FastQCFastQC Report
Fri 27 May 2016
SRR939740_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939740_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2154707
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65850.3056099970900916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57010.26458353734405654No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37250.17287733320586046No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36300.049.1450081
GTATCAA61450.038.99131
TCAACGC77400.030.7010964
ATCAACG77850.030.523633
TATCAAC77900.030.4488052
CAACGCA78650.030.1528515
AACGCAG81350.029.2103826
CGCGTAG350.00839990527.13932-33
ACGCAGA94450.025.0585357
CGCAGAG95350.024.8220088
TGGTATC18450.024.683832
GTGGTAT19200.023.7229251
GCAGAGT106100.021.7259089
GTACATG61150.021.1818731
TACATGG60950.020.781422
GAGTACT68100.020.2676312-13
AGAGTAC95550.019.73165510-11
ACATGGG62950.019.6653583
CAGAGTA101300.019.52466810-11
GTACTTT71150.019.19882614-15
Produced by FastQC (version 0.11.5)