##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939737_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1651758 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.071103636246956 31.0 30.0 33.0 16.0 34.0 2 29.9090405495236 31.0 30.0 33.0 25.0 34.0 3 30.18467838509031 31.0 30.0 34.0 26.0 34.0 4 33.99667869021975 35.0 35.0 37.0 30.0 37.0 5 33.96492706558709 35.0 35.0 37.0 28.0 37.0 6 34.0229361686155 36.0 35.0 37.0 29.0 37.0 7 33.93943664871004 36.0 35.0 37.0 29.0 37.0 8 33.80748753752063 35.0 35.0 37.0 28.0 37.0 9 35.092862877007406 37.0 35.0 39.0 28.0 39.0 10-11 34.92551420970869 37.0 34.0 39.0 27.0 39.0 12-13 34.78257680604544 37.0 34.0 39.0 27.0 39.0 14-15 35.43534010430099 38.0 34.0 40.0 27.0 41.0 16-17 35.26974653672027 38.0 34.0 40.0 27.0 41.0 18-19 35.146531453154765 38.0 33.5 40.0 27.0 41.0 20-21 35.326408590120344 38.0 34.0 40.0 27.0 41.0 22-23 35.2280930378421 38.0 34.0 40.0 26.5 41.0 24-25 34.98339345109877 38.0 34.0 40.0 25.0 41.0 26-27 34.835429282013465 38.0 33.5 40.0 25.0 41.0 28-29 34.85967102929121 38.0 34.0 40.0 25.0 41.0 30-31 34.58626475549082 38.0 33.5 40.0 24.0 41.0 32-33 34.16604853737654 38.0 33.0 40.0 23.5 41.0 34-35 33.9470960637091 38.0 33.0 40.0 22.5 41.0 36-37 33.68171154612237 37.5 33.0 40.0 21.0 41.0 38-39 33.39386066239727 37.0 32.0 40.0 19.5 41.0 40-41 33.08193603421324 37.0 31.5 40.0 18.0 41.0 42-43 32.61768552051814 37.0 31.0 40.0 15.0 41.0 44-45 32.18343334798439 36.5 30.5 39.5 11.5 41.0 46-47 31.692867841415026 36.0 30.0 39.0 8.5 41.0 48-49 31.228620052089955 36.0 29.5 39.0 7.5 40.0 50-51 30.520434591507957 35.0 28.5 38.5 4.0 40.0 52-53 30.622922667848435 35.0 28.5 38.5 2.0 40.0 54-55 30.673496056928435 35.0 29.0 39.0 2.0 40.0 56-57 30.319271951460202 35.0 28.0 39.0 2.0 40.0 58-59 29.879499599820313 35.0 27.5 38.0 2.0 40.0 60-61 29.429684009400894 34.0 27.0 38.0 2.0 40.0 62-63 28.95274489362243 34.0 26.0 38.0 2.0 40.0 64-65 28.372111410993618 34.0 26.0 37.0 2.0 39.5 66-67 27.751108213188616 33.0 25.0 36.5 2.0 39.0 68-69 27.171894732763516 33.0 23.5 36.0 2.0 39.0 70-71 26.55982323076383 32.0 22.0 35.0 2.0 38.0 72-73 25.91654861063182 32.0 20.0 35.0 2.0 37.5 74-75 25.568517906376115 31.5 19.5 35.0 2.0 37.0 76-77 25.01511117245989 31.0 18.0 35.0 2.0 36.5 78-79 24.365444877518378 31.0 11.5 34.0 2.0 36.0 80-81 23.649693841349638 30.5 7.0 34.0 2.0 35.5 82-83 22.91543646224205 29.5 2.0 34.0 2.0 35.0 84-85 22.138388008412853 29.0 2.0 34.0 2.0 35.0 86-87 21.234483501820485 29.0 2.0 34.0 2.0 35.0 88-89 20.238132644128257 27.0 2.0 33.0 2.0 35.0 90-91 18.85345916290401 25.0 2.0 32.5 2.0 34.5 92-93 17.35299057125802 19.5 2.0 32.0 2.0 34.0 94-95 16.8188233385278 18.5 2.0 31.0 2.0 34.0 96-97 16.479488823423285 17.5 2.0 31.0 2.0 34.0 98-99 15.602834979458251 2.0 2.0 31.0 2.0 34.0 100-101 14.006471892371643 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.4916115339479177 1101 2 0.8104482424335551 1101 3 0.6736084056511338 1101 4 0.6411966288700128 1101 5 0.6603414446208475 1101 6 0.7596883368771543 1101 7 0.6488749238829215 1101 8 0.8423696397568179 1101 9 0.9417364298105255 1101 10-11 0.8469751108863335 1101 12-13 0.6727132479097264 1101 14-15 1.0317307025231415 1101 16-17 0.7965300342956425 1101 18-19 0.928062484591841 1101 20-21 0.7648493688825297 1101 22-23 0.8193675522835804 1101 24-25 0.9907882462880337 1101 26-27 0.7615722159971696 1101 28-29 0.8822824099675728 1101 30-31 0.7600509936975683 1101 32-33 1.1194833153976091 1101 34-35 0.9529538023172321 1101 36-37 0.8776224939871469 1101 38-39 0.7335285786604331 1101 40-41 0.9605758761694574 1101 42-43 0.7608949196644588 1101 44-45 0.8235892936768678 1101 46-47 0.6276711769510079 1101 48-49 0.6295706039237992 1101 50-51 0.45968681266099054 1101 52-53 0.6565447103812687 1101 54-55 0.668071524080208 1101 56-57 0.41520925290359045 1101 58-59 0.3205268859558963 1101 60-61 0.40915262360833893 1101 62-63 0.06002752808255707 1101 64-65 0.13335225312584242 1101 66-67 0.38775578545982725 1101 68-69 0.18685743911115793 1101 70-71 0.0018681355662764076 1101 72-73 0.18674414361297664 1101 74-75 -0.03773615941297592 1101 76-77 -0.1878112369348912 1101 78-79 -0.7769542082595784 1101 80-81 -1.331420058916624 1101 82-83 -1.2915682619040716 1101 84-85 -1.1730042473637532 1101 86-87 -1.2338954826700288 1101 88-89 -5.16698361359164 1101 90-91 -7.961923206358421 1101 92-93 -10.091427320290958 1101 94-95 -8.2428846804724 1101 96-97 -5.0551923463290525 1101 98-99 -3.6244149473704557 1101 100-101 -2.992879369977528 1102 1 1.9119474277636748 1102 2 1.249249920706145 1102 3 1.0683534342471752 1102 4 1.1043408374735506 1102 5 1.1023078313095311 1102 6 1.1117546701227425 1102 7 1.1492717587756758 1102 8 1.2065962703693387 1102 9 1.3698534781565783 1102 10-11 1.2958920906337994 1102 12-13 1.3256940961393298 1102 14-15 1.4967344515160974 1102 16-17 1.3835296035602767 1102 18-19 1.4739417829717993 1102 20-21 1.58244889666895 1102 22-23 1.5483133673704899 1102 24-25 1.7752450810212608 1102 26-27 1.738317491867214 1102 28-29 1.5459807915193409 1102 30-31 1.7097068042378254 1102 32-33 1.7744613000347087 1102 34-35 1.8555408068718577 1102 36-37 1.797930469770236 1102 38-39 1.7997441696851766 1102 40-41 1.8058611186974005 1102 42-43 1.7910234622633823 1102 44-45 1.7566807371986854 1102 46-47 1.522771163390825 1102 48-49 1.5819753436080894 1102 50-51 1.709432559149299 1102 52-53 1.590263312565252 1102 54-55 1.603731426606398 1102 56-57 1.6323906084437247 1102 58-59 1.516779687729887 1102 60-61 1.296263067345528 1102 62-63 1.2526659417001724 1102 64-65 1.3323499957534892 1102 66-67 1.4775830526034888 1102 68-69 1.291997747166711 1102 70-71 1.2159515865541728 1102 72-73 1.2768097111068144 1102 74-75 1.0462333062358802 1102 76-77 1.0727706945143716 1102 78-79 0.8545516904218893 1102 80-81 1.1059558055453174 1102 82-83 0.9511471257178705 1102 84-85 0.9490633621347584 1102 86-87 1.0644984327941707 1102 88-89 0.5798307394677771 1102 90-91 -5.223019627266634 1102 92-93 -10.142356791363888 1102 94-95 -8.02894879622854 1102 96-97 -4.624951533355942 1102 98-99 -3.180482333524706 1102 100-101 -2.280623553056012 1103 1 2.141149378296717 1103 2 1.4558701967589336 1103 3 1.1311525800707933 1103 4 1.0983476937935563 1103 5 1.1151859311761712 1103 6 0.8810247747675319 1103 7 0.7805666050811979 1103 8 0.9052822787311072 1103 9 1.0564202454589733 1103 10-11 0.9658058957277618 1103 12-13 0.859390699888209 1103 14-15 0.9710876829046313 1103 16-17 0.8744959110474184 1103 18-19 0.9183548639794665 1103 20-21 0.7743276756221746 1103 22-23 0.9360513544923919 1103 24-25 1.0578923732657586 1103 26-27 1.1519047463119634 1103 28-29 0.9960464501924235 1103 30-31 0.7795586324534582 1103 32-33 0.9081155631439444 1103 34-35 0.7719016800299414 1103 36-37 0.8455790301884889 1103 38-39 0.5895648253719159 1103 40-41 0.5868362624919357 1103 42-43 0.6497499294585936 1103 44-45 0.5096149230720428 1103 46-47 0.45096593851497957 1103 48-49 0.4038879541642011 1103 50-51 0.3589205851345909 1103 52-53 0.35347072851806516 1103 54-55 0.17935242265459905 1103 56-57 0.24352456031857628 1103 58-59 0.4140995456944303 1103 60-61 0.2523607330876523 1103 62-63 0.46966274262962315 1103 64-65 0.44400451249747164 1103 66-67 0.29568500559106425 1103 68-69 0.24706451037821608 1103 70-71 0.28363524674906415 1103 72-73 0.2851407944544704 1103 74-75 0.2026175383362201 1103 76-77 0.12017073378685339 1103 78-79 -0.16365887551087255 1103 80-81 -0.36743913648687254 1103 82-83 -0.7498297771563855 1103 84-85 -0.9538599828984999 1103 86-87 -1.1833313537435544 1103 88-89 -1.6778386435452397 1103 90-91 -6.663884889942235 1103 92-93 -9.94281681116376 1103 94-95 -8.659114558810051 1103 96-97 -5.94005998266581 1103 98-99 -4.383469905421977 1103 100-101 -3.6631814595056973 1104 1 1.9679719551359263 1104 2 1.2352533224110473 1104 3 1.0585613837075591 1104 4 0.7171390772262569 1104 5 0.5841417822142887 1104 6 0.736346161810836 1104 7 0.6673139561150592 1104 8 0.767230985307819 1104 9 0.8754234324630445 1104 10-11 0.9097835667982395 1104 12-13 0.6833715551197628 1104 14-15 0.9617524049586379 1104 16-17 0.9921815092574633 1104 18-19 0.9119062270562708 1104 20-21 0.9363227231145075 1104 22-23 0.8227354487549263 1104 24-25 0.8570047883666447 1104 26-27 0.7279012892227996 1104 28-29 0.7889217476413961 1104 30-31 0.8350690790604816 1104 32-33 0.5835698036220265 1104 34-35 0.5505504585345733 1104 36-37 0.6411945541109247 1104 38-39 0.7842640500855538 1104 40-41 0.5034743569989715 1104 42-43 0.6966155209022915 1104 44-45 0.5624607670310979 1104 46-47 0.40715031849911654 1104 48-49 0.3589573091725775 1104 50-51 0.39394450898765143 1104 52-53 0.3196907597342111 1104 54-55 0.19364020826958495 1104 56-57 0.17809005026714786 1104 58-59 0.12854071121110167 1104 60-61 0.18311773895276318 1104 62-63 0.16100011871031938 1104 64-65 0.09165946315719253 1104 66-67 -0.05678243494046242 1104 68-69 -0.167004790613138 1104 70-71 -0.25411611007314505 1104 72-73 -0.21666394255372978 1104 74-75 -0.31227196843154204 1104 76-77 -0.33233338145703684 1104 78-79 -0.6876928948603371 1104 80-81 -0.6200544130682211 1104 82-83 -0.6842778511653833 1104 84-85 -0.6089097212002592 1104 86-87 -0.5395461538379394 1104 88-89 -0.792833625648548 1104 90-91 -3.3486766827670706 1104 92-93 -7.584940703459512 1104 94-95 -5.631527072563069 1104 96-97 -3.1627158573008387 1104 98-99 -2.297624496442074 1104 100-101 -1.728961678473631 1105 1 2.0344887580961846 1105 2 1.1399792766495764 1105 3 0.9325661809260524 1105 4 0.7753333662679793 1105 5 0.8056923637543107 1105 6 0.6615654136546567 1105 7 0.6153797494419493 1105 8 0.5945907039071159 1105 9 0.7821959065176074 1105 10-11 0.7786740957618292 1105 12-13 0.8776633052242886 1105 14-15 1.0053470010629724 1105 16-17 0.9637758480656728 1105 18-19 0.7499992565319147 1105 20-21 0.7863238233939782 1105 22-23 0.7431935374171417 1105 24-25 0.9004092900331457 1105 26-27 0.7445292204096248 1105 28-29 0.7035696836017422 1105 30-31 0.9183582037289142 1105 32-33 1.1915219465432543 1105 34-35 1.0663922823578247 1105 36-37 1.1025452910442581 1105 38-39 0.9237298897934565 1105 40-41 0.9052798309739742 1105 42-43 1.0057217723516985 1105 44-45 0.9762890794224433 1105 46-47 0.8781698036021801 1105 48-49 0.8378578422156657 1105 50-51 0.9510339481667422 1105 52-53 0.8011547746709304 1105 54-55 0.7883837500522759 1105 56-57 0.8230721834212744 1105 58-59 0.9332021721452541 1105 60-61 0.9118639789592642 1105 62-63 0.8206984051519974 1105 64-65 0.7306049553121987 1105 66-67 0.7176255723857032 1105 68-69 0.7518396112495509 1105 70-71 0.6353206991840992 1105 72-73 0.5344497941815547 1105 74-75 0.325697152648285 1105 76-77 0.1204934057776903 1105 78-79 0.06575817401884976 1105 80-81 0.2338861955386733 1105 82-83 -0.17870992922090423 1105 84-85 0.22584506036040963 1105 86-87 0.37459040607904726 1105 88-89 0.06922738400174921 1105 90-91 -3.163131646635584 1105 92-93 -7.369338909834701 1105 94-95 -5.384359615105797 1105 96-97 -2.558869280270274 1105 98-99 -1.5600816067019263 1105 100-101 -1.035927970988853 1106 1 1.6426950107848164 1106 2 0.9915507355709892 1106 3 0.7050461616877612 1106 4 0.6460907155920594 1106 5 0.7630593987622305 1106 6 0.8578467688005915 1106 7 0.7712184451377482 1106 8 -0.46877135822980875 1106 9 -0.4974742640150609 1106 10-11 -0.7768616455806381 1106 12-13 -0.2789608051164123 1106 14-15 0.6013806469508509 1106 16-17 0.6939364609937542 1106 18-19 0.9004958327577555 1106 20-21 0.876143614705633 1106 22-23 0.793237266347333 1106 24-25 0.8183554967464843 1106 26-27 0.9579526005401604 1106 28-29 0.7712439097743555 1106 30-31 0.6825211572616894 1106 32-33 0.7454395346723786 1106 34-35 0.7210919541775951 1106 36-37 0.9497231400358572 1106 38-39 1.0808753757207867 1106 40-41 1.0409711278810292 1106 42-43 1.0376093632065277 1106 44-45 0.8944434707778868 1106 46-47 0.8032816241433629 1106 48-49 0.7276109738204433 1106 50-51 0.744580792960928 1106 52-53 1.1031171535734785 1106 54-55 1.1245907761722371 1106 56-57 0.9334470092062723 1106 58-59 0.9511570312154234 1106 60-61 0.7018832619347997 1106 62-63 0.9810054541418012 1106 64-65 0.8391692855852497 1106 66-67 0.8688811593506252 1106 68-69 0.8139038198615296 1106 70-71 0.7059135724855672 1106 72-73 0.6063096487488409 1106 74-75 0.7245171824075207 1106 76-77 0.7405340921655004 1106 78-79 0.5539101321528186 1106 80-81 0.375652421855893 1106 82-83 0.033453172173750545 1106 84-85 -0.1805080037316209 1106 86-87 -0.2773329965963569 1106 88-89 -0.8346333544870639 1106 90-91 -4.102606582626423 1106 92-93 -8.530858507422927 1106 94-95 -6.101632278492151 1106 96-97 -3.2653015154583933 1106 98-99 -2.1848530605862866 1106 100-101 -1.4607714872254274 1107 1 1.7608278348883566 1107 2 1.0318126251772455 1107 3 0.8437611647694077 1107 4 0.9130600910783002 1107 5 0.5504842520050346 1107 6 0.6397978059521421 1107 7 0.444995737934768 1107 8 0.47898206947269983 1107 9 0.4706253186994118 1107 10-11 0.36312279462022445 1107 12-13 0.38521063557953283 1107 14-15 0.4483550743199274 1107 16-17 0.35054705176094103 1107 18-19 0.5410216327234068 1107 20-21 0.3724913054899872 1107 22-23 0.27667759855914653 1107 24-25 0.3548137227273074 1107 26-27 0.2404506555652688 1107 28-29 0.2163521716235408 1107 30-31 0.391374076744782 1107 32-33 0.4168610334649756 1107 34-35 0.64262489750557 1107 36-37 0.15894706979447903 1107 38-39 0.25880996394182176 1107 40-41 0.24867161185139963 1107 42-43 0.38266831867922946 1107 44-45 0.09682310966929464 1107 46-47 -0.05217855186423748 1107 48-49 0.3693526609472393 1107 50-51 0.28870009822600196 1107 52-53 0.21116480293239803 1107 54-55 0.20702659200079765 1107 56-57 0.03183262952696708 1107 58-59 0.23727465925539448 1107 60-61 0.1663024469650587 1107 62-63 0.08240300700975922 1107 64-65 0.10718217799025354 1107 66-67 0.08667005127064442 1107 68-69 0.34612897217579786 1107 70-71 0.19773518039883697 1107 72-73 -0.020608197532467898 1107 74-75 -0.06059330227011728 1107 76-77 -0.06320955509182724 1107 78-79 -0.16327455457992457 1107 80-81 -0.1461585771034173 1107 82-83 -0.4113343961529665 1107 84-85 -0.5409875284504828 1107 86-87 -0.21388155024230215 1107 88-89 -0.2859665463676748 1107 90-91 -3.463976249101119 1107 92-93 -4.629018005467849 1107 94-95 -3.0911346680327334 1107 96-97 -1.8525899678646063 1107 98-99 -1.1493082150223444 1107 100-101 -0.7088538935382598 1108 1 1.5899049250077937 1108 2 0.8541703109225551 1108 3 -0.6444338613409251 1108 4 -0.41414222524453237 1108 5 0.31566702435393523 1108 6 0.30671368413837286 1108 7 0.38155951436825575 1108 8 0.20534058247616116 1108 9 0.6252326295933699 1108 10-11 0.5892972426839265 1108 12-13 0.43289793199647164 1108 14-15 0.23572646152845067 1108 16-17 0.4963001152012936 1108 18-19 0.6167586680369865 1108 20-21 0.39841602499776485 1108 22-23 0.2766682919048904 1108 24-25 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0.7377025977641338 2311 80-81 1.0689080509128495 2311 82-83 1.2249945187370983 2311 84-85 1.459746653434781 2311 86-87 1.759593208412312 2311 88-89 2.1298730896158453 2311 90-91 2.3919832031173236 2311 92-93 3.3391075380533763 2311 94-95 3.11142275855347 2311 96-97 2.282689940077173 2311 98-99 1.9609713854730852 2311 100-101 1.8753892174674416 2312 1 1.0927623586978825 2312 2 0.2989108394629625 2312 3 -0.07938119634170349 2312 4 -0.7715480438423299 2312 5 -0.31786892259813726 2312 6 -0.38118681706373536 2312 7 1.569482522327803E-4 2312 8 0.0668416924885733 2312 9 0.12309803695337962 2312 10-11 -0.1228320664908864 2312 12-13 -0.11022835251570484 2312 14-15 0.2125167806691337 2312 16-17 -0.46542021014383295 2312 18-19 -0.7705396271972305 2312 20-21 -0.02986608538302704 2312 22-23 0.09764486046391596 2312 24-25 0.42434001240984287 2312 26-27 0.22504390953351816 2312 28-29 0.24718674503624527 2312 30-31 0.06205240015748359 2312 32-33 0.3763042378300625 2312 34-35 0.34226651456906154 2312 36-37 0.3492236074928883 2312 38-39 0.2851976127513396 2312 40-41 0.1581371376450491 2312 42-43 0.13504183058399377 2312 44-45 0.05938387355818264 2312 46-47 0.07465428543735086 2312 48-49 0.3701525121377145 2312 50-51 0.19328715179742773 2312 52-53 0.2104951600752507 2312 54-55 0.1096419193817475 2312 56-57 0.042762689050778846 2312 58-59 0.07732798266809127 2312 60-61 0.08395745688569534 2312 62-63 0.20874725304150488 2312 64-65 0.16221442005374342 2312 66-67 0.3618176207150867 2312 68-69 0.2366535257472222 2312 70-71 0.12797079547819834 2312 72-73 -0.1343333959451627 2312 74-75 0.17403839415845468 2312 76-77 0.6043636095907097 2312 78-79 0.5963124989915052 2312 80-81 0.6860090816267395 2312 82-83 0.49209814352957437 2312 84-85 0.7807907453590417 2312 86-87 1.2532699874561075 2312 88-89 1.4598977889497817 2312 90-91 2.312886350105231 2312 92-93 2.9050556552464357 2312 94-95 2.5005518399037747 2312 96-97 1.7248033853099969 2312 98-99 1.5874365766443201 2312 100-101 1.4315578128109472 2313 1 -7.0069023856045085 2313 2 -2.7146687533663894 2313 3 -1.609495582806403 2313 4 -0.8057293598512274 2313 5 -0.4937131416087013 2313 6 0.02052290014027136 2313 7 -0.0729413876288163 2313 8 0.09574446833626382 2313 9 0.1212806210278714 2313 10-11 -0.06502000075992953 2313 12-13 -0.24144491379975364 2313 14-15 -0.0497426342836107 2313 16-17 -0.3801514844628926 2313 18-19 -0.5201802695679945 2313 20-21 -0.30695035963261574 2313 22-23 -0.7752141931770069 2313 24-25 -0.10963936849621092 2313 26-27 -0.25618698262879036 2313 28-29 -0.20480155575586423 2313 30-31 0.17099855948211484 2313 32-33 -0.19626971818717465 2313 34-35 -0.12514485112021134 2313 36-37 0.1571040705973914 2313 38-39 -0.0772248702253151 2313 40-41 0.05365092134432814 2313 42-43 -0.07847305615863576 2313 44-45 -0.10131412706716247 2313 46-47 -1.2975729067527553 2313 48-49 -0.665740909554895 2313 50-51 -0.5128896738696085 2313 52-53 -1.0797746078513732 2313 54-55 -0.7908203600095796 2313 56-57 -0.4417230495540032 2313 58-59 -0.18630263677326298 2313 60-61 -0.0103277797040775 2313 62-63 -0.051809827280898446 2313 64-65 0.04865089517881316 2313 66-67 0.01951916497578665 2313 68-69 -0.10910171153737735 2313 70-71 -0.07213820605042542 2313 72-73 0.07848883168129461 2313 74-75 0.2037869089583424 2313 76-77 0.37611723139448827 2313 78-79 0.28111677393591705 2313 80-81 0.5051229990808785 2313 82-83 0.7489595943355809 2313 84-85 1.0165184934135176 2313 86-87 1.522196517820202 2313 88-89 1.632883432015376 2313 90-91 2.609754836069655 2313 92-93 3.6229664303298144 2313 94-95 2.8146673207264534 2313 96-97 1.8045590655490216 2313 98-99 1.4705324371918476 2313 100-101 1.119218492355138 2314 1 0.8233931190665444 2314 2 0.19547621964729345 2314 3 -1.005990499337095 2314 4 -0.20937206457966084 2314 5 0.26493637694103 2314 6 -3.6233563159116606 2314 7 -2.5459079999044505 2314 8 -2.256873025253366 2314 9 -3.1285400678853286 2314 10-11 -2.3022855515139327 2314 12-13 -1.0218246946816052 2314 14-15 -1.0450077008976777 2314 16-17 -1.9174291387152564 2314 18-19 -2.8005925505843265 2314 20-21 -2.6616075168346427 2314 22-23 -3.8311065796282584 2314 24-25 -2.6727041984196447 2314 26-27 -1.6002009061346811 2314 28-29 -1.753956328120438 2314 30-31 -1.7596371879761534 2314 32-33 -1.9938084493819233 2314 34-35 -1.671279352366419 2314 36-37 -1.7017646557559587 2314 38-39 -2.9380256735389807 2314 40-41 -2.3489103133918157 2314 42-43 -1.4891481175726895 2314 44-45 -2.0738156512113974 2314 46-47 -1.62978479866403 2314 48-49 -2.434237968853072 2314 50-51 -3.066910500852341 2314 52-53 -2.0077868099708276 2314 54-55 -2.422643146862491 2314 56-57 -1.3251988604883955 2314 58-59 -0.9340937483226917 2314 60-61 -1.2985580241050876 2314 62-63 -0.9203210073271606 2314 64-65 -1.5218833622043206 2314 66-67 -0.8260514029024257 2314 68-69 -0.9320622720638347 2314 70-71 -1.0137522747982963 2314 72-73 -1.9502536148391734 2314 74-75 -1.9274250995051396 2314 76-77 -1.1741952181742654 2314 78-79 -1.7295533564232421 2314 80-81 -0.6438800312764847 2314 82-83 -0.5788984002492263 2314 84-85 -1.478191829525752 2314 86-87 -0.3587061790185473 2314 88-89 0.6502149692493226 2314 90-91 1.6999651231467041 2314 92-93 2.6437239300160194 2314 94-95 2.372527646355387 2314 96-97 1.758303529319214 2314 98-99 1.6484874839023878 2314 100-101 1.4740045294008066 2315 1 -2.733642002459799 2315 2 -1.8725939750168763 2315 3 -2.5316965447710658 2315 4 -3.94148112091813 2315 5 -3.666014272461368 2315 6 -3.0125209877378722 2315 7 -5.471282583976656 2315 8 -2.952687489435899 2315 9 -2.4893938975520413 2315 10-11 -3.6266418062994106 2315 12-13 -2.8862244066275906 2315 14-15 -1.8900852719647645 2315 16-17 -2.336983757968767 2315 18-19 -1.6529707396012796 2315 20-21 -1.120785751678362 2315 22-23 -0.4615358783078136 2315 24-25 -2.1207671276898665 2315 26-27 -3.787437615658394 2315 28-29 -3.0662816313314494 2315 30-31 -3.0164781149863416 2315 32-33 -3.5193125794844207 2315 34-35 -3.7884987629942515 2315 36-37 -3.0828078837825927 2315 38-39 -1.8635571645031384 2315 40-41 -1.5111974039039637 2315 42-43 -2.765902521639603 2315 44-45 -5.782224117209921 2315 46-47 -2.957803815352378 2315 48-49 -2.023520658856288 2315 50-51 -3.264464994864973 2315 52-53 -3.1787849014706744 2315 54-55 -1.8590182916722533 2315 56-57 -1.3355534700641947 2315 58-59 -2.5101628143957946 2315 60-61 -2.750233725667467 2315 62-63 -4.52954812880796 2315 64-65 -3.8004578506380398 2315 66-67 -3.343465933121056 2315 68-69 -1.5853825428687642 2315 70-71 -2.9485469651544634 2315 72-73 -2.1727189458878087 2315 74-75 -1.3274211501479982 2315 76-77 -2.867405953025102 2315 78-79 -1.3038975140787343 2315 80-81 -1.3372740020668985 2315 82-83 -0.83373956493244 2315 84-85 -2.385302949492278 2315 86-87 -1.7444532337231884 2315 88-89 -1.0080103319692135 2315 90-91 -0.14021417766764444 2315 92-93 0.7942097365245608 2315 94-95 0.5326511295887677 2315 96-97 0.17617362322140906 2315 98-99 -0.09757845826479361 2315 100-101 -0.309602543008344 2316 1 -7.299837988963308 2316 2 -6.183459182660933 2316 3 -3.3149858502607934 2316 4 -3.858295436383422 2316 5 -2.058505774684104 2316 6 -2.711321733308992 2316 7 -3.013043312493661 2316 8 -2.2529325252737564 2316 9 -7.258642836940421 2316 10-11 -6.907988316490886 2316 12-13 -6.130609002010019 2316 14-15 -5.014322268003429 2316 16-17 -4.69579513112992 2316 18-19 -4.286591796128967 2316 20-21 -2.96650433996583 2316 22-23 -3.3801028481328004 2316 24-25 -3.2204974164776417 2316 26-27 -4.12733835974587 2316 28-29 -4.229862184325327 2316 30-31 -6.690364931542895 2316 32-33 -4.895619652024553 2316 34-35 -5.4976883487368795 2316 36-37 -4.102931002968553 2316 38-39 -6.635193407318191 2316 40-41 -3.798486696899829 2316 42-43 -4.347639308796538 2316 44-45 -2.902454878812229 2316 46-47 -4.067190491743435 2316 48-49 -5.211283664379355 2316 50-51 -4.459138081293595 2316 52-53 -6.196041265335619 2316 54-55 -4.9867871458837385 2316 56-57 -4.284231424413193 2316 58-59 -2.9819991151511225 2316 60-61 -3.037993100162346 2316 62-63 -4.129896473254323 2316 64-65 -2.9284787294405703 2316 66-67 -3.9552824701509053 2316 68-69 -3.1689026333867822 2316 70-71 -2.4359465164687 2316 72-73 -2.0347857505595783 2316 74-75 -2.0778752238883236 2316 76-77 -2.6406066614269896 2316 78-79 -4.566068486311909 2316 80-81 -5.5210567369573305 2316 82-83 -3.0801600739166943 2316 84-85 -1.854308219558785 2316 86-87 -2.107327258909251 2316 88-89 -0.9974569242297378 2316 90-91 -1.1871087115256138 2316 92-93 -1.073859977654955 2316 94-95 -1.4990135836107648 2316 96-97 -3.0561157439567523 2316 98-99 -4.4521864282545405 2316 100-101 -3.9746032965443003 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12718.0 3 4222.0 4 4262.0 5 4607.0 6 5416.0 7 6417.0 8 7597.0 9 9309.0 10 11138.0 11 13321.0 12 16128.0 13 18107.0 14 20165.0 15 23310.0 16 26485.0 17 28420.0 18 25382.0 19 24163.0 20 24963.0 21 27585.0 22 30362.0 23 34417.0 24 38373.0 25 43180.0 26 49185.0 27 55982.0 28 64011.0 29 72365.0 30 82263.0 31 92143.0 32 103188.0 33 115236.0 34 131905.0 35 147831.0 36 146885.0 37 103440.0 38 26819.0 39 458.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.53090185813074 18.792454477282135 13.254374951881138 22.422268712705986 2 16.686911520291524 20.12399063645213 38.43715791349507 24.751939929761267 3 19.756766127898125 24.64653922438667 29.247227354505824 26.349467293209383 4 13.191134131991364 15.907505045416325 35.51172858479179 35.38963223780052 5 13.977211904315942 37.457727700623316 32.873437387291546 15.691623007769193 6 32.73676112275452 35.054947653168064 16.8496840517521 15.358607172325323 7 29.119905493207327 33.349605999337776 19.670439095150826 17.860049412304075 8 28.195819935496353 32.41887474540825 19.36855789654313 20.016747422552267 9 28.287054808351787 15.525728046126314 19.230071854057854 36.95714529146405 10-11 26.047470556400278 25.82295789743364 25.96551813004149 22.164053416124588 12-13 27.40302398726742 23.44619348971162 26.685058168176134 22.46572435484482 14-15 23.829749692356526 24.582434050304066 25.339756935128232 26.248059322211176 16-17 22.817697730714254 27.82952845360856 26.21215267619895 23.140621139478235 18-19 23.0116765707248 25.28777557552841 29.33001995365271 22.370527900094082 20-21 23.598229457375623 24.53765526208227 29.456556938627738 22.407558341914363 22-23 23.245178221101177 24.60086941857178 29.69966477762855 22.4542875826985 24-25 22.959259981742854 25.047182504445214 29.62348346283936 22.370074050972576 26-27 22.277335720191456 25.293606304946408 29.599538810464754 22.829519164397382 28-29 22.47959189246097 25.863032517656283 29.319986626189248 22.337388963693503 30-31 22.315940776803885 25.499108691141153 29.17501447087562 23.009936061179342 32-33 22.01938455788306 25.542134170167635 29.076745811581223 23.36173546036808 34-35 23.043755886773738 25.59560878909538 28.991092297028594 22.36954302710229 36-37 23.233267967352607 24.9910809413672 28.94431716415194 22.83133392712826 38-39 22.524050675147915 25.347007675892602 28.84036539736129 23.288576251598197 40-41 22.707186862302745 25.35312141952945 29.24412313679024 22.695568581377564 42-43 22.6709266183585 25.500561006882233 28.609955680607968 23.218556694151303 44-45 22.47898369530271 25.5997639040038 29.012722007277 22.908530393416488 46-47 23.156960738959963 26.046184997838633 28.07853724755614 22.718317015645262 48-49 22.670812354035068 26.278482887204614 27.66423938006477 23.386465378695554 50-51 22.830829625466507 26.504576593067707 27.759232542881474 22.905361238584312 52-53 23.247102909017414 26.121069360846466 27.658577531817475 22.973250198318645 54-55 22.451960382963474 26.321592086060868 27.95057368957551 23.27587384140015 56-57 22.55962443251666 26.624206797543305 27.47511325395949 23.34105551598055 58-59 22.60520008494888 25.884712235672463 27.61800309456631 23.892084584812352 60-61 22.010968222727534 26.238110706801592 28.24543316775751 23.505487902713362 62-63 22.369410323918625 26.153237715321744 28.2278964350845 23.249455525675135 64-65 21.986131797962077 26.514603526707496 27.815956833454496 23.683307841875926 66-67 21.989837824983955 26.435000782545714 27.82232087285998 23.752840519610352 68-69 22.72319457048709 25.882544562479676 28.053191076342216 23.341069790691016 70-71 22.41608845007621 26.117766605243002 27.46951187145013 23.996633073230658 72-73 22.328263325269504 26.38316735881293 27.34002531110172 23.948544004815844 74-75 22.338952636492813 26.900136935051883 27.136230805804978 23.624679622650326 76-77 22.515269673958663 26.259165825305885 27.353496252340864 23.872068248394587 78-79 22.58171313084722 26.61573662441547 27.197158437444052 23.605391807293262 80-81 22.89758471377943 26.61995593781763 26.897415967578876 23.585043380824057 82-83 22.6867682875938 26.587646152795585 26.92856438310432 23.797021176506295 84-85 22.37889547380443 26.783570831265013 26.92490445264626 23.9126292422843 86-87 22.61798561500351 26.567639340999182 26.848190466480727 23.966184577516586 88-89 22.47172020703063 26.639885105707666 26.390275859787938 24.498118827473768 90-91 22.194560422653918 26.603225606367054 26.874590292532652 24.32762367844638 92-93 22.683135757633707 26.13748515860311 27.07430123247162 24.10507785129156 94-95 22.31069017455968 25.864574781287843 27.263093343004265 24.56164170114821 96-97 22.85227665261674 25.46388735937679 26.79421886923491 24.889617118771557 98-99 22.602433387896458 25.925440110668717 26.33574145883642 25.13638504259841 100-101 22.997343589279485 25.628912147119053 26.521741903292316 24.852002360309147 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3358.0 1 2164.5 2 1130.5 3 1319.5 4 1190.5 5 1096.5 6 1562.0 7 2854.5 8 4414.5 9 4842.5 10 3945.0 11 3057.0 12 2785.0 13 2718.5 14 2746.0 15 2815.0 16 2815.0 17 2920.5 18 3143.0 19 3347.5 20 3667.0 21 4204.0 22 4961.0 23 5699.5 24 6625.0 25 7953.0 26 9394.0 27 11203.0 28 12904.5 29 14575.5 30 16612.5 31 19628.5 32 24229.5 33 25482.5 34 23974.0 35 24476.5 36 26637.5 37 29121.5 38 31480.0 39 33363.0 40 35097.5 41 37662.5 42 39734.5 43 42259.5 44 45583.0 45 49183.0 46 52312.5 47 55192.0 48 58455.5 49 59349.0 50 59387.5 51 60192.5 52 61493.0 53 63937.5 54 65518.0 55 64335.5 56 61406.5 57 57841.5 58 54344.0 59 48716.5 60 40679.0 61 33956.5 62 28216.0 63 21509.0 64 15740.0 65 12251.0 66 9444.5 67 7276.0 68 5600.5 69 4057.5 70 2871.0 71 2133.5 72 1654.5 73 1333.5 74 1093.5 75 876.5 76 672.0 77 492.0 78 337.5 79 243.5 80 176.5 81 130.5 82 87.0 83 51.5 84 40.5 85 30.5 86 21.0 87 10.5 88 7.0 89 7.0 90 5.0 91 3.5 92 3.0 93 2.0 94 1.5 95 0.5 96 0.5 97 0.5 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13300979925630751 2 0.11841928418085458 3 0.12532102160243813 4 0.13567362773481345 5 0.12332315024355868 6 0.13797420687534132 7 0.1680633603711924 8 0.15432042708435498 9 0.16473357477306 10-11 0.1592545639252239 12-13 0.14805437600423307 14-15 0.15301878362326685 16-17 0.14835708378588147 18-19 0.162644891079686 20-21 0.16782119414587368 22-23 0.19978713588794483 24-25 0.22015936959288224 26-27 0.23281255486578542 28-29 0.22769679335592746 30-31 0.21916043391344253 32-33 0.22049234815269547 34-35 0.20980676346050695 36-37 0.21807068589950826 38-39 0.20677968564402294 40-41 0.21150192703773799 42-43 0.20478181428514344 44-45 0.19833413853603252 46-47 0.21162301015039733 48-49 0.24834146406434843 50-51 0.25033933542322784 52-53 0.2516712496624808 54-55 0.2502485230887333 56-57 0.2235194259691795 58-59 0.22448809087045438 60-61 0.19788007686355993 62-63 0.19067563166032797 64-65 0.19170483811793254 66-67 0.19930280343730739 68-69 0.19893955409932934 70-71 0.2040553156091873 72-73 0.23396284443604937 74-75 0.2359001742385991 76-77 0.2500063568634146 78-79 0.24658575893078769 80-81 0.2611460032280758 82-83 0.26005625521414155 84-85 0.2351434047844781 86-87 0.2379283163756434 88-89 0.24110674808295163 90-91 0.44146902875602845 92-93 0.5941245630413172 94-95 0.33827594599208843 96-97 0.2746770410677593 98-99 0.305220256236083 100-101 0.32471463737424006 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1651758.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.34345645131809 #Duplication Level Percentage of deduplicated Percentage of total 1 75.61741794325577 38.06842187187416 2 12.47813688970172 12.563850822005683 3 4.728571618402267 7.141579180439196 4 2.3060753675968026 4.643832193682679 5 1.3229738658386523 3.330153860054006 6 0.8314589996369963 2.511511196356898 7 0.5248124850029479 1.8494612138697752 8 0.3684773764616425 1.4840339804154112 9 0.2798920280831772 1.268165891418888 >10 1.3795078963496057 12.234852989757723 >50 0.09214400898616296 3.1743047355465 >100 0.06174624654625542 5.782646761794749 >500 0.00519692272947925 1.9016839974798718 >1k 0.0032171426420585835 2.6450276538585182 >5k 2.4747251092758333E-4 0.7768573484201875 >10k+ 1.2373625546379166E-4 0.6236163030257795 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10011 0.606081520416429 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7222 0.43723111981295076 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5249 0.3177826291744917 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2378 0.14396782095197966 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2373 0.14366511317033126 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2330 0.141061826248155 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2312 0.13997207823422075 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2166 0.13113301101008742 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2135 0.12925622276386733 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1825 0.11048834030166646 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 1792 0.10849046894278701 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 1767 0.106976930034545 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 1751 0.10600826513327012 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1658 0.10037790039460986 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.0270778164840128E-5 0.0 18-19 0.0 0.0 0.0 9.081233449452039E-5 0.0 20-21 0.0 0.0 0.0 3.329785598132414E-4 0.0 22-23 0.0 0.0 0.0 5.448740069671223E-4 0.0 24-25 0.0 0.0 0.0 0.0017254343553958873 0.0 26-27 0.0 0.0 0.0 0.0034205979326269345 0.0 28-29 0.0 0.0 0.0 0.010655313914023724 0.0 30-31 0.0 0.0 0.0 0.03399408387911546 0.0 32-33 0.0 0.0 0.0 0.08076243614379346 0.0 34-35 0.0 0.0 0.0 0.14436134106812257 0.0 36-37 0.0 0.0 0.0 0.2494009413001178 0.0 38-39 0.0 0.0 0.0 0.4143464115203317 0.0 40-41 0.0 0.0 0.0 0.5935191474780204 0.0 42-43 0.0 0.0 0.0 0.7721772802069068 0.0 44-45 0.0 0.0 0.0 0.9898544459902722 0.0 46-47 0.0 0.0 0.0 1.24776147595471 0.0 48-49 0.0 0.0 0.0 1.5257683026206017 0.0 50-51 0.0 0.0 0.0 1.841916309774192 0.0 52-53 0.0 0.0 0.0 2.1272183939778104 0.0 54-55 0.0 0.0 0.0 2.42641476535909 0.0 56-57 0.0 0.0 0.0 2.765750188586948 0.0 58-59 0.0 0.0 0.0 3.1281216739982494 0.0 60-61 0.0 0.0 0.0 3.5289673184570622 0.0 62-63 0.0 0.0 0.0 3.9057174234966623 0.0 64-65 0.0 0.0 0.0 4.275989582008987 0.0 66-67 0.0 0.0 0.0 4.7070454630763106 0.0 68-69 0.0 0.0 0.0 5.185414570415279 0.0 70-71 0.0 3.0270778164840128E-5 0.0 5.679494211621799 0.0 72-73 0.0 6.0541556329680256E-5 0.0 6.175813890412519 0.0 74-75 0.0 6.0541556329680256E-5 0.0 6.64507149352387 0.0 76-77 0.0 6.0541556329680256E-5 0.0 7.1390905931740605 0.0 78-79 0.0 6.0541556329680256E-5 0.0 7.687930072080777 0.0 80-81 0.0 6.0541556329680256E-5 0.0 8.246910261672713 0.0 82-83 0.0 6.0541556329680256E-5 0.0 8.794327014005685 0.0 84-85 0.0 6.0541556329680256E-5 0.0 9.318283913260903 0.0 86-87 0.0 6.0541556329680256E-5 0.0 9.834128243967942 0.0 88-89 6.0541556329680256E-5 6.0541556329680256E-5 0.0 10.361021408705149 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4930 0.0 42.818645 1 GTATCAA 8780 0.0 40.251457 1 TATCAAC 11045 0.0 32.298706 2 ATCAACG 11385 0.0 31.211035 3 TCAACGC 11510 0.0 30.872078 4 CAACGCA 11715 0.0 30.249939 5 AACGCAG 12155 0.0 29.232979 6 GTACATG 8730 0.0 26.408388 1 TACATGG 8930 0.0 26.402067 2 TAAGGTA 415 0.0 26.292938 4 ACGCAGA 13340 0.0 26.280571 7 AGGTAAG 810 0.0 25.769184 2 CGCAGAG 13625 0.0 25.764107 8 CGTATCA 185 9.100404E-9 25.641777 1 TAAGGTG 905 0.0 25.161634 5 AAGGTAA 815 0.0 25.028263 1 ACATGGG 9280 0.0 24.641008 3 GCAGAGT 14050 0.0 23.97114 9 GGTAAGG 995 0.0 23.363201 3 GTGTAAG 535 0.0 23.053637 1 >>END_MODULE