FastQCFastQC Report
Fri 27 May 2016
SRR939739_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939739_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683908
Sequences flagged as poor quality0
Sequence length101
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167402.447697643542699No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137032.0036320674710635No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA128001.8715967644770934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127861.8695497055159467No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46520.6802084490896436No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38300.5600168443708803No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34980.5114723032922557No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29640.43339162577422696No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24420.3570655702228955No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22190.3244588453417711No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA18200.26611766494908673No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15520.22693110769284758No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT13220.1933008533311498TruSeq Adapter, Index 3 (95% over 21bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12830.18759833193938366No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT11480.16785883481403932No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT11170.16332606140007136No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10740.1570386660194061No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC9860.1441714382636261No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA9600.140369757335782No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG9120.1333512694689929No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9000.13159664750229563No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT8730.12764874807722676No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT8630.12618656310497903No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG8630.12618656310497903No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8500.12428572264105697No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8010.1171210162770431No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC7830.11448908332699721No Hit
GTACATGGGGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAAA7730.11302689835474948No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT7620.11141849488527697RNA PCR Primer, Index 3 (95% over 23bp)
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA7600.11112605789082741No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC7570.1106874023991531No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7390.1080554694491072No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC7360.10761681395743286No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA7360.10761681395743286No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT7220.10556975499628606No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG7160.10469244401293742No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA7090.103668914532364No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT7090.103668914532364No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT7010.10249916655456581No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG6920.10118320007954287No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA6890.10074454458786854No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTTAC150.00954277748.45729472-73
CGTATCA608.670947E-539.2803962
GGACTCG250.001417427339.0271268-69
ATGGGCG1353.3469405E-1034.9133455
GGTATCA78850.034.0222431
AGGGTCT1001.3792651E-632.9931145
CCGGATG300.003464231532.5287664-65
TAGGCGC300.00346744632.52260268-69
AGCCGGT300.003519198932.4243862-63
CGTCCGC300.003519198932.4243862-63
CTTACGT300.003519198932.4243862-63
GTCGCAC300.00358342732.30486372-73
CAACGTG504.21605E-529.1819462-63
AAGGCGC350.007361203427.88179464-65
CGCTAGA350.007368010527.87651368-69
GAGCGTC350.00749138427.78183670-71
GCGTATC856.690139E-427.7334251
GCGCATT350.007613588627.6898872-73
AGCGACG350.007613588627.6898872-73
GTATCAA112450.027.4621331
Produced by FastQC (version 0.11.5)