FastQCFastQC Report
Fri 27 May 2016
SRR939737_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939737_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1651758
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106580.6452519073617322No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84990.5145426872459524No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67820.4105928350278915No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA28110.1701823148427312No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27410.1659444058996536No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG26020.15752912956982804No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25010.15141443238053032No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC24800.15014305969760702No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24640.14917439479633215No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22170.13422063038290113No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20180.12217286067329476No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG19030.11521058169538152No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA18120.10970130006938063No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18030.1091564260624135No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT17160.10388931066173132No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT17100.10352606132375325No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA17050.10322335354210485No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA16910.10237577175348932No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52450.046.1871261
GTATCAA93700.039.3342131
AGGTAAG7700.032.4336932
TATCAAC123000.029.9963132
ATCAACG124450.029.5305463
TCAACGC124600.029.3815544
CAACGCA127250.028.6956235
AACGCAG130750.027.948286
GGTAAGG10050.027.6603553
TAAGGTG10750.027.6123815
GTACATG87750.026.8550641
TACATGG88900.026.237032
AAGGTAA9100.025.3780361
ACGCAGA146300.024.9455137
CGCAGAG147450.024.7509578
ACATGGG90800.024.2327583
GTAAGGT11500.024.1727454
GCAGAGT154550.023.034989
AGTACTT95200.021.71514112-13
AGAGTAC139750.021.48144110-11
Produced by FastQC (version 0.11.5)