22.128207588620604 26.838621866903694 28.439449110384984 22.593721434090714 62-63 21.9783212840083 27.04273818394184 28.270183458525484 22.708757073524378 64-65 21.343459490120694 26.97804808874043 28.702209056817825 22.976283364321056 66-67 21.717893076363517 26.52218244529992 29.135480265470086 22.62444421286647 68-69 22.576460006431322 25.63058824869652 29.72361875399045 22.06933299088171 70-71 21.613653140426734 25.986596403650676 29.796031445849426 22.603719010073167 72-73 21.442059407444624 26.216789265716923 29.667618737011246 22.673532589827207 74-75 21.927298418948066 26.409537676348886 28.967619745345452 22.695544159357592 76-77 20.300210452874726 26.81658516339062 29.620255420784797 23.262948962949853 78-79 21.259782998635558 27.17492457891739 28.38358329589562 23.181709126551432 80-81 21.791940471710344 27.194646280174144 27.648559557867525 23.36485369024799 82-83 21.909385039950237 27.25562589630332 27.454540099638347 23.380448964108094 84-85 21.904943627159152 27.199074452828885 27.377800108695403 23.51818181131656 86-87 21.973281107220444 27.175686663461274 27.366776841612396 23.484255387705886 88-89 21.909343321829443 27.16523560314859 27.282590363417132 23.64283071160483 90-91 21.579148151549617 27.209324857169097 27.52443087145774 23.687096119823547 92-93 21.417245276681246 27.04046714468503 27.701411510871914 23.8408760677618 94-95 20.309768916887833 26.773478960488227 28.970947015265754 23.945805107358193 96-97 20.073829063403736 26.726988029235798 29.16375515783353 24.03542774952693 98-99 20.407156340079258 26.81526793039796 28.436878966539282 24.340696762983494 100-101 20.193797607028767 26.98060658944219 28.374610663165562 24.45098514036348 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 320.0 1 234.5 2 160.5 3 187.5 4 233.5 5 287.5 6 357.5 7 530.5 8 776.0 9 978.0 10 1069.5 11 1165.0 12 1361.5 13 1554.0 14 1754.0 15 1958.0 16 2060.0 17 2231.0 18 2583.5 19 2898.5 20 3347.5 21 4006.5 22 4817.5 23 5958.5 24 7315.5 25 8990.0 26 11348.0 27 14139.0 28 17234.5 29 20405.5 30 23691.5 31 27658.5 32 32086.0 33 36174.0 34 39742.0 35 43405.5 36 47521.0 37 51582.5 38 54950.0 39 58355.0 40 61787.0 41 64984.5 42 68379.0 43 71386.5 44 74002.5 45 76416.5 46 77992.5 47 78833.5 48 78550.0 49 77371.5 50 76032.0 51 73988.5 52 71626.0 53 69248.5 54 66185.5 55 61433.0 56 55517.5 57 49171.5 58 42732.0 59 36020.0 60 29240.0 61 23458.0 62 18177.5 63 13427.0 64 9761.0 65 7155.5 66 5258.5 67 3802.5 68 2687.0 69 1884.0 70 1369.0 71 960.0 72 673.0 73 534.5 74 369.0 75 234.5 76 160.0 77 93.0 78 49.0 79 24.5 80 15.0 81 9.5 82 6.5 83 5.5 84 3.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022705692936465342 2 0.006343497361407169 3 0.004480724326708238 4 0.0 5 0.0662543057746971 6 1.5103565146207544E-4 7 0.0 8 1.5103565146207544E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 1.7620826003908805E-4 16-17 0.0 18-19 0.0010824221688115407 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.7620826003908805E-4 28-29 0.0022907073805081444 30-31 0.008885930827685438 32-33 2.7689869434713836E-4 34-35 0.0010320769516575154 36-37 0.024795019448357387 38-39 0.02356156162808377 40-41 0.026355721180132165 42-43 0.02658227465732528 44-45 0.02726193508890462 46-47 0.028948499863564462 48-49 0.028419875083447194 50-51 0.024190876842509087 52-53 0.020263949904495124 54-55 0.014574940366090281 56-57 0.15262152580242724 58-59 0.7788656819813461 60-61 0.3129458698294203 62-63 3.020713029241509E-4 64-65 0.14970150320749379 66-67 1.4450084227548299 68-69 2.774675953009788 70-71 2.2325334820866685 72-73 1.8992984897441758 74-75 1.5928219803190475 76-77 1.140998828970249 78-79 0.4307788505784162 80-81 1.7620826003908805E-4 82-83 1.258630428850629E-4 84-85 7.551782573103772E-5 86-87 0.0025676060748552826 88-89 5.0345217154025156E-5 90-91 0.230178332828203 92-93 0.17706412873070645 94-95 1.3196488320413071 96-97 1.29266379564675 98-99 1.0224610151810969 100-101 0.4149956250006293 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1986286.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.81082758421003 #Duplication Level Percentage of deduplicated Percentage of total 1 80.97588232891387 51.671380657696034 2 11.596102438696926 14.79913786729054 3 3.236358466411492 6.195441363026463 4 1.4011219161742203 3.576269960698046 5 0.7393097477489221 2.358798342246614 6 0.43596583477174083 1.6691604429135452 7 0.2986310672036479 1.3339126878434433 8 0.2026033892961538 1.0342631953882764 9 0.1546286830426962 0.8880285809889853 >10 0.8854536459238298 10.169252499873412 >50 0.05187393056675666 2.227330331966056 >100 0.01977569953843464 2.36263850072363 >500 0.0017394047464888752 0.7417349883908714 >1k 3.953192605656502E-4 0.40976544522586167 >5k 1.5812770422626008E-4 0.5628851357282614 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5973 0.30071198206099226 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5184 0.2609896057264664 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3493 0.17585584351900985 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.034521715402515E-5 7 0.0 0.0 0.0 0.0 5.034521715402515E-5 8 0.0 0.0 0.0 5.034521715402515E-5 5.034521715402515E-5 9 0.0 0.0 0.0 5.034521715402515E-5 5.034521715402515E-5 10-11 0.0 0.0 0.0 7.551782573103772E-5 5.034521715402515E-5 12-13 0.0 0.0 0.0 1.006904343080503E-4 1.006904343080503E-4 14-15 0.0 0.0 0.0 1.5103565146207544E-4 1.7620826003908805E-4 16-17 0.0 0.0 0.0 2.013808686161006E-4 2.517260857701258E-4 18-19 0.0 0.0 0.0 2.517260857701258E-4 2.517260857701258E-4 20-21 0.0 0.0 0.0 3.2724391150116346E-4 3.020713029241509E-4 22-23 0.0 0.0 0.0 4.027617372322012E-4 3.020713029241509E-4 24-25 2.5172608577012574E-5 0.0 0.0 8.055234744644024E-4 3.020713029241509E-4 26-27 5.034521715402515E-5 0.0 0.0 0.0017872552089678926 3.020713029241509E-4 28-29 5.034521715402515E-5 0.0 0.0 0.005689009538404842 3.020713029241509E-4 30-31 5.034521715402515E-5 1.006904343080503E-4 0.0 0.019760497732954872 3.020713029241509E-4 32-33 5.034521715402515E-5 1.006904343080503E-4 0.0 0.05417145365773106 3.020713029241509E-4 34-35 5.034521715402515E-5 1.006904343080503E-4 0.0 0.1057249560234528 3.020713029241509E-4 36-37 5.034521715402515E-5 1.006904343080503E-4 0.0 0.19546530560050263 3.020713029241509E-4 38-39 5.034521715402515E-5 1.006904343080503E-4 0.0 0.32658942367816113 3.020713029241509E-4 40-41 5.034521715402515E-5 1.006904343080503E-4 0.0 0.4620684030396428 3.020713029241509E-4 42-43 5.034521715402515E-5 1.006904343080503E-4 0.0 0.6014743093391385 3.5241652007817604E-4 44-45 5.034521715402515E-5 1.006904343080503E-4 0.0 0.774611511131831 3.775891286551886E-4 46-47 5.034521715402515E-5 1.006904343080503E-4 0.0 0.9765965223537798 4.027617372322012E-4 48-49 5.034521715402515E-5 1.006904343080503E-4 0.0 1.1786822240100367 4.027617372322012E-4 50-51 5.034521715402515E-5 1.006904343080503E-4 0.0 1.3802644734947536 4.027617372322012E-4 52-53 5.034521715402515E-5 1.2586304288506286E-4 0.0 1.5649307300157176 4.5310695438622635E-4 54-55 5.034521715402515E-5 2.013808686161006E-4 0.0 1.7611512138735308 4.5310695438622635E-4 56-57 5.034521715402515E-5 2.013808686161006E-4 0.0 1.9442315960541432 4.5310695438622635E-4 58-59 5.034521715402515E-5 2.013808686161006E-4 0.0 2.0692891154647417 4.5310695438622635E-4 60-61 5.034521715402515E-5 2.768986943471383E-4 0.0 2.1590546376503688 4.5310695438622635E-4 62-63 7.551782573103772E-5 3.020713029241509E-4 0.0 2.233490041212595 4.5310695438622635E-4 64-65 1.006904343080503E-4 3.020713029241509E-4 0.0 2.31366479953038 4.5310695438622635E-4 66-67 1.006904343080503E-4 3.020713029241509E-4 0.0 2.385658460060636 4.5310695438622635E-4 68-69 1.2586304288506286E-4 3.020713029241509E-4 0.0 2.4680232353246208 4.5310695438622635E-4 70-71 1.5103565146207544E-4 3.020713029241509E-4 0.0 2.6134957402911763 4.5310695438622635E-4 72-73 1.5103565146207544E-4 3.020713029241509E-4 0.0 2.7970795746433295 4.5310695438622635E-4 74-75 1.5103565146207544E-4 3.020713029241509E-4 0.0 3.012255032759633 4.782795629632389E-4 76-77 1.5103565146207544E-4 3.020713029241509E-4 0.0 3.3315695725590375 5.034521715402516E-4 78-79 1.7620826003908802E-4 3.020713029241509E-4 0.0 3.7720902226567574 5.034521715402516E-4 80-81 2.013808686161006E-4 3.020713029241509E-4 0.0 4.263585405122928 5.034521715402516E-4 82-83 2.013808686161006E-4 3.020713029241509E-4 0.0 4.729102455537621 6.544878230023269E-4 84-85 2.517260857701258E-4 3.020713029241509E-4 0.0 5.113890950245835 6.544878230023269E-4 86-87 3.020713029241509E-4 3.020713029241509E-4 0.0 5.453847029078391 6.544878230023269E-4 88-89 3.020713029241509E-4 3.020713029241509E-4 0.0 5.806339066982297 6.544878230023269E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3630 0.0 51.330196 1 GTATCAA 6155 0.0 38.089985 1 ATCAACG 7630 0.0 30.343409 3 TATCAAC 7825 0.0 29.769524 2 TCAACGC 7800 0.0 29.681328 4 CAACGCA 7875 0.0 29.338776 5 AACGCAG 8295 0.0 27.946537 6 ACGCAGA 9205 0.0 25.081388 7 CGCAGAG 9350 0.0 24.490856 8 GTACATG 6035 0.0 23.449116 1 TACATGG 5965 0.0 22.925394 2 ACATGGG 5975 0.0 21.938871 3 GCAGAGT 10335 0.0 21.88316 9 TGGTATC 1740 0.0 21.680538 2 GAGTACT 6705 0.0 21.11665 12-13 GTACTTT 6930 0.0 20.363052 14-15 CAGAGTA 9935 0.0 20.132103 10-11 GTGGTAT 1970 0.0 20.113865 1 CATGGGG 3530 0.0 19.63537 4 AGAGTAC 9465 0.0 19.48904 10-11 >>END_MODULE