FastQCFastQC Report
Fri 27 May 2016
SRR939738_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939738_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759511
Sequences flagged as poor quality0
Sequence length101
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187792.4725119188530513No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154162.0297270217284544No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA144271.8995116594756363No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139581.8377614017440167No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52230.6876793094504228No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43310.5702353224640591No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40150.528629605101177No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31650.4167154919415255No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27510.3622067356496483No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23600.31072624359620865No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA19330.25450585969130135No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17070.22474987195708818No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14790.19473055689779345No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT13150.17313771624110777TruSeq Adapter, Index 3 (95% over 21bp)
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT13070.17208440694078164No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12080.15904970434924576No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT11470.151018220934259No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC11190.14733163838311755No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA10430.13732520003001933No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG10290.1354819087544486No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10100.13298029916617404No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT9560.12587046138897265No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9550.12573879772643187No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG9320.12271053348799424No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC9290.12231554250037195No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT9290.12231554250037195No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT8920.1174439869863636No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8840.11639067768603746No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT8830.11625901402349668RNA PCR Primer, Index 3 (95% over 23bp)
GTACATGGGGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAAA8470.11151912217202911No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA8450.11125579484694759No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC8360.11007082188408067No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8250.10862252159613225No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG8210.10809586694596918No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA7960.10480427538245003No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT7960.10480427538245003No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT7950.10467261171990927No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA7950.10467261171990927No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG7940.1045409480573685No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC7740.10190767480655316No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACG205.202784E-447.83950464-65
GTATGGT1006.4028427E-1042.7180751
GGTTTGC550.002595237234.5014768
AGGGTCT1255.7170837E-934.1564645
GCCGACG300.003815945231.89300564-65
TACCTGG1351.2070814E-831.6367682
GGATGCT600.003974822331.6263548
TAGACCC902.4329833E-531.6263545
GACTTAC600.003974822331.6263547
GGTATGG902.4329833E-531.6263548
GGTATCA99300.028.7750741
TCTAGAC1004.9941013E-528.463723
ATGAGAG1703.6870915E-927.9056055
GTATCAA139600.027.5063081
CTATGCG350.00810581927.3368666-67
AGCGCCT350.00810581927.3368666-67
ATGGGCG1755.0240487E-927.1083055
TGGGACC700.00844209727.1083058
GGTACTC1955.4205884E-1026.7607633
TAGGGTC1658.506686E-825.8761084
Produced by FastQC (version 0.11.5)