FastQCFastQC Report
Fri 27 May 2016
SRR939740_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939740_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2154707
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56180.2607315054900736No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45900.2130220025274898No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28350.13157241332580255No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21600.10024564824823051No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37450.046.3480531
GTATCAA65500.040.862781
ATCAACG78600.033.5774543
TCAACGC79950.032.95244
TATCAAC80400.032.823432
CAACGCA81500.032.2679755
AACGCAG84250.031.270566
ACGCAGA96550.027.1406617
CGCAGAG96850.026.6679868
TACATGG61800.025.2712522
GTACATG64800.024.2453481
GTGGTAT19000.023.767011
GCAGAGT103000.023.4770459
ACATGGG64600.023.1580523
GAGTACT64950.022.09164812-13
TGGTATC20450.021.8528162
AGAGTAC92350.020.52936710-11
CAGAGTA98700.020.44784510-11
GTACTTT70750.020.31833514-15
AGTACTT69650.019.7903712-13
Produced by FastQC (version 0.11.5)