FastQCFastQC Report
Fri 27 May 2016
SRR939737_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939737_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1651758
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100110.606081520416429No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72220.43723111981295076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52490.3177826291744917No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23780.14396782095197966No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA23730.14366511317033126No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA23300.141061826248155No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23120.13997207823422075No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA21660.13113301101008742No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG21350.12925622276386733No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC18250.11048834030166646No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA17920.10849046894278701No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG17670.106976930034545No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA17510.10600826513327012No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16580.10037790039460986No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49300.042.8186451
GTATCAA87800.040.2514571
TATCAAC110450.032.2987062
ATCAACG113850.031.2110353
TCAACGC115100.030.8720784
CAACGCA117150.030.2499395
AACGCAG121550.029.2329796
GTACATG87300.026.4083881
TACATGG89300.026.4020672
TAAGGTA4150.026.2929384
ACGCAGA133400.026.2805717
AGGTAAG8100.025.7691842
CGCAGAG136250.025.7641078
CGTATCA1859.100404E-925.6417771
TAAGGTG9050.025.1616345
AAGGTAA8150.025.0282631
ACATGGG92800.024.6410083
GCAGAGT140500.023.971149
GGTAAGG9950.023.3632013
GTGTAAG5350.023.0536371
Produced by FastQC (version 0.11.5)