FastQCFastQC Report
Fri 27 May 2016
SRR939739_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939739_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683908
Sequences flagged as poor quality0
Sequence length101
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152122.224275779783246No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA114651.6763950706820216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104851.5331009434017442No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86281.2615731940553407No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35610.5206840686174163No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33040.4831059148306498No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26980.39449750551243734No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23920.3497546453616568No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA20670.3022336337636056No Hit
GTACATGGGGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAAA18650.27269749732420145No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17410.25456640366832967No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA15710.22970925914011825No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14990.21918152733993462No Hit
GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA11870.17356135620580546No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT11240.16434959088064477No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT11120.16259496891394748No Hit
GAGTACATGGGGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAA10810.15806219549997952No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9980.14592606023032337No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT9720.1421243793024793No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA8820.12896471455224973No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA8430.12326219316048358No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8360.12223866367991017No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT8290.12121513419933674No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA8200.1198991677243138No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG8140.11902185674096516No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT8020.11726723477426788No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG7860.11492773881867152No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG7770.11361177234364857No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA7520.10995630991302924No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT7170.10483866251016219No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTATT800.065.2047961
GTATGGT1000.056.9060021
ATACATG1150.053.60711
TGTATCG555.0075698E-543.1137669
GCGCAGA1103.45608E-1143.1106071
ATGGATG459.690304E-442.161865
TGGGATA1650.040.2454156
AGATCAG951.9508661E-839.9428185
GATGGCC853.6830716E-739.0569
GATCAGT1003.0660885E-837.9456756
CCTCATC1302.0918378E-1036.4862253
ATCGAGT401.0325033E-535.84863790-91
ATGGTGG1651.8189894E-1234.4960673
TATGGTG1651.8189894E-1234.4884832
CATGGAT702.0697809E-433.8800664
GCGTCCG300.003831988231.86545690-91
AGGTCGC300.003834067131.86192788-89
CGTATCA1201.5262412E-731.6144471
CATGGGG27500.031.3914244
ATGGGAT16700.030.9586725
Produced by FastQC (version 0.11.5)