FastQCFastQC Report
Fri 27 May 2016
SRR939738_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939738_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759511
Sequences flagged as poor quality0
Sequence length101
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167482.205103020232755No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130921.7237406699837132No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113581.4954358791380244No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102801.353502450919078No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39800.5240213769122501No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37100.4884721880262432No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29980.3947276602972175No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28210.3714231920275019No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23090.30401139680662953No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20380.26833054425808184No Hit
GTACATGGGGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAAA20380.26833054425808184No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA18080.2380479018737056No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17270.22738314520790354No Hit
GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA12850.16918780636488476No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT12370.162867950562928No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT11890.1565480947609712No Hit
GAGTACATGGGGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAA11880.15641643109843043No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11260.14825328402090293No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT10950.14417171048213917No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA10430.13732520003001933No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9400.12376384278832038No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA9240.12165722418766813No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA9130.1202089238997197No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT9070.1194189419244751No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG8940.11770731431144513No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT8730.11494237739808903No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG8450.11125579484694759No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG8400.11059747653424375No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA8360.11007082188408067No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT8120.1069108939831023No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG8120.1069108939831023No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC7900.10401429340720543No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT7880.10375096608212389No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG7700.1013810201563901No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTATT708.531242E-847.0504571
CAGTGCG256.929989E-445.1357192-93
GGGGCTA704.592888E-640.336947
TACCTGG1550.039.4668732
ACCTGGG1700.035.9868743
TGTCGCG352.161116E-433.6351884-85
CATGGGT9500.032.1987884
TGAGCGG300.00411030631.41252150-51
GGTTAGC600.00413430631.3731777
GTATGGT1351.312219E-831.366971
TGGGAGT2800.030.2527056
GTATCAA116200.030.0037771
ATGGATG804.743943E-429.412355
TGGGTAT2900.029.2095056
CATGGGG31050.029.0997564
GTACATG97850.028.754391
TGTATCG1154.152642E-628.6450714
ATCGTGA1154.152642E-628.6450717
ATGGTGG1652.921297E-928.5210653
ATGGGGG18250.028.3647865
Produced by FastQC (version 0.11.5)