##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939740_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2154707 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.385689562432386 31.0 31.0 34.0 30.0 34.0 2 31.61728624819987 31.0 31.0 34.0 30.0 34.0 3 31.658248198014856 31.0 31.0 34.0 30.0 34.0 4 35.46932459958593 37.0 35.0 37.0 33.0 37.0 5 35.19627308956624 37.0 35.0 37.0 32.0 37.0 6 35.2233004301745 37.0 35.0 37.0 32.0 37.0 7 35.12925423271006 37.0 35.0 37.0 32.0 37.0 8 35.09211275593387 37.0 35.0 37.0 32.0 37.0 9 36.68028182021964 39.0 35.0 39.0 32.0 39.0 10-11 36.54327664967905 38.0 35.0 39.0 32.0 39.0 12-13 36.461834254030826 38.0 35.0 39.0 32.0 39.0 14-15 37.49801457924442 39.0 36.0 41.0 32.0 41.0 16-17 37.413299812921196 39.0 36.0 41.0 32.0 41.0 18-19 37.38908491966657 39.0 36.0 41.0 32.0 41.0 20-21 37.299544903320964 39.0 36.0 40.5 31.0 41.0 22-23 37.17417727793152 39.0 36.0 40.0 31.0 41.0 24-25 37.01776691680122 39.0 36.0 40.0 31.0 41.0 26-27 36.71233095729489 39.0 35.5 40.0 30.0 41.0 28-29 36.47952413019496 38.0 35.0 40.0 30.0 41.0 30-31 36.21234534440181 38.0 35.0 40.0 30.0 41.0 32-33 36.194009440726745 38.0 35.0 40.0 30.0 41.0 34-35 36.03923572903416 38.0 35.0 40.0 29.5 41.0 36-37 36.08130038097987 38.0 35.0 40.0 30.0 41.0 38-39 35.980571372349004 38.0 35.0 40.0 29.0 41.0 40-41 35.896077981832335 38.0 35.0 40.0 29.0 41.0 42-43 35.674200482942695 38.0 34.0 40.0 28.0 41.0 44-45 35.43310018485112 38.0 34.0 40.0 27.0 41.0 46-47 35.1601851202971 38.0 34.0 40.0 26.5 41.0 48-49 34.851668463508034 38.0 33.0 40.0 26.0 41.0 50-51 34.62191680817856 38.0 33.0 40.0 24.5 41.0 52-53 34.37771748084542 38.0 33.0 40.0 24.0 41.0 54-55 34.10770141833669 37.0 33.0 40.0 24.0 41.0 56-57 33.73006770758159 37.0 32.5 40.0 23.0 41.0 58-59 33.35663596024889 36.5 32.0 39.5 22.5 41.0 60-61 33.03913757183692 36.0 31.0 39.0 22.0 40.5 62-63 32.74641424564918 36.0 31.0 39.0 21.5 40.0 64-65 32.30289686718426 35.0 31.0 39.0 19.5 40.0 66-67 31.376806220056835 35.0 29.5 38.0 14.5 40.0 68-69 29.447860660405336 34.0 26.0 37.0 7.5 39.5 70-71 28.7820471646493 33.5 25.0 36.5 7.5 39.0 72-73 29.35725947889899 33.5 26.5 36.0 7.5 39.0 74-75 29.469126428790553 34.0 27.5 36.0 8.0 39.0 76-77 29.04549296029576 32.5 27.5 35.0 8.0 37.5 78-79 29.576401339021963 34.0 29.0 35.0 7.5 37.5 80-81 29.38638501661711 34.0 29.0 35.0 7.0 37.0 82-83 28.933837872156168 33.5 28.5 35.0 3.5 36.5 84-85 28.5618464598667 33.0 27.5 35.0 2.0 36.0 86-87 28.15976255704372 33.0 27.0 35.0 2.0 36.0 88-89 27.685181558327884 33.0 26.0 35.0 2.0 35.0 90-91 27.161415217939144 32.5 26.0 35.0 2.0 35.0 92-93 26.648429925739322 32.0 25.0 34.0 2.0 35.0 94-95 25.805774520619277 32.0 23.5 34.0 2.0 35.0 96-97 25.258548610089445 32.0 20.5 34.0 2.0 35.0 98-99 24.55820791411547 31.0 17.0 34.0 2.0 35.0 100-101 23.07161112856644 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.18035331872266624 1101 2 0.25925127790337754 1101 3 0.22109963438116864 1101 4 0.09928361104054062 1101 5 0.1618944827610278 1101 6 0.15421436690974843 1101 7 0.028163687930117476 1101 8 0.139889641743423 1101 9 0.12129002410586054 1101 10-11 0.16063074965851598 1101 12-13 0.12265037704073478 1101 14-15 0.19422690158003064 1101 16-17 0.34363929537647664 1101 18-19 0.21365671154384103 1101 20-21 0.1553659652820656 1101 22-23 0.17003736898434596 1101 24-25 0.2437237313997187 1101 26-27 0.03586138768924485 1101 28-29 0.10721684649985974 1101 30-31 -0.015260857664976868 1101 32-33 0.07185860749847706 1101 34-35 0.1805681939731727 1101 36-37 0.3483019072074498 1101 38-39 0.3456989005412936 1101 40-41 0.29035779841643006 1101 42-43 0.08996557946689876 1101 44-45 0.007891692285340923 1101 46-47 -0.14507751031447214 1101 48-49 0.0519560616991086 1101 50-51 0.12405461474591561 1101 52-53 -0.06574909739480717 1101 54-55 -0.46146628173588766 1101 56-57 -0.13244835315214942 1101 58-59 -0.024652856286934366 1101 60-61 -0.09100625725708511 1101 62-63 -0.0840746194169526 1101 64-65 -0.15222135120529146 1101 66-67 -0.19046900272626743 1101 68-69 -9.006949817781884 1101 70-71 -6.792746445077146 1101 72-73 -1.6826178425834755 1101 74-75 -0.7281565614041838 1101 76-77 -0.48332172636091286 1101 78-79 -0.6025768466682351 1101 80-81 -0.6651317065344742 1101 82-83 -0.3825487999970214 1101 84-85 -0.21146128276851428 1101 86-87 -0.54131991089994 1101 88-89 -0.42325546047390006 1101 90-91 -0.8292999693885186 1101 92-93 -1.0867521712131776 1101 94-95 -0.763701279782893 1101 96-97 -1.3408608729735896 1101 98-99 -1.22382500025968 1101 100-101 -1.1114106614865946 1102 1 0.1588182959863964 1102 2 0.11475560667124896 1102 3 0.23848981067262898 1102 4 0.1834748176333747 1102 5 0.2331924132822536 1102 6 0.32084291219794636 1102 7 0.3600825474295135 1102 8 0.10619446825131718 1102 9 0.28839266847778333 1102 10-11 0.42959193925035777 1102 12-13 0.2972790776593186 1102 14-15 0.41664850240481854 1102 16-17 0.4422034707257154 1102 18-19 0.3671890697664466 1102 20-21 0.2524623964382755 1102 22-23 0.2696344487729547 1102 24-25 0.25197587188667114 1102 26-27 0.4111953053607138 1102 28-29 0.2399748315062098 1102 30-31 0.27987841054110874 1102 32-33 0.18592275041609696 1102 34-35 -0.0415809385663195 1102 36-37 -0.017906349883126893 1102 38-39 0.36976636343269575 1102 40-41 0.27758827458089996 1102 42-43 0.05312261849408628 1102 44-45 0.27997869266769726 1102 46-47 0.14520128213681716 1102 48-49 0.16445281853706462 1102 50-51 0.20445025491196134 1102 52-53 0.1996219971553117 1102 54-55 -0.04789996419277287 1102 56-57 -0.12458453864736185 1102 58-59 -0.28799377406765103 1102 60-61 -0.09340598380731535 1102 62-63 -0.03423980731552234 1102 64-65 -0.10143578054532298 1102 66-67 0.19542183414609582 1102 68-69 -4.6664233501657115 1102 70-71 0.08563284443139452 1102 72-73 0.45538937089169096 1102 74-75 0.15229024391050672 1102 76-77 -0.0868266030524758 1102 78-79 -0.011538727565671536 1102 80-81 -0.0904990799828802 1102 82-83 -0.18062099619808336 1102 84-85 -0.07854988766261783 1102 86-87 -0.28960423550291026 1102 88-89 -0.47247151139495713 1102 90-91 -0.6178276676993413 1102 92-93 -0.6175304191546225 1102 94-95 -0.23324756141225933 1102 96-97 -0.8667125017237112 1102 98-99 -0.36725298840677567 1102 100-101 -0.30597286021390957 1103 1 0.15821216078144573 1103 2 0.17294606678835578 1103 3 0.24433988251635697 1103 4 0.27457803869423003 1103 5 0.25257858823918156 1103 6 0.26233965586028063 1103 7 0.18863215005776368 1103 8 0.2899733511437148 1103 9 0.25848308963811917 1103 10-11 0.33568010844710017 1103 12-13 0.2611267926395797 1103 14-15 0.35754238898351076 1103 16-17 0.41530193829917295 1103 18-19 0.3306328356045398 1103 20-21 0.3144131789364124 1103 22-23 0.3986733585924682 1103 24-25 0.44241431109486484 1103 26-27 0.4163362455428299 1103 28-29 0.34439261812716637 1103 30-31 0.3419900324751737 1103 32-33 0.368773607739989 1103 34-35 0.2198297787331498 1103 36-37 0.2000775082245596 1103 38-39 0.42012835262207915 1103 40-41 0.446585156770297 1103 42-43 0.3992611396341559 1103 44-45 0.27674625356355875 1103 46-47 0.25311767016317077 1103 48-49 0.2952627598311963 1103 50-51 0.24650527320811477 1103 52-53 0.24341061289563015 1103 54-55 0.12319967419872313 1103 56-57 0.2984579682229551 1103 58-59 0.20007798844915925 1103 60-61 0.06842101260028954 1103 62-63 0.1779934857920935 1103 64-65 0.29063059485498144 1103 66-67 0.5968423815796662 1103 68-69 -3.729728736986143 1103 70-71 0.9201809860749997 1103 72-73 1.0362744763351088 1103 74-75 0.8288572599191575 1103 76-77 0.46953286769378266 1103 78-79 0.34551858118555856 1103 80-81 0.4443534828100617 1103 82-83 0.30831122028989455 1103 84-85 0.1999034650941134 1103 86-87 0.04254463857874313 1103 88-89 -0.03776946724310548 1103 90-91 -0.13519906700031825 1103 92-93 -0.23237601541105235 1103 94-95 -0.34023888475387665 1103 96-97 -0.4539447902457603 1103 98-99 -0.09297871159206039 1103 100-101 -0.24981462381779806 1104 1 0.18910034446352952 1104 2 0.18263030667257496 1104 3 0.20644464844130184 1104 4 0.21635450656292932 1104 5 0.2832374959559232 1104 6 0.2669861060401857 1104 7 0.15054093214383357 1104 8 0.19074271334194748 1104 9 0.25427320602236847 1104 10-11 0.18099766508140647 1104 12-13 0.14055338915407845 1104 14-15 0.2779556761852646 1104 16-17 0.28439070138988143 1104 18-19 0.2858162940660165 1104 20-21 0.23459279309950176 1104 22-23 0.3023919006737188 1104 24-25 0.23174895852496036 1104 26-27 0.23546797432129551 1104 28-29 0.21499994189246507 1104 30-31 0.21540645793096047 1104 32-33 0.08578016817336476 1104 34-35 0.026846314241396385 1104 36-37 0.1331750956132467 1104 38-39 0.2091837858971246 1104 40-41 0.09331989013177378 1104 42-43 0.12809017388610044 1104 44-45 0.006522857113381519 1104 46-47 0.1428814719481295 1104 48-49 -0.09977847215336766 1104 50-51 -0.051048132387983 1104 52-53 -0.18335738746619512 1104 54-55 -0.12036269640169195 1104 56-57 -0.035558481856448054 1104 58-59 0.02399655967672487 1104 60-61 -0.10588431263237652 1104 62-63 -0.05023566202996932 1104 64-65 -0.133726867104059 1104 66-67 0.191946366081865 1104 68-69 -4.219078711357486 1104 70-71 0.5470221034843945 1104 72-73 0.7184643821623595 1104 74-75 0.36230485434316506 1104 76-77 0.24744360459301262 1104 78-79 0.11131632335123243 1104 80-81 0.054399036489535035 1104 82-83 0.08668113727314974 1104 84-85 0.10248190888193065 1104 86-87 0.040989330934369406 1104 88-89 -0.06554672155977315 1104 90-91 -0.1983818696697206 1104 92-93 -0.18684275092332214 1104 94-95 0.21377884675831282 1104 96-97 -0.18776764346894126 1104 98-99 0.013179515206470427 1104 100-101 -0.015051425060033097 1105 1 0.19998607732010143 1105 2 0.22510392272266344 1105 3 0.1922875268213602 1105 4 0.2202236886686677 1105 5 0.2103005685768622 1105 6 0.23831965385055298 1105 7 0.19329881212838274 1105 8 0.2804833434787568 1105 9 0.32581480474402014 1105 10-11 0.29165297838159887 1105 12-13 0.3176985278928868 1105 14-15 0.31821545126270223 1105 16-17 0.34493390541484814 1105 18-19 0.27909147710824556 1105 20-21 0.33033253831388265 1105 22-23 0.2396436557835102 1105 24-25 0.35864210721157974 1105 26-27 0.26294610503113347 1105 28-29 0.4088857500615788 1105 30-31 0.2415499397937353 1105 32-33 0.23582210170840767 1105 34-35 0.24083821896120128 1105 36-37 0.052429505490323436 1105 38-39 0.32575389696675927 1105 40-41 0.10305465080159593 1105 42-43 0.04765422475154679 1105 44-45 0.11351722340741333 1105 46-47 0.20388760255318772 1105 48-49 0.37953697772449146 1105 50-51 0.25516726067802153 1105 52-53 0.31887190485782213 1105 54-55 0.5149325083174645 1105 56-57 0.11717648919734103 1105 58-59 0.14852012595503794 1105 60-61 0.3282160830859411 1105 62-63 0.40271108463171856 1105 64-65 0.3249072047834076 1105 66-67 0.815996670770236 1105 68-69 2.143653427289639 1105 70-71 2.279881336147774 1105 72-73 1.2840152566065228 1105 74-75 0.8965643887479047 1105 76-77 0.7099189233615562 1105 78-79 0.6612445513124854 1105 80-81 0.6148011318155717 1105 82-83 0.5122780710701491 1105 84-85 0.35690160933445725 1105 86-87 0.32720893577270616 1105 88-89 0.41986362130878163 1105 90-91 0.4514567404535974 1105 92-93 0.47045947587222514 1105 94-95 0.9971970499646652 1105 96-97 0.3217087419544775 1105 98-99 0.5926402896486636 1105 100-101 0.5978818881353831 1106 1 0.4629086672773717 1106 2 0.44094824878434125 1106 3 0.3852432191702171 1106 4 0.30515150799082136 1106 5 0.3144424432205071 1106 6 0.4060587267384008 1106 7 0.2925988893622744 1106 8 0.3178466114167122 1106 9 0.5113501500586821 1106 10-11 0.4598951147085657 1106 12-13 0.4752630943324263 1106 14-15 0.585223415322595 1106 16-17 0.5931878623346378 1106 18-19 0.6175036936135072 1106 20-21 0.5795418641077887 1106 22-23 0.6639073367324642 1106 24-25 0.6207869813091449 1106 26-27 0.7128050472472367 1106 28-29 0.6150080901862367 1106 30-31 0.6282535580006297 1106 32-33 0.8133785038520287 1106 34-35 0.7959222675486544 1106 36-37 0.8578137468076434 1106 38-39 0.7451425512193666 1106 40-41 0.7699401934379964 1106 42-43 0.7664536990190669 1106 44-45 0.8961180033170422 1106 46-47 0.8244423789351387 1106 48-49 1.0295804338886185 1106 50-51 1.0055392888882864 1106 52-53 0.89485523902637 1106 54-55 0.7332606428984363 1106 56-57 0.7259106727559157 1106 58-59 0.7578318968867492 1106 60-61 0.932662398216145 1106 62-63 1.0060029777826784 1106 64-65 1.0465757967413154 1106 66-67 1.4730986081129167 1106 68-69 3.0147427220179246 1106 70-71 3.4589715978590476 1106 72-73 2.3595060660740934 1106 74-75 1.7980640726933004 1106 76-77 1.3728607196314009 1106 78-79 1.2693786720257734 1106 80-81 1.3303940336958355 1106 82-83 1.3619287764235928 1106 84-85 1.4017398536839174 1106 86-87 1.3727145618458039 1106 88-89 1.3932062057902108 1106 90-91 1.3042421827138924 1106 92-93 1.498140439448413 1106 94-95 1.7305918162552452 1106 96-97 1.379017432133427 1106 98-99 1.6262117626179844 1106 100-101 1.7138308057962206 1107 1 0.10141533675039227 1107 2 0.05162179739363992 1107 3 0.05315679944665774 1107 4 0.02908292136420698 1107 5 0.10468829408358005 1107 6 0.06062140208562994 1107 7 0.04603288466003619 1107 8 0.004966169224786654 1107 9 0.13037100397126977 1107 10-11 0.06804892516972672 1107 12-13 -0.014167638580111941 1107 14-15 -0.04189839075682755 1107 16-17 -0.07725023964599131 1107 18-19 -0.06007147399034096 1107 20-21 0.08017807706213631 1107 22-23 0.106629036616944 1107 24-25 0.09264651696936 1107 26-27 -0.051272360256731986 1107 28-29 0.1268485872933809 1107 30-31 0.09898297854456217 1107 32-33 0.22216087357786307 1107 34-35 0.2078223199285958 1107 36-37 -0.07832697584417048 1107 38-39 0.03692033590672139 1107 40-41 0.0016508648340050058 1107 42-43 0.05486864380487333 1107 44-45 -0.02137043841565145 1107 46-47 0.03834745883248303 1107 48-49 0.1459248881925035 1107 50-51 0.06672814607283328 1107 52-53 0.0782017670018007 1107 54-55 0.08057312152192253 1107 56-57 0.09202970198105476 1107 58-59 0.33593421236705723 1107 60-61 0.1270421982156691 1107 62-63 0.2214818476197209 1107 64-65 0.13121723333886592 1107 66-67 0.6450351590255075 1107 68-69 2.156362290589005 1107 70-71 2.314447214877987 1107 72-73 1.3076176377353512 1107 74-75 0.658512196223846 1107 76-77 0.27615993823752305 1107 78-79 0.42119917386864003 1107 80-81 0.48557976721827956 1107 82-83 0.41518051823593893 1107 84-85 0.35724799888398096 1107 86-87 0.4168069897157558 1107 88-89 0.5721829553160411 1107 90-91 0.43073973472908733 1107 92-93 0.5137432033870972 1107 94-95 0.7928665406626187 1107 96-97 0.2549044828080298 1107 98-99 0.49261421753895007 1107 100-101 0.6666290664952399 1108 1 -0.17684168452197824 1108 2 -0.1950870846317727 1108 3 -0.1622147718485536 1108 4 -0.09025781227764895 1108 5 0.0060635829124464635 1108 6 -0.07087621280039968 1108 7 -0.1320677635083385 1108 8 -0.07556163448413855 1108 9 -0.17462172151386568 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0.3590617741073814 2316 74-75 0.01999078932472287 2316 76-77 -0.43371181147512416 2316 78-79 -0.6169739456313081 2316 80-81 -0.9207075601074699 2316 82-83 -0.5525384862794276 2316 84-85 -0.5974079960957859 2316 86-87 -0.7698841120556317 2316 88-89 -0.7343296194023843 2316 90-91 -0.5851178220169508 2316 92-93 -0.7135044397101531 2316 94-95 -0.23759989107728074 2316 96-97 -0.2832821353795687 2316 98-99 -0.6074770633504585 2316 100-101 -0.5611997641931659 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 20.0 4 36.0 5 77.0 6 205.0 7 583.0 8 1308.0 9 2252.0 10 3532.0 11 5511.0 12 7654.0 13 9657.0 14 11855.0 15 14768.0 16 17568.0 17 20700.0 18 19513.0 19 17277.0 20 17020.0 21 17707.0 22 19457.0 23 22131.0 24 26333.0 25 32491.0 26 40058.0 27 49681.0 28 59865.0 29 72638.0 30 85979.0 31 102162.0 32 121924.0 33 147539.0 34 182194.0 35 228604.0 36 287262.0 37 313675.0 38 180564.0 39 14902.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.5117392075327 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21.948366993749033 27.176641650117627 29.07177170724372 21.803219648889616 20-21 22.55401778524876 26.46234499632665 29.2188682730413 21.76476894538329 22-23 22.222302150594025 26.348779671667653 29.129320134941782 22.299598042796536 24-25 22.20396244405558 26.469046465978764 29.20719328000973 22.119797809955934 26-27 22.016738316338678 26.755664485540194 28.983495127157088 22.24410207096404 28-29 21.996587006957327 26.98633735352417 28.990623783187225 22.026451856331278 30-31 22.183062313140866 26.734674650461454 28.86870921122125 22.21355382517643 32-33 21.911320218363663 26.742424857721335 28.868720739326925 22.477534184588073 34-35 22.411157229043994 26.87133839315592 28.714075468493068 22.00342890930702 36-37 22.479599002011355 26.57938021290765 28.702603146669997 22.238417638410997 38-39 22.03941638187047 26.7781200338736 28.785822886418927 22.396640697837004 40-41 22.42954812636657 26.665382897358924 28.73006024811436 22.175008728160144 42-43 22.217003484320557 26.62425087108014 28.65149825783972 22.507247386759584 44-45 22.238574388419693 26.82549637186312 28.655429197907665 22.280500041809514 46-47 22.43237957739675 26.98840832571518 28.186340089336813 22.392872007551265 48-49 22.247851160258488 27.014632340354567 28.171232687402025 22.566283811984913 50-51 22.267412409270268 27.190874384828547 28.139310887196327 22.40240231870486 52-53 22.478703572115265 27.05291898765453 28.01997791171933 22.44839952851088 54-55 22.237815105758138 27.09424241805068 28.077337090485578 22.590605385705608 56-57 22.16299882404337 27.233238207587686 27.996774359781444 22.6069886085875 58-59 22.175669293440105 26.99230766552908 27.96964840844451 22.862374632586302 60-61 22.01485116231684 27.107235213179415 28.20442128064761 22.673492343856136 62-63 22.05705342989593 27.03991735140204 28.203323533663827 22.699705685038204 64-65 21.835515227153586 27.03096206073689 28.10518110534796 23.028341606761572 66-67 21.684998864671915 27.10784640695969 28.17233559879899 23.034819129569406 68-69 21.715211788775125 26.802453198618675 28.592696328124635 22.88963868448156 70-71 21.660230535800615 26.82155317525497 28.292019780181377 23.22619650876304 72-73 21.67916915414558 26.97554645494734 28.121739542824624 23.223544848082454 74-75 21.88067819397105 27.230656885510896 27.586424607511162 23.302240313006887 76-77 21.85918456263595 27.096327771939926 27.70248924524146 23.341998420182666 78-79 21.975762051038327 27.2012880369944 27.57701964149668 23.2459302704706 80-81 21.996369755824077 27.20051645815891 27.46415238300329 23.338961403013727 82-83 21.970718517812056 27.283855132938058 27.393173965920646 23.35225238332924 84-85 21.99869262451336 27.164566179444595 27.37322646300631 23.463514733035737 86-87 21.945285184840543 27.135797100406716 27.37829233694168 23.540625377811057 88-89 21.94006185070853 27.16049927031758 27.277661507645245 23.621777371328644 90-91 21.823205958356848 27.271837036266966 27.23213291205038 23.672824093325808 92-93 21.885389605492314 27.009499233633345 27.30147274670219 23.803638414172156 94-95 21.54788458791933 26.902431066373904 27.55970261034547 23.989981735361294 96-97 21.731296921174863 26.690717292429323 27.543644946293412 24.03434084010241 98-99 21.710662822602245 26.785589121749492 27.28481927218696 24.2189287834613 100-101 21.478472096354853 26.755139082508684 27.44850358169863 24.317885239437835 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 570.0 1 391.5 2 272.0 3 355.5 4 342.0 5 339.5 6 566.0 7 1148.5 8 1751.0 9 2060.5 10 1914.5 11 1556.5 12 1544.0 13 1665.5 14 1818.0 15 2024.5 16 2191.5 17 2374.5 18 2637.5 19 3028.0 20 3513.0 21 4214.0 22 5228.5 23 6347.5 24 7747.0 25 9733.0 26 12421.0 27 15825.5 28 19120.5 29 22112.5 30 25333.5 31 29895.0 32 35199.5 33 38648.5 34 41268.5 35 45054.5 36 49652.0 37 53774.0 38 56965.0 39 59638.0 40 62989.0 41 66803.5 42 69702.5 43 72393.5 44 75971.5 45 79251.5 46 80784.0 47 82130.0 48 82825.5 49 81854.0 50 80032.0 51 77955.5 52 76029.5 53 75017.0 54 73954.5 55 69536.0 56 62857.5 57 56622.0 58 51517.0 59 45406.5 60 37571.0 61 30465.5 62 24585.5 63 18674.5 64 13392.5 65 9779.0 66 7123.5 67 5446.5 68 4070.0 69 2864.5 70 2084.5 71 1479.5 72 1038.0 73 787.0 74 594.0 75 423.0 76 293.0 77 171.0 78 100.0 79 68.5 80 48.5 81 23.5 82 11.0 83 8.0 84 5.5 85 5.0 86 2.5 87 1.0 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021951940565468994 2 0.025432692240754777 3 4.641002233714375E-5 4 0.0 5 0.0 6 4.641002233714375E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.784601340228625E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.961503350571562E-5 26-27 4.641002233714375E-4 28-29 0.0 30-31 4.176902010342937E-4 32-33 0.0020652459940028968 34-35 0.003991261920994363 36-37 0.10105782363913052 38-39 0.09272722462961322 40-41 0.10170756395185053 42-43 0.10242691929807626 44-45 0.09685771661761901 46-47 0.09073159366911603 48-49 0.09168299912702749 50-51 0.09066197863561032 52-53 0.0709609241534928 54-55 0.060472259105298305 56-57 0.033229575993394926 58-59 0.013435701466603115 60-61 0.009073159366911603 62-63 0.0019724259493286095 64-65 0.005499587646951534 66-67 0.15531113975125155 68-69 0.4147663696270537 70-71 0.40003118753501055 72-73 0.18262343789666066 74-75 0.029377544139411994 76-77 0.030653819753683448 78-79 0.005128307468254384 80-81 0.0014387106924514561 82-83 2.3205011168571875E-5 84-85 3.9448518986572186E-4 86-87 0.0011834555695971658 88-89 1.1602505584285938E-4 90-91 8.817904244057313E-4 92-93 0.002761396329060053 94-95 0.07608923162174717 96-97 0.01682363309721461 98-99 0.018099908711486062 100-101 0.009862129746643047 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2154707.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.04324047242219 #Duplication Level Percentage of deduplicated Percentage of total 1 80.53481816432019 50.77175907936032 2 11.757476847256914 14.824588804611075 3 3.434855510091792 6.496332657322237 4 1.3868581334876982 3.4972812324239815 5 0.7524173308719692 2.37174133628898 6 0.45398968242170046 1.7172588432545903 7 0.3172124474971364 1.3998670425885298 8 0.2209867127893982 1.11453747804737 9 0.17018710814782145 0.9656232105842306 >10 0.8939035263645214 10.278674003365554 >50 0.05377771608744797 2.3118012378803035 >100 0.021159740336255 2.4664305543493468 >500 0.0016941514851475305 0.7222326287967527 >1k 5.156113215665464E-4 0.491348514453636 >5k 1.473175204475847E-4 0.5705233766730339 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6585 0.3056099970900916 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5701 0.26458353734405654 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3725 0.17287733320586046 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.641002233714375E-5 0.0 0.0 4.641002233714375E-5 6 0.0 4.641002233714375E-5 0.0 0.0 4.641002233714375E-5 7 0.0 4.641002233714375E-5 0.0 0.0 4.641002233714375E-5 8 0.0 4.641002233714375E-5 0.0 0.0 4.641002233714375E-5 9 0.0 4.641002233714375E-5 0.0 4.641002233714375E-5 4.641002233714375E-5 10-11 0.0 4.641002233714375E-5 0.0 4.641002233714375E-5 4.641002233714375E-5 12-13 0.0 4.641002233714375E-5 0.0 6.961503350571562E-5 2.088451005171469E-4 14-15 0.0 4.641002233714375E-5 0.0 1.3923006701143124E-4 2.784601340228625E-4 16-17 0.0 4.641002233714375E-5 0.0 1.85640089348575E-4 3.2487015636000627E-4 18-19 0.0 4.641002233714375E-5 0.0 3.4807516752857813E-4 4.641002233714375E-4 20-21 0.0 4.641002233714375E-5 0.0 4.873052345400094E-4 5.337152568771532E-4 22-23 2.3205011168571875E-5 4.641002233714375E-5 0.0 7.193553462257281E-4 5.56920268045725E-4 24-25 4.641002233714375E-5 4.641002233714375E-5 0.0 0.0012298655919343094 5.56920268045725E-4 26-27 4.641002233714375E-5 4.641002233714375E-5 0.0 0.002018835971665753 5.801252792142969E-4 28-29 4.641002233714375E-5 4.641002233714375E-5 0.0 0.005847662814480113 6.497403127200125E-4 30-31 4.641002233714375E-5 4.641002233714375E-5 0.0 0.018146318733823205 6.497403127200125E-4 32-33 4.641002233714375E-5 4.641002233714375E-5 0.0 0.0509117945038467 6.497403127200125E-4 34-35 4.641002233714375E-5 4.641002233714375E-5 0.0 0.10374960493468485 6.497403127200125E-4 36-37 4.641002233714375E-5 4.641002233714375E-5 0.0 0.1900026314482665 6.497403127200125E-4 38-39 4.641002233714375E-5 4.641002233714375E-5 0.0 0.3203451791821348 6.729453238885845E-4 40-41 4.641002233714375E-5 4.641002233714375E-5 0.0 0.45507347402686305 6.961503350571563E-4 42-43 4.641002233714375E-5 4.641002233714375E-5 0.0 0.5948140512840029 6.961503350571563E-4 44-45 4.641002233714375E-5 4.641002233714375E-5 0.0 0.7732837921814891 6.961503350571563E-4 46-47 4.641002233714375E-5 4.641002233714375E-5 0.0 0.9812935122965675 6.961503350571563E-4 48-49 4.641002233714375E-5 4.641002233714375E-5 0.0 1.1949188451144401 6.961503350571563E-4 50-51 4.641002233714375E-5 4.641002233714375E-5 0.0 1.4219334693765788 6.961503350571563E-4 52-53 4.641002233714375E-5 4.641002233714375E-5 0.0 1.64013018939466 6.961503350571563E-4 54-55 4.641002233714375E-5 4.641002233714375E-5 0.0 1.8776334787049933 6.961503350571563E-4 56-57 4.641002233714375E-5 4.641002233714375E-5 0.0 2.148319933986384 7.193553462257281E-4 58-59 1.3923006701143124E-4 4.641002233714375E-5 0.0 2.4323724757008724 7.425603573943E-4 60-61 1.3923006701143124E-4 4.641002233714375E-5 0.0 2.7129210607289065 7.425603573943E-4 62-63 1.3923006701143124E-4 4.641002233714375E-5 0.0 2.983932386166657 7.889703797314437E-4 64-65 1.3923006701143124E-4 9.28200446742875E-5 0.0 3.2652235315520857 7.889703797314437E-4 66-67 1.3923006701143124E-4 9.28200446742875E-5 0.0 3.5730844147255287 7.889703797314437E-4 68-69 1.3923006701143124E-4 9.28200446742875E-5 0.0 3.9169826802437644 7.889703797314437E-4 70-71 1.3923006701143124E-4 9.28200446742875E-5 0.0 4.30213945561972 7.889703797314437E-4 72-73 2.3205011168571875E-4 9.28200446742875E-5 0.0 4.70541934471833 8.353804020685876E-4 74-75 2.3205011168571875E-4 9.28200446742875E-5 0.0 5.108560003749929 9.049954355743032E-4 76-77 2.3205011168571875E-4 9.28200446742875E-5 0.0 5.536530024731901 9.28200446742875E-4 78-79 2.3205011168571875E-4 9.28200446742875E-5 0.0 6.014692484871492 9.28200446742875E-4 80-81 2.3205011168571875E-4 9.28200446742875E-5 0.0 6.524576195278523 9.28200446742875E-4 82-83 2.3205011168571875E-4 9.28200446742875E-5 0.0 7.040562823622887 9.28200446742875E-4 84-85 2.3205011168571875E-4 9.28200446742875E-5 0.0 7.533228415742837 9.28200446742875E-4 86-87 2.3205011168571875E-4 9.28200446742875E-5 0.0 8.03916727425121 9.514054579114468E-4 88-89 2.3205011168571875E-4 9.28200446742875E-5 0.0 8.585343622125885 9.746104690800187E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3630 0.0 49.145008 1 GTATCAA 6145 0.0 38.9913 1 TCAACGC 7740 0.0 30.701096 4 ATCAACG 7785 0.0 30.52363 3 TATCAAC 7790 0.0 30.448805 2 CAACGCA 7865 0.0 30.152851 5 AACGCAG 8135 0.0 29.210382 6 CGCGTAG 35 0.008399905 27.139 32-33 ACGCAGA 9445 0.0 25.058535 7 CGCAGAG 9535 0.0 24.822008 8 TGGTATC 1845 0.0 24.68383 2 GTGGTAT 1920 0.0 23.722925 1 GCAGAGT 10610 0.0 21.725908 9 GTACATG 6115 0.0 21.181873 1 TACATGG 6095 0.0 20.78142 2 GAGTACT 6810 0.0 20.26763 12-13 AGAGTAC 9555 0.0 19.731655 10-11 ACATGGG 6295 0.0 19.665358 3 CAGAGTA 10130 0.0 19.524668 10-11 GTACTTT 7115 0.0 19.198826 14-15 >>END_MODULE