FastQCFastQC Report
Fri 27 May 2016
SRR939736_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939736_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1770947
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107660.6079233314153388No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77400.43705429919698335No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57390.3240639047921818No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA26790.15127499580732795No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26420.14918571814966794No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25180.14218381464832092No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24720.13958633431717604No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22700.1281800076456269No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG22320.12603426302424636No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC19540.1103364471099361No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA19310.10903770694436365No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG19100.10785190070623232No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA18900.1067225614318215No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57250.042.8871
GTATCAA99500.039.8033561
TAAGGTA4800.031.3617844
TATCAAC127150.031.0385062
TCAACGC129500.030.514174
ATCAACG129850.030.504383
CAACGCA132350.029.8206965
AACGCAG136750.028.9308196
GTACATG97350.027.9752041
TACATGG98350.027.405362
ACATGGG98150.026.1215083
ACGCAGA151300.025.8377177
AGGTAAG8800.025.6574672
CGCAGAG152300.025.451858
GCAGAGT155150.023.5895619
TAAGGTG11350.023.2098275
AAGGTAA9300.022.7593691
GAGTACT88100.022.64125312-13
GTGTAAG6450.022.6064641
GTAAGGT11900.022.5330494
Produced by FastQC (version 0.11.5)