##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939740_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2154707 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.860090954361777 31.0 30.0 33.0 26.0 34.0 2 29.937000715178446 31.0 30.0 33.0 25.0 34.0 3 29.880025451256248 31.0 30.0 34.0 25.0 34.0 4 33.51031114671275 35.0 33.0 37.0 28.0 37.0 5 33.364274585825356 35.0 33.0 37.0 27.0 37.0 6 33.33535046760418 35.0 33.0 37.0 26.0 37.0 7 33.31462282342797 35.0 33.0 37.0 26.0 37.0 8 33.15518861729228 35.0 33.0 37.0 26.0 37.0 9 34.44040419416654 37.0 33.0 39.0 27.0 39.0 10-11 34.235388384592426 37.0 33.0 39.0 26.5 39.0 12-13 34.12333393821062 37.0 33.0 39.0 26.0 39.0 14-15 34.70571636886129 38.0 33.0 40.0 25.0 41.0 16-17 34.48515668255591 38.0 33.0 40.0 25.0 41.0 18-19 34.507993894297464 38.0 33.0 40.0 25.0 41.0 20-21 34.623539302559465 38.0 33.0 40.0 25.0 41.0 22-23 34.53807130157372 38.0 33.0 40.0 24.5 41.0 24-25 34.35809625160172 38.0 33.0 40.0 24.0 41.0 26-27 34.23361227303759 38.0 33.0 40.0 23.0 41.0 28-29 34.1023043968391 38.0 32.5 40.0 22.0 41.0 30-31 33.902135417947775 38.0 32.0 40.0 21.0 41.0 32-33 33.60116943974285 38.0 32.0 40.0 19.0 41.0 34-35 33.32032638312309 37.0 31.0 40.0 18.0 41.0 36-37 33.0926852235594 37.0 31.0 40.0 16.5 41.0 38-39 32.802814025294396 37.0 31.0 40.0 16.0 41.0 40-41 32.50153872429058 37.0 30.5 40.0 14.5 41.0 42-43 32.183452088845485 37.0 30.0 39.5 11.5 41.0 44-45 31.781628546247816 36.0 30.0 39.0 9.0 41.0 46-47 31.395230302774344 36.0 29.5 39.0 8.5 40.0 48-49 31.046198392635283 36.0 29.0 39.0 7.5 40.0 50-51 30.32370317634834 35.0 28.0 38.5 6.0 40.0 52-53 30.44153033335855 35.0 28.0 38.5 2.0 40.0 54-55 30.507357844941332 35.0 28.0 39.0 2.0 40.0 56-57 30.260951953096175 35.0 28.0 39.0 2.0 40.0 58-59 29.7105439393848 35.0 27.0 38.0 2.0 40.0 60-61 29.21571123127182 34.0 26.0 38.0 2.0 40.0 62-63 28.759511386002828 34.0 26.0 38.0 2.0 40.0 64-65 28.21489139822723 34.0 25.0 37.5 2.0 39.5 66-67 27.666050418920065 33.0 23.5 37.0 2.0 39.0 68-69 27.11214726642648 32.5 22.5 36.0 2.0 39.0 70-71 26.557922956578317 32.0 21.5 36.0 2.0 39.0 72-73 26.066590956450227 32.0 20.0 35.5 2.0 38.0 74-75 25.472962681236936 31.0 18.5 35.0 2.0 38.0 76-77 24.80690808541486 31.0 15.0 35.0 2.0 37.0 78-79 24.147416794951702 30.5 9.5 34.0 2.0 36.0 80-81 23.459529532321564 30.0 6.5 34.0 2.0 36.0 82-83 22.69767049533881 29.0 2.0 34.0 2.0 35.0 84-85 21.928506520840187 29.0 2.0 34.0 2.0 35.0 86-87 21.125963065976023 28.5 2.0 33.5 2.0 35.0 88-89 20.265350927063402 26.5 2.0 33.0 2.0 35.0 90-91 19.322665912349102 25.5 2.0 32.0 2.0 34.0 92-93 18.339178598296662 24.5 2.0 32.0 2.0 34.0 94-95 16.97444408914994 20.0 2.0 31.5 2.0 34.0 96-97 14.457140112321536 2.0 2.0 30.5 2.0 34.0 98-99 13.084398249970878 2.0 2.0 29.5 2.0 34.0 100-101 11.832550086856358 2.0 2.0 27.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7342650629342806 1101 2 0.6550352798434709 1101 3 0.8570788933411038 1101 4 0.8550283051132439 1101 5 0.9024626680309709 1101 6 0.9840662496143082 1101 7 0.7380979545711384 1101 8 0.5258827030297866 1101 9 0.7877604379871812 1101 10-11 0.8082143331833933 1101 12-13 0.6851585500552702 1101 14-15 0.8596033331893125 1101 16-17 0.8990774586374357 1101 18-19 0.890868872750417 1101 20-21 0.7846847124162011 1101 22-23 0.7311603113233147 1101 24-25 0.7208898749745174 1101 26-27 0.9279448654169897 1101 28-29 1.2296353471168473 1101 30-31 1.0249982500556172 1101 32-33 1.1726215295968316 1101 34-35 0.8485389293308785 1101 36-37 0.9016766194388666 1101 38-39 0.8513542446175322 1101 40-41 1.0833452916762454 1101 42-43 0.961878790518746 1101 44-45 0.8887697570425104 1101 46-47 1.0545979285978468 1101 48-49 0.8545023930383344 1101 50-51 0.7688432685681938 1101 52-53 0.933029069306194 1101 54-55 1.0102876364282984 1101 56-57 0.8049987007087438 1101 58-59 0.4678509107741142 1101 60-61 0.6469687073411983 1101 62-63 0.7004127022572426 1101 64-65 0.6531159349858484 1101 66-67 0.41662063030108953 1101 68-69 0.3288572272569752 1101 70-71 0.20007509359023246 1101 72-73 0.3449415111748664 1101 74-75 0.3740179180680627 1101 76-77 -0.10085661973125681 1101 78-79 -0.2351882771793221 1101 80-81 -0.5663736597369571 1101 82-83 -0.5225433205703389 1101 84-85 -0.34208051019365726 1101 86-87 -0.4825221399400945 1101 88-89 -0.5825316343931703 1101 90-91 -0.8183888247462825 1101 92-93 -0.7237244935420009 1101 94-95 -0.41391694769488296 1101 96-97 1.1780062946011487 1101 98-99 0.8685369172823822 1101 100-101 0.3048960679190049 1102 1 0.9006998719632442 1102 2 0.9071668054268969 1102 3 0.8659536355950657 1102 4 0.9996716255649645 1102 5 1.1615580306506246 1102 6 0.9752806943166874 1102 7 1.042405556542981 1102 8 0.9707839402107581 1102 9 1.139242819082618 1102 10-11 0.942522848361385 1102 12-13 0.9196905873755341 1102 14-15 1.2120424470891678 1102 16-17 1.0278260845608855 1102 18-19 1.1127820225776759 1102 20-21 0.9811731787077989 1102 22-23 0.9454646189252784 1102 24-25 1.1895099351266438 1102 26-27 1.19983770280826 1102 28-29 1.4465555209503336 1102 30-31 1.240942701517433 1102 32-33 1.263919955127001 1102 34-35 1.2281446461721046 1102 36-37 1.261707292924136 1102 38-39 1.1408723875488036 1102 40-41 1.1395684929161618 1102 42-43 0.9604814570807108 1102 44-45 0.7974022009528738 1102 46-47 0.7731533388749092 1102 48-49 0.6546497862188225 1102 50-51 1.0207010456707906 1102 52-53 0.985395894830905 1102 54-55 0.7745987290666605 1102 56-57 0.6772571619478356 1102 58-59 0.6343183647894932 1102 60-61 0.6296899080622005 1102 62-63 0.5605417374335993 1102 64-65 0.5559798177677351 1102 66-67 0.5630299805079702 1102 68-69 0.2412528059736374 1102 70-71 0.46929701634152465 1102 72-73 0.13755004615322264 1102 74-75 0.15510383967230013 1102 76-77 0.09361511184416926 1102 78-79 0.0593356437011785 1102 80-81 -0.07643087594320619 1102 82-83 -0.290247144770472 1102 84-85 -0.2914364011414783 1102 86-87 -0.423350861724586 1102 88-89 -0.3799334348601846 1102 90-91 -0.5111753922518503 1102 92-93 -0.9009643663417712 1102 94-95 -0.3120332252959024 1102 96-97 0.7971001124515134 1102 98-99 0.6098004361473421 1102 100-101 0.3685441899574844 1103 1 0.8507996748952813 1103 2 0.7586945580019169 1103 3 0.6761309544640604 1103 4 0.7247926939871832 1103 5 0.7227108549742027 1103 6 0.9734009561095718 1103 7 0.7064962543344606 1103 8 0.8019050703885497 1103 9 1.0649379605684501 1103 10-11 0.8134470332406636 1103 12-13 0.8078502881401661 1103 14-15 1.0143620034303424 1103 16-17 0.958088812236987 1103 18-19 0.7994497763116684 1103 20-21 0.6962543132676942 1103 22-23 0.8913150249258948 1103 24-25 0.8439956317316515 1103 26-27 1.112953529620242 1103 28-29 1.085694639765947 1103 30-31 1.0354283938926017 1103 32-33 1.1510244180671023 1103 34-35 1.0089097089280372 1103 36-37 0.9456633001131607 1103 38-39 0.7942174036835112 1103 40-41 1.0204935914395676 1103 42-43 0.7690630710922974 1103 44-45 0.8830748439519311 1103 46-47 0.5755177314559496 1103 48-49 0.6020952626017539 1103 50-51 0.9079540815283593 1103 52-53 0.9254723659988748 1103 54-55 0.8029961422694107 1103 56-57 0.7203871925422263 1103 58-59 0.48229358557624735 1103 60-61 0.7746941146320658 1103 62-63 0.810429646833942 1103 64-65 0.6498857268034612 1103 66-67 0.6513031254191226 1103 68-69 0.4816921122977931 1103 70-71 0.46728953714913146 1103 72-73 0.31850889875154564 1103 74-75 0.39892405227221417 1103 76-77 0.35935284352803976 1103 78-79 0.3577190223148854 1103 80-81 0.19796691202283156 1103 82-83 0.1609616148772517 1103 84-85 -0.04251363410254427 1103 86-87 -0.08333428500533913 1103 88-89 -0.11833713359618159 1103 90-91 -0.2746851646487123 1103 92-93 -0.46701512688706615 1103 94-95 -0.3180003577941797 1103 96-97 1.2201759292641725 1103 98-99 1.1835880917153485 1103 100-101 0.5390347322602658 1104 1 0.5907705788330482 1104 2 0.6272597552923145 1104 3 0.7800388219588825 1104 4 0.8366554952495164 1104 5 0.7914214069450978 1104 6 1.063289697591813 1104 7 0.9852926874285899 1104 8 0.9664177992876262 1104 9 0.9998656726832635 1104 10-11 0.9191237805119599 1104 12-13 0.8970069169166166 1104 14-15 1.000289170308065 1104 16-17 1.024793984702761 1104 18-19 0.9992468199708355 1104 20-21 0.9358074952411997 1104 22-23 0.8863862198242813 1104 24-25 1.111318844258534 1104 26-27 1.3356279175476402 1104 28-29 1.2375610717545271 1104 30-31 1.087697660611532 1104 32-33 1.2902027809487677 1104 34-35 1.2794110632266111 1104 36-37 1.1257938052489465 1104 38-39 1.0005707977215792 1104 40-41 1.0085291549684783 1104 42-43 0.653481916194707 1104 44-45 0.8862821948534503 1104 46-47 0.8726273467246024 1104 48-49 0.7209277391339448 1104 50-51 0.9116347459638234 1104 52-53 0.7646082657154523 1104 54-55 0.7860724352602233 1104 56-57 0.8491934876436353 1104 58-59 0.6540834708000709 1104 60-61 0.9252816264413504 1104 62-63 0.6632479823783726 1104 64-65 0.5931744738134519 1104 66-67 0.6369692945883507 1104 68-69 0.47325412861972893 1104 70-71 0.7287395160431807 1104 72-73 0.8573218648413174 1104 74-75 0.5822870344427074 1104 76-77 0.39053154812511437 1104 78-79 0.21798066073978006 1104 80-81 0.3800220789608595 1104 82-83 0.3247453718371922 1104 84-85 0.19392830442888354 1104 86-87 0.3020739015913776 1104 88-89 0.6574538998919266 1104 90-91 0.2538958118533259 1104 92-93 0.327209694214865 1104 94-95 0.42712580052056026 1104 96-97 1.4208106153885165 1104 98-99 1.6621740306286927 1104 100-101 1.6169499290481415 1105 1 0.7244532150341527 1105 2 0.764313855896777 1105 3 0.8111555924258873 1105 4 0.6858777294780865 1105 5 0.8439705320097701 1105 6 0.902092195348807 1105 7 0.8559075734446893 1105 8 0.9662591927007256 1105 9 0.9095031000721505 1105 10-11 0.990925698689793 1105 12-13 0.8224899609515433 1105 14-15 0.9113731920963204 1105 16-17 0.9688509856589036 1105 18-19 1.033898675442785 1105 20-21 0.9192966518716545 1105 22-23 0.9969369551039549 1105 24-25 0.9851813792133797 1105 26-27 1.0361924956009148 1105 28-29 0.9562931645592343 1105 30-31 1.0780856921380177 1105 32-33 0.9577000344966464 1105 34-35 1.183517827766849 1105 36-37 1.2862909826656548 1105 38-39 1.345444141664153 1105 40-41 1.0255307578159503 1105 42-43 1.0579101916356137 1105 44-45 0.9914655809055688 1105 46-47 1.098672840414718 1105 48-49 1.1392906776658087 1105 50-51 1.0831194714332213 1105 52-53 0.9232943605634212 1105 54-55 1.0717276053028577 1105 56-57 1.038308716659266 1105 58-59 0.9856887086239965 1105 60-61 1.2463249958264733 1105 62-63 0.9192677674756595 1105 64-65 1.0437359820618113 1105 66-67 1.0744596758663043 1105 68-69 0.9708566109867647 1105 70-71 1.0200492667259056 1105 72-73 0.8643486482751754 1105 74-75 1.0325111711317092 1105 76-77 0.9341846855475922 1105 78-79 0.7485950064702074 1105 80-81 0.6025096687628881 1105 82-83 0.5283535591296733 1105 84-85 0.3649843828063233 1105 86-87 0.3591928405685678 1105 88-89 0.6856477044525917 1105 90-91 0.46159881191226404 1105 92-93 0.8040088250098414 1105 94-95 0.9684828261988621 1105 96-97 2.1948967140021107 1105 98-99 2.2808003993523744 1105 100-101 1.6357042881711106 1106 1 1.0501856089429324 1106 2 1.0574189617433483 1106 3 1.119955890435591 1106 4 1.0550103676705191 1106 5 1.157993527536398 1106 6 1.2760567374417136 1106 7 1.2311023519085822 1106 8 1.3041195234493443 1106 9 1.382639964083495 1106 10-11 1.3521986965284896 1106 12-13 1.2792005884946818 1106 14-15 1.5988140057901319 1106 16-17 1.6655649210753651 1106 18-19 1.420736823868225 1106 20-21 1.5290125511497124 1106 22-23 1.271258265744443 1106 24-25 1.3356612036595052 1106 26-27 1.6586753233723357 1106 28-29 1.3893658325999496 1106 30-31 1.4213985294179636 1106 32-33 1.3985471726808 1106 34-35 1.6406823738171497 1106 36-37 1.6070134970451235 1106 38-39 1.403102545888423 1106 40-41 1.5305827922978423 1106 42-43 1.4664780351925657 1106 44-45 1.7080833213011744 1106 46-47 1.5230282514007776 1106 48-49 1.2970268749836826 1106 50-51 1.514915124010166 1106 52-53 1.733533626482238 1106 54-55 1.721715141631929 1106 56-57 1.5650160298768014 1106 58-59 1.3630186569433178 1106 60-61 1.8523943841688641 1106 62-63 1.7288185741945554 1106 64-65 1.821217301073073 1106 66-67 1.7712115722477115 1106 68-69 1.8223936429296828 1106 70-71 1.8029872862561227 1106 72-73 1.764615622472256 1106 74-75 1.9629606612180908 1106 76-77 1.6357319228604048 1106 78-79 1.4868810377169233 1106 80-81 1.683443413866943 1106 82-83 1.543775711944388 1106 84-85 1.6476132301716255 1106 86-87 1.8053341891356105 1106 88-89 1.735079870789157 1106 90-91 1.7382174599298104 1106 92-93 1.8745925614140582 1106 94-95 2.361826279588499 1106 96-97 3.9913369003184 1106 98-99 3.978056327016196 1106 100-101 3.411298957978463 1107 1 0.6351786369099948 1107 2 0.44099079520862716 1107 3 0.5186987719062479 1107 4 0.16664660693442102 1107 5 0.5948446082612975 1107 6 0.525406771763727 1107 7 0.5033608361431803 1107 8 0.6367393096722225 1107 9 0.7200552652442269 1107 10-11 0.6465285083673109 1107 12-13 0.6195482095126721 1107 14-15 0.6674568391860589 1107 16-17 0.6859392821716597 1107 18-19 0.6761763250390018 1107 20-21 0.6316258078185868 1107 22-23 0.3039443042185823 1107 24-25 0.4583450656156458 1107 26-27 0.7349299903292064 1107 28-29 0.8015146298943705 1107 30-31 0.7679767321881243 1107 32-33 0.9685712578855004 1107 34-35 0.7064574653624547 1107 36-37 0.5982081662502665 1107 38-39 0.6058344347472868 1107 40-41 0.5384637008864743 1107 42-43 0.45873584972931525 1107 44-45 0.47982757326064274 1107 46-47 0.21127194488007106 1107 48-49 0.23642280752597244 1107 50-51 0.43553440317610637 1107 52-53 0.5513351519582592 1107 54-55 0.3187866486195112 1107 56-57 0.35826356215982713 1107 58-59 0.29104048909311686 1107 60-61 0.4303149245193545 1107 62-63 0.3431599144034543 1107 64-65 0.39369893819729285 1107 66-67 0.45678125789632773 1107 68-69 -0.028956156998084737 1107 70-71 0.09651465656794045 1107 72-73 0.14531134286384173 1107 74-75 0.14217060910124601 1107 76-77 0.14288573092978396 1107 78-79 0.027095197521180836 1107 80-81 -0.2235809990113431 1107 82-83 0.04341933701882894 1107 84-85 -0.17805844089192746 1107 86-87 -0.11639846270043464 1107 88-89 0.032742625304233997 1107 90-91 -0.0645864214475651 1107 92-93 -0.03564759701731646 1107 94-95 0.13156263389693024 1107 96-97 1.5459364090471919 1107 98-99 1.9258210908639928 1107 100-101 1.6338467277650448 1108 1 0.41096985419536836 1108 2 0.5778938132633265 1108 3 0.4507815755820701 1108 4 0.5816736111915475 1108 5 0.6795554649161133 1108 6 0.7141049149831176 1108 7 0.6614702236604799 1108 8 0.6389721471137122 1108 9 0.6959707451470365 1108 10-11 0.6686708819612406 1108 12-13 0.4151228589456011 1108 14-15 0.552146054366041 1108 16-17 0.6004280426737765 1108 18-19 0.5508772547534448 1108 20-21 0.42471148074844933 1108 22-23 0.5242938285199372 1108 24-25 0.41996920657737036 1108 26-27 0.6678290045041564 1108 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1.9161010519877202 2311 94-95 2.3197257053268707 2311 96-97 3.8626404712380715 2311 98-99 4.020360323708692 2311 100-101 3.545019804130705 2312 1 0.08762802572426409 2312 2 -0.36230474944682456 2312 3 -0.597790326162631 2312 4 -0.21506240139 2312 5 -1.7361203722882195 2312 6 -0.6376644464805423 2312 7 -0.2827938981900857 2312 8 0.21047869379566464 2312 9 0.11813131215901507 2312 10-11 0.15940377183056142 2312 12-13 0.17731315893977495 2312 14-15 0.4459878083325961 2312 16-17 -0.4065206196457112 2312 18-19 0.006430738488788279 2312 20-21 0.3581484523330616 2312 22-23 0.2834639612675929 2312 24-25 0.10531758415992698 2312 26-27 0.4887913440764393 2312 28-29 0.5898694776488114 2312 30-31 0.27335994932635543 2312 32-33 -0.4612973316178781 2312 34-35 -0.0955232391516958 2312 36-37 0.3044909313171118 2312 38-39 0.29046231262572775 2312 40-41 0.42637554441345316 2312 42-43 0.18356626424755262 2312 44-45 0.10611716728677578 2312 46-47 -0.12617268469687914 2312 48-49 0.2617215081245057 2312 50-51 0.21233883415776944 2312 52-53 0.2410050032262241 2312 54-55 0.2980073344847298 2312 56-57 0.0873113287286813 2312 58-59 0.15760866953284847 2312 60-61 0.372273161267362 2312 62-63 0.3683514019750156 2312 64-65 0.413620241793339 2312 66-67 0.3918928707417102 2312 68-69 0.39177308354771867 2312 70-71 0.22372260675084377 2312 72-73 0.30287017779927794 2312 74-75 0.4404036567305809 2312 76-77 0.388209456069891 2312 78-79 0.552959370256275 2312 80-81 0.5203963347252447 2312 82-83 0.6897397961438116 2312 84-85 0.36105981466839765 2312 86-87 0.39476251551530694 2312 88-89 0.3649991654520406 2312 90-91 0.5102440849675496 2312 92-93 0.619166584427564 2312 94-95 1.155124891889045 2312 96-97 2.5791497795123117 2312 98-99 3.1523022796747124 2312 100-101 2.7550901619882193 2313 1 -0.10855921254928802 2313 2 -0.6031782768127094 2313 3 -0.9248086141885139 2313 4 -0.038839240566431954 2313 5 -0.23733135512678416 2313 6 0.06615412860500669 2313 7 -0.008361452429731742 2313 8 0.14285529522575757 2313 9 -0.00626815782300838 2313 10-11 -0.7365490277027646 2313 12-13 -0.051238151972704316 2313 14-15 0.07208618239619824 2313 16-17 -0.36293165219851886 2313 18-19 -0.04680158940070811 2313 20-21 -2.2873626321390503 2313 22-23 -0.3372215740926592 2313 24-25 0.1171817480084556 2313 26-27 0.07681061235494724 2313 28-29 -0.016188643914539114 2313 30-31 0.3542924321719383 2313 32-33 0.5608100711937851 2313 34-35 0.26172872586391094 2313 36-37 -0.6231330485887057 2313 38-39 0.1352347998012604 2313 40-41 0.25855068288165484 2313 42-43 0.31442317342483506 2313 44-45 0.36847576734972165 2313 46-47 0.3571341544194091 2313 48-49 0.25831949028751566 2313 50-51 0.570863924816102 2313 52-53 0.3661142041699925 2313 54-55 0.7013556288652154 2313 56-57 0.6408315181239104 2313 58-59 0.5467769783064078 2313 60-61 0.48365090732781013 2313 62-63 0.5991295301134762 2313 64-65 0.5928639438205998 2313 66-67 0.7838891782563806 2313 68-69 0.5979872637337849 2313 70-71 0.9099688005040889 2313 72-73 0.8335197104252501 2313 74-75 0.8774989016032997 2313 76-77 0.8134281085332518 2313 78-79 0.727531336635014 2313 80-81 0.7996660966381199 2313 82-83 0.9890384845297255 2313 84-85 0.9605234821763311 2313 86-87 1.0548893870661296 2313 88-89 1.3039690181735963 2313 90-91 1.5942301850254843 2313 92-93 1.6224063293614215 2313 94-95 1.9864538859173244 2313 96-97 3.0759366958460443 2313 98-99 3.290868504860187 2313 100-101 2.6584364517621193 2314 1 -0.46364704955420066 2314 2 -0.31773276647294324 2314 3 -1.4569620303639432 2314 4 -0.46198201283285556 2314 5 -0.642809926858483 2314 6 -0.6671321858783514 2314 7 -0.39232081682649067 2314 8 -1.8820599799052466 2314 9 -1.7677500195269573 2314 10-11 -0.8261782389379775 2314 12-13 -0.5740580906595838 2314 14-15 -0.8089475721532793 2314 16-17 -0.7402191226180719 2314 18-19 -2.1700159003758372 2314 20-21 -1.3190751960742446 2314 22-23 -1.9083738929370426 2314 24-25 -1.9004940725142703 2314 26-27 -1.9966529726235436 2314 28-29 -3.221060349142732 2314 30-31 -1.7973842815742032 2314 32-33 -1.6248593512625007 2314 34-35 -2.045044009400435 2314 36-37 -3.4058886832724546 2314 38-39 -3.838928813671462 2314 40-41 -4.414961605003043 2314 42-43 -3.34011902936113 2314 44-45 -3.6428318955776717 2314 46-47 -3.5315073578361442 2314 48-49 -1.9420097090245783 2314 50-51 -1.5138765048607397 2314 52-53 -2.617915789545112 2314 54-55 -2.055955162365027 2314 56-57 -2.1465584709027787 2314 58-59 -3.9111020808585266 2314 60-61 -2.859832502360632 2314 62-63 -2.529515458034137 2314 64-65 -1.9972249137223912 2314 66-67 -2.9864801554838216 2314 68-69 -2.872249501099887 2314 70-71 -2.1806473013637984 2314 72-73 -1.646413782826059 2314 74-75 -2.1429670296023318 2314 76-77 -1.1772784271542598 2314 78-79 -0.6620783732290434 2314 80-81 -0.955983743612304 2314 82-83 -0.6756210902393427 2314 84-85 -0.62242878291298 2314 86-87 -0.1849070509528552 2314 88-89 0.04991192462561855 2314 90-91 0.4775203391570457 2314 92-93 0.5818938979951334 2314 94-95 0.9871306744355621 2314 96-97 2.6338415210713233 2314 98-99 3.006808703349792 2314 100-101 2.7318699258151913 2315 1 -2.291039890989939 2315 2 -1.7907796617754386 2315 3 -1.630911547576126 2315 4 -1.7703951219847092 2315 5 -1.9913733568008674 2315 6 -3.384623822489278 2315 7 -2.5399381041348654 2315 8 -1.4368060244767307 2315 9 -1.4284209149747369 2315 10-11 -3.800235608228128 2315 12-13 -2.020279312450171 2315 14-15 -1.3760163480478553 2315 16-17 -2.1887038395381992 2315 18-19 -1.508538566950108 2315 20-21 -1.084397569838579 2315 22-23 -1.2036360202425769 2315 24-25 -3.0378272143703278 2315 26-27 -4.436036848581338 2315 28-29 -6.136839293920669 2315 30-31 -8.566189043847729 2315 32-33 -7.089408120669663 2315 34-35 -6.318053061502361 2315 36-37 -3.941119805453809 2315 38-39 -6.4060091742192675 2315 40-41 -4.346366890234947 2315 42-43 -4.123172915114743 2315 44-45 -3.389240809623928 2315 46-47 -2.343277720368995 2315 48-49 -3.037342012193495 2315 50-51 -2.748466150351568 2315 52-53 -2.3358497303039094 2315 54-55 -3.5929539356441786 2315 56-57 -3.4908795173371985 2315 58-59 -2.299253923726379 2315 60-61 -2.596856386158418 2315 62-63 -3.3298522896544362 2315 64-65 -2.4405020680973912 2315 66-67 -3.0086670624878593 2315 68-69 -2.4188274258438938 2315 70-71 -2.63807432691954 2315 72-73 -1.8911230925665024 2315 74-75 -2.356337765014903 2315 76-77 -1.9097107179870818 2315 78-79 -1.7117764312445694 2315 80-81 -1.5963453851764555 2315 82-83 -1.1015317013851167 2315 84-85 -0.9980872556465705 2315 86-87 -1.5543231698184918 2315 88-89 -2.269063152923721 2315 90-91 -1.8035558062468375 2315 92-93 -1.076094367481705 2315 94-95 -0.39970437951447835 2315 96-97 1.0910677459709799 2315 98-99 1.025306263580072 2315 100-101 0.9388428016542196 2316 1 -3.7812667185954822 2316 2 -2.3379094167526553 2316 3 -2.456333757805215 2316 4 -3.304470559336032 2316 5 -2.9017551042060283 2316 6 -6.198964906841663 2316 7 -3.6453796087523074 2316 8 -5.003269610090516 2316 9 -6.723599193010923 2316 10-11 -5.969844209318158 2316 12-13 -4.019749212493082 2316 14-15 -5.258389980257412 2316 16-17 -4.813427554975281 2316 18-19 -2.991761910918484 2316 20-21 -3.6316870337274416 2316 22-23 -4.34449119788264 2316 24-25 -3.273149243358894 2316 26-27 -4.158426550826594 2316 28-29 -3.720297761305673 2316 30-31 -3.4312408328569504 2316 32-33 -3.5278161956901783 2316 34-35 -5.5184026106425925 2316 36-37 -5.818285763272982 2316 38-39 -5.683335940161221 2316 40-41 -5.074053281419189 2316 42-43 -4.38783838529368 2316 44-45 -3.5913333627702464 2316 46-47 -3.7977385885604313 2316 48-49 -4.324804582437938 2316 50-51 -5.420449586317453 2316 52-53 -3.4265194537833388 2316 54-55 -3.750980946494689 2316 56-57 -3.1295136418941993 2316 58-59 -3.507824494482062 2316 60-61 -3.455702050664957 2316 62-63 -2.5753499962905053 2316 64-65 -2.9947235668997862 2316 66-67 -3.587675341271943 2316 68-69 -2.909199413978449 2316 70-71 -2.7332372375530305 2316 72-73 -2.6101016840287343 2316 74-75 -3.495276004651984 2316 76-77 -3.4644855941587913 2316 78-79 -3.468778136746078 2316 80-81 -3.8385481525590244 2316 82-83 -2.824394711012264 2316 84-85 -2.382458757972909 2316 86-87 -2.3046127903988314 2316 88-89 -2.283512175498714 2316 90-91 -1.7113585025901976 2316 92-93 -1.4843521587872033 2316 94-95 -1.3209768306324534 2316 96-97 0.24234624706081576 2316 98-99 0.5102253023695091 2316 100-101 0.32074700605058837 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20976.0 3 6955.0 4 7097.0 5 7716.0 6 8544.0 7 10053.0 8 12505.0 9 14794.0 10 17578.0 11 20376.0 12 23096.0 13 25662.0 14 27083.0 15 29795.0 16 31943.0 17 33992.0 18 32728.0 19 33213.0 20 35911.0 21 38897.0 22 42837.0 23 47591.0 24 53141.0 25 58720.0 26 65303.0 27 73343.0 28 82269.0 29 92132.0 30 103396.0 31 116594.0 32 133300.0 33 151205.0 34 171409.0 35 186060.0 36 180923.0 37 128399.0 38 28899.0 39 272.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.58722970646576 18.91428621876439 14.079077165124565 23.41940690964528 2 15.783829693913297 19.909295598220666 40.2689072819066 24.03796742595943 3 19.556585488936708 24.010247083331205 29.504741343858605 26.928426083873486 4 13.112815891607069 16.55814143708751 37.14839289556714 33.18064977573828 5 14.041164961916136 36.83241705896842 34.40703958955865 14.719378389556795 6 32.23077470021791 36.00052606801799 17.558728383925555 14.209970847838543 7 28.45290784763086 32.22463020119973 20.987775388389245 18.334686562780163 8 26.048296731309872 35.25059988650607 19.862134159859124 18.838969222324934 9 26.200318659621697 16.932464239102547 20.24083528235027 36.62638181892548 10-11 24.930093192809398 26.473469108894736 27.522960084160058 21.073477614135804 12-13 25.88468388411315 23.707413404622386 27.84486172253785 22.563040988726613 14-15 22.821892420910576 25.373419745077303 25.913392457158153 25.891295376853975 16-17 22.43467632686351 28.367982860741197 26.717376503693448 22.479964308701845 18-19 22.274613756717045 27.037866459920362 28.868742589227903 21.81877719413469 20-21 22.835864578996322 26.394626045851847 29.091994512786645 21.67751486236519 22-23 22.51938827112772 26.21591866865849 28.991391386736577 22.273301673477217 24-25 22.333857661249436 26.398964863208086 29.11057117635185 22.156606299190624 26-27 22.20833074612343 26.624866656945734 28.864355549850128 22.302447047080708 28-29 22.19018282919546 26.87011254873643 28.904548113160377 22.035156508907736 30-31 22.330475058448858 26.60742205714395 28.846816203025504 22.215286681381695 32-33 22.05326280062393 26.69563512579163 28.79570887256739 22.45539320101706 34-35 22.582450374468486 26.776967066814255 28.65683072229534 21.983751836421916 36-37 22.590435948603407 26.512085713063584 28.634501330665184 22.262977007667832 38-39 22.200514475912538 26.78476269659034 28.632463132481266 22.38225969501585 40-41 22.635948947107316 26.5930084262101 28.66582914456044 22.105213482122142 42-43 22.36833387230151 26.59248821728779 28.578084166912905 22.461093743497802 44-45 22.435417405304243 26.791637363339937 28.56850585000959 22.204439381346226 46-47 22.569014658311836 26.938883045799873 28.045029016885998 22.447073279002293 48-49 22.42440466485892 26.955205753415928 28.118838869420838 22.50155071230432 50-51 22.3506779593992 27.25343283096026 28.030789056811813 22.365100152828727 52-53 22.53115409214971 27.10692773572144 27.929738713018004 22.43217945911084 54-55 22.31452199967755 27.095467454324524 28.033207446735496 22.55680309926243 56-57 22.271001136830904 27.253334672970404 27.95571383119457 22.519950359004127 58-59 22.317993718043112 26.98957704932215 27.918009904338064 22.774419328296666 60-61 22.155080471283473 27.074037207985118 28.099648185905536 22.671234134825873 62-63 22.15491152519447 27.061263842097272 28.176094516959015 22.607730115749238 64-65 21.943182256706436 27.112149067768517 28.04933218089768 22.895336494627365 66-67 21.981438398580682 27.11949274998466 27.89059143834279 23.00847741309187 68-69 22.129110909529654 26.982951694996814 28.010838903816353 22.87709849165718 70-71 22.03137920989598 26.91525028113051 27.840417837704756 23.212952671268752 72-73 22.07947120286371 27.055596073557535 27.776222175915933 23.088710547662814 74-75 22.04323033882962 27.34935762950448 27.519265532675814 23.08814649899009 76-77 22.15997491290236 27.143081134800344 27.583128926837986 23.11381502545931 78-79 22.243030219150775 27.25008402931315 27.50274185613484 23.004143895401242 80-81 22.273821949460128 27.23543657533096 27.354536727160394 23.136204748048513 82-83 22.317055072474556 27.294717454604832 27.342844635040585 23.04538283788002 84-85 22.270952592820347 27.253645261456644 27.2832525084176 23.19214963730541 86-87 22.36860128422569 27.194435936738014 27.261784413711275 23.17517836532502 88-89 22.395217045090902 27.1771254269831 27.0974033101888 23.330254217737195 90-91 22.33412320897114 27.33473602346882 27.045678285645046 23.285462481914994 92-93 22.463757666535646 27.094263791623735 27.07454070561201 23.36743783622861 94-95 22.34185968295879 26.99543980547884 27.110526220304425 23.552174291257945 96-97 22.536796550007146 26.82800235383992 27.094360600341073 23.540840495811864 98-99 22.526310399880842 26.82141045154744 26.814591391693316 23.837687756878406 100-101 22.56275876965532 26.9471441885235 26.858486341244962 23.631610700576218 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2778.0 1 1617.5 2 454.0 3 463.0 4 431.5 5 400.5 6 565.5 7 1025.5 8 1582.5 9 1857.5 10 1653.0 11 1398.0 12 1372.5 13 1427.0 14 1613.0 15 1825.0 16 2039.0 17 2263.5 18 2497.5 19 2853.5 20 3425.0 21 4215.5 22 5166.0 23 6465.5 24 7999.0 25 9835.5 26 12311.5 27 15502.0 28 18822.0 29 21916.5 30 25315.0 31 29587.0 32 34394.5 33 38171.5 34 41374.5 35 45075.0 36 49329.0 37 53506.0 38 56661.0 39 59352.5 40 62848.5 41 66847.0 42 70027.0 43 72662.0 44 75611.0 45 79174.5 46 81272.5 47 82238.5 48 82912.5 49 81793.0 50 79701.5 51 77675.5 52 76040.0 53 74757.5 54 72914.0 55 68468.5 56 62532.0 57 56645.0 58 50851.0 59 44593.5 60 37265.5 61 30681.0 62 25013.5 63 19339.0 64 14382.5 65 10737.5 66 7868.5 67 5857.5 68 4517.0 69 3326.5 70 2437.5 71 1772.0 72 1296.5 73 991.0 74 741.0 75 519.0 76 378.0 77 275.5 78 183.0 79 128.0 80 88.5 81 53.5 82 33.5 83 30.0 84 23.0 85 13.0 86 8.0 87 8.0 88 5.0 89 4.0 90 3.0 91 3.0 92 2.5 93 0.5 94 1.5 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.11862401709373943 2 0.1132868645249679 3 0.12437885986354524 4 0.13830186656468837 5 0.13352163426396257 6 0.13431060464369401 7 0.14220030844100845 8 0.14155056812828842 9 0.14828002136717428 10-11 0.14352299407761704 12-13 0.14345337904411135 14-15 0.1317580534151511 16-17 0.13561008526913404 18-19 0.15213205322115722 20-21 0.18046537185798348 22-23 0.19698733981000663 24-25 0.21594583393472988 26-27 0.2184751801521042 28-29 0.22042440109026423 30-31 0.2204708111126014 32-33 0.22195593182738999 34-35 0.19146454715188654 36-37 0.18113831718187207 38-39 0.19348338312355232 40-41 0.18183446751692922 42-43 0.18529201418104643 44-45 0.1888655859010065 46-47 0.2085434353719554 48-49 0.22687539419512723 50-51 0.24328133709130753 52-53 0.26321444168511077 54-55 0.2575988289823164 56-57 0.22606321880422722 58-59 0.2199835058780614 60-61 0.19870451063648095 62-63 0.18872635583399505 64-65 0.18099908711486062 66-67 0.17705423521620342 68-69 0.18266984791899782 70-71 0.18635944469480073 72-73 0.22142221657051286 74-75 0.23265344197610163 76-77 0.2503820705088905 78-79 0.23945251024849318 80-81 0.24390787239285897 82-83 0.24105365601912462 84-85 0.22720026435148724 86-87 0.21791825988405847 88-89 0.2199370958557242 90-91 0.22232721200608715 92-93 0.22935833039016443 94-95 0.23288549208778736 96-97 8.750980991847152 98-99 0.2931721111037371 100-101 0.30449615655400014 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2154707.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.11034739493125 #Duplication Level Percentage of deduplicated Percentage of total 1 84.38570357319456 58.31925289109168 2 9.631916331695404 13.313301675247624 3 2.675028216931671 5.546163880901739 4 1.1057926798697415 3.056868650102793 5 0.5789228556439645 2.000477983421001 6 0.3579912825361025 1.4844541140256218 7 0.2362351811307256 1.1428406804394269 8 0.1765403792842181 0.976061355325219 9 0.11770910836433313 0.7321425633546004 >10 0.6828582575372991 8.468883328949515 >50 0.03458511731573712 1.6162704482261825 >100 0.015087680429160522 1.9401898995352802 >500 0.0012220020503806133 0.5859629693836719 >1k 3.3944501399459743E-4 0.5535424063406421 >5k 6.788900279891949E-5 0.2635871536550032 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5618 0.2607315054900736 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4590 0.2130220025274898 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2835 0.13157241332580255 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2160 0.10024564824823051 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 4.641002233714375E-5 6 0.0 0.0 0.0 0.0 4.641002233714375E-5 7 0.0 0.0 0.0 0.0 4.641002233714375E-5 8 0.0 0.0 0.0 0.0 4.641002233714375E-5 9 0.0 0.0 0.0 0.0 4.641002233714375E-5 10-11 0.0 4.641002233714375E-5 0.0 0.0 4.641002233714375E-5 12-13 0.0 4.641002233714375E-5 0.0 0.0 1.3923006701143124E-4 14-15 0.0 4.641002233714375E-5 0.0 4.641002233714375E-5 1.624350781800031E-4 16-17 0.0 4.641002233714375E-5 0.0 6.961503350571562E-5 2.3205011168571875E-4 18-19 0.0 4.641002233714375E-5 0.0 2.088451005171469E-4 3.2487015636000627E-4 20-21 0.0 4.641002233714375E-5 0.0 3.016651451914344E-4 3.2487015636000627E-4 22-23 0.0 4.641002233714375E-5 0.0 5.105102457085812E-4 4.176902010342938E-4 24-25 0.0 4.641002233714375E-5 0.0 9.28200446742875E-4 4.176902010342938E-4 26-27 0.0 4.641002233714375E-5 0.0 0.001693965815305747 4.4089521220286564E-4 28-29 0.0 4.641002233714375E-5 0.0 0.005406767602277247 4.641002233714375E-4 30-31 0.0 4.641002233714375E-5 0.0 0.01684683810838318 4.873052345400094E-4 32-33 0.0 4.641002233714375E-5 0.0 0.04815039817478664 5.105102457085812E-4 34-35 0.0 4.641002233714375E-5 0.0 0.0985980924552619 5.105102457085812E-4 36-37 0.0 6.961503350571562E-5 0.0 0.18090626707018634 5.105102457085812E-4 38-39 0.0 9.28200446742875E-5 0.0 0.3051226918555516 5.105102457085812E-4 40-41 0.0 9.28200446742875E-5 0.0 0.4334464036177541 5.105102457085812E-4 42-43 0.0 9.28200446742875E-5 0.0 0.5672232930045709 5.105102457085812E-4 44-45 0.0 9.28200446742875E-5 0.0 0.7386155054956428 5.337152568771532E-4 46-47 0.0 9.28200446742875E-5 0.0 0.938225011567698 5.56920268045725E-4 48-49 0.0 9.28200446742875E-5 0.0 1.144076665644099 5.56920268045725E-4 50-51 0.0 9.28200446742875E-5 0.0 1.3628767159525634 5.56920268045725E-4 52-53 0.0 9.28200446742875E-5 0.0 1.5730909121286558 6.497403127200125E-4 54-55 0.0 9.28200446742875E-5 0.0 1.8020779623401233 6.497403127200125E-4 56-57 0.0 9.28200446742875E-5 0.0 2.0661741944496397 6.729453238885845E-4 58-59 4.641002233714375E-5 9.28200446742875E-5 0.0 2.355958373922765 6.961503350571563E-4 60-61 1.3923006701143124E-4 9.28200446742875E-5 0.0 2.653423412092688 6.961503350571563E-4 62-63 1.85640089348575E-4 9.28200446742875E-5 0.0 2.9457137327720195 7.425603573943E-4 64-65 1.85640089348575E-4 1.3923006701143124E-4 0.0 3.239419559132634 7.425603573943E-4 66-67 1.85640089348575E-4 1.3923006701143124E-4 0.0 3.5756601709652402 7.425603573943E-4 68-69 1.85640089348575E-4 1.3923006701143124E-4 0.0 3.948959185634056 7.425603573943E-4 70-71 1.85640089348575E-4 1.3923006701143124E-4 0.0 4.337457482618287 7.425603573943E-4 72-73 1.85640089348575E-4 1.3923006701143124E-4 0.0 4.731757032394659 7.657653685628719E-4 74-75 1.85640089348575E-4 1.3923006701143124E-4 0.0 5.116588937614256 7.889703797314437E-4 76-77 1.85640089348575E-4 1.3923006701143124E-4 0.0 5.520379336958575 7.889703797314437E-4 78-79 1.85640089348575E-4 1.3923006701143124E-4 0.0 5.9697907882603065 7.889703797314437E-4 80-81 1.85640089348575E-4 1.624350781800031E-4 0.0 6.446909022897312 8.121753909000156E-4 82-83 1.85640089348575E-4 1.85640089348575E-4 0.0 6.929341205091922 8.817904244057313E-4 84-85 1.85640089348575E-4 1.85640089348575E-4 0.0 7.391399387480526 8.817904244057313E-4 86-87 1.85640089348575E-4 1.85640089348575E-4 0.0 7.806815497420299 9.049954355743032E-4 88-89 1.85640089348575E-4 1.85640089348575E-4 0.0 8.231165536659972 9.28200446742875E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3745 0.0 46.348053 1 GTATCAA 6550 0.0 40.86278 1 ATCAACG 7860 0.0 33.577454 3 TCAACGC 7995 0.0 32.9524 4 TATCAAC 8040 0.0 32.82343 2 CAACGCA 8150 0.0 32.267975 5 AACGCAG 8425 0.0 31.27056 6 ACGCAGA 9655 0.0 27.140661 7 CGCAGAG 9685 0.0 26.667986 8 TACATGG 6180 0.0 25.271252 2 GTACATG 6480 0.0 24.245348 1 GTGGTAT 1900 0.0 23.76701 1 GCAGAGT 10300 0.0 23.477045 9 ACATGGG 6460 0.0 23.158052 3 GAGTACT 6495 0.0 22.091648 12-13 TGGTATC 2045 0.0 21.852816 2 AGAGTAC 9235 0.0 20.529367 10-11 CAGAGTA 9870 0.0 20.447845 10-11 GTACTTT 7075 0.0 20.318335 14-15 AGTACTT 6965 0.0 19.79037 12-13 >>END_MODULE